3 results about "Cuscuta" patented technology

The invention relates to the technical field of traditional Chinese medicine, and discloses a plant combined extracting solution for treating protrusion and bulging of intervertebral disc and a preparation method thereof, the plant combined extracting solution comprises plant extract powder and medicinal liquor, and the plant combined extracting solution, tincture and paste disclosed by the invention. The medicine comprises radix trichosanthis, cortex phellodendri, rheum officinale, turmeric, radix angelicae, purple officinal magnolia bark, Guangdong pericarpium citri reticulatae, liquorice, rhizoma atractylodis, erycibe obtusifolia benth, cinnamon, angelica sinensis, radix scrophulariae, radix paeoniae rubra, radix rehmanniae, semen momordicae, sophora japonica twig, willow branch, frankincense, myrrh, ephedra, notopterygium root, ligusticum wallichii, rhizoma cyperi, elecampane, fructus aurantii, rhizoma atractylodis macrocephalae, semen cuscutae, fennel, semen armeniacae amarae, rhizoma alismatis, fructus gleditsiae, moutan bark, fructus psoraleae, rhizoma polygonati and Chinese yam. 36 plants are compounded, a liquor agent, a tincture, a fluid extract agent and an extract agent can be obtained by adopting an extraction method, and a certain agent extracted from the plants is directly externally applied to a part during treatment, so that a brand-new effective external application treatment scheme for treating the protrusion and bulge of the intervertebral disc and complications of the protrusion and bulge of the intervertebral disc is provided.

This invention discloses a TaqMan real-time fluorescent PCR detection primer and probe set, kit, and detection method for Cuscuta pentaphyllum. The primer and probe set includes: an upstream primer C. pan F, whose nucleotide sequence is shown in SEQ ID NO.1; a downstream primer C. pan R, whose nucleotide sequence is shown in SEQ ID NO.2; and a TaqMan probe C. pan P, whose nucleotide sequence is shown in SEQ ID NO.3. The primers and probe target specific fragments of the rbcL gene sequence of Cuscuta pentaphyllum, achieving specific recognition of Cuscuta pentaphyllum through sequence complementarity matching. This avoids false positive results caused by cross-reactions with closely related species and host plants at the molecular targeting level, effectively solving the problem of difficulty in distinguishing closely related species in traditional morphological identification. Compared with traditional morphological identification and conventional PCR, this invention also features high detection efficiency, short processing time, simple operation, and wide applicability. It is suitable for precise identification in professional laboratories and can also meet the practical applications in grassroots quarantine and field monitoring scenarios.

The application belongs to the technical field of medicine, and particularly relates to an extraction method and medical use of a Cuscuta plant resin glycoside extract. 1 The H NMR signal is used as a guide to rapidly locate and enrich resin glycoside parts of six Cuscuta plants, and a main resin glycoside component Cus-1 in Cuscuta is obtained by separation. And through the oligoasthenospermia model mouse experiment, it is confirmed that the six resin glycoside parts and Cus-1 all have good oligoasthenospermia treatment activity, and can be used as active ingredients for preparing medicines for preventing or treating oligoasthenospermia, and have wide application.