45results about How to "High coincidence rate" patented technology

The invention discloses a single-nucleotide polymorphism (SNP) detection specific primer of the CYP2C19 gene and a liquid phase chip. The liquid phase chip contains an ASPE primer composed of a tag sequence on the 5'-end and specific primers on the 3'-end which aims at the target genetic mutation, microspheres covered by the anti-tag sequence and an amplification primer, wherein the specific primers contain SEQ ID NO.25 and SEQ ID NO.26, SEQ ID NO.27 and SEQ ID NO.28 aiming at the C194T SNP site, SEQ ID NO.29 and SEQ ID NO.30, SEQ ID NO.31 and SEQ ID NO.32, SEQ ID NO.33 and SEQ ID NO.34, SEQID NO.35 and SEQ ID NO.36, SEQ ID NO.37 and SEQ ID NO.38, SEQ ID NO.39 and SEQ ID NO.40, SEQ ID NO.41 and SEQ ID NO.42, SEQ ID NO.43 and SEQ IDNO.44, SEQ ID NO.45 and SEQ ID NO.46, and / or SEQ ID NO.47 and SEQ ID NO.48. The coincidence rate of the detection result of the SNP detection liquid phase chip of the CYP2C 19 gene and the detection result adopting a sequencing method is up to 100%.

The invention provides a CYP2D6 gene mutation detection liquid-phase chip which comprises ASPE (Allele Specific Primer Extension) primers aiming at CYP2D6 C2850T and CYP2D6Deletion mutational sites, three microballoons respectively enveloped with a specific anti-tag sequence and amplification primers aiming at the CYP2D6C2850T and the CYP2D6 Deletion mutational sites. The CYP2D6 gene mutation detection liquid-phase chip can simultaneously detect aiming at the CYP2D6 C2850T and the CYP2D Deletion mutational sites and has excellent signal to noise ratio. The coincidence ratio with a sequencing method of the CYP2D6 gene mutation detection liquid-phase chip reaches up to 100 percent, and the CYP2D6 gene mutation detection liquid-phase chip has higher specificity and precision compared with intra-class correlation products.

The invention discloses papillomavirus detection and a typing liquid chip, and a process which is used to the detection and typing papillomavirus. The liquid chip mainly comprises microsphere which is respectively enveloped with specific anti-tag label sequence and 7-10 T spacer arm sequence which is arranged between anti-tag label sequence and microsphere, wherein anti-tag label sequence is chosen from two or more than two sequences in SEQ ID NO. 25- SEQ ID NO.47, specific ASPE primer is respectively designed aiming at each typing HPV, ASPE primer is chosen from two or more than two sequencefrom SEQ ID NO.1-SEQ ID NO.23, and primer of target sequence which has mutant sites is amplified. The liquid chip improves the current liquid chip technology, which makes ribonucleotide probe microspheres which is prepared be suitable to different detection projects, largely increases fluorescent signal value which is detected, thereby further increasing sensitivity of the detection, strengthening signal-to-noise ratio, and making detection results more accurate and reliable.

The invention discloses a C6ORF97 gene mutation detection liquid chip, and specific primers. The C6ORF97 gene mutation detection liquid chip mainly comprises: ASPE primers, wherein each ASPE primer is composed of 5'-terminal tag sequence, and 3'-terminal specific primer sequence targeting target gene mutation sites, and the specific primer sequence comprises SEQ ID No.9 and SEQ ID No.10 targeting C1810T site, SEQ ID No.11 and SEQ ID No.12 targeting T91235C site, SEQ ID No.13 and SEQ ID No.14 targeting A68C site, and / or SEQ ID No.15 and SEQ ID No.16 targeting A123001G site; microballoons coated with anti-tag sequence; and amplification primers. Self-agreement ratio of detection results of the liquid chip with detection results of sequencing is as high as 100%; and parallel detection of wild types and mutant types of a plurality of mutation sites is realized.

The invention discloses specific primes and a liquid phase chip for AGT (angiotensinogen) and ACE (angiotensin-converting enzyme) gene polymorphism detection. The liquid phase chip mainly contains ASPE (allele specific primer extension) which consists of tag sequences at 5' end and specific primers at 3'-end relative to a target gene mutation, wherein the specific primers respectively are SEQ ID NO. 9 and SEQ ID NO. 10, SEQ ID NO. 11 and SEQ ID NO. 12, SEQ ID NO. 13 and SEQ ID NO. 14 regarding to relative to the AGT gene, and / or SEQ ID NO. 15 and SEQ ID NO. 16 relative to the ACE gene; microspheres coated with anti-tag sequences; and amplification primers. The conformation between the detection result of the AGT and ACE gene polymorphism detection liquid phase chip and the result of a sequencing method is up to 100%. The prepared liquid phrase has excellent signal-noise ratio and can avoid cross reaction and realize the parallel detection of multiple polymorphic sites.

The invention discloses ABCB2 gene detection specific primers and a liquid chip. The liquid chip mainly comprises: various ASPE primers composed of tag sequence and specific primer sequences aiming at mutation site, microspheres coated with anti-tag sequence, and amplification primers. The specific primer sequences comprise: SEQ ID NO.13 and SEQ ID NO.14 aiming at G142A site; SEQ ID NO.15 and SEQ ID NO.16 aiming at G88A site; SEQ ID NO.17 and SEQ ID NO.18 aiming at G154A site; SEQ ID NO.19 and SEQ ID NO.20 aiming at C86T site; SEQ ID NO.21 and SEQ ID NO.22 aiming at C150T site; and / or SEQ ID NO.23 and SEQ ID NO.24 aiming at A92T site. The matching rate of the detection result obtained by using the liquid chip provided by the invention and a result obtained by using a sequencing method is 100%. Therefore, wild-type and mutant-type parallel detections of a plurality of mutation sites are realized.

The invention discloses BRAP and PSMA6 gene SNP detection specific primers and a liquid phase chip. The liquid phase chip comprises an ASPE primer which is composed of a tag sequence at a 5' terminal and specific primers mutated for a target gene at a 3' terminal, wherein the specific primers respectively comprise a SEQ ID NO.7 and a SEQ ID NO.8 for a BRAP gene A96G SNP locus, a SEQ ID NO.9 and a SEQ ID NO.10 for a BRAP gene A80G SNP locus, and / or a SEQ ID NO.11 and a SEQ ID NO.12 for a PSMA6 gene C157G SNP locus; microspheres coated by an anti-tag sequence; and amplification primers. The coincidence rate between detection results of the BRAP and PSMA6 gene SNP detection liquid phase chip provided by the invention and results of a sequencing method is up to 100%.

The invention discloses a carbonate rock surface fracture reservoir body characterization method. The method comprises the steps: calculating a root mean square amplitude property in a first depth range of the carbonate rock surface and calculating a relative wave impedance property in a second depth range; establishing a regression function according to the calculated two properties, converting one attribute into the other attribute according to the regression function, depicting a fracture reservoir body according to the values of the converted and unconverted attributes to obtain two piecesof fracture reservoir body data, and performing the coupling of the fracture reservoir body data to obtain a coupled data body of the carbonate rock surface fracture reservoir body. The method solvesthe problem of characterization loss caused by using the root mean square amplitude attribute to characterize the surface layer of a carbonate rock, improves the coincidence rate between a characterization result and actual drilling, and provides reliable basis for the potential tapping of the residual oil and improving the recovery of the fracture-cavity type reservoir.

The invention provides a method and a chip for detecting multidrug-resistant mycobacterium tuberculosis. The method and chip of the present invention relate to the detection of katG315 mutant type I and / or II, rpoB526 mutant type I and / or II, rpoB531 mutant type I and / or II, and inhA-15 mutant type. The present invention also provides primers for detecting the above-mentioned mutants.

The invention discloses specific primers and a liquid-phase chip for SNP (Single Nucleotide Polymorphism) detection of MTHFR and FGF5 genes. The liquid-phase chip comprises an ASPE primer, anti-tag sequence coated microspheres, and an amplification primer, wherein the ASPF primer is composed of a 5'-terminal tag sequence and 3'-terminal specific primers for target gene mutation, and the specific primers are respectively SEQ ID NO. 9 and SEQ ID NO. 10 specific for a G121A SNP site, SEQ ID NO. 11 and SEQ ID NO. 12 specific for an A105G SNP site, SEQ ID NO. 13 and SEQ ID NO. 14 specific for a G111A SNP site, and / or SEQ ID NO. 15 and SEQ ID NO. 16 specific for a T196A SNP site of the FGF5 gene. According to the invention, the coincidence rate between the detection result of the liquid-phase chip for SNP detection of the MTHFR and FGF5 gene and the detection result of a sequencing method is up to 100%.

The invention relates to a DNA tag and a pair of PCR primers connected to the DNA tag at the 5' end. The invention also provides a method and a kit for constructing a sequencing library consisting of the DNA tag and the pair of PCR primers. The DNA tag provided by the present invention and the amplification primer form a detection product that achieves optimization and balance between specificity, sensitivity and repeatability, and can detect 1-20 samples from different sources at a time, and can accurately distinguish samples from various sources. For the base sequence, the coincidence rate between the high-throughput sequencing described in the present invention and the sequencing method is as high as 100%.

The invention discloses a liquid phase chip and a specific primer for detecting RAD51L1 gene mutation. The liquid phase chip mainly comprises: ASPE primers, microspheres coated by different anti-tag sequences, and an amplification primer, wherein each ASPE primer is composed of a tag sequence at a 5' end and specific primer sequences at a 3' end and aiming at target gene mutation sites, and the specific primer sequences are as follows: SEQ ID NO.9 and SEQ ID NO.10 aiming at a C167T site, SEQ ID NO.11 and SEQ ID NO.12 aiming at an A76T site, SEQ ID NO.13 and SEQ ID NO.14 aiming at a T187G site, and / or SEQ ID NO.15 and SEQ ID NO.16 aiming at a T99G site. The coincidence rate of the detection result of the detection liquid phase chip disclosed by the invention with that of a sequencing method reaches as high as 100%, and parallel detection of a wild type and a mutant of a plurality of mutation sites is achieved.

The invention discloses specific primers and a liquid-phase chip for SNP (Single Nucleotide Polymorphism) detection of a chromosome 4q25 section. The liquid-phase chip comprises an ASPE primer, anti-tag sequence coated microspheres, and an amplification primer, wherein the ASPF primer is composed of a 5'-terminal tag sequence and 3'-terminal specific primers for target gene mutation, and the specific primers are respectively SEQ ID NO. 11 and SEQ ID NO. 12 specific for a C172T SNP site, SEQ ID NO. 13 and SEQ ID NO. 14 specific for a C133A SNP site, SEQ ID NO. 15 and SEQ ID NO. 16 specific for a T139G SNP site, SEQ ID NO. 17 and SEQ ID NO. 18 specific for a T80A SNP site, and / or SEQ ID NO. 19 and SEQ ID NO. 20 specific for a T105C SNP site. According to the invention, the coincidence rate between the detection result of the liquid-phase chip for SNP detection of the chromosome 4q25 section and the detection result of a sequencing method is up to 100%, not only is the condition of single site mutation detected, but also the polymorphism conditions of multiple mutant sites can be simultaneously detected in parallel, and the detection effects are consistent.

The invention provides a liquid-phase chip for chromosome 13q22 region SNP detection. The liquid-phase chip mainly comprises: ASPE primers composed of tag sequences of 5' end and sequences of specific primers for SNP sites of target genes of 3' end, wherein the sequences of the specific primers are SEQ ID No.1 and SEQ ID No.2 for A94G site, and / or SEQ ID No.3 and SEQ ID No.4 for G288A site; microballoons coated by anti-tag sequences; and amplimers. The liquid-phase chip for chromosome 13q22 region SNP detection and a sequencing method reach 100% agreement in detection results, and parallel detection of wide type and SNP type of a plurality of SNP sites can be realized.