263 results about "Probe target" patented technology

The present invention provides a method for detecting probe-target substrate binding. In particular, the present invention provides a method for detecting a surface bound target complex by detecting the redox reaction of a redox transition metal complex that is catalyzed by a redox-catalyst complex.

The invention relates to a method for emitting detector which can select the detected target via swing-by mechanism, belonging to the technique of deep space detecting rail transfer. It can solve the problems of approachability evaluation of detected target with long semi long axis or large eccentric rate. The inventive method comprises: calculating optimized dual impulse transfer track; selecting swing-by track type; and assembling the swing-by tracks. First calculating the optimized dual impulse transfer track from the target star to emitting star; using the emitting star as swing-by star; using the parameter of dual impulse transfer at the emitting star as the match parameter in swing-by flying; using the heliocentric large-ellipse orbit whose period is several times of the revolution period of emitting star and the deep space device at aphelion, to search for the emitting parameter of said match condition. The invention guides in the swing-by mechanism to realize the expansion of former dual impulse transfer, to improve the approachability of the target with long semi long axis or large eccentric rate.

The invention provides a ratiometric fluorescent probe targeting mitochondria for detecting sulfur dioxide/sulfite (hydrosulphite). The ratiometric fluorescent probe has a structural formula as shownin the specification. The ratiometric fluorescent probe for detecting the sulfur dioxide provided by the invention has high recognition sensitivity, eliminates the interference of background, environment and concentration at the same time, is resistant to interference of various ions, amino acids and active oxygen, and has good specificity and potential application value for detecting the sulfur dioxide in environmental and biological systems. The probe provided by the invention has the advantages of simple and practicable synthetic process, cheap and easily-available raw materials, low preparation cost and easy popularization.

The present application discloses a method for detecting a presence of target ligand in a fluid medium which includes the steps of: (i) contacting the fluid medium with a solid substrate that includes an array of dendrons on its surface, wherein each of the dendron includes a central atom, a probe that is attached to the central atom optionally through a linker, and a base portion attached to the central atom and having a plurality of termini that are attached to the surface of the solid support; and (ii) determining the presence of a probe-target ligand complex by measuring binding force between the bound ligand and detection molecule tethered to the tip of an atomic force microscope (“AFM”), which detection molecule has affinity for the ligand, wherein measurement of an increase in force between the probe-target ligand complex and the detection molecule by AFM indicates the presence of the probe-target ligand complex.

A microfluidic test module having an outer casing having an inlet for receiving a biological sample containing a target nucleic acid sequence, and, a hybridization chamber mounted in the casing, the hybridization chamber holding a volume of fluid containing probes, the probes each having a nucleic acid sequence for hybridization with the target nucleic acid sequence to form probe-target hybrids, wherein, the mass of the probes in each of the hybridization chambers is less than 270 picograms.

The invention belongs to the field of biomedicines and relates to a gene chip hybridization probe for screening disease-causing gene mutations of child glaucoma, and a design method and application thereof. The invention provides a preparation method of a gene chip and the gene chip hybrid probe related to the disease-causing gene mutations of the child glaucoma, and the gene chip hybrid probe targets genes covering 289 pathogenic genes highly associated with the child glaucoma. The design method of the gene chip hybrid probe is as follows: designing and synthesizing a child glaucoma pathogenic gene hybrid probe, and integrating the child glaucoma pathogenic gene hybrid probe into a gene chip; using the prepared gene chip to capture a genome target region and performing deep sequencing; and performing bioinformatics analysis on screening data to screen candidate pathogenic genes and mutations. A high-efficiency screening technique for screening the disease-causing gene mutations of thechild glaucoma is established, and is helpful for clinically determining the mutant genes of the child glaucoma.

A measurement targeting apparatus comprises a target body, a vessel with a channel, a fluid located within the channel in the vessel, and a pressurization system. The target body is selected from one of a photogrammetry target, a theodolite target, a construction ball, a touch probe target, a coordinating measurement machine probe target, a laser tracker target, and a laser projector target. The vessel has a centerline, has a substantially cylindrical portion capable of being received in a hole in a part, and is comprised of a material selected from steel, aluminum, and plastic. The vessel is capable of expanding when the fluid within the channel in the vessel is pressurized. The pressurization system is capable of pressurizing the fluid within the channel to cause the vessel to expand around the centerline.

Method and system to predict and optimize probe-target hybridization are provided. The method may be implemented using six interactive, interrelated, software modules. Module 1 predicts the hybridization thermodynamics of a duplex given the two strands. Module 2 finds the best primer of a given length binding to a given target. Module 3 executes a primer walk to find alternative binding sites of a given primer on a given target. Module 5 is a combination of Modules 2 and 3. Module 6 finds the alternative binding sites of a given primer on a given target (Module 3) and calculates the concentration of target with primer bound at primary and alternative sites. Module 7 is a combination of Modules 2 and 5 and also calculates the various concentrations. The six modules can be operated either through an interactive user interface or using batch file submission as provided by Module 4. The program is suited to predict DNA/DNA, RNA/RNA, and RNA/DNA systems.

The invention discloses an HER2 gene amplification detection probe, a kit and a method. The detection probe comprises a first group of probes for labeling fluorescent dye and targeted to an HER2 gene, and a second group of probes targeted to the centromere region of human chromosome 7, wherein the first group of the probes are selected from at least one of probes as shown in SEQ ID NO.41-SEQ ID NO.50; the second group of the probes are selected from at least one of probes as shown in SEQ ID NO.51-SEQ ID NO.60; and the fluorescent dyes labeled by the two groups of the probes are different in color. A FISH probe in the HER2 gene amplification detection kit provided by the invention is free from a repeated sequence, and capable of avoiding a cross reaction and undergoes hybridization recognition with an HER2 target detection fragment in the chromosome; and the probe has the advantages of high accuracy, good specificity and low false positive rate.

A microfluidic device having a probe for hybridization with a target nucleic acid sequence to form a probe-target hybrid, the probe-target hybrid having a reporting fluorophore for emitting a fluorescence signal in response to an excitation light, a detection photodiode for exposure to the excitation light and the fluorescence signal, and, a trigger photodiode for exposure to the excitation light, the trigger photodiode being configured to activate the detection photodiode after the excitation light has been extinguished.

The invention discloses a superconductive cyclotron extraction region beam measurement detection target head, which comprises a probe target head and a fixation structure. The probe target head is formed by a multi-wire probe, a ceramic block and a connecting rod. One end of each tungsten wire passes through a through hole in the ceramic block and extends into the connecting rod; each tungsten wire is fixedly arranged in the ceramic block through welding; and the ceramic block is fixedly arranged in one end of the connecting rod through welding. The fixation structure comprises an aluminum alloy connecting piece and a drive rod. The connecting rod and the drive rod are connected through the aluminum alloy connecting piece. The detection target head can carry out measurement based on intensity of electrical signals on different wires and through a point motion mode of the drive rod to obtain axial and radial position information of the beam, so that beam debugging can be carried out, and stable and effective operation of an accelerator is realized; and the detection target head has the advantages of high resolution, high precision, simple measurement and capable of replacing the probe without opening the cavity.

The invention discloses a device specially used for sonar-detecting whether large yellow croakers escape from a deepwater net-cage, comprising a computer, a probe which is arranged closely to the net-cage, and a digital to analog adaptive device which is connected with the computer; wherein, the probe is arranged at the external side of the net-cage; the probe comprises a sonar transducer and a flowmeter probe; the digital to analog adaptive device is connected with the probe and can convert the analog signal of the probe into the digital signal and send the signal to the computer; the computer receives the digital signal of the digital to analog adaptive device, processes the digital signal and judges whether the fish escapes or not according to the coupling result of the echo signal scanned by the flowing speed and sonar. The invention also discloses a sonar detection method which utilizes the device to detect whether the fish escapes from the deepwater net-cage simultaneously. The device and the method has the advantages that the device is not affected by water quality, the detection distance is far, the result is exact, the target objects can be intuitively and effectively monitored and detected, and the like.

A probe-target reaction is made more recognizable by the provision of a mass-enhancing and/or evanescent-field-perturbing amplifier element which reacts uniquely with and binds to the probe-target pair to provide increased mass. Where the probe-target pair is hybridized dsDNA, a suitable mass-enhancing amplifier is anti-double stranded DNA mouse IgM. In examples with sufficient sequence pairs in the probe-target combination, a sequence-specific minor-groove-binding polyamide can be used that carries biotin which can be amplified by streptavidin in a suitable carrier. In a preferred embodiment, a plurality of probes are immobilized at the sites of a microarray, each probe being specific to a different target. Optics utilizing total internal reflection are described for observing perturbation of the evanescent field.

A microfluidic device for simultaneous detection of multiple conditions in a patient, the microfluidic device having an inlet for receiving a sample of biological material drawn from the patient, a microsystems technologies (MST) layer with a detection section having an array of probes for reaction with target molecules in the sample to form probe-target complexes, the target molecules being indicative of medical conditions in the patient, and, a photosensor for detecting the probe-target complexes, wherein, the array of probes has more than 1000 probes.

Background luminescence is reduced from a solution containing unbound luminescent probes, each having a first molecule that attaches to a target molecule and having an attached luminescent moiety, and luminescent probe/target adducts. Quenching capture reagent molecules are formed that are capable of forming an adduct with the unbound luminescent probes and having an attached quencher material effective to quench luminescence of the luminescent moiety. The quencher material of the capture reagent molecules is added to a solution of the luminescent probe/target adducts and binds in a proximity to the luminescent moiety of the unbound luminescent probes to quench luminescence from the luminescent moiety when the luminescent moiety is exposed to exciting illumination. The quencher capture reagent does not bind to probe molecules that are bound to target molecules and the probe/target adduct emission is not quenched.

The invention provides an application of core-shell nanoparticles for preparing biological imaging agents, molecular biological probes and targeted drug-carrying carriers. The core-shell nanoparticle is that the inner core material is gold nanorod; the middle layer is silicon dioxide and surface acceptor; the outer shell is a coupling body of water-based CdTe quantum dots and biomolecules. The core-shell nanoparticle has low toxicity and low cost, solves the problem of water solubility of the nanoparticle, and improves the biocompatibility of the composite nanoparticle. The core-shell nanoparticles have strong fluorescence characteristics, and the surface receptors have spectral signals, and the fluorescence signals are observed through the biological imaging system to obtain the information of the lesion. Utilizing the strong fluorescent properties of the core-shell nanoparticles, they can be combined with cells in living organisms as biological probes for early diagnosis of diseases. The complex of drug and core-shell nanoparticles will successfully reside on the surface of cancer cells, and the drug will reach the cancer cells to achieve therapeutic effect.

The present disclosure provides a kit and method for detecting DNA methylation by a triplex qPCR assay. The kit includes a combination of three sets of primers and probes, wherein two sets of primersand probes are combined to target two regions selected from the sequence of the human SEPT9 gene. Another set of primers and probes targets an internal reference. The kit described in the present disclosure is capable of more sensitive and accurate determination of the DNA methylation level or status in a particular region.

A process is provided for identifying a complementary target nucleic acid. The process includes the hybridization of a nucleic acid probe to a carrier to form a nucleic acid probe-carrier complex. The complex is placed in a compartment bounded by a media permeable to the nucleic acid probe and exclusive of both the carrier and the complex. The complex is then denatured, with the nucleic acid probe transported through the media and into contact with the target nucleic acid. The nucleic acid probe hybridizes to the complementary target nucleic acid to yield a probe-target double stranded complex. A non-complementary nucleic acid probe, independent probe-target complex is returned to the compartment and given an opportunity to rehybridize to the carrier. A determination as to whether at least one of the complementary target nucleic acid or the carrier is present as a complex provides information as to probe sequences complementary to the target nucleic acid.

The invention discloses a TOP2A gene abnormality detection probe, a kit and a method. The detection probe comprises a first group of probes for labeling fluorescent dye and targeted to a TOP2A gene, and a second group of probes targeted to the centromere region of human chromosome 7, wherein the first group of the probes are selected from at least one of probes as shown in SEQ ID NO.41-SEQ ID NO.50; the second group of the probes are selected from at least one of probes as shown in SEQ ID NO.51-SEQ ID NO.60; and the fluorescent dyes labeled by the two groups of the probes are different in color. A FISH probe in the TOP2A gene abnormality detection kit provided by the invention is free from a repeated sequence, and capable of avoiding a cross reaction and undergoes hybridization recognition with a TOP2A target detection fragment in the chromosome; and the probe has the advantages of high accuracy, good specificity and low false positive rate.

The invention discloses a method for generating broadband dechirp echoes based on sweep frequency data, and belongs to the technical field of radar signal processing. The method comprises the steps: enabling a pulse radar to send a linear frequency modulation pulse signal to a detection target comprising Q scattering centers; enabling each scattering center in the detection target to feed back signals to the radar to obtain broadband echo signals of Q scattering centers; setting a reference signal, and performing dechirping processing on the broadband echo signal of each scattering center to obtain a frequency response containing a target RCS characteristic; carrying out fast Fourier inverse transformation on the frequency response containing the RCS characteristics of the target, carryingout inverse transformation on each scattering center to obtain a complex amplitude, and forming a one-dimensional range profile of the detection target by Q complex amplitudes; multiplying the one-dimensional range profile of the detection target by the dechirping echo signal of each scattering center, and performing accumulating and summing to finally obtain the broadband dechirping echo of thewhole detection target. According to the method, the operand is greatly reduced, and meanwhile, the precision and the accuracy which are the same as those of convolution operation are ensured.