1 results about "Cytoplasmic protein" patented technology

The invention discloses a low-abundance cell nucleus enrichment method which comprises the following steps: (1) splitting a tissue sample to obtain a cell nucleus with extracellular cytoplasm retained; and (2) marking and sorting the cell nucleuses obtained in the step (1) to obtain enriched low-abundance cell nucleuses. According to the method, a mild dissociation technology is used, so that more cytoplasm components can be reserved in cell nucleuses, and then the cell nucleuses are used for marking cytoplasm proteins and are combined with flow sorting, so that directional enrichment of the cell nucleuses with low abundance and specific functional states can be effectively realized. Through the whole process optimization, the method can ensure the completeness of the cell nucleus to the maximum extent, takes downstream sequencing application into consideration, and can obtain high-quality mononuclear transcriptome sequencing data by combining with the screening of cytoplasm sequencing data. Moreover, according to the method, directional enrichment is realized through the cytoplasmic protein, the use cost can be effectively reduced, and the method is suitable for large-scale clinical sample data acquisition and has higher flux compared with a single space transcriptome slice.