24 results about "Dialyzates" patented technology

A hydraulic system and method are provided for breaking-up, dislodging, removing, and preventing the build-up of biofilm in a dialysate pathway of an extracorporeal blood treatment device. The dialysate pathway can include a dialyzer discharge line, a drain line, a dialyzer feed line, and a bypass system. At least one ultrasonic device can be positioned and configured to generate ultrasonic waves in the dialysate pathway and to propagate the ultrasonic waves along at least a portion of the dialysate pathway. The ultrasonic waves can be used to break-up biofilm. An ionizing electrode pair can also, or instead, be implemented to break-up, dislodge, remove, and prevent a build-up of biofilm. The system and method can particularly be implemented and useful in non-disposable portions of a hydraulic system of an extracorporeal blood treatment device.

This invention relates to the field of carbon nanomaterial preparation technology, specifically a high-yield preparation method for carbon quantum dots and its application. The preparation method includes: adding citric acid, ethylenediamine, EDC, and NHS to deionized water, stirring at 60-90°C for a preset time to obtain a mixed solution; subjecting the mixed solution to a hydrothermal reaction, cooling to room temperature after the reaction is complete, and then centrifuging, filtering, and dialyzing; freeze-drying the dialysate to obtain carbon quantum dots. This invention combines EDC and NHS pretreatment with a hydrothermal method to prepare carbon quantum dots. The solvent used is water, which is non-toxic, harmless, safe, and reliable. The process is simple, safe, and environmentally friendly. Simple pretreatment can greatly improve the yield of carbon quantum dots while ensuring quantum efficiency. The preparation equipment is simple, the process is safe, and it consumes fewer resources, which is conducive to large-scale production.

The invention provides a lactobacillus fermentation extraction method of marine red algae polysaccharide and application of the marine red algae polysaccharide, and the method comprises the following steps: mixing marine red algae powder with water, and carrying out pasteurization at 60-70 DEG C for 20-40 minutes; adding lactobacillus plantarum into the sterilized marine red algae turbid liquid, and performing fermentation culture to obtain a marine red algae fermentation mixed liquid; heating and extracting the marine red algae fermentation mixed solution at 85-95 DEG C for 1.5-2.5 hours, centrifuging, collecting supernate, carrying out rotary evaporation and concentration to 1 / 5 of the original volume, then adding ethanol, standing overnight, discarding the supernate, adding distilled water into precipitate for redissolving, and deproteinizing by using a Sevag method to obtain a polysaccharide solution; and dialyzing the polysaccharide solution, and concentrating and freeze-drying the dialysate to obtain the marine red algae polysaccharide. According to the method, the low-molecular-weight modified polysaccharide is obtained through lactobacillus plantarum fermentation, extraction and purification, and the low-molecular-weight modified polysaccharide is high in sulfate group content, loose in structure, capable of remarkably inhibiting AGEs-induced intestinal cell oxidative stress and inflammatory reaction, capable of being used for preparing the glycosylation injury resisting medicine and good in industrialization prospect.

The dialysate barrel feeding device comprises a first conveyor and a second conveyor which are sequentially arranged in the dialysate barrel conveying direction, the discharging end of the first conveyor is in butt joint with the feeding end of the second conveyor, and the conveying speed of the first conveyor is smaller than that of the second conveyor; the barrel separating and clamping assembly is located in front of the joint of the first conveyor and the second conveyor and comprises a pair of oppositely-arranged clamping pieces, the clamping pieces are located on the two sides of the first conveyor correspondingly, and the distance between the clamping pieces is smaller than the width of a dialysate barrel; friction force between the first conveyor and the dialysate barrel and friction force between the second conveyor and the dialysate barrel can drive the dialysate barrel to leave the clamping piece; the distance between the contact position of the clamping piece and the dialysate barrel and the joint of the first conveyor and the second conveyor is smaller than the length of the dialysate barrel. The dialysate barrel separating and counting device can accurately and effectively separate and count dialysate barrels.

The utility model relates to a directional feeding device for a dialyzer end cover, a dialysate flow opening of the end cover is of a circumferential protruding structure, the directional feeding device comprises a straight vibration feeding assembly, a static material channel assembly, an end cover transferring mechanism and an end cover directional mechanism, the static material channel assembly is arranged in the conveying direction of the straight vibration feeding assembly, and the end cover directional mechanism is arranged on the end cover transferring mechanism. The end cover transferring mechanism clamps and moves the end cover, and a stop block and a deflector rod in the end cover orientation mechanism are matched to limit the rotation angle of the dialysate flow opening, so that the dialysate flow opening is forcibly corrected to the preset direction. The end cover feeding device solves the problem that in the prior art, when end covers are fed, the directions of dialysate flow openings of the end covers are inconsistent. The device can greatly improve the production efficiency and reduce the time and cost of manual adjustment.

The application provides a preparation method of selenium-doped Prussian blue nanoparticles modified by a targeting polypeptide, and comprises the following steps: (1) synthesizing Prussian blue nanoparticles by a microwave method; (2) synthesizing selenium-containing Prussian blue by a gas-phase atomic deposition method; and (3) preparing selenium-doped Prussian blue nanoparticles modified by a targeting polypeptide: dissolving a tumor-targeting RGD cyclic peptide in ultrapure water, adding a catalyst N-hydroxysuccinimide and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and then stirring, then performing dialysis, adding the selenium-containing Prussian blue into the dialysate, stirring, centrifuging, resuspending and obtaining the selenium-doped Prussian blue nanoparticles modified by the targeting polypeptide. According to the preparation method, the selenium is naturally deposited on the surface of the Prussian blue by the gas-phase atomic deposition method, monodisperse selenium with controllable doping amount is formed, and the selenium-doped Prussian blue modified by the targeting polypeptide is prepared.

The invention discloses selenium-enriched spirulina carbon dots as well as a preparation method and application thereof, and belongs to the technical field of medicines. The selenium-rich spirulina powder is obtained by taking selenium-rich spirulina growing to a logarithmic phase as a raw material, centrifuging, collecting and freeze-drying. Uniformly mixing the spirulina platensis with a catalyst and deionized water according to a mass ratio of 1: (0.1-0.8): 20, then putting the mixture into a high-temperature reaction kettle to react at 140-260 DEG C for 1.5-5.5 hours, dialyzing the reaction liquid for 24 hours after the reaction is finished, centrifuging, and freeze-drying the dialysate to obtain the selenium-enriched spirulina platensis carbon dots with the yield of 70-95%. The selenium-enriched spirulina carbon dots provided by the invention not only have the inhibition capability of alpha-amylase, alpha-glucosidase, cholesterol esterase and pancreatic lipase and the cholate binding capability, but also can improve the endogenous antioxidant defense capability of model organisms while removing exogenous oxidizing substances, so that the glycolipid metabolism balance is efficiently regulated and controlled.

This invention proposes a method for synthesizing T-2 toxin antigen and preparing and applying monoclonal antibodies, relating to the field of biotoxin detection technology. The method for preparing T-2 toxin antigen includes the following steps: First, the immunogen T-2-BSA is synthesized using the carbodiimide method. 2 mg of T-2 adipic acid is dissolved in 0.5 mL of dimethylformamide, denoted as solution A; 5 mg of bovine serum albumin and 3 mg of EDC are dissolved in a 2-morpholine ethanesulfonic acid buffer solution at pH 5.5, denoted as solution B. Second, solution A is added dropwise to solution B under stirring. The mixture is stirred at 4°C in the dark for 18 hours, then dialyzed with phosphate buffer solution for 3 days, changing the solution daily. The dialysate is centrifuged at 3500 r / min for 10 min, and the precipitate is discarded. The unique step of chemically conjugating adipic acid to T-2 toxin not only enriches the structural characteristics of the hapten, but also results in a complete T-2 toxin antigen that exhibits excellent immunogenicity, efficiently stimulating the body's immune response and demonstrating high specificity and sensitivity.

The invention discloses a preparation method and application of a gleditsia sinensis saponin-silver ion antibacterial chelate, and belongs to the technical field of deep processing of natural products. The method comprises the following steps: (1) extracting gleditsia sinensis saponin by using a deep eutectic solvent, dialyzing an extracting solution, purifying by using macroporous resin, and drying to obtain high-purity gleditsia sinensis saponin; and (2) fully dissolving the prepared gleditsia sinensis saponin as a raw material in distilled water, adding silver nitrate to perform an ultrasonic chelation reaction, dialyzing after chelation is completed, centrifuging dialysate, taking supernatant, and freeze-drying to obtain the gleditsia sinensis saponin-silver ion antibacterial chelate which can effectively inhibit escherichia coli and staphylococcus aureus. The method is easy to operate, the efficiency of chelating the gleditsia sinensis saponin with the silver ions is high, the chelate is good in stability, and the gleditsia sinensis saponin has huge potential in the application aspect of serving as a green, environment-friendly, safe and efficient antibacterial functional surfactant.

The application belongs to the technical field of magnetic resonance imaging, and provides a magnetic resonance contrast agent based on active protein targeting, which comprises 3-6 parts of fibroblast activation protein targeting molecules, 6-8 parts of ferritin, 6-15 parts of KGDS tetrapeptide, 28-42 parts of dialysate, and 15-30 parts of paramagnetic metal salt, so that the application can realize long circulation in vivo, enhance targeting, reduce non-specific accumulation, reduce damage to normal tissues, and enhance the contrast effect.

The present invention aims to provide a method for measuring endotoxin concentration of dialysate with higher accuracy using a calibration curve made of water, and a kit for the method. The present invention provides a method for measuring endotoxin as follows: mixing a prescribed amount of a test sample with a dry powder containing a solubilizer reagent to prepare a first reaction solution, then incubating the first reaction solution for a prescribed time, mixing the first reaction solution with a dry powder containing a luciferase bioluminescence reagent under light shielding to prepare a second reaction solution, measuring the amount of luminescence of the second reaction solution, and measuring the endotoxin concentration of the test sample, which is dialysate or water, from the amount of luminescence, the second reaction solution having a sodium ion concentration of 250 to 390 mM, a calcium ion concentration of 0.5 to 10 mM, a chloride ion concentration of 250 to 361.5 mM, and a Tris concentration of 20 to 30 mM.

Apparatus (1) and method for producing a dialysate, the apparatus (1) comprising a forward osmosis (FO) unit (2) including a feed side (2a) and a draw side (2b) separated by a FO membrane (2c), the feed side (2a) being included in a feed liquid path (3) and the draw side (2b) being included in a draw liquid path (4), the FO unit (2) being configured to receive a dialysis concentrate solution at the draw side (2b) and a spent dialysate solution at the feed side (2a), such that an osmotic pressure difference between the draw side (2b) and the feed side (2a) transports water from the spent dialysate solution through the FO membrane (2c) to the dialysis concentrate solution, thereby diluting the dialysis concentrate solution to a dilute dialysis concentrate solution and dehydrating the spent dialysate solution to a dehydrated spent dialysate solution, and providing (S3) a hydrostatic pressure difference between the draw side (2b) and the feed side (2a) using one or more pressure pumps (7, 32). The apparatus (1) is configured to sense one or more characteristics of the diluted dialysis concentrate solution and / or the dehydrated spent dialysate, sense one or more pressures indicative of a hydrostatic pressure difference between the draw side (2b) and the feed side (2a), and control at least one of a flow rate of the spent dialysate to the feed side (2a), a flow rate of the dialysis concentrate solution to the draw side (2b), and the hydrostatic pressure difference based on the one or more characteristics of the diluted dialysis concentrate solution and / or the dehydrated spent dialysate and the sensed one or more pressures indicative of the hydrostatic pressure difference to produce a diluted dialysis concentrate solution.

The utility model discloses a dialysate filter convenient to support, fix and use, and relates to the technical field of dialysate filters. Comprising a filter box, a filter plate is movably arranged in the filter box, a transfer pump is fixedly arranged on the other side of the filter box, a transfer pipe is fixedly arranged on one side of the transfer pump, a storage box is fixedly arranged at one end of the transfer pipe, and a box cover is movably arranged at the top of the storage box; a filter box is fixedly arranged at the top of the box cover, a connecting pipe is fixedly arranged at the bottom of the filter box, a spray head is fixedly arranged at one end of the connecting pipe, a filter screen is fixedly arranged in the filter box, and a threaded rod is movably arranged in the filter box and located above the filter screen; and a nut seat is movably arranged outside the threaded rod, a connecting rod is fixedly arranged at the bottom of the nut seat, and a cleaning brush is fixedly arranged at one end of the connecting rod.

This invention discloses a method for modifying the structure of recombinant mussel adhesive protein, comprising the following steps: S1. Optimizing the mussel adhesive protein gene sequence and cloning it into an expression vector to construct a recombinant plasmid; S2. Transforming the recombinant plasmid into competent cells and culturing them to obtain engineered bacteria; S3. Fermenting the engineered bacteria, collecting the bacterial cells by centrifugation, adding a lysis solution to break the bacterial cells, purifying and collecting the eluent, and dialysis to obtain a dialysate containing non-adhesive recombinant mussel adhesive protein; S4. Adding a chemical oxidant and an enhancer to the dialysate containing the non-adhesive recombinant mussel adhesive protein to react and obtain a highly adhesive recombinant mussel adhesive protein. This invention provides a novel chemical structure modification method for recombinant mussel adhesive protein. Through the synergistic effect of highly selective oxidants and enhancers, the tertiary structure of the protein is unfolded and the reaction activation energy is reduced, achieving efficient and simultaneous tyrosine hydroxylation and DOPA quinone crosslinking. The modification rate is more than 7 times higher than that of traditional chemical methods.

The utility model relates to an aluminum paper opener for a dialysate barrel. The aluminum paper opener comprises a handle, a tray fixedly arranged on the handle, a cutting structure arranged on the tray and a fixing structure arranged on the tray. The cutting structure comprises a rotating rod rotationally arranged on the tray, a first rotary knob fixedly arranged at one end of the rotating rod, a plurality of cutting knives fixedly arranged at the other end of the rotating rod, a limiting ring fixedly arranged on the rotating rod and a plurality of arc-shaped clamping plates fixedly arranged at the ends, away from the tray, of the cutting knives correspondingly. The multiple arc-shaped clamping plates and the multiple cutting knives are arranged in an inclined mode. By using the opener, aluminum paper can be conveniently and rapidly cut, medical staff can conveniently use the opener, and dialysate in a barrel cannot be polluted.

The application discloses a method for preparing Gp32 protein and detecting the affinity of the Gp32 protein. The method for preparing the Gp32 protein comprises purifying the Gp32 protein by using a protein purification system of Buffer A, Buffer B and dialysate provided by the application. The method for detecting the affinity of the Gp32 protein comprises detecting the affinity of the Gp32 protein by using a system for detecting the affinity of the Gp32 protein and ssDNA provided by the application. The protein purified by using the purification method provided by the application has high purity, good thermal stability and high affinity with ssDNA.

The utility model relates to a dialysate sample elevator which comprises an elevator main body, a sample lifting assembly and a sample clamping assembly, the sample lifting assembly is installed in the elevator main body, and the sample clamping assembly is installed on the inner wall of the top end of the elevator main body; the sample lifting assembly comprises a first motor, a first gear, a screw rod, a second gear, a guide rail, a toothed belt, an annular sliding belt, a lifting block, a threaded hole, a connecting square rod, a connecting belt and a lifting plate; the sample lifting assembly is adopted, the practical operation process can be simplified, a worker does not need to keep the device to reach a designated position and can leave, the sample can be automatically lifted, the manual burden is reduced, and the using effect of the device is improved; the sample clamping assembly is adopted, when the sample is lifted, the sample can be automatically clamped, so that when the sample is lifted, the sample is prevented from shaking, it is ensured that the sample can be stably conveyed, and the lifting effect of the sample is improved.

The present invention relates to a device for dialysis treatment, in particular a device for peritoneal dialysis, having first and second discontinuous pumps that are switchable by means of at least two valves and having a control for generating a continuous volume flow of dialyzate, characterized in that the control is adapted to carry out at least one of the following steps: a) suspending at least one pressure measurement during a switching time of at least one of the valves; b) calculatory compensation of a portion of a result of a pressure measurement based on unit properties during a switching time of at least one of the valves; c) postponing a valve switching point in time of the second pump so that the valve switching point in time of the second pump does not coincide with a valve switching point in time of the first pump; and d) adaptive postponement of a valve switching point in time of the second pump so that said valve switching point in time does not coincide with a valve switching point in time of the first pump, wherein use is preferably made by the control of an optimization algorithm for determining a point in time to which the valve switching point in time of the second pump is postponed.

The invention belongs to the technical field of organic probe molecule analysis and detection, and discloses a light-operated electrochemical molecular probe (TPMP), a preparation method thereof and application of the light-operated electrochemical molecular probe in detection of copper ions (Cu < 2 + >). The molecular structural formula of the probe is as follows: hydroquinone and 2-nitrobenzyl bromide react to prepare a compound 1, and then the compound 1 and pyridine formyl chloride hydrochloride are subjected to esterification reaction under the catalysis of triethylamine to obtain a target product. The probe realizes light-operated open-type electrochemical detection of Cu < 2 + > based on a sequential response mechanism activated by Cu < 2 + > specific recognized-365 nm ultraviolet light, has no electrochemical signal in darkness, quantitatively releases electroactive substances after light activation and generates significant response, has a detection limit as low as 30 nM, has ultrahigh selectivity on Cu < 2 + >, and can effectively resist biological matrix interference. The fluorescent probe is applied to quantitative detection of free Cu < 2 + > in a hippocampal dialysate of an AD (Alzheimer's disease) model mouse.

The present invention relates to a dialysate composition having a composition allowing equilibrium with the blood, the composition comprising a mineral salt and a pH buffer and comprising at least one iron oxide nanoparticle (Fe2 + and / or Fe3 +) coated with at least one polymeric compound.