45 results about "Protein dna" patented technology

The present invention discloses a fusional protein of a serum albumin and an interferon, the coding of the fusional protein DNA sequence, as well as bacteria, barm, animal cell and plant cell of the DNA sequence. The fusional protein provided by the present invention includes a first district with at least 85 percents congeneric sequence of the artificial serum albumin and a second district with at least 85 percents congeneric sequence of the artificial interferon. Without changing the property of the fusional protein, the fusional protein can implement the replacement, deficit or addition ofexceptional amide acid residue. A connecting peptide is arranged between the first district and the second district of the fusional protein provided by the present invention; the general formula of the connecting peptide is [GlyGly GlyGlySer]n and n is an integer from 1 to 10. The fusional protein provided by the present invention with the property of artificial interferon and prolonged half-value period will be widely applied in the medicine field.

The invention relates to a method for determining a transcriptional control complex, and belongs to the field of molecular biology. The method for determining the transcriptional control complex includes the following steps: preparation of a bait DNA sequence, preparation of a DNA sequence for encoding a bar code protein, construction of a bar code protein library, screening of the transcriptional control complex, and in situ adjacent connection capture of the transcriptional control complex, and the identification of the transcriptional control complex. The invention combines cDNA display technology and the adjacent connection technology to establish a novel method for determining the transcriptional control complex; the determination of transcriptional control complex involves simultaneous determination of a variety of interaction effects of protein-protein and protein-DNA; and the method can realize simultaneous determination of a variety of interaction effects of protein-protein and protein-DNA in a same experimental system, and effectively solve the incompatibility of the determination methods for the protein-protein interaction and protein-DNA interaction in the same experimental system in the prior art.

The invention provides a recombinant sheep prion protein oPrP and a preparation method and application thereof. Amino acid sequences of the protein oPrP are shown in SEQ ID No.2. The preparation method includes: using a whole-blood sheep gene as a template, obtaining a sheep prion protein DNA (deoxyribonucleic acid) sequence with enzyme cutting sites by using nucleotide sequences shown in SEQ ID No 3 and 4 as primers and by a PCR (polymerase chain reaction) method, connecting the DNA sequence onto pPROEX-htb plasmids, and removing redundant enzyme cutting sites before a target gene in an expression vector by site-directed mutagenesis, so that an oPrP entire expression vector is obtained; and expressing the vector in escherichia coli, and purifying and renaturing by an overhigh affinity chromatographic column so that the recombinant sheep prion protein is obtained. The amino acid sequences of the obtained recombinant sheep prion protein are highly similar to those of a natural sheep prion protein (only an N terminal comprises one more glycine) and can be used for preparing sheep prion protein monoclonal antibodies and research and development of scrapie diagnostic reagent kits.

Provided are an antiacid nano particle oral deoxyribonucleic acid (DNA) anti-tumor vaccine with the potential of hydrogen (pH) sensitive characteristic and a preparation method. The DNA oral vaccine is nano particles formed by combining chitosan with alginic acid for surface finish and encoded tumor specific antigen Legumain protein DNA plasmids. The DNA oral vaccine is capable of being efficiently phagocytized by dendritic cells and macrophage in peyer's patches and expressing the encoded tumor antigen to activate immune damage of a host for tumor cells. The nano particles have small toxic and side effects and a strong antigen presentation role. Specifically, the DNA plasmids and chitosan are led to be in static negative crosslinking, and then, alginic acid is used for surface finish of chitosan nano particles. By means of immunofluorescent staining and flow cytometry, growth and transfer conditions of breast cancer of a mouse are estimated, immune response of the mouse is analyzed, and antigen presentation capability and anti-tumor effect of the antiacid nano particle oral DNA anti-tumor vaccine with the potential of pH sensitive characteristic are determined.

The invention discloses a preparation method of human galectin-9 deletant for enhancement of immune response, which adopts an expression vector with a protein DNA sequence and a host cell transformed with the expression vector. The invention also provides application of the human galectin-9 deletant in improvement of cellular immunity. The protein has the function of promoting the activation of DC cells and macrophages, the demonstration of the function of the protein is the enhancement of the cellular immune response, and the immunity is suppressed in case of lack of the human galectin-9 holoprotein.

The invention discloses a side unit used for building TALE (transcription activator-like effector) repeated sequences. The side unit is a repeated unit DNA (deoxyribonucleic acid) segment containing isocaudarner or different flat terminase identification sites at two ends, and the preated unit DNA segment codes repeated units of RVD (repeated variable di-residue) containing NI, NG, HD, NK or NN or variants of the repeated units, wherein in the 5'end isocaudarner or flat terminase identification sites, the 3' end of the identification sites at least has one nucleotide participating in the amino acid coding the N end of the side unit; and in the 3' end isocaudarner or flat terminase identification sites, the 5' end of the identification sites at least has one nucleotide participating in the amino acid coding the C end of the side unit. The side unit has the advantages that the TALE repeated sequences containing any repeated unit number and any ranging sequences, plasmid vectors containing TALE repeated sequences, coding TALE protein DNA combined structural domains and various derived fusion protein plasmid vectors can be conveniently built.

The invention relates to a mutant-type Sus scrofa swine trypsin and an encoding gene thereof as well as an acquisition method and an application. According to the invention, the original wild type Sus scrofa swine trypsin is subjected to mutation to obtain the mutant enzyme with heat stability obviously improved; the invention further provides the encoding gene for encoding the trypsin mutant, the amino acid sequence and a construction method of a trypsin yeast engineering bacteria, wherein the amino acid sequence is SEQ ID NO.1, and the protein DNA sequence in encoding is SEQ ID NO.2. The invention lays a foundation for the research, development and production of the protease through a bioanalysis method, and is beneficial for the early realization of the clean and low-energy biological tanning, and the bio-safe production of leather. With the strain for producing swine trypsin, the yield is relatively high, the process is relatively simple, and the industrial application is facilitated.