BRAP and PSMA6 gene SNP detection specific primer and liquid phase chip

Abstract

The invention discloses BRAP and PSMA6 gene SNP detection specific primers and a liquid phase chip. The liquid phase chip comprises an ASPE primer which is composed of a tag sequence at a 5' terminal and specific primers mutated for a target gene at a 3' terminal, wherein the specific primers respectively comprise a SEQ ID NO.7 and a SEQ ID NO.8 for a BRAP gene A96G SNP locus, a SEQ ID NO.9 and a SEQ ID NO.10 for a BRAP gene A80G SNP locus, and / or a SEQ ID NO.11 and a SEQ ID NO.12 for a PSMA6 gene C157G SNP locus; microspheres coated by an anti-tag sequence; and amplification primers. The coincidence rate between detection results of the BRAP and PSMA6 gene SNP detection liquid phase chip provided by the invention and results of a sequencing method is up to 100%.

Description

technical field [0001] The invention belongs to the field of molecular biology, relates to medicine and biotechnology, in particular to a specific primer for detecting BRAP and PSMA6 gene SNP and a liquid phase chip. Background technique [0002] Breast cancer susceptibility gene 1 associated protein gene (breast cancer susceptibility gene 1 associated protein, BRAP), located at 12q24 of the human genome, is named because its encoded protein can bind to the nuclear localization signal region of BRCA1. BRAP is a cytoplasmic protein whose main physiological function is to regulate the nuclear targeting of other proteins; in addition, BRAP is also associated with inflammation-related molecules lymphotoxin-α and galectin-2. [0003] Studies have shown that lymphotoxin-α is an important pro-inflammatory factor, involved in the release of chemokines, adhesion and aggregation between cells, etc., and plays an important role in the occurrence and development of cardiovascular diseas...

AI Technical Summary

Problems solved by technology

The PCR-RFLP method is based on the change of the restriction endonuclease recognition site caused by gene mutation, such as the loss of the site or the generation of a new site, a specific fragment is amplified by PCR, and then cut and amplified with a restriction endonuclease. Amplify the product and observe the size of the fragment by electrophoresis. This method is used to detect gene mutations with altered restriction sites, and can directly determine the genotype. However, this method cannot be used for the detection of gene mutations without new restriction sites.

Thirdly, both PCR-based detection methods (PCR-RFLP) and direct sequencing methods have limitations in detection throughput, and only one mutation can be detected at a time, which cannot meet the needs of practical applications.

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