150 results about "Endogenous hormone" patented technology

The invention relates to the technical field of fruit growing and in particular relates to a growth regulator for improving the fruit setting rate of peaches and a regulating method. The growth regulator comprises the following components of gibberellin and auxin, wherein the auxin may be one of 1-Naphthalene acetic acid, sodium alpha-naphthyl acetate, indoleacetic acid, phenylacetic acid, indolebutyric acid, indoleacetic acid and 2,4-dichlorphenoxyacetic acid, and the growth regulator is sprayed after the peaches bloom. The growth regulator provided by the invention is easy to obtain and prepare and low in economic cost, and can effectively remedy deficiency of endogenous hormone caused by poor fertilization of peaches; the method for applying the growth regulator provided by the invention is simple and convenient and easy to operate and control, effectively solves the problem that in the growing of peaches, the fruit setting rate is influenced by abnormal weather in a flowering phase, shriveled flower buds, overly rapid warming and the like, improves the fruit setting rate by more than 34 times, and is obvious in effect and applicable to popularization and application in the production of peaches in common peach orchards, green houses and other facilities.

The invention discloses a foliage spraying preparation for promoting fruit setting and fruit swelling and enhancing crop stress resistance. The preparation comprises the ingredients including fulvic acid, DA-6, 2,4-D sodium salt, alpha-pimacol, gibberellin, zeatin, dimethyl sulfoxide, butylcellosolve, sodium tetraborate, zinc sulfate, ferrous sulfate, molybdenum sulfate and magnesium sulfate. After the preparation is sprayed, nutrient substances are rapidly transferred from blade surfaces and are gathered at the plant reproduction active part, and the center endogenous hormones level of fruits is improved; the cell division is promoted, the cell size is amplified, the fruit swelling is accelerated, the chloroplast development and the chlorophyll formation are promoted, the photosynthetic efficiency of leaves is improved, and the unit area yield is increased; the flowing of nutrient substances to female flowers is enhanced, the fruit development speed is accelerated, the uniform fruit development is controlled, the deformity is reduced, the fruit surface is kept fresh, bright and smooth, and the commodity rate is obviously improved; and the immunological competence of plants are excited, the resistance is improved, the occurrence of the pest and disease damage is reduced, the senescence is delayed, and the picking period and the fruit shelf period are prolonged.

The invention provides a method for testing plant endogenous hormones, comprises the following steps of: carrying out microdialysis sampling on a plant body; carrying out sample injection on the acquired dialyzate on a high-performance liquid chromatograph; and simultaneously separating and determining four main plant endogenous hormones: gibberellic acid (GA), cytokinin (6-BA), indolylacetic acid (IAA) and abscisic acid (ABA). Compared with the traditional method for testing the plant endogenous hormones, the invention realizes the intravital test on the plant endogenous hormones and can reduce the damage to the plant body to maximum extent; in addition, the invention can simply and conveniently extract samples, has simple operation, can really reflect the change of the content of the endogenous hormones in the plant body in the vital movement progress and realize the real-time, intravital and on-line test on the plant endogenous hormones.

The invention discloses a method for detecting a pear endogenous hormone by using an ultra-high performance liquid chromatography electrospray tandem mass spectrum. The method comprises the following steps of: crushing obtained pear tissues, then adding an extracting solution for extraction, after ending extraction, adding an extraction reagent to a system, and extracting to obtain a crude extract of the endogenous hormone; separating and purifying the crude extract by using a solid-phase extraction column to obtain a detection sample; analyzing a detection sample by using the ultra-high performance liquid chromatography electrospray tandem mass spectrum to obtain the variety and the content of the endogenous hormone in the pear tissues, wherein the extracting solution comprises isopropanol, water and hydrochloric acid, and the volume ratio of the isopropanol, the water and the hydrochloric acid is (1.5-2.5):1:(0.001-0.002), and the extraction agent is dichloromethane. The method has the advantages of high sensitivity, simple pretreatment, high recovery rate, high degree of accuracy and degree of precision and the like and is used for detecting and confirming the endogenous hormones in multiple plant issues.

The invention belongs to a fruit tree yellowing disease preventing and treating comprehensive preparation in fruit tree fertilizers. The preparation comprises (1) a foliage spray micro element fertilizer comprising the following components in percentage by weight: 18-22% of sequestration iron, 13-17% of magnesium, 13-17% of boron, 18-22% of zinc, 9-11% of silicon, 9-11% of selenium, 9-11% of kalium, 0.02% of endogenous hormone and 0.02% of gibberellin, total being 100%, and adding 500-700 times of water for foliage spray; (2) a root-irrigation conditioner comprising the following components in percentage by weight: 11-14% of sequestration iron, 45-55% of citric acid, 2-3% of soil conditioner, 30-40% of nitrogen, phosphorus and potash fertilizers which are same in amount, and trace of protease, total being 100%; and adding 40-60 times of water for the root-irrigation; and (3) a stem-coating nutritional agent comprising the following components in percentage by weight: 18-22% of sequestration iron, 0.1-0.3% of humic acid, 0.1-0.3% of fulvic acid, trace of aspartic acid and threonine and the balance of soft water penetrating agent, total being 100%; and adding 10-20 times of water for stem-coating. After kiwi fruit flowers, before a fruit expanding period (the first-ten and middle ten days of June), the foliage spray micro element fertilizer (the sequestration iron and multiple micro element nutrient solution) can be sprayed on leaves, meanwhile the conditioner (multiple nutrients and enzyme compounds and endogenous root-generating healing agents) is performed. A leaf fast green comprehensive preventing and treating preparation is coated on stems for the serious fruits. Triple effect is united as one, and the purpose of curing the yellowing disease is achieved.

The invention discloses a method for measuring the content of endogenous hormones of burgeons of a tea tree on the basis of high performance liquid chromatography (HPLC). Conditions such as composition, concentration and flow speed of a flowing phase and the adding proportion and the detection wavelength of organic modifying agents are optimized, an optimal chromatographic condition of the content, which is measured by the HPLC, of the endogenous hormones of the burgeons of the tea tree is determined, and repeatability and precision of the method are inspected. The method has characteristics of high speed, high efficiency, high sensitivity and high automation; a tea tree terminal bud sample preparation method and selection and chromatographic conditions of the flowing phase are explored when the hormones of terminal buds of the tea tree are measured by the high performance liquid chromatography, and the method for measuring plant hormones by using the HPLC successfully is reliable and speedy.

The invention discloses a multicomponent composite fertilizer special for peanuts, which belongs to a crop fertilizer. The multicomponent composite fertilizer special for peanuts comprises the following components: 35 percent of total nutrients including 16 percent of nitrogen, 10 percent of phosphorus and 9 percent potassium, more than or equal to 10 percent of medium and trace elements, 7-9 percent of organic matter and 2-5 percent of amino acid. The medium and trace elements comprise trace elements including molybdenum, zinc, manganese, boron and iron. The multicomponent composite fertilizer special for peanuts has the advantages of nutrient content assurance and reasonable proportioning balance. The multicomponent composite fertilizer special for peanuts can effectively improve the immunological competence of the peanuts, regulate endogenous hormone, inhibit the regeneration of insect eggs, activate the activity of root azotobacter, promote root development, quicken nutrient absorption, achieve the functions of strengthening seedlings, promoting growth and promoting flowering, prevent bacterial wilt, continuous cropping symptoms and leaf spots, also prevent excessive growth and take special effect on symptoms including mosaic disease, chlorotic disorder, flocculi, defoliation and the like. After applied, the multicomponent composite fertilizer special for peanuts can effectively improve the percentage of fertile fruits and the thousand grain weight, promote the mellow and full degrees of fruits and achieve remarkable effect of production increase.

The invention discloses an efficient turfgrass endogenous hormone separation and determination method, which relates to the field of the determination of plant endogenous hormones. The method comprises the steps as follows: (1) an extraction liquid is added to perform leaching, and the endogenous hormone is extracted via centrifugation, filtration, decoloration and vacuum concentration; (2) the endogenous hormone is dissolved to obtain a solution, the solution passes through a 96-hole solid-phase extraction small column to be gradually eluted, an eluant is subjected to vacuum concentration so as to obtain a solid-phase extraction eluant; (3) the solid-phase extraction eluant is dissolved with a flux phase, and the obtained flux-phase solution is filtered and loaded on an efficient liquid-phase chromatogram-mass spectra to perform sample analysis. The method is high in detection sensitivity and good in selectivity, needless in derivation, and can directly detect the endogenous hormone of a plant in a crude extraction liquid of a plant sample; a separation and extraction way, chromatogram separation conditions and a mass spectra detection way of the method are suitable for the determination of the turfgrass endogenous hormone, and the recovery rate is high and can reach 92-96 percent; the method can quickly and accurately analyze the contents of the endogenous hormone of four kinds of turfgrass endogenous hormones simultaneously.

The invention discloses an ultra-high performance liquid chromatography-tandem mass spectrometry method (UPLC-MS / MS) for detecting endogenous hormone in tubers of Chinese yams. The method comprises the steps of carrying out dehydration and freeze-drying on fresh samples of the tubers of the Chinese yams by a freezing dryer, crushing and then feeding a solvent for extracting to obtain crude extract liquid; concentrating and drying the obtained crude extract liquid, redissolving the crude extract liquid with phosphate buffer solution, freezing at the temperature of -40 DEG C, unfreezing and then centrifuging to remove impurities; carrying out ethyl acetate extraction for three times, and collecting the ester phase; adjusting the pH of water phase to 2.8-3.5, carrying out ethyl acetate extraction again for three times, collecting and merging the ester phase; concentrating, drying by distillation, and then redissolving the product with methyl alcohol to obtain the detected sample; analyzing the detected sample by the UPLC-MS / MS. According to the method, the pretreatment process is rapid and simple, the hormone extraction and recovery rate is high, and the UPLC-MS / MS is low in limit of detection, high in accuracy and good in linear relation; furthermore, the method is capable of effectively extracting and quantitatively detecting seven hormones in five types of hormones with little content in the tubers of the Chinese yams.

This invention relates to the enhancement of feed efficiency and growth rate, and the reduction of fit accumulation to produce better quality meat by administration of an exogenous gene sequence comprising the complementary DNA sequence of growth hormone releasing factor to stimulate the production of the endogenous hormone peptide growth hormone. This invention further relates to methods for producing such gene sequence.

The invention discloses a method for synchronously separating and measuring an endogenous abscisic acid, gibberellins and auxin in turfgrass, and relates to the technical field of plant endogenous hormone analysis. The method provided by the invention comprises the steps as follows: (1) fast extracting the three major hormones namely, the endogenous abscisic acid, the gibberellins and the auxin; (2) solid phase extracting and eluting; (3) conducting high efficient liquid chromatogram-mass spectrometry. According to the method provided by the invention, the pretreatment is easy and consumes less time, the consumption of a chemical reagent is little, the cost is saved effectively, the detection is high in sensitivity, good in selectivity and requires no derivatization, and as a separation extraction method, a chromatograph separation condition and a mass spectrometric detection method enable such three major hormones as the endogenous abscisic acid, the gibberellins and the auxin to be measured directly and synchronously in the crude extract of a turfgrass sample, the method has a high recovery rate reaching 94.6-96.5%.

The invention discloses a medium and a method for tissue culture and rapid propagation of kiwifruits. The medium for tissue culture and rapid propagation of kiwi fruits comprises a leaf induced differentiation callus culture medium, stem tip or axillary bud inducing germination growth medium, a callus induced differentiation cluster bud medium, a cluster bud proliferation medium and a sugar-free tissue culture induction adventitious root medium. An endogenous hormone rooting technology is adopted, a sugar-free nutrient solution is added into a specific culture container, and the micro-environment is manually controlled, so the rooting and seedling-hardening cycle is shortened, and the transplanting survival rate is improved. Obtained seedlings grow vigorously, have a large root system, andcan be directly transplanted into a nutrition pot or field without being hardened. The production efficiency of a sugar-free rooting technology is 18 times or more that of conventional sugar rootingtechnologies, so the production efficiency of the large-scale production of virus-free kiwifruit seedling is greatly improved.

The invention provides culture media for promoting induction and rooting of cluster buds of dendrobium candidum and a culture method by using rare earth elements. The induction culture medium is prepared by adding a certain amount of Huabao #2, samarium nitrate, banana juice, potato juice, active carbon, agar and white granulated sugar into a 1 / 2 MS culture medium; the rooting culture medium is prepared by adding a certain amount of Huabao #2, europium nitrate, banana juice, potato juice, active carbon, agar and white granulated sugar into the 1 / 2 MS culture medium; According to the culture media and culture method provided by the invention, a proper amount of rare earth elements is added into the culture media, and the proper amount of rare earth elements can increase the content of endogenous hormones of plants, so that the activity of various enzymes is enhanced, the respiratory action of the plants is improved, and H2O2 in tissues is effectively eliminated, and therefore, biomass increase of tissue culture seedlings is promoted, and more tissue culture seedlings with thick roots are obtained. The method has the advantages of being simple and feasible, short in rooting time, developed in root system and economical and practical, and more plants can be obtained, so that the tissue culture seedling survival rate of transplanting can be greatly improved, and the requirement on intensive production is satisfied.

The present invention relates to pesticides field, disclosing a seedling strengthen agent for promoting growth of plant seedling, including dexon, tachigaren, metalaxyl, paclobutrazol, uniconazole, fibrin, humic acidic ammonium, ammonium sulphate, azophoska or mixture of the three for sterilization, increasing seedling content of nucleic acid, protein and chlorophyll, changing endogenous hormones in seedling, increasing seedling resistance, improving edaphic pH value, increasing fertility and promoting growth of plant seedling in angles of sterilization, increasing fertility, etc.

The invention discloses a determination method of the content of endogenous hormones in terminal buds of tomato plants. The method comprises the following steps: 1) preprocessing a tomato terminal bud sample for test; 2) preparing a mobile phase and setting chromatographic conditions; 3) preparing a standard liquid; 4) inspecting specificity; 5) inspecting a linear relation; and 6) determining sample content. The method of the step 1) comprises process of performing vacuum evaporation after extraction and petroleum ether extraction, further regulating pH, carrying out ethyl acetate extraction, and finally performing secondary vacuum evaporation to obtain a to-be-determined sample liquid; and in the step 2), the mobile phase is prepared from methanol and 0.075% glacial acetic acid. The determination method of the content of endogenous hormones in terminal buds of tomatoes by HPLC, provided by the invention, can quickly, accurately and simultaneously determine the content of four plant endogenous hormones, and has the characteristics of fast and efficient determination, high sensitivity and high degree of automation; a recovery of standard addition experiment is carried out, the average recovery is 99.85%, and RSD (Relative Standard Deviation) is less than 1.56%, which meets the hormone determination requirement.

The invention provides a sample pre-treatment method for determining an endogenesis hormone content of towel gourd seeds by adopting an HPLC (High Performance Liquid Chromatography), comprising the following treating steps of: preparing a towel gourd seed plant hormone rough extraction solution to be detected; concentrating the rough extraction solution and adding 3 ml of phosphoric acid buffering solution with the pH (Potential of Hydrogen) of 8.0 and the concentration of 0.1 mol / L; freezing and degreasing; filtering and removing impurities; adjusting the pH value to 3.0-3.5 by using diluted hydrochloric acid with the concentration of 0.5 mol / L and utilizing ethyl acetate with the same volume to extract for three times; mixing ester phrases; concentrating the ester phases and utilizing a flow phase to adjust the volume to 1 ml; and filtering with a micro-pore filtering film with the size of 0.45 microns and utilizing the HPLC to analyze. The method disclosed by the invention has the advantages of simplicity in operation and low cost; the method can treat a plurality of samples one time so that the time can be saved, column treatment is not carried out in a purification process, and loss of objects to be detected in an extraction process is reduced. The method disclosed by the invention can be used for pre-treatment before extracting a plurality of types of plant hormones and can meet the requirements of treating samples by adopting the high performance liquid chromatography.

The invention relates to a method for regulating the seedling tree stage transformation of peach trees, which continuously measures and analyzes the contents of ABA, GA, iP and ZT in the endogenous hormones of different node samples, so as to determine the starting and finishing critical points of seedling tree stage transformation. Against the critical points, regulation measures can be used to the seedling trees to inhibit the nutrition growth, lower the inflorescence nodes for earlier blooming and fruiting, thus reducing the breeding cycle. In the very year after the seeding of peach seedling tree, the juvenescent phase is passed, while the stage transformation regulation is carried out in the second year; the peach trees bloom and fruit in the third year, and the initial selection of hybrid is finished in the fifth year, which is one years ahead of the time with the conventional technology and save 16.7 percent of breeding cost.

The invention discloses an improved method for locating endogenous hormone IAA in bamboo plant tissue, ans aims to improve the existing immunogold-silver staining method. TBS is used as a buffer solution and solvent in the experiments to substitute PBS, TBS does not generate precipitate with calcium ions, and has extremely low interference on biological reaction, thus reduce some nonspecific coloration background interference and improve the accuracy of the results; by adding gelatin in the color liquid, chromogenic time is basically controlled in 15-20 min, and time is enough to check the chromogenic level in the chromogenic period, so as to obtain good repetition rate; and incubation of secondary antibody at room temperature shortens the reaction time. The method of the invention not only streamlines reaction steps, and has mild reaction conditions and easy control of coloration, can obtain more elegant and accurate image, and has good application and popularization value in the immune localization of bamboo plant endogenous hormone.

The present invention provides diagnostic in vitro methods as well as kits and devices to be used in the methods of the present invention for diagnosis or prognosis of a pathologic condition characterized by the presence / absence of an endogenous hormone and / or hormone analog(s) thereof involved in diabetes or metabolic syndrome. The methods comprise a quantitative separation of at least those analytes of interest whose common presence interferes with measuring the presence / absence or concentration of one of the analytes of interest by a subsequent analytical method.

A Chinese patent medicine for improving the function of female ovary is prepared from wolfberry fruit, safflower and rehmannia root through respectively drying and pulverizing by 20 meshes, proportionally mixing in macropolar extracting liquid, filtering for removing dregs, drying, pulverizing and shaping.

The present invention relates to a Chinese medicine for curing puerperal subinvolution of uterus and its preparation method. It is made up by using 10 Chinese medicinal materials of cuscuta seed, lycium berry, glehnia root, Chinese angelica root, white peony root and others as raw material through a certain preparation process. Said invention Chinese medicine has the advantages of improving subinvolution of uterus, promoting contraction of uterine smooth muscle, raising endogenous hormone level, resisting inflammation, stopping pain, arresting hemorrhage and accelerating involution of uterus,etc.

The invention belongs to the technical field of seedling cultivation, and particularly relates to a seedling method of five-pointed star loquat. The method comprises the following steps of female parent processing, substance cuttage, cuttage and daily management; according to the seedling method, a scientific and reasonable seedling management mode is formed, rooting of cutting slips can be effectively promoted, the method is beneficial for the growth and development of the root system, and the growth of fibrous roots of the cutting slips is promoted; the cutting slips absorb nutrients in the rooting process, the content of endogenous hormones of plants and the activity of important enzymes are adjusted, the metabolism intensity of the plants is adjusted, the survival rate is high, the diseases and insect pests are few, and the growth condition of the root system is good.

The invention discloses a method for improving the plumpness of direct-sowing rice grains through exogenous hormones and a growth regulator. The exogenous hormones ABA and Spm and monopotassium phosphate of the plant growth regulator are applied to the direct-sowing rice directly to improve the plumpness of the direct-sowing rice grains. The ABA or the Spm is sprayed in the early period of grouting of the direct-sowing rice, namely 9 days after flowering, and accordingly the corresponding endogenous hormone level can be significantly improved, the plumpness of the grains can be promoted, and the maturing rate, the plumpness of the grains and the thousand seed weight can be significantly improved, wherein the concentration of the ABA is 25 x 10-6 M and the concentration of the Spm is 5 x 10-4 M. The 500 g per mu of monopotassium phosphate is sprayed in the poured 3 leaf stage of the direct-sowing rice and accordingly the maturing rate, the plumpness and the thousand seed weight can be significantly improved and the yield can be improved by more than 10%. Accordingly, the plumpness of the rice grains can be significantly improved due to the fact that the suitable exogenous hormones and growth regulator are utilized in the suitable using period, the risk of the grouting of the direct-sowing rice grains can be avoided, the yield of the direct-sowing rice can be improved, the rice quality can be improved, and the production development of the direct-sowing rice can be promoted.

The invention relates to the field of seed science, especially to a corn coleorhiza endogenous hormone detection technology based on a GC-MS internal standard method. The technology comprises processes of sample acquisition work, sample extraction work, purification and enrichment work, GC-MS / SIM detection work, post-processing and the like. A coleorhiza tissue sample is acquired by a horizontal and vertical stripping method; 80% methanol (cold) is used for extraction, and endogenous hormone is extracted through eight steps; by the use of a Sep-Pak C18 column, sample purification and enrichment including nine steps are carried out; hormone is detected by the use of GC-MS Agilent DB-17ms; and hormone content is obtained through post-processing such as optimization of detection conditions and the like. Contents of eight endogenous hormones of corn coleorhiza are detected based on the GC-MS internal standard method, and the detection effect is enhanced by the technical combination of ''horizontal and vertical stripping+ extraction+ solid-phase extraction+ Agilent DB-17ms''. The technology can be applied in researches on the mechanism of corn seed germination.

The invention relates to the technical field of biology, in particular to application of melatonin serving as an effective component of a foliar spraying agent to influence on rhizosphere soil microorganism diversity of medicinal plants. The content of the melatonin in the foliar spraying agent is 100-300 mg / L, and an inducer further contains 0.05 g / L of hydroxypropyl methyl cellulose. The melatonin can reduce the content of endogenous salicylic acid and hydrogen peroxide of plants, increase the content of jasmonic acid, promote proliferation and endosymbiosis of rhizosphere probiotic fungi and change the rhizosphere soil microorganism diversity of the paris polyphylla. In conclusion, when the melatonin is sprayed on the leaf surfaces, the composition of rhizosphere microorganisms can be adjusted by changing the endogenous hormone level, so that the effects of resisting diseases and promoting growth of the paris polyphylla are achieved. The foliar spraying agent taking the melatonin as the effective component is used for cultivating paris polyphylla var chinensis and has the effects of resisting diseases and promoting growth, the preparation and use methods are simple, the cost is low, and popularization and application can be realized.

The invention discloses an application method for promoting female flower differentiation of litchi trees. In the amphiprotic period of litchi flowers, the application method includes dripping ethephon solution to the litchi trees via a liquid hanging method. Ethylene is endogenous hormone of plants and has the functions of promoting female flower differentiation, suppressing male flower development, controlling treetop growth, reducing nutrient translocation and waste and increasing yield. The ethylene is artificially synthesized chemical modifier and can be absorbed by the plants in vivo to be released in functioning. Therefore, via dripping the ethephone solution to the litchi trees via the liquid hanging method, female flower differentiation of the litchi trees is effectively adjusted, and an important reference mode is provided for production development of the litchi.

The invention discloses a method for prolonging the flowering phase of North American begonia, and belongs to the technical field of planting. The method includes the steps: (1) seedling selection; (2) seedling modified cultivation; (3) seedling reinforced cultivation. One-age North American begonia seedlings are specially cultivated, growth characteristics of the seedlings are improved by co-treatment of rich water, illumination, temperature and ultrasonic, nutrient absorption characteristics and endogenous hormone secreting characteristics of the seedlings in the flowering phase starting in the middle of April are enhanced, the seedlings are more thoroughly modified under ultrasonic induction and secondary reinforced cultivation, long flowering phase can be continuously kept at a subsequent growth stage, existing annual flowering phase prolonging operation is changed, the flowering phase is fundamentally prolonged, and popularization and use values are high.