18 results about "Endogenous hormone" patented technology

This invention discloses a method for delaying leaf senescence in Paspalum notatum under salt stress. The method includes the following steps: plant material culture and salt stress treatment; H2O2 content determination; DAB staining; chlorophyll content and Fv / Fm value determination; lipidomics data determination and analysis; endogenous hormone level analysis; real-time quantitative PCR analysis of gene expression levels; and transcriptomics data analysis. This invention discovers that root-derived H2O2 in Paspalum notatum under salt-driven conditions delays leaf senescence by participating in the regulation of jasmonic acid. The role of root-derived H2O2 was studied using diphenylthioiodine (DPI), a specific inhibitor of NADPH oxidase. Furthermore, the influence of the presence or absence of root-derived H2O2 on the expression of genes related to jasmonic acid synthesis precursors was investigated at the gene expression level. Mass spectrometry-based omics technology and data analysis are the first key technologies for implementing this project. Lipidomics, as one of the most important branches of metabolomics, can perform qualitative and quantitative analysis at the level of individual lipid species.

This invention discloses a peony PsLBD12 Genes and their application in regulating root development PsLBD12 The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. This invention was cloned from the peony cultivar 'Zhengwu'. PsLBD12 The gene sequence was obtained and its expression was analyzed in peony. Subsequently, an overexpression vector was constructed and introduced into the target plant to verify its function. It was found that overexpression... PsLBD12 Compared with wild-type Arabidopsis seedlings, the Arabidopsis seedlings with this gene grew faster, exhibited earlier stem elongation, and showed earlier senescence of basal leaves in the later stages of growth. The taproot length was significantly shortened, the number of lateral roots was significantly increased, and the endogenous hormones IAA and ABA in the leaves were significantly increased. The average number of roots in detached leaves was significantly increased on MS medium containing IAA, and the average root length was significantly increased on MS medium containing IBA. It is evident that this gene will have wide applications in the cultivation and genetic improvement of peonies and other horticultural plants.

The application discloses a bacillus thuringiensis and application thereof. The bacillus thuringiensis ZF505 provided by the application has a preservation number of CGMCC No.24585 in the China General Microbiological Culture Collection Center. A potting living body test shows that the bacillus thuringiensis can prevent and treat cucumber downy mildew with a highest effect of 73.01%, and the bacillus thuringiensis CGMCC No.24585 can promote the increase of the content of three endogenous hormones of cucumber, wherein gibberellin is increased by 15.61%, indole acetic acid is increased by 5.05%, and zeatin is increased by 5.12%.

The application provides a use of a banana tissue endogenous hormone zeatin in resisting banana wilt, and belongs to the technical field of plant protection. The banana tissue endogenous hormone is zeatin, and experiments prove that in vitro tests (tissue culture bottles and greenhouses) of the zeatin or lutein show that the zeatin can effectively inhibit the growth of TR4 mycelium, exhibits good anti-banana wilt fungus activity, has a significant prevention and control effect on the banana wilt fungus (TR4), and does not affect the growth of seedlings. The application provides a new idea and method for preventing and controlling TR4, and lays a foundation for in-depth exploration of the prevention and control of the banana wilt disease TR4.

The invention discloses a method for testing and analyzing the content of endogenous hormones in angelica sinensis. The method for testing the content of the endogenous hormones comprises the following steps: in an enzyme-linked immunosorbent assay (ELISA), homogenizing a sample with a 80% methanol solution, extracting at 4 DEG C for 2-10 hours, centrifuging to take supernate, purifying, blow-drying with nitrogen, and dissolving to a constant volume. The liquid chromatography-tandem mass spectrometry (LC-MS) method comprises the following steps: extracting a sample with 80% methanol, filtering, carrying out rotary evaporation, washing with a methanol-water-acetic acid mixed solution, eluting, collecting an eluent, blow-drying with nitrogen, dissolving and feeding the sample. If the result difference value of the two methods is less than or equal to 15%, completing ELISA method result correction; if the difference value is large, adjusting the sample amount and the extraction condition until the difference value is less than or equal to 15%. According to the method, the content of the endogenous hormone is measured by utilizing an ELISA method and an HPLC-MS / MS method, so that the hormone content is rapidly and accurately detected while the operation is simple and convenient.

The invention discloses an evaluation method and application of mesosulfuron-methyl herbicide-resistant wheat, and belongs to the technical field of wheat breeding. The method comprises the following steps: performing endogenous hormone content detection, specific metabolite content detection, wheat plant height, flag leaf form, grain yield and quality index detection on to-be-evaluated wheat sprayed with mesosulfuron-methyl, and comparing a detection result with a non-pesticide-applied control or a known variety; and comprehensively evaluating the tolerance of the wheat to mesosulfuron-methyl. According to the invention, a set of multi-index comprehensive evaluation system is constructed. The method effectively overcomes the defects that a traditional method depends on subjective field observation, evaluation lags behind and indexes are single, achieves objective identification of mesosulfuron-methyl resistance of wheat varieties, and provides reliable technical support for rapid screening of resistant varieties, early evaluation of breeding materials and safe field medication. The invention also provides application of the method in preparation of a detection kit.

The application belongs to the technical fields of plant molecular biology and genetic engineering, and relates to a poplar PtoNAC43 transcription factor gene and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO:1, and the gene encodes a transcription factor protein with a NAC domain. A transgenic plant is obtained by constructing an overexpression vector and introducing the vector into a poplar. The results show that overexpression of the gene can reduce plant height, biomass and growth potential, and affect photosynthesis parameters, endogenous hormone levels and tissue structure. The application can be used for plant growth regulation and dwarf plant cultivation.

The invention relates to an application of urolithin A in preparation of a product for improving an endogenous estrogen level. The molecular formula of the urolithin A is C13H8O4, and the structural formula of the urolithin A is shown as a formula (I). The improvement of the endogenous estrogen level comprises the following steps: selectively regulating and controlling synthesis pathways of adrenal hormone and ovarian estrogen, and synergistically improving the in-vivo estrogen level. Besides, the urolithin A has good hormone regulation selectivity, can effectively inhibit cortisol secretion, and has no obvious influence on aldosterone level, so that blood pressure regulation and body fluid homeostasis are prevented from being interfered. The action characteristics lay a foundation for the application of the urolithin A in improvement of female climacteric hormone disorder and related health support directions, and the urolithin A has a wide prospect as an endogenous hormone regulation strategy. (I)

The invention discloses a microbial composition with a flowering promoting effect and application of the microbial composition in regulation and control of the flowering time of corn, and belongs to the technical field of agricultural microorganisms. The microbial composition comprises Ensifer strain S281 and bacillus subtilis; a method for promoting precocious flowering of corn comprises the following steps: culturing S281 in an enrichment culture medium to prepare a microbial inoculum; and mixing with a bacillus subtilis inoculant according to a volume ratio of 1: 1, inoculating the mixture into the rhizosphere soil of the corn, and maintaining the corn until the corn blooms. The microbial composition disclosed by the invention can enhance rhizosphere nitrification and regulate endogenous hormone level through the Ensifer strain S281, and can generate a synergistic effect with bacillus subtilis, so that crops such as corn and the like can be obviously promoted to head in advance, and the flowering time can be 2-7 days earlier than that of an untreated control group; and a new microbial solution is provided for realizing green and accurate regulation and control of the growth period of crops.

The invention provides a screening method for allyl isothiocyanate tobacco suckercide matching compounds, and belongs to the technical field of tobacco suckercide, the screening method comprises the following steps: firstly, establishing a tobacco tissue culture model to obtain a standardized lateral bud material, then preparing a gradient mixed solution of allyl isothiocyanate and a to-be-screened compound, and then preparing a tobacco suckercide compound; the method comprises the following steps: accurately applying a solution to the base parts of lateral buds by adopting a trace application technology, constructing a multi-dimensional evaluation system containing the length of the lateral buds, biomass and endogenous hormone content, calculating a comprehensive bud inhibition index, and introducing a component bud inhibition contribution function to quantitatively evaluate a formula effect. A weight optimization network is constructed by combining a maximum flow minimum cut algorithm to realize automatic optimization of a weight coefficient, proportion adjustment and compound replacement are performed according to a contribution function value, and finally, a compound combination with the best bud inhibition effect and the minimum influence on stem growth is screened out by evaluating plant physiological influence and field verification tests.

The invention belongs to the technical field of molecular genetics, and particularly relates to an application of a ZmNAL4 gene or an encoded protein thereof in regulation and control of leaf morphology and plant height of a plant. The analysis of the expression mode of the flavin monooxygenase gene ZmNAL4 in amphiphilic and transgenic plants shows that the leaf width of an overexpression strain is increased by 13.2% and the plant height is increased by 14.8% compared with that of a gene editing strain, and the leaf area and the plant height of an arabidopsis overexpression plant are obviously higher than those of a wild plant, so that the ZmNAL4 has a positive regulation effect on the leaf width; interaction protein analysis identifies that density-tolerant response factors Zmfdx1 and Zmfdx2 and leaf included angle candidate genes ZmPDHE1 and ZmNAL4 interact with each other; plant endogenous hormone analysis shows that the ZmNAL4 regulates and controls the leaf form and the plant height by influencing an auxin signal and a brassinolide path; gene resources and theoretical basis are provided for research on density-tolerant plant types with reasonable leaf widths of corn, and the method has important significance on density-tolerant high-yield breeding.

The application provides a method for shortening the childhood of Psidium guajava hybrid seedlings, and belongs to the technical field of fruit tree cultivation. In the application, different concentrations of nutrient solution are poured on the Psidium guajava seedlings to cultivate strong plants; after the main stem sprouts, 8-10 times of bud rubbing and pinching are carried out to promote the maturation of new shoots and the rapid expansion of the crown; and exogenous hormones and endogenous hormones are sprayed to promote the early flowering and fruiting of the plants. The method can shorten the childhood of the Psidium guajava hybrid seedlings from 18 months to 11 months, and thus solves the problems of long breeding cycle and low breeding efficiency of Psidium guajava caused by the long childhood of the Psidium guajava hybrid seedlings.

The invention provides a methyl jasmonate tobacco bud inhibition effect evaluation method, which belongs to the technical field of tobacco bud inhibition, and comprises seven key steps of standardized image acquisition, neural network bud segmentation, multi-dimensional feature extraction, endogenous hormone determination, bud inhibition effect quantitative calculation, weight dynamic adjustment and grade division. According to the core technology, based on a deep convolutional neural network of an improved UNet architecture and a multi-head attention mechanism, objective quantitative evaluation of the tobacco bud inhibition effect is achieved by precisely segmenting bud images, extracting key morphological features, combining endogenous hormone measurement data and applying a self-adaptive weight distribution function. The system adopts a closed-loop iterative optimization strategy, the evaluation accuracy is remarkably improved by dynamically adjusting a weight calculation mode, and the technical problem that the accuracy is not enough due to high subjectivity of a traditional method is effectively solved.

The present application belongs to the technical field of fruit tree growth regulation, and relates to a regulation method for promoting branch efficiency of fruit trees, and specifically comprises the following steps: step one, limited root cultivation, optimization of root system configuration and function, laying the material foundation for the whole tree nutrient storage and absorption capacity; step two, precise induction, systematic regulation of endogenous hormone balance and metabolism when the root system is in the best physiological state, and directional activation of lateral bud meristem; step three, incision blockage, physical blockage to inhibit signal flow, and at the same time, guiding the enrichment of nutrients to the target bud site, forming a synergistic effect with the chemical induction of step two; step four, spectrum consolidation, providing precise light signals and energy support during the most critical window period of the differentiation and growth of the new lateral bud, maximizing the results of the previous induction. The present application constructs a four-in-one regulation chain of "root-bud-flow-light", significantly improves the branch efficiency, and lays a solid foundation for building a high-yield and high-quality tree crown.

This invention discloses a highly efficient suspension culture method for Rhododendron simsii cells, belonging to the field of plant cell culture and natural product production technology. This method uses TDZ (thiazuron) instead of traditional 6-BA (6-benzylaminopurine) as a cytokinin, combined with four-stage hormone gradient regulation, LC-MS / MS (liquid chromatography-tandem mass spectrometry) dynamic monitoring of endogenous hormones, and staged suspension culture technology to establish a stable Rhododendron simsii suspension cell line. Compared with existing technologies, this method increases callus induction rate from 60% to 90%, reduces browning rate from 22% to 3%, increases cell proliferation from 2.85 times to 4.25 times, increases flavonoid content from 20.8 mg / g to 38.6 mg / g, increases purity from 72.3% to 93.5%, and reduces resource consumption by 97.8%, achieving efficient, green, and sustainable production of Rhododendron simsii flavonoids.

This invention discloses a peony PsPYL4 Genes and their application in regulating root development PsPYL4 The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. This invention was cloned from the peony cultivar 'Zhengwu'. PsPYL4 The gene sequence was obtained and its expression was analyzed in peony. Subsequently, an overexpression vector was constructed and introduced into the target plant to verify its function. It was found that overexpression... PsPYL4 Compared to wild-type Arabidopsis seedlings, the genetically modified Arabidopsis seedlings... PsPYL4 The overexpression lines showed faster growth, larger rosette leaves, significantly shorter taproot length, and significantly increased lateral root number. The levels of endogenous hormones IAA, ABA, ZR, and GAs in the leaves were increased. The average number of roots in the detached leaves was significantly increased on MS medium containing IAA, and the average root length was significantly increased on MS medium containing IBA. It is evident that this gene will have wide applications in the cultivation and genetic improvement of peonies and other horticultural plants.

This invention discloses a peony PsGH3.1 The application of genes in regulating root development PsGH3.1 The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. This invention was cloned from the peony cultivar 'Zhengwu'. PsGH3.1 The gene sequence was obtained and its expression was analyzed in peony. Subsequently, an overexpression vector was constructed and introduced into a model plant to verify its function. It was found that overexpression... PsGH3.1 Compared with wild-type Arabidopsis seedlings, the Arabidopsis seedlings with this gene exhibited stunted growth, wrinkled leaves, and shorter pods. Simultaneously, the taproot length was significantly shortened, while the number of lateral roots increased significantly. The levels of endogenous hormones IAA, ABA, and ZR in the leaves increased, while GAs showed no significant change. The detached leaves showed a significant increase in the average number of roots on MS medium containing IAA and a significant increase in the average root length on MS medium containing IBA. This indicates that this gene will have wide applications in the cultivation and genetic improvement of peonies and other horticultural plants.

The invention belongs to the technical field of fruit tree growth regulation and control, and relates to a regulation and control method for promoting fruit tree branching efficiency, which specifically comprises the following steps: step 1, root-limited cultivation, root system configuration and function optimization, nutrient storage and absorption capability of a whole tree, and laying of a material basis; step 2, accurate induction: when the physiological state of the root system is optimal, systematically regulating endogenous hormone balance and metabolism, and directionally activating lateral bud meristem; the method comprises the following steps: step 1, carrying out first-stage induction, step 2, carrying out second-stage induction, step 3, inhibiting a signal flow by carving and physical blocking, guiding enriched nutrients to a target bud position, and forming a synergistic effect with chemical induction in the step 2, and step 4, carrying out spectrum consolidation, providing accurate optical signals and energy support in the most critical window period of differentiation and growth of new lateral buds, and maximizing an early-stage induction result. According to the method, a'root-bud-flow-light 'four-in-one regulation and control chain is constructed, the branching efficiency is remarkably improved, and a solid foundation is laid for constructing high-yield and high-quality tree crowns.