45results about How to "High coincidence rate" patented technology

The invention discloses an area-of-interest detection method based on Kinect. The area-of-interest detection method is used for solving the technical problem that an existing area-of-interest detection method based on an improved saliency map model is low in accuracy. According to the technical scheme, a Kinect3D camera lens is used for obtaining a two-dimensional RGB image and depth information; on the basis, the RGB image is used for extracting various visual features and establishing a multi-scale visual feature map; then the feature map and a depth map are fused to generate the saliency map, and a winner-take-all strategy is used for generating a two-value saliency map; finally, dilation is performed on the two-value saliency map to detect a final area of interest. The 3D image, generated by the Kinect camera lens, in a RGB-D format can be used for detecting the area of interest, wherein the area of interest is consistent with the result of perception through the human eyes. Under the same condition, the matching rate of the area of interest is increased to 91.2% from 82.5% in the background technology, which means the matching rate is increased by 8.7%, and the area of interest is automatically detected by the utilization of the method.

The invention discloses papillomavirus detection and a typing liquid chip, and a process which is used to the detection and typing papillomavirus. The liquid chip mainly comprises microsphere which is respectively enveloped with specific anti-tag label sequence and 7-10 T spacer arm sequence which is arranged between anti-tag label sequence and microsphere, wherein anti-tag label sequence is chosen from two or more than two sequences in SEQ ID NO. 25- SEQ ID NO.47, specific ASPE primer is respectively designed aiming at each typing HPV, ASPE primer is chosen from two or more than two sequence from SEQ ID NO.1-SEQ ID NO.23, and primer of target sequence which has mutant sites is amplified. The liquid chip improves the current liquid chip technology, which makes ribonucleotide probe microspheres which is prepared be suitable to different detection projects, largely increases fluorescent signal value which is detected, thereby further increasing sensitivity of the detection, strengthening signal-to-noise ratio, and making detection results more accurate and reliable.

The invention provides a CYP2D6 gene mutation detection liquid-phase chip which comprises ASPE (Allele Specific Primer Extension) primers aiming at CYP2D6 C2850T and CYP2D6Deletion mutational sites, three microballoons respectively enveloped with a specific anti-tag sequence and amplification primers aiming at the CYP2D6C2850T and the CYP2D6 Deletion mutational sites. The CYP2D6 gene mutation detection liquid-phase chip can simultaneously detect aiming at the CYP2D6 C2850T and the CYP2D Deletion mutational sites and has excellent signal to noise ratio. The coincidence ratio with a sequencing method of the CYP2D6 gene mutation detection liquid-phase chip reaches up to 100 percent, and the CYP2D6 gene mutation detection liquid-phase chip has higher specificity and precision compared with intra-class correlation products.

The invention discloses a PCR (Polymerase Chain Reaction) primer, a kit and a liquid phase chip for detecting an ALK (Anaplastic Lymphoma Kinase) fusion gene. The liquid phase chip comprises a PCR amplification primer, ASPE (Allele Specific Primer Extension) primers and microspheres, wherein the ASPE primers consist of tag sequences and specific primers, wherein the sequences of the specific primers are SEQ ID NO.12 for K15; DEL15A20, SEQ ID NO.13 for K17; A20, SEQ IDNO.14 for K9; A20, SEQ ID NO.15 for T6; A20 and/or SEQ ID NO.16 for T3; A20. The liquid phase chip disclosed by the invention has a very good signal noise ratio and can amplify five fusion subtypes by a single step, and the specific primers have very good specificity.

The invention discloses an ALK gene rearrangement detection probe, a kit and a method. The detection probe comprises a first group of probes for labeling fluorescent dye and targeted to the upstream side of a breaking point, and a second group of probes targeted to the downstream side of the breaking point, wherein the first group of the probes are selected from at least one of probes as shown in SEQ ID NO.41-SEQ ID NO.50; the second group of the probes are selected from at least one of probes as shown in SEQ ID NO.51-SEQ ID NO.60; and the fluorescent dyes labeled by the two groups of the probes are different in color. A FISH probe in the ALK gene rearrangement detection kit provided by the invention is free from a repeated sequence, and capable of avoiding a cross reaction and undergoes hybridization recognition with an ALK target detection fragment in a chromosome; and the probe has the advantages of high accuracy, good specificity and low false positive rate.

The present invention discloses an oil-gas microorganism gene exploration method. The method comprises the following steps: respectively collecting samples of surface shallow layers above a known oilwell, a gas well and a dry well in an exploration area, performing high-throughput sequencing after extracting DNA, establishing a microbial community composition pattern diagram in the exploration area according to a sequencing result, respectively screening out characteristic microorganisms in surface soil above the oil/gas well in the exploration area according to the pattern diagram, and designing primers according to attribute characteristics and carrying out a fluorescent quantitative PCR detection on the samples in the whole exploration area to detect the number of the characteristic microorganisms. A contour line of the characteristic microorganisms obtained by the oil-gas microorganism gene exploration method has a high coincidence rate with the known well, has a good coincidencerate with a trap line, and can carefully describe an oil area.

The invention discloses a carbonate rock surface fracture reservoir body characterization method. The method comprises the steps: calculating a root mean square amplitude property in a first depth range of the carbonate rock surface and calculating a relative wave impedance property in a second depth range; establishing a regression function according to the calculated two properties, converting one attribute into the other attribute according to the regression function, depicting a fracture reservoir body according to the values of the converted and unconverted attributes to obtain two piecesof fracture reservoir body data, and performing the coupling of the fracture reservoir body data to obtain a coupled data body of the carbonate rock surface fracture reservoir body. The method solvesthe problem of characterization loss caused by using the root mean square amplitude attribute to characterize the surface layer of a carbonate rock, improves the coincidence rate between a characterization result and actual drilling, and provides reliable basis for the potential tapping of the residual oil and improving the recovery of the fracture-cavity type reservoir.

The invention discloses BRAP and PSMA6 gene SNP detection specific primers and a liquid phase chip. The liquid phase chip comprises an ASPE primer which is composed of a tag sequence at a 5' terminal and specific primers mutated for a target gene at a 3' terminal, wherein the specific primers respectively comprise a SEQ ID NO.7 and a SEQ ID NO.8 for a BRAP gene A96G SNP locus, a SEQ ID NO.9 and a SEQ ID NO.10 for a BRAP gene A80G SNP locus, and/or a SEQ ID NO.11 and a SEQ ID NO.12 for a PSMA6 gene C157G SNP locus; microspheres coated by an anti-tag sequence; and amplification primers. The coincidence rate between detection results of the BRAP and PSMA6 gene SNP detection liquid phase chip provided by the invention and results of a sequencing method is up to 100%.

The invention provides a method and a chip for detecting multidrug-resistant mycobacterium tuberculosis. The method and the chip disclosed by the invention relate to detection of katG315 mutant I and/or II or rpoB526 mutant I and/or II, rpoB531 mutant I and/or II, and inhA-15 mutant. The invention also provides a primer for detecting the mutants.

The invention discloses specific primers and a liquid-phase chip for SNP (Single Nucleotide Polymorphism) detection of MTHFR and FGF5 genes. The liquid-phase chip comprises an ASPE primer, anti-tag sequence coated microspheres, and an amplification primer, wherein the ASPF primer is composed of a 5'-terminal tag sequence and 3'-terminal specific primers for target gene mutation, and the specific primers are respectively SEQ ID NO. 9 and SEQ ID NO. 10 specific for a G121A SNP site, SEQ ID NO. 11 and SEQ ID NO. 12 specific for an A105G SNP site, SEQ ID NO. 13 and SEQ ID NO. 14 specific for a G111A SNP site, and/or SEQ ID NO. 15 and SEQ ID NO. 16 specific for a T196A SNP site of the FGF5 gene. According to the invention, the coincidence rate between the detection result of the liquid-phase chip for SNP detection of the MTHFR and FGF5 gene and the detection result of a sequencing method is up to 100%.