The invention discloses a
gene methylation detection method, a detection kit and an application thereof.
Gene methylation detection sensitivity is improved by two strands of the same
gene for the first time, namely, a positive-
sense strand and antisense strand
methylation fluorescent accumulating method. Compared with an existing methylation detection technology, the method for detecting
gene methylation degrees by the two strands has the
advantage of higher gene methylation detection sensitivity. The two strands of
DNA (deoxyribonucleic acid) are sufficiently used, fluorescent
signal values are increased, methylation
fluorescence is double accumulated, the gene methylation detection sensitivity is improved, and the methylation degrees can be distinguished by a qualitative method. Besides, the detection process is reaction in closed pipes with the same hole, namely, methylation of the two strands can be simultaneously detected in one hole, operation is simple and rapid, and the possibility of
pollution is decreased. Safety is achieved, the whole
system is free from toxic and harmful substances, open pipes of PCR (
polymerase chain reaction) products are omitted, and testers and environments are not harmed.