11 results about "Endogenous hormone" patented technology

This invention discloses a peony PsLBD12 Genes and their application in regulating root development PsLBD12 The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. This invention was cloned from the peony cultivar 'Zhengwu'. PsLBD12 The gene sequence was obtained and its expression was analyzed in peony. Subsequently, an overexpression vector was constructed and introduced into the target plant to verify its function. It was found that overexpression... PsLBD12 Compared with wild-type Arabidopsis seedlings, the Arabidopsis seedlings with this gene grew faster, exhibited earlier stem elongation, and showed earlier senescence of basal leaves in the later stages of growth. The taproot length was significantly shortened, the number of lateral roots was significantly increased, and the endogenous hormones IAA and ABA in the leaves were significantly increased. The average number of roots in detached leaves was significantly increased on MS medium containing IAA, and the average root length was significantly increased on MS medium containing IBA. It is evident that this gene will have wide applications in the cultivation and genetic improvement of peonies and other horticultural plants.

The application discloses a bacillus thuringiensis and application thereof. The bacillus thuringiensis ZF505 provided by the application has a preservation number of CGMCC No.24585 in the China General Microbiological Culture Collection Center. A potting living body test shows that the bacillus thuringiensis can prevent and treat cucumber downy mildew with a highest effect of 73.01%, and the bacillus thuringiensis CGMCC No.24585 can promote the increase of the content of three endogenous hormones of cucumber, wherein gibberellin is increased by 15.61%, indole acetic acid is increased by 5.05%, and zeatin is increased by 5.12%.

The application provides a use of a banana tissue endogenous hormone zeatin in resisting banana wilt, and belongs to the technical field of plant protection. The banana tissue endogenous hormone is zeatin, and experiments prove that in vitro tests (tissue culture bottles and greenhouses) of the zeatin or lutein show that the zeatin can effectively inhibit the growth of TR4 mycelium, exhibits good anti-banana wilt fungus activity, has a significant prevention and control effect on the banana wilt fungus (TR4), and does not affect the growth of seedlings. The application provides a new idea and method for preventing and controlling TR4, and lays a foundation for in-depth exploration of the prevention and control of the banana wilt disease TR4.

The invention discloses an evaluation method and application of mesosulfuron-methyl herbicide-resistant wheat, and belongs to the technical field of wheat breeding. The method comprises the following steps: performing endogenous hormone content detection, specific metabolite content detection, wheat plant height, flag leaf form, grain yield and quality index detection on to-be-evaluated wheat sprayed with mesosulfuron-methyl, and comparing a detection result with a non-pesticide-applied control or a known variety; and comprehensively evaluating the tolerance of the wheat to mesosulfuron-methyl. According to the invention, a set of multi-index comprehensive evaluation system is constructed. The method effectively overcomes the defects that a traditional method depends on subjective field observation, evaluation lags behind and indexes are single, achieves objective identification of mesosulfuron-methyl resistance of wheat varieties, and provides reliable technical support for rapid screening of resistant varieties, early evaluation of breeding materials and safe field medication. The invention also provides application of the method in preparation of a detection kit.

The application belongs to the technical fields of plant molecular biology and genetic engineering, and relates to a poplar PtoNAC43 transcription factor gene and application thereof. The nucleotide sequence of the gene is shown as SEQ ID NO:1, and the gene encodes a transcription factor protein with a NAC domain. A transgenic plant is obtained by constructing an overexpression vector and introducing the vector into a poplar. The results show that overexpression of the gene can reduce plant height, biomass and growth potential, and affect photosynthesis parameters, endogenous hormone levels and tissue structure. The application can be used for plant growth regulation and dwarf plant cultivation.

The invention discloses a microbial composition with a flowering promoting effect and application of the microbial composition in regulation and control of the flowering time of corn, and belongs to the technical field of agricultural microorganisms. The microbial composition comprises Ensifer strain S281 and bacillus subtilis; a method for promoting precocious flowering of corn comprises the following steps: culturing S281 in an enrichment culture medium to prepare a microbial inoculum; and mixing with a bacillus subtilis inoculant according to a volume ratio of 1: 1, inoculating the mixture into the rhizosphere soil of the corn, and maintaining the corn until the corn blooms. The microbial composition disclosed by the invention can enhance rhizosphere nitrification and regulate endogenous hormone level through the Ensifer strain S281, and can generate a synergistic effect with bacillus subtilis, so that crops such as corn and the like can be obviously promoted to head in advance, and the flowering time can be 2-7 days earlier than that of an untreated control group; and a new microbial solution is provided for realizing green and accurate regulation and control of the growth period of crops.

The invention belongs to the technical field of molecular genetics, and particularly relates to an application of a ZmNAL4 gene or an encoded protein thereof in regulation and control of leaf morphology and plant height of a plant. The analysis of the expression mode of the flavin monooxygenase gene ZmNAL4 in amphiphilic and transgenic plants shows that the leaf width of an overexpression strain is increased by 13.2% and the plant height is increased by 14.8% compared with that of a gene editing strain, and the leaf area and the plant height of an arabidopsis overexpression plant are obviously higher than those of a wild plant, so that the ZmNAL4 has a positive regulation effect on the leaf width; interaction protein analysis identifies that density-tolerant response factors Zmfdx1 and Zmfdx2 and leaf included angle candidate genes ZmPDHE1 and ZmNAL4 interact with each other; plant endogenous hormone analysis shows that the ZmNAL4 regulates and controls the leaf form and the plant height by influencing an auxin signal and a brassinolide path; gene resources and theoretical basis are provided for research on density-tolerant plant types with reasonable leaf widths of corn, and the method has important significance on density-tolerant high-yield breeding.

The present application belongs to the technical field of fruit tree growth regulation, and relates to a regulation method for promoting branch efficiency of fruit trees, and specifically comprises the following steps: step one, limited root cultivation, optimization of root system configuration and function, laying the material foundation for the whole tree nutrient storage and absorption capacity; step two, precise induction, systematic regulation of endogenous hormone balance and metabolism when the root system is in the best physiological state, and directional activation of lateral bud meristem; step three, incision blockage, physical blockage to inhibit signal flow, and at the same time, guiding the enrichment of nutrients to the target bud site, forming a synergistic effect with the chemical induction of step two; step four, spectrum consolidation, providing precise light signals and energy support during the most critical window period of the differentiation and growth of the new lateral bud, maximizing the results of the previous induction. The present application constructs a four-in-one regulation chain of "root-bud-flow-light", significantly improves the branch efficiency, and lays a solid foundation for building a high-yield and high-quality tree crown.

This invention discloses a highly efficient suspension culture method for Rhododendron simsii cells, belonging to the field of plant cell culture and natural product production technology. This method uses TDZ (thiazuron) instead of traditional 6-BA (6-benzylaminopurine) as a cytokinin, combined with four-stage hormone gradient regulation, LC-MS / MS (liquid chromatography-tandem mass spectrometry) dynamic monitoring of endogenous hormones, and staged suspension culture technology to establish a stable Rhododendron simsii suspension cell line. Compared with existing technologies, this method increases callus induction rate from 60% to 90%, reduces browning rate from 22% to 3%, increases cell proliferation from 2.85 times to 4.25 times, increases flavonoid content from 20.8 mg / g to 38.6 mg / g, increases purity from 72.3% to 93.5%, and reduces resource consumption by 97.8%, achieving efficient, green, and sustainable production of Rhododendron simsii flavonoids.

This invention discloses a peony PsPYL4 Genes and their application in regulating root development PsPYL4 The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. This invention was cloned from the peony cultivar 'Zhengwu'. PsPYL4 The gene sequence was obtained and its expression was analyzed in peony. Subsequently, an overexpression vector was constructed and introduced into the target plant to verify its function. It was found that overexpression... PsPYL4 Compared to wild-type Arabidopsis seedlings, the genetically modified Arabidopsis seedlings... PsPYL4 The overexpression lines showed faster growth, larger rosette leaves, significantly shorter taproot length, and significantly increased lateral root number. The levels of endogenous hormones IAA, ABA, ZR, and GAs in the leaves were increased. The average number of roots in the detached leaves was significantly increased on MS medium containing IAA, and the average root length was significantly increased on MS medium containing IBA. It is evident that this gene will have wide applications in the cultivation and genetic improvement of peonies and other horticultural plants.

This invention discloses a peony PsGH3.1 The application of genes in regulating root development PsGH3.1 The nucleotide sequence of the gene is shown in SEQ ID NO.1, and the amino acid sequence of its expressed protein is shown in SEQ ID NO.2. This invention was cloned from the peony cultivar 'Zhengwu'. PsGH3.1 The gene sequence was obtained and its expression was analyzed in peony. Subsequently, an overexpression vector was constructed and introduced into a model plant to verify its function. It was found that overexpression... PsGH3.1 Compared with wild-type Arabidopsis seedlings, the Arabidopsis seedlings with this gene exhibited stunted growth, wrinkled leaves, and shorter pods. Simultaneously, the taproot length was significantly shortened, while the number of lateral roots increased significantly. The levels of endogenous hormones IAA, ABA, and ZR in the leaves increased, while GAs showed no significant change. The detached leaves showed a significant increase in the average number of roots on MS medium containing IAA and a significant increase in the average root length on MS medium containing IBA. This indicates that this gene will have wide applications in the cultivation and genetic improvement of peonies and other horticultural plants.