11 results about "Daphnia" patented technology

The invention discloses a daphnia magna cultivation device and system, and relates to the technical field of aquatic organism cultivation, the daphnia magna cultivation device comprises a daphnia magna cultivation box, a first screening plate and a partition plate, the bottom end of one side of the daphnia magna cultivation box is provided with a first outlet, the first outlet is provided with a first valve, and the daphnia magna cultivation box is provided with a first light source close to the first outlet; a second light source is arranged far away from the first outlet, and the first screening plate and the partition plate are detachably mounted in the daphnia culture box; when the device is used, the first outlet is in a closed state, the functions of daphnia magna screening and culture solution replacement can be achieved at the same time by controlling opening and closing of the first light source and the second light source and installing the first screening plate and the partition plate in the daphnia magna culture box, the screening efficiency is improved, a straw is not needed to suck a disturbance object, damage to the disturbance object is reduced, and the practicability is high. The daphnia magna cultivation system can also achieve the functions of feeding and culture solution filtering and recycling.

This invention discloses a test method for evaluating the inhibitory effect of microbial pesticides on the activity of Daphnia macrocarpa, relating to the field of pesticide detection. The method includes an outer casing, a layered test cylinder, an air supply assembly, a water quality monitoring probe, and a light source. The outer casing has a hinged lid and a transparent observation window on its front. The layered test cylinder is housed within the outer casing. This method innovatively uses a layered test cylinder composed of a test cylinder, an isolation cylinder, and a pesticide loading cylinder. The Daphnia macrocarpa in the test cylinder is isolated from the microbial pesticide in the pesticide loading cylinder, but the water in the test cylinder and the pesticide loading cylinder exchange water through an exchange port. This method realistically simulates the natural exposure state of Daphnia macrocarpa in water containing microorganisms and effectively avoids the problem of microbial adhesion interfering with Daphnia macrocarpa activity, thus significantly improving the accuracy of the test results.

The application discloses a method for domesticating freshwater daphnia by using marine bacteria and belongs to the technical field of daphnia breeding. The method comprises the following steps: expanding the captured freshwater daphnia; adding bacillus velezensis freeze-dried powder into freshwater to activate, then adding the bacillus velezensis freeze-dried powder into seawater culture medium to expand, finally centrifuging to collect bacterial bodies and storing the bacterial bodies at low temperature for standby; when the freshwater daphnia is expanded to small individuals, the collected bacillus velezensis bacterial bodies are used as food to feed the freshwater daphnia until the freshwater daphnia adapts to target salinity seawater. The domestication method changes the food source of daphnia, improves the salt tolerance of the freshwater daphnia, and effectively solves the problem of poor domestication result in the prior art.

The invention discloses a method for controlling growth of cylindrotheca. The method comprises the following steps: step 1, capturing daphnia europaea and carrying out single-individual culture; step 2, carrying out toxic domestication culture on the daphnia europaea; step 3, selecting toxic domestication genotypes of the daphnia europaea, and step 4, controlling the growth of the cylindrotheca by utilizing the daphnia europaea with the toxic domestication genotypes. According to the method disclosed by the invention, toxicity domestication is carried out on the daphnia europaea by feeding food to the cylindrospora virulent strain for culture, so that the daphnia europaea with a tolerance toxicity domestication genotype is obtained, and the survival rate and the reduction rate of the daphnia europaea during treatment of the cylindrospora are improved; the toxicity domestication genotype daphnia blurra is used for carrying out biological algae control treatment on the cylindrotheca, and the control effect on the growth of the cylindrotheca is effectively improved.

This invention discloses a simulated ecosystem for the preservation of Daphnia macrocarpa, comprising an upward-opening main ecological tank. Within the main ecological tank, from top to bottom, are a preservation and cultivation mechanism, a food self-cultivation mechanism, and a water purification mechanism. The preservation and cultivation mechanism includes a horizontally arranged preservation and cultivation support partition fixed within the main ecological tank. Multiple preservation and cultivation tanks with vertically extending axes are arranged on the preservation and cultivation support partition. Multiple first through holes communicating with the interior of each preservation and cultivation tank are opened at the bottom. This ecosystem constructs a closed-loop preservation system simulating a natural ecosystem, achieving integrated operation of food self-sufficiency, water self-purification, and environmental self-control during the preservation of Daphnia macrocarpa. This avoids the risks of exogenous pollution and stress damage to the Daphnia macrocarpa caused by manual operation, enabling long-term stable preservation of Daphnia macrocarpa.

The application relates to the technical field of zooplankton culture, and discloses a daphnia magna propagation system and method, which comprises a pretreatment unit and a daphnia magna propagation tank. The pretreatment unit comprises a water storage bin, an overflow box and a pulverizer. A water inlet and a water outlet are arranged on the water storage bin. The overflow box is fixedly arranged in the water storage bin. The pulverizer is fixedly arranged in the overflow box. The daphnia magna propagation tank is connected with the water outlet. Water and algae for daphnia magna culture enter the water storage bin through the water inlet, are crushed by the pulverizer, and then enter the daphnia magna propagation tank through the water outlet for daphnia magna to eat. The water storage bin, the overflow box and the pulverizer can crush the algae with a particle size not meeting the requirements and add the algae into the daphnia magna propagation tank. The method can save the input cost of the algae, realize automatic addition of the algae, and improve the propagation efficiency of the algae.

The invention discloses a water toxicity early warning system and method based on biological behaviors of daphnia. The method comprises the following steps: preparing living daphnia and putting the living daphnia into a cleaned daphnia feeding observation cabin for domestication; turning on a power switch to carry out system self-inspection; the water sample pretreatment module is used for extracting and filtering a tested water body; the water bubble removing module is used for removing water bubbles through ultrasonic filtering, heating bubble removing, exhausting and cooling; a nutrient solution is regularly provided for the daphnia through the nutrient solution supply module; daphnia behaviors are observed and recorded by using a daphnia feeding observation module and a daphnia behavior video acquisition module, and a system host control module analyzes behavior data and judges whether a water sample is polluted or not; if the daphnia behavior is abnormal, an early warning signal is sent out.

The utility model provides an automatic daphnia magna cultivation and breed conservation device which comprises a daphnia magna cultivation cavity unit which comprises at least one daphnia magna cultivation cavity, a water-cooling constant-temperature cavity and a water cooling machine, the daphnia magna cultivation cavity is connected with the water-cooling constant-temperature cavity, and the water-cooling machine is connected with the water-cooling constant-temperature cavity. The water-cooling machine is communicated with the water-cooling constant-temperature cavity, the algae culture cavity unit comprises an algae culture cavity and an algae culture solution container connected with the algae culture cavity, and the algae culture cavity is communicated with the daphnia magna culture cavity; the controller is electrically connected with the algae culture cavity unit and the daphnia magna culture cavity unit. Based on the daphnia magna culture and breed conservation device, the components are uniformly regulated and controlled by the controller, the daphnia magna culture cavity unit and the algae culture cavity unit, so that the daphnia magna culture automation is realized, various indexes in the daphnia magna culture process are ensured to meet the requirements, and possible errors or interference caused by manual operation is avoided.

The invention provides a method for detecting Antp mRNA expression localization in scylla paramamosain larvae through overall fluorescence in situ hybridization and application, and belongs to the technical field of gene detection. According to the method, the scylla paramamosain larvae stored in paraformaldehyde are treated through methods of rehydration, bleaching, fixation, digestion, pre-hybridization, repeated washing after hybridization and the like, after treatment is finished, the larvae are stored with glycerol, then observation is conducted through a confocal microscope, positioning exploration of the Antp gene in the scylla paramamosain daphnia-shaped larvae is successfully achieved, the experiment operation difficulty is greatly reduced, and the experiment efficiency is improved. According to the method, the traditional fluorescence in-situ hybridization process is simplified, the detection efficiency and accuracy are remarkably improved, meanwhile, the tissue form of the larva is completely reserved, the visual positioning of Antp mRNA in each tissue organ of the scylla paramamosain larva is realized, and an efficient and economical technical scheme is provided for analyzing the development molecular mechanism of the scylla paramamosain larva.

The invention relates to the field of biotoxicity detection, in particular to a biotoxicity evaluation method and device based on heartbeat signals of daphnia organisms and a storage medium. The method comprises the following steps: acquiring heartbeat signal characteristics of daphnia organisms under the action of a to-be-detected water sample, and according to response characteristics of the heartbeat signal characteristics in the to-be-detected water sample containing different pollutants, outputting by adopting a pre-trained biotoxicity prediction model to obtain a biotoxicity evaluation result, the biotoxicity evaluation result is used for indicating the biotoxicity of the water sample to be detected. According to the embodiment of the invention, the heartbeat signal characteristics of the daphnia organisms under the action of the water sample to be detected are obtained, and the biotoxicity evaluation result is quickly output by utilizing the biotoxicity prediction model, so that the problems of low sensitivity, long detection period, complicated operation, invasive injury to the tested organisms caused by invasive detection and the like in the traditional method are solved; the rapid and high-sensitivity toxicity assessment of water pollutants is realized; and the daphnia tested organisms are noninvasive and nondestructive in the whole process.

The utility model relates to the technical field of fish culture, and discloses a composite circulating culture device which comprises a culture pond and partition plates arranged in the culture pond, and the partition plates divide the culture pond into a fish culture pond, a daphnia culture pond and an algae culture pond. The circulating pump part is used for circulating water in the fish culture pond, the daphnia culture pond and the algae culture pond, high-yield and high-efficiency culture of fishes, algae and daphnia is achieved by optimizing the culture structure and improving the resource utilization rate, and culture benefits are improved; algae and daphnia are used for purifying water, waste emission is reduced, the breeding environment is improved, and ecological balance is promoted.