116 results about "Daphnia" patented technology

A methodology for bioassays and diagnostics in which a particulate label (ranging in size from nm-scale molecular assemblages to organisms on the scale of tens or hundreds of microns), such as, but not limited to, nanoparticles, bacteria, bacteriophage, Daphnia, and magnetic particles, serve carriers for analytes bound by molecular recognition elements such as antibodies, aptamers, etc. The described methodology is generally applicable to most pathogen assays and molecular diagnostics and also leads to enhanced sensitivity and convenience of use.

The invention relates to a comprehensive treatment method of aquiculture sewage comprising the following steps: providing a ecological treatment pool, discharging the aquaculture sewage into the ecological treatment pool, then inoculating photosynthetic bacteria and chlorella to reach a first ecological environment; inoculating rotifer and moina macrocopa to reach a second ecological environment; drying the rotifer and daphnias at a low temperature after collecting the same to obtain the solid products; discharging the filtrate after ozone treatment; supplementing new aquiculture sewage into the ecological treatment pool and repeating the above treatment. The method of the invention constructs an integration technology system about physicochemical, biology, ecological combinative technology and provides feasible technical support for the sustainable development of the intensive breeding industry and the establishment of the circular economy mode.

The invention relates to a method for constructing a specific pathogen-free penaeid shrimp culture system, which comprises the steps of adopting comprehensive treatment of culture water for avoiding the entry of pathogens; introducing selected oocystis borgei into a penaeid shrimp culture pond for constructing a good microalgae community structure, and using a microalgae-controlled water environment to reduce the concentration of injurious factors, such as ammonia nitrogen, nitrite nitrogen and the like in a water body, improving the water quality, eliminating stress factors, improving the content of dissolved oxygen in the water and leading the environment of the water body to be in the good dynamic balance state for a long time. A system of the oocystis borgei, rhodopseudanonas palustris and phycomycete is utilized for inhibiting the growth of vibrios during the early stage, the middle stage and the late stage of culture; and schmackeria dubin is utilized to intake microalgae, thereby controlling the number of the microalgae in the water body and eliminating the adverse impacts of rapid proliferation of the microalgae on a culture environment. Penaeid shrimps have the advantages of specific pathogen-free property, rapid growth, strong disease resistance, strong stress resistance, short culture period, large and uniform formed size, high output, good investment efficiency and the like.

The invention discloses a kiwi planting method applicable to low-lying and flat areas, which belongs to the technical field of plantation of kiwis. The kiwi planting method is implemented by the parts including a kiwi planting field, kiwi trees, tree shed frames, a sprinkling irrigation system, a goose dung fermentation room, drainage ditches, goose flocks, goose houses and rye grass, wherein the kiwi planting field is larger than 0.5 mu; the more than 100 kiwi trees are planted in rows; the sprinkling irrigation system is buried underground; the goose dung fermentation room is built beside the kiwi planting field; the drainage ditches are excavated on the kiwi planting field in a vertical and horizontal intersection manner; the goose houses are arranged among the kiwi trees; and the rye grass is planted on the ground around the kiwi trees and the goose houses. The kiwi planting method has the advantages of comprehensive breeding, ecological cycle and environment friendliness; and excrement of gooses serves as optimal organic fertilizer for the kiwis and the rye grass, the rye grass serves as optimal feed for the goose, and earthworms, chironomus larva, water fleas and weeds in the drainage ditches serve as natural food for loaches.

A method for rearing hybrid salmon seeds is used for solving the problems that presently, death rate of hybrid salmon seeds obtained by mating Brachymystax lenok with taimen is high, and techniques of rearing seeds of other salmon species is highly complex and difficult to industrialize and is unsuitable for rearing hybrid seeds of Brachymystax lenok and taimen. The method includes: placing fertilized eggs of hybrid salmon into sterilized parallel tanks, incubating the fertilized eggs in the shade till fry begin hatching, and removing egg skin, dead eggs and dead fry during hatching; and using water fleas as biological bait for feeding when hybrid salmon fry open mouths, using Tubificidae to feed the fry on the seventh day to the tenth day after mouth opening, mixing Tubificidae with complete feed into muddy feed for feeding, and using the complete feed to feed the fry. Hybrid salmon seeds reared by the method are high in quality, and the survival rate can be increased to more than 80%.

The invention provides a manufacturing method of a daphnia microslide specimen, which comprises the following steps: firstly fixing the daphnia with a fixative, simple-staining or double-staining, color separating, and stepwise dehydrating with ethanol; then stepwise displacing the ethanol in the dehydrated daphnia with acetone; and finally sucking out the daphnia which is subject to the displacement with acetone, dripping on a glass slide, and dripping prepolymerized polymethacrylate to seal the glass slide to obtain a daphnia microslide specimen. The daphnia microslide specimen manufacturedby the method enables the cell borderlines of the daphnia to be obvious, the intestinal tract, ovary and other structures to be observed more clearly, the transparency is increased, and the drying time is shortened.

The invention provides a method for inducing copepoda to produce diapause eggs and relates to aquaculture. The method comprises the steps that an algae solution is cultured; seawater is treated; two-generation taming culture is performed on acartia pacifica first, eggs laid by the tamed acartia pacifica are hatched to obtain nauplii, and the nauplii with high vitality are selected under an anatomical lens for use; induction conditions are set; the diapause eggs are screened and collected, wherein each group of 100 nauplii is placed in a 1L crystallizing dish, a test is performed according to the induction conditions in the fourth step, water is changed every other day for each group, when the acartia pacifica start laying eggs, 48-micron bolting silk is used for collecting the eggs every 24h for five days in total, the total number of the laid eggs is recorded, the eggs collected every day are observed under the anatomical lens, the eggs with rough and uneven adventitia and spiny protrusions are the diapause eggs, the solid diapause eggs with a complete form are screened out, and the number of the diapause eggs is recorded.

The invention discloses a special bait for fishing crucian. The special bait comprises the following raw materials in parts by weight: 20 to 50 parts of earthworm powder, 30 to 50 parts of blood worm powder, 20 to 40 parts of river shrimp powder, 20 to 50 parts of euphausiid shrimp powder, 10 to 30 parts of cyclop powder, 5 to 20 parts of scallop meat powder, 8 to 15 parts of field snail powder, 10 to 30 parts of oat flour, 10 to 25 parts of soybean meal, 4 to 10 parts of sweet potato powder, 5 to 20 parts of potato powder, 5 to 18 parts of wheat protein flour, 3 to 8 parts of mountain flour, 5 to 15 parts of clove, 0.5 to 1.5 parts of corn essence, 1 to 3 parts of maltose, 0.5 to 2 parts of sodium cyclamate, 0.4 to 2 parts of honey, 0.2 to 0.7 part of dimethyl-beta-propiothetin, 0.3 to 1 parts of betaine, and 2 to 7 parts of compound vitamins. The special bait for fishing the crucian has the advantages that the nutrients are complete, the price is low, the preparation is easy, the fish collecting speed is high, the usage amount is less, the easiness in dissolving in water is avoided, the fish retention time is long, and the fish luring and fishing effect is better.

The present invention discloses a method for culturing the young Chinese soft-shelled turtles. Said method includes the following steps: for newly incubated young soft-shelled turtles using living water fleas and earthworm to feed them in indoor clear water temporary nursery; and for small young soft-shelled turtles using artificial feed whose coarse protein content is above 3-5% to feed them, firstly feeding them in greenhouse, then culturing them in outdoor pond.

A method for using Rana catesbeiana to control pests in paddy fields includes: fencing a paddy field of certain area; digging drainage ditches on the paddy field, wherein the drainage ditches lead to an inhabiting pond for Rana catesbeiana, and the inhabiting pond accounts for 1-2% of total area of the paddy field; setting up a supported shed above the inhabiting pond; mounting efficient trap lamps on the supported shed; and putting Rana catesbeiana in the paddy field to breed. Rana catesbeiana belongs to carnivorous animals and take food in a wide range, crop pests taken by Rana catesbeiana account for 82% of total food taken by Rana catesbeiana and include snout moth, grasshopper, mole cricket, water flea, fly larvae and other insects, and accordingly, the pests are controlled effectively, and chemicals for use are reduced. By the method, pollution and destruction of plants and fields by chemical pesticides are avoided, ecological environment and physical health of people are protected, increase of rice yield can be achieved, Rana catesbeiana can be reclaimed from the fields to serve as commodities, income of farmers is increased, and overall development of green and ecological agriculture is promoted.

The invention relates to a method for cultivating larval stage feed copepods for ocean fancy fishes and relates to biological bait cultivation. The method comprises the first step of collecting the copepods, observing the copepods with oocysts, measuring the body lengths of the copepods, performing identification of species, and obtaining new acartia, wherein the mature individuals are 680-740 microns long and belong to calanoida, acartiidae and acartia; the second step of performing inoculated culture of the new acartia in a container, wherein the body-length distribution is that the nauplii are 60-230 microns and copepodite are 310-640 microns, and the nauplii and the copepodite serve as larval stage biological baits of marine fishes; the third step of placing the new acartia obtained through the second step in a water tank to go on being cultivated, feeding baits in the cultivation process, siphoning residual feed excrement each day, collecting part of the nauplii and copepodite to serve as larval stage feed for the ocean fancy fishes, adding the baits, and performing continuous cultivation for 1 month and then changing the water. The cultivation density of the new acartia can reach 2000/L.

The invention relates to the technical field of bait, in particular to a preparation method for bait in dual purposes of fish luring and fishing. The bait is made of raw materials comprising, by weight, 25-30 parts of ripened wheat germ powder, 12-15 parts of wheat grain active gluten powder, 8-10 parts of potato flake powder, 3-4 parts of high-protein flour, 8-10 parts of fried bean flour, 2-3 parts of rape cakes, 2-3 parts of wolffia arrhiza dry powder, 2-3 parts of dried snail meat powder, 1-2 parts of krill powder, 1-2 parts of wild lettuce leaves, 1-2 parts of sunflower pollen, 1-2 parts of tender cynanchum the-sioides, 1-2 parts of water flea powder, a moderate amount of salt and 0.2-0.5 part of feeding promoting agents. The bait can serve as fishing bait and take the place of the bait, and the process of throwing in the fishing bait to attract schools of fishes in a traditional fishing step is simplified, so that fishing cost is saved; the bait smells fragrant and less prone to dissolving in water, plenty of schools of fishes can be attracted, and hooking probability of big fishes is increased.

This invention discloses a joint control algae method of Cladophora and transparent algae involving a water disposal method, which comprises the following steps: preparing open to ambient air net cage with rustless steel silk net of bore diameter at 3-8mm in preformed precipitate pool of water supply works; fixing nylon twine net of cross-paralleling and verticalling with bottom of net cage on the top of net cage; setting bore diameter of nylon twine net at 1-2cm; constructing floating net cage with fixed float of quadrangle of the top of net cage; making side wall of floating net cage exceed waterborne at 0.2-0.4m; residing 3-4 weeks in the water body; growing up Cladophora on the nylon twine net; making transparent algae natural grow in the floating net cage; or leading into floating net cage from the outside; setting density of transparent algae at 10-40 /L; picking Cladophora when Cladophora breed 1-2 weeks on the nylon twine net.

The invention aims at providing a temperature resistance domestication method for daphnia and a method for conducting ecological repair on a water body through daphnia. The method includes the steps that A, daphnia is placed in a breeding solution to be bred for a set period of time, and the temperature of the breeding solution is changed into the preset temperature according to the same tendency; B, the step A is repeatedly executed till the temperature of the breeding solution reaches the preset limiting temperature, beneficial microorganisms are put in, and domestication of daphnia is completed. By putting daphnia domesticated according to the method in the water body rich in toxic substances and algae, the water body can be purified, the underwater ecological balance can be repaired, and the ecological self-purification of the water body can be achieved.

The invention discloses a method for feeding wild catfish and carp living in Yellow River in ecosystem environment. The method comprises the following steps: injecting Yellow River water into common earth, crushing planted fodder grass; adding a composite micro-ecological viable bacteria preparation into the crushed fodder grass; adopting a closed anaerobic fermentation process to prepare a yeast material; scattering the yeast material once every ten days so as to provide nutrient components required by the growth of beneficial alga and plankton unicellular organisms to feed and breed daphnia, algae and float grass, and to feed wild trash fish fries and river prawns; and using Yellow River circulating water and living food to feed the wild catfish and carp. The method uses natural ecological resources to develop natural Yellow River wild fishes for breeding, forms a complete a natural food chain, and maintains natural quality of Yellow River fishes. The method is applied to both coasts of Yellow River valley, utilizes natural resources to develop wild fish ecological feeding in large scale, and provides a novel approach for developing domestic food resources.

The invention discloses a synergetic control-algae method of silver carp, variegated carp, Cladophora and transparent algae involving water disposal method, which comprises the following steps: raising silver carp and variegated carp in eutrophication water body; constructing floating net cage; attached growing Cladophora on the nylon twine web of floating net cage; placing transparent algae in floating net cage. This invention resolve the water quality deteriorate problem because overgrowth of algae. The average clearance of phosphoric is at 70-73% with algae at 73.5-75.5%, oligon at 67.5-70.5%.

The invention discloses a waste water online biological monitoring method and a waste water online biological monitoring device, and belongs to the field of waste water online monitoring. The device consists of a circulating pump (3), a liquid storage chamber (2), a circulation chamber (1), an inductive electrode (4) and a multimeter (5); the circulating pump is arranged at the lateral bottom of the liquid storage chamber away from the circulation chamber, and is connected with the circulation chamber via a plastic pipe; the sidewall of the circulation chamber is connected with side surface of the liquid storage chamber at the equal height to realize flow exposure of the whole course. Two pairs of copper electrodes with nuts are arranged on the inner side of the circulation chamber, wherein one pair of the electrodes functions as an input end of an electrical signal, and the other pair of the electrodes is connected with the multimeter to form an output end of the electrical signal. The distribution of an electric field in the circulation chamber can be influenced by swimming of the tested organism daphnia, namely the change of output current or voltage. By the invention, whether the quality of the discharged water is safe can be intuitively reflected; meanwhile, an 'electronic eye' is provided for the pollution incident monitoring and the dispute resolution monitoring of an environmental monitoring department. The device disclosed by the invention is simple in structure and strong in mobility and can be used indoors and outdoors.

The present invention relates to feed for pseudobagrus ussuriensis fries and advanced fries, and belongs to the field of special compound feed and processing thereof. The special compound feed is good in palatability, balanced in nutrition, easily available in raw materials and pollution-free in aquaculture water body, and can improve the immunity of pseudobagrus ussuriensis fries and advanced fries. The feed is characterized in that the feed consists of the following ingredients in mass percentages: fish meal 30-40%, daphnia powder 20-30%, meat and bone meal 10-15%, full-fat soybean 10-15%, cottonseed meal 2-5%, beer yeast 2-5%, flour 1-7.5%, 1% calcium dihydrogen phosphate 1%, compound enzyme preparation 0.5%, immunopotentiator 0.5%, garlicin 0.01%, choline chloride 0.3%, liver and gallbladder Kang 0.5%, multi-vitamin premix 0.5% and multi-mineral premix 0.5%. The preparation process includes: raw material crushing, mixing, extruding and granulating. The feed is simple in process, balanced in nutrition and easily available in raw materials, and solves the problems of pollution in the aquaculture water body and poor disease resistance of the fish body.

The invention relates to the technical field of aquaculture, and specifically relates to a feed for brocaded carp. The feed is obtained by the following raw materials by weight: 45-50 parts of corn, 10-12 parts of fried chopped peanuts, 18-20 parts of soybean meal, 4-5 parts of tubificidae powder, 12-15 parts of carrots, 2-3 parts of honey, 10-12 parts of red bean powder, 8-10 parts of black bean powder, 4-5 parts of daphnia powder, 4-5 parts of tenebrio moliter powder, 4-5 parts of wolffia arrhiza powder, 10-12 parts of caraway, 3-4 parts of freshwater shrimp meal, 8-10 parts of carrot skins, a proper amount of salt and 4-5 parts of an phagostimulant. The prepared feed for the brocaded carp provides adequate nutrition for growth and development of the brocaded carp and can promote the growth and development of the brocaded carp. Appetite of the brocaded carp is good; utilization rate of the feed is high; the raw materials such as the red bean powder, the freshwater shrimp meal and the carrots can improve color of the brocaded carp, thereby enabling the brocaded carp to be gorgeous and enhancing ornamental values.

The invention discloses a compound feed for promoting young giant salamander growth at a larval stage. The compound feed comprises a first component and a second component, wherein the first component comprises the following raw materials in parts by weight: 20-25 parts of earthworm, 11-14 parts of tadpole, 5-10 parts of freshwater shrimp, 15-20 parts of acete chinensis, 12-15 parts of silkworm chrysalis, 5-8 parts of grub, 15-18 parts of bean worm, 19-22 parts of maggot, 15-20 parts of wiggler, 7-10 parts of water flea, 8-11 parts of loach, 2-4 parts of crucian fries and 2-4 parts of grass carp fries; and the second component comprises the following raw materials in parts by weight: 60-70 parts of fish meal, 10-15 parts of fermentation peanut meal, 5-10 parts of fermentation soya bean meal, 16-19 parts of egg white, 7-9 parts of casein, 7-9 parts of oleum morrhuae, 2-3 parts of vitamin, 2-4 parts of a mineral substance, 2-4 parts of sodium alginate, 0.5-1 part of probiotic, 3-5 parts of medicated leaven, 3-5 parts of astragalus membranaceus, 3-5 parts of poria cocos, 1-3 parts of herba leonuri, 1-3 parts of rhizoma atractylodis macrocephalae, 1-2 parts of ligusticum wallichii, 6-8 parts of hawthorn fruits, 3-4 parts of liquorice, 3-5 parts of radix stemonae, 2-4 parts of radix isatidis and 1-3 parts of cyrtomium fortunei.

This invention relates to a test method for Cyclops kind zooplanktons in water and its larva numbers, which takes a white tray as a test container, applies suitable artificial radiation and adds a certain volume of potassium permanganate solution to mix them by a glass rod and watch it to get the number, which can test numbers of Cyclops kind zooplanktons in water and its larvae quickly and accurately.

The invention discloses a protective agent with dimethyl sulfoxide, maleic acid and sodium hyaluronate, and further discloses a freeze drying and refrigeration method for storing dormant eggs of eurytemora pacifica for a long term. The freeze drying and refrigeration method includes sieving and thoroughly cleaning the dormant eggs and then transferring the dormant eggs into a container with the protective agent; filling the container with circulating nitrogen dried by inert chemical drying agents; transferring the dormant eggs into light-resistant containers after the eggs are dried, and hermetically storing the dormant eggs. The freeze drying and refrigeration method has the advantages that the integrity of matrixes of the dormant eggs of the eurytemora pacifica can be kept by the protective agent, damage to tissue structures of the dormant eggs can be abated, the high activity of the dormant eggs can be kept, and the hatching rate of the dormant eggs can be increased; technologies ofthe freeze drying and refrigeration method for storing the dormant eggs of the eurytemora pacifica for the long term are easy to operate, the freeze drying and refrigeration method is repeatable, safe and reliable and is low in cost, systems are stable and dry, environments can be sterilized, the survival rate of the dormant eggs can be increased, and the purpose of storing large quantities of dormant eggs for a long term can be achieved.

The invention belongs to the technical field of aquaculture, and particularly relates to a macrobrachium rosenbergii feeding method. According to the method, in the macrobrachium rosenbergii breedingprocess, water daphnia is adopted as a feeding bait or one of feeding baits. With the adoption of the special feeding method provided by the invention, the feed consumption can be reduced, a natural growth state is approached, the cultured macrobrachium rosenbergii is larger in size than macrobrachium rosenbergii bred by common breeding, and the weight of each macrobrachium rosenbergii is three times or more of the weight of the macrobrachium rosenbergii bred by common breeding. A special bait and the feeding method are adopted for comprehensively increasing the yield of the macrobrachium rosenbergii; the yield of the macrobrachium rosenbergii is 1000 kg per mu or more; the quality of the macrobrachium rosenbergii is optimized; the surface of the shrimp body is smooth, and the vitality isgood; no peculiar smell exists; the medicine residue inspection is qualified; the size is neat; and the size is completely achieved.