54results about How to "Increase library capacity" patented technology

The invention discloses a shuttle shelf type flue-cured tobacco lamina formula warehouse logistics automation system. A warehousing stacker roadway for running of a warehousing stacker is arranged between a warehousing system and a shuttle shelf, an ex-warehouse stacker roadway is for running of an ex-warehouse stacker is arranged between an ex-warehouse feeding system and the shuttle shelf, the warehousing system comprises a warehousing conveying line in butt joint with the warehousing stacker, the ex-warehouse feeding system comprises a flue-cured tobacco lamina packet feeding sequence arrangement buffer in butt joint with the ex-warehouse stacker, the flue-cured tobacco lamina packet feeding sequence arrangement buffer is connected with an unpacking machine through a feeding conveying line, a flue-cured tobacco lamina packet clamping and holding machine is arranged at the position, between the flue-cured tobacco lamina packet feeding sequence arrangement buffer and the unpacking machine, on the feeding conveying line, the shuttle shelf is provided with a plurality of passages with multiple goods allocations, and the warehousing stacker and the ex-warehouse stacker are in butt joint with a goods inlet end and a goods outlet end of each passage respectively. The system achieves dense storage and automatic warehousing and ex-warehouse of flue-cured tobacco lamina packets, and is less in investment and capable of flexibly and conveniently improving warehousing and ex-warehouse capacity as required.

The invention relates to a single-chain antibody against fenitrothion and a preparation method thereof. A ribosome display library of a whole set of single-chain antibody genes against the fenitrothion is constructed by using a ribosome display technology and rounding a hybrid tumor technology, and three single-chain antibody genes with high affinity and high specificity for the fenitrothion are screened from the library. After the single-chain antibody genes are connected with vectors PPOW3.0 and then subjected to soluble expression in escherichia coli, three soluble proteins with molecular weights of about 30KDa are obtained; and by enzyme-linked immuno sorbent assay (ELISA) and Biacore analysis, the three antibody proteins have high affinity and high specificity for the fenitrothion, so excellent single-chain antibody genes and antibody proteins are provided for establishing ELISA and immune sensors. The invention has significance for realizing quick, simple, convenient and accurate detection of organic phosphorus pesticide residue, and provides a new direction.

The invention provides a leukemia bacteriophage single-chain antibody library, which uses phasmid pCANTA-5E as a carrier, has storage capacity of 1.5*10<9>, and has excellent diversity in proving of DNA (deoxyribonucleic acid) sequencing. A leukemia cell specific completely humanized single-chain antibody can be obtained conveniently in multiple screenings by using a specific leukemia related antigen as a target through a bacteriophage representation technology from the completely humanized single-chain antibody library. The antibody library provided by the invention has high storage capacity and excellent diversity, and comprises a combination of complete humanized single-chain antibodies; and a single-chain antibody is formed by connection of a heavy chain variable region VH and a light chain variable region VL through a connecting peptide (GGGGS)3, and contains a complete antigen-binding site. The method has the advantages of strong operability and high storage capacity, and can be used for conveniently screening the leukemia cell specific completely humanized single-chain antibody; and the completely humanized single-chain antibody can be used for preparing a target medicament, which is used for treating leukemia.

A controllable siphon drainage device of reservoir drainage and flood discharging of the invention comprises a dam body, a siphon pipe, a gas outlet valve, a gas outlet pipe, a water inlet pipe, a water inlet valve, a gas inlet pipe provided with a valve, a gas-liquid separator, a water charging pipe provided with a valve and a purge valve. The invention is characterized in that: the dam body is provided with a siphon pipe, the entrance end of the siphon is arranged in the lower part inside the reservoir dam body, the exit end of the siphon is provided with an elbow arranged in the lower part outside the reservoir dam, the middle part of the siphon is arranged on the top of the dam body, the siphon pipe is provided with a gas inlet pipe with a valve, the siphon pipe is connected to the gas-liquid separator through the gas outlet pipe and the water inlet pipe, the gas outlet pipe is provided with a gas outlet valve, the water inlet pipe is provided with a water inlet valve, the top end of the gas-liquid separator is provided with a purge valve and a water charging pipe with a valve. The invention has the advantages of simple operations, convenience and quickness, work-saving and labor-saving; can improve the effective height, reservoir capacity, flood charging and discharging and normal drainage ability, thereby improving the reservoir safety and flexibility.

The invention discloses an automatic cylindrical multi-dimension parking garage. A multilayer outer-circle base plate is fixed between an outer frame and an inner frame, and a notch is formed in the inner frame. A rotary table is arranged on a base located at the center of the inner frame, and a center stand column is fixed on the rotary table. One end of a vehicle carrying plate is connected to the center stand column in a sleeving mode and is in up-down sliding fit connection with the center stand column. First telescopic hydraulic rods are fixed on the rotary table and are used for lifting the vehicle carrying plate. Tray guide rails are arranged on the vehicle carrying plate, idler wheels matched with the tray guide rails are arranged at the bottom of a tray base, and a second telescopic hydraulic rod enables the tray base to move along the tray guide rails. A shear fork hydraulic lifting mechanism is fixed between the tray base and a tray to be used for lifting the tray. An outer-circle parking plate is rotatably mounted on the outer-circle base plate, and a plurality of outer-circle partition plates divide the outer-circle parking plate into a plurality of outer-circle parking spots. A plurality of inner-circle partition plates divide an inner-circle parking plate into a plurality of inner-circle parking spots. According to the automatic cylindrical multi-dimension parking garage, garage spots can be increased, the garage storage capacity is improved, the time for parking and taking vehicles is shortened, the vehicle taking efficiency is improved, fully-automatic control is achieved, safety is achieved, and the cost is low.

The invention discloses an automatic gravity type flue-cured tobacco lamina formula warehouse control system. A warehousing stacker roadway for running of a warehousing stacker is arranged between a warehousing system and a gravity type goods shelf, an ex-warehouse stacker roadway for running of an ex-warehouse stacker is arranged between an ex-warehouse feeding system and the gravity type goods shelf, the warehousing system comprises a warehousing conveying line in butt joint with the warehousing stacker, the ex-warehouse feeding system comprises a flue-cured tobacco lamina packet feeding sequence arrangement buffer in butt joint with the ex-warehouse stacker, the flue-cured tobacco lamina packet feeding sequence arrangement buffer is connected with an unpacking machine through a feeding conveying line, the gravity type goods shelf is provided with a plurality of passages with multiple goods allocations, and the warehousing stacker and the ex-warehouse stacker are in butt joint with a goods inlet end and a goods outlet end of each passage respectively. The system combines the advantages of high automation degree of a roadway stacker elevated warehouse mode and high storage capacity of a drive-in racking storage mode, achieves dense storage and automatic warehousing and ex-warehouse of flue-cured tobacco lamina packets, and is less in investment and low in energy consumption.

The invention mainly discloses a vertical circulation type stereo roll storage, which comprises a steel framework, a mechanical drive mechanism and hook assemblies. The mechanical drive mechanism is mounted on the steel framework, and comprises a motor-speed reducer assembly, a driving sprocket assembly, a driven sprocket assembly and drive chains, the driving sprocket assembly is driven by the motor-speed reducer assembly, and the driven sprocket assembly and the driving sprocket assembly are on the same vertical plane, and are connected with each other through the chains. The hook assemblies for hanging rolls are fixedly connected with the drive chains. The invention provides the automatic or semiautomatic storage equipment for storing roll freight, the design of which is reasonable and which is convenient to operate and has a high space utilization rate.

The invention relates to an intensive type storage system which comprises a multi-point type support goods shelf module, a child-mother shuttling trolley module, a child-mother shuttling trolley conveying platform module, a child-mother trolley automatic charging module and an automatic high-stacking module. The multi-point type support goods shelf module is a goods shelf which can store large-scale cargoes through goods shelf support without a support disc. The number of support points of the multi-point type support goods shelf (1) is at least two. One mother trolley (4) and one son trolley (3) or one mother trolley (4) and a plurality of son trolleys (3) are used for conveying one cargo box (2) in a synchronous mode and are matched with the multi-point type support goods shelf module. The support disc is not used, the problem of collapse of a large-size paper box can be solved, storage capacity is increased, operation cost is lowered, a forklift is of no need, operation automation can be achieved, a shuttling trolley can be charged automatically, a charge process is simple, and single-layer cargo shelf storage capacity is maximized.

The invention discloses a rice panicle trait regulatory gene PT2 and application thereof. A nucleotide sequence of the PT2 gene is as shown in SEQ ID NO.1, and the PT2 gene can regulate rice panicle traits such as grain shape, 1000-grain weight and panicle length. The invention further provides allelic genes pt2-J and pt2-I of the PT2 gene. The nucleotide sequences of the allelic genes are as shown in SEQ ID NO.3 and SEQ ID NO.5. The PT2 gene provided by the invention and the allelic genes thereof can be used for breeding new plant varieties such as a high-yield rice variety, or a top-grade long-grain high-grade rice variety, and can also be used for breeding high-yield top-grade novel varieties of other plants such as corns, sorghums, and the like.

The invention discloses a superfine particle tailing reservoir heightening and capacity expanding stacking-discharging structure capable of achieving one-time dam building by adopting a center line method. The structure is formed by combining an original tailing blocking dam (1), a dam abutment flood intercepting trench (12), a first-stage heightening dam body (2), a second-stage heightening dam body (3), a third-stage heightening dam body (4),..., and an nth-stage heightening dam body; a row of medium-coarse tailing drainage blind drains (9) which are parallel to one another and perpendicularto the axis of the corresponding dam body are constructed in the portion on the top layer of each stage of heightening dam body; draining communication pipes (7) and underground water collecting pipes (11) are laid; drainage floral tubes (8) are buried in the medium-coarse sand drainage blind drains (9); and layers of high-strength anti-corrosion geogrid (6) are laid every 0.5-1.0 m of verticalinterval. According to the invention, solidification of fine particle tailings is accelerated, the mechanical strength index of the fine particle tailings is improved and increased, thus, the capacityof a tailing reservoir is remarkably increased, the production service life is prolonged, and meanwhile, the problems that the capacity of parts of waste dumps are not enough, and mining waste rock is stacked are solved.

Belonging to the field of molecular biology, the invention in particular relates to an anti-idiotypic nano antibody based zearalenone green immunoassay method. According to the invention, the zearalenone monoclonal antibody 2D3 is employed to immunize alpaca, and the leukocyte total RNA is extracted to construct a phage displayed nano antibody library; the 2D3 antibody is adopted as the target, four rounds of affinity enrichment panning are conducted to lower the coating concentration and competitive elution concentration round by round, and a positive phage zearalenone anti-idiotypic nano antibody with good specificity can be obtained. The invention employs the zearalenone anti-idiotypic nano antibody substitute zearalenone standard ELISA method to determine naturally polluted corn and feed samples, and performs comparison with the HPLC detection result, the two methods have good correlation, and R2 is 0.997, thus proving that the zearalenone anti-idiotypic nano antibody substitute standard method has accurate and reliable result and is an effective and feasible green immunoassay method.

The invention relates to the technical field of logistics and warehousing, and particularly to a warehousing method and device of intensive warehouses with cargo pallet for storage, warehousing and ex-warehousing. The warehousing device comprises parallel multilayer through storage channels supported by beams and vertical posts, wherein a warehouse entrance and a warehouse exit are arranged at two ends of each through storage channel; an unpowered roller way and an orbit having a certain included angle alpha with a water level are arranged inside each through type storage channel; each orbit is provided with a tablet shuttle car; and a deceleration separation device is arranged at the terminal end of each orbit close to the warehouse exit. The tablet shuttle car is adopted to convey a cargo pallet so as to put the cargo pallet into the warehouse; and a gravity conveying type intensive warehousing way is adopted for warehousing and ex-warehousing. According to the invention, stability and effectiveness of cargo pallet in-warehousing, intensive warehousing and ex-warehousing are guaranteed, cost is lowered, convenience is brought about to the later-period maintenance, and market promotion is facilitated.

The invention discloses a field selection method for ideal plant types of New Indic Rice in the rice areas of the middle and lower reaches of the Yangtze River. According to the ecological characteristics of the growing seasons of the rice in the middle and lower reaches of the Yangtze River, a selection mode and a field selection index are provided for the plant types of one-season Indic rice grains during the yellow maturity period. The key point of the invention is that a selection with three-high: high growing quantity, high storage quantity and high filling degree is established; according to the three-high mode, quantitative selection indexes for the individual and group plants are established. The individual selection indexes are shown as follows: big, erecting, sparse, solid, excellence and resistance; the selection indexes for the group plants are presented as follows; enough, strong, penetration, elegant and standing. When the single plant is selected, the comprehensive property is investigated; meanwhile, the behavior of individual plant in the group is also investigated; when the group plant is selected, the group structure is investigated and the single plant property is investigated at the same time. Individual plants are selected out of excellent group plants and the group plant is established by excellent individual plants; the process is repeatedly promoted to all seed raising generations, thus improving the selection effect. The improvement of single property is developed to the establishment of the comprehensive property; the qualitative analysis of configuration is developed as quantitative selection of the plant factors; the pure configuration design is developed as the improvement of comprehensive function, thus establishing the frame for the comprehensive selection of the ideal plant type of the New Indic Rice.

The present invention relates to a high-yield breeding method of round-grained non-glutinous rice sterile line, belonging to the field of seed production technology. Said high-yield breeding method includes the following measures: defining breeding process, defining breeding field, defining ridge-forming mode of male parentage and female parentage, defining reasonable spacing between rows of male parentage plants and spraying plant growth regulating agent, etc. Said invention can raise setting percentage and can greatly raise yield.

The invention provides a vertical lifting stereo garage, which comprises a garage main body, a moving support, rails, a lifting mechanism, a longitudinal moving mechanism and a transverse moving mechanism. Power is provided for longitudinal moving of the moving support through a wheel hub motor, and the wheel hub motor is mounted at the bottom of the moving support through a motor bracket and comprises a rotor shell, a first end cover, a second end cover, a stator carrier, a stator end cover, a motor stator, a motor spindle, a stator winding, a permanent magnet and a special bearing; the lifting mechanism is constituted by a frameless permanent magnet synchronous motor, a driving wheel, a first guide pulley, a second guide pulley, a horizontal fixed pulley, a pulling fixed pulley, a lifting platform, a counterweight and a pulling rope; and the transverse moving mechanism comprises a vehicle carrying board, a vehicle frame sliding rail, a vehicle frame positioning groove, a lifting platform sliding rail, a first-stage electromagnetic unit, a second-stage electromagnetic unit, a first-stage horizontal moving board and a second-stage horizontal moving board. The space utilization ratecan be increased, the vehicle parking and picking-up efficiency can be improved, thus land resources are saved, and the maintenance and management costs are lowered.

The invention relates to an aisle-stack stereoscopic garage driven by linear motors. The aisle-stack stereoscopic garage comprises a garbage frame, a plurality of parking places, a stacking type movable lifting platform, a lifting platform driving device, transverse moving car loading plates, a passage and passage layer track devices, wherein the parking places are arranged in a stacking way and are arranged along the length direction of the garbage frame in a stretching way, the passage is arranged at the middle part of the garbage frame, the stacking type movable lifting platform is arranged in the passage, the left side and the right side of the passage are respectively provided with a row of parking places, the stacking type movable lifting platform is driven to horizontally move along the length direction of the garbage frame by the lifting platform driving device, the lifting platform driving device comprises a primary linear motor and a secondary linear motor which are mutually and electrically connected in a matching way, the secondary linear motor is fixedly arranged on the top surface in the garbage frame, and the primary linear motor is arranged on the top surface of the stacking type moving lifting platform. The aisle-stack stereoscopic garbage driven by the linear motors, disclosed by the invention, has the advantages that the structure is simple, the installation is convenient, the design is flexible, the cost is low, the garbage capacity is increased by multiple times, the time for parking or taking a car by a car owner in one time is short, and the like.

An interaction type multi-layer garage parking, including garage body, winding engine set and circuit control unit, characterized in: the garage body is made up of over-ground part and under-ground part, and the rail traveled distance of the two parts is equal; in the garage body, many steel rails are arranged, the garage body is separated into two equal spaces, a hanging box is arranged in each space, and multi-layer parking spaces are arranged in each hanging box, on the top of the hanging box, a pulley is arranged, and on each side of the hanging box body, at-least 2 concave formed rollers are mounted, each roller is engaged to a steel rail, controlled by the circuit, the winding engine can implementing parking in dynamic garage and static vehicle state by making one hanging box rising and another falling simultaneously. The falling one conducing to the rising of the other one by interaction. And the control circuit can make the specified parking space stop by the over-ground entrance for convenient in-and-out of vehicles. The garage is suitable for urban residential district and public places.

The invention discloses a novel heightening expanding method for a one-time constructed dam ultra-fine tailings pond through a midline method. The method is characterized in that heightening expandingis carried out through a grading and staging construction method, a first-stage heightening dam body (2), a second-stage heightening dam body (3), a third-stage heightening dam body (4) and the likeare sequentially piled and constructed; a row of medium-coarse sand seepage drainage blind ditches (9) which is parallel among each other and perpendicular to the axis of each dam body are constructedin the top layer of the heightened dam body; water drainage communicating pipes (7) and underground water collecting pipes (11) are laid; seepage drainage floral pipes (8) are buried in the medium-coarse sand seepage drainage blind ditches (9) of the heightening dam bodies; and a layer of high-strength corrosion-resistant geogrid (6) is fully paved at every vertical interval of 0.5-1.0 m. According to the novel method, the consolidation of fine tailings is accelerated, the mechanical strength index of the fine tailings is improved, the storage capacity of the tailings pond is remarkably increased, the production service life is prolonged, the pressure of tailings discharge of enterprise production is relieved, and meanwhile the problems that the capacity of part of dumping sites is insufficient and mining waste rocks are piled up are solved.

Disclosed is an adjustable and recyclable transportation shelf. The adjustable and recyclable transportation shelf comprises a shelf body. The shelf body is of a three-layer structure, which is formed by sequentially stacking base wood cushions, base sleepers and support beams from top to bottom in a staggered mode; on every layer of the shelf body, a plurality of the base wood cushions, the base sleepers or the support beams are arranged at equal intervals; the base wood cushions and the base sleepers are fixed through welding; the top surfaces of the base sleepers are provided with positioning pin holes; both ends of every support beam are provided with positioning pins; through cooperation between the positioning pin holes and the positioning pins, the position of the support beams can be adjusted to adapt to stacking of products of different specifications. Meanwhile, the adjustable and recyclable transportation shelf is of the three-layer structure different from the double-layer structure of a traditional #-shaped wood shelf; the transverse and longitudinal stacking blocks of a stacking machine can be adjusted easily for stacking, the preparation time for stacking adjustment can be reduced, and the stacking efficiency can be improved; by means of multi-layer stacking design, warehouse storage capacity can be effectively increased; steel-structured design can improve hoisting and transportation security. Meanwhile, the adjustable and recyclable transportation shelf is recyclable and reusable and effectively reduces the manufacturing cost.

The invention provides a construction method of a Pichia stipitis large-fragment DNA (deoxyribonucleic acid) genome library, which comprises the following steps: (1) extracting genome DNA of Pichia stipitis of which the collection number is ATCC NO:58376; (2) constructing a recombinant vector; (3) screening a positive clone; and (4) performing enzyme cutting identification on the positive clone, and saving a library. The invention successfully constructs the Pichia stipitis large-fragment DNA genome library for the first time; the storage capacity of the library is high (the cloning probability is 97%), and the coverage rate is high (N=1.08); and a gene related to cellobiase can be successfully screened by using the genome library, thereby laying a foundation for the screening of an unknown functional target gene, the functional research on the related gene and the further reconstruction on the gene level so as to obtain an ideal industrially-produced strain.

The invention discloses a liver cancer microsphere cell surface specific targeting polypeptide. The polypeptide is a heptapeptide, and the amino acid sequence of the polypeptide is Asn-Thr-Gly-Ser-Pro-Tyr-Glu. A preparing method and applications of the polypeptide are also disclosed. The polypeptide can be specifically bound with liver cancer microsphere cells, has high targeting binding capacity for liver cancer microspheres, high affinity and high specificity, and has no influence on cell growth. According to the method, liver cancer microspheres subjected to HCCLM3 cell suspension culture are adopted as target cells, HCCLM3 cells and LO2 cells subjected to adherent culture are adopted as adsorption cells, and subtractive screening is performed by utilizing a phage display technology. In the method, storage capacity is high, the screening success rate is high, operation is simple and the cost is low. The screened polypeptide can be used for preparing liver cancer embolus diagnostic reagents or liver cancer targeting treatment medicines.

The invention relates to the field of molecular biology, and in particular discloses a zizania latifolia complementary deoxyribonucleic acid (cDNA) library construction method. The zizania latifolia cDNA library construction method provided by the invention takes tender leaves of zizania latifolia as a test material, and comprises the twelve steps of ribonucleic acid (RNA) extraction, cDNA first strand synthesis, long-distance polymerase chain reaction (LD PCR), proteinase K digestion, cDNA library homogenization, twice PCR amplification, amplified product purification, Sfi I digestion, enzyme digestion cDNA purification, connection of cDNA and a PUC19 carrier, transformation of plasmid, cloning identification, and the like. The method is convenient and fast in library construction; the constructed library is high in capacity, high in recombination rate and good in integrity; after homogenization, high-abundance genes are obviously reduced, so that a foundation is laid for the verification of functional genes. The zizania latifolia cDNA library constructed by the method is high in capacity, and the capacity reaches 3.6*10<6>pfu.mL<-1>; the average length of an inserted fragment is 1000bp, and the recombination rate is 97.9%; the normalized full-length cDNA library can effectively enrich low-abundance expression genes, thus reducing a redundancy rate. The zizania latifolia cDNA library is used for screening a target gene and for subsequent gene and protein interaction analysis.

The invention relates to a sediment storage dam covered with geogrid reinforced cement modified soil and a construction method of the sediment storage dam. A dam body of the sediment storage dam comprises a filling core formed by filling and compacting granite residual soil and a cover layer for wrapping the filling core, and the cover layer is formed by superposing cement modified soil wrapped by geogrids. The cement modified granite residual soil is used as a main building material, and a corresponding damming construction method is combined, so that the construction speed is increased, meanwhile, the construction cost and difficulty of the sediment storage dam are greatly reduced, and the sediment storage dam covered with the geogrid reinforced cement modified soil has the remarkable advantages such as simplicity in construction, high construction speed, low cost and high scour resistance and is particularly suitable for controlling granite collapse.

The invention relates to the technical field of fertilizers, in particular to an anti-drought fertilizer special for wheat and a use method thereof, and aims to provide a special fertilizer that is specific for dry areas in north China, can improve the drought and lodging resistant capability of wheat, reduce pest and disease damage, and finally ensure wheat yield. Foliar-fertilizers choline chloride and calcium hydrogen superphosphate are sprayed to improve photosynthesis and promote dry matter accumulation, so that distribution of dry matters into sink organ is in a coordinated source-sink relationship, the distribution proportion of a photosynthetic product into a sink organ increases, the retention amount of nonstructural carbohydrate in stems and leaves decreases, thus actively improving the sink capacity and promoting movement of a lot of source leaf photosynthetic products into grains.

The invention belongs to the field of molecular biology, and particularly relates to an aflatoxin M1 anti-idiotype nano antibody and a preparation method thereof. An amino acid sequence of the aflatoxin M1 anti-idiotype nano antibody is as shown in SEQ ID NO:1. According to the invention, alpaca is immunized by an aflatoxin M1 monoclonal antibody, a phage display nano antibody library is constructed by extracting total RNA of leukocytes of the alpaca, and the library is high in capacity and good in diversity; the aflatoxin M1 monoclonal antibody is used as a target object, four rounds of affinity enrichment panning are adopted, the coating concentration and the competitive elution concentration are reduced round by round, and a positive bacteriophage aflatoxin M1 anti-idiotype nano antibody with good specificity is obtained through panning; and a positive bacteriophage aflatoxin M1 anti-idiotype nano antibody gene is transformed into an expression strain, and a protein-form nano antibody VHH C4 with excellent performance is obtained through induced expression, purification and identification.

The present invention discloses one kind of automatic file information searching and checking system. Rotary file cabinets are used as basic storing facility, normalized file boxes with electronic or electromagnetic marker, such as bar code, IC card, magnetic card, etc. are used, and checking identifier, entrance and exit hand-held identifier, synchronous moving display and file management computer are provided, to realize the automatic searching, positioning and checking.

The invention discloses a construction method and an application of an anti-PEDV (Porcine Epidemic Diarrhea Virus) N protein VHH (variable region of heavy chain) bacteriophage display library. The construction method comprises the following steps: carrying out extraction and reverse transcription of immune two-humped camel peripheral blood lymphocyte and lymphocyte total RNA (Ribonucleic Acid); designing two pairs of PCR (Polymerase Chain Reaction) amplification primers to obtain a target fragment; connecting the obtained target fragment with a phagemid carrier pCANTAB-5E; converting a connection product into a TG1 competent cell, and obtaining the bacteriophage display library; successfully constructing the VHH bacteriophage display library of the anti-PEDV N protein; calculating the capacity and the diversity of the library; and carrying out quality evaluation on the constructed library. The library established with the construction method has the characteristics of large library capacity, rich diversity and the like, and lays a foundation for establishing a clinic diagnosis method of the PEDV and researching a virus infection mechanism.

The invention discloses a method for heightening and expanding the capacity of an ultra-fine tailings reservoir for one-time construction of the dam by adopting the center line method, adopting a staged construction method for heightening and expanding the capacity, and successively stacking the first level of heightening dam body (2), The second-level heightening dam body (3), the third-level heightening dam body (4) ..., construct a row of medium-coarse sand seepage drainage of the level parallel to each other and perpendicular to the axis of the dam body on the top floor of each level of heightening dam body For the blind ditch (9), lay drainage connecting pipes (7) and groundwater collection pipes (11), and bury seepage flower pipes (8) in the medium-coarse sand drainage blind ditch (9) of this level, with a vertical interval of 0.5m~ A layer of high-strength corrosion-resistant geogrid (6) is fully paved at a height of 1.0m. The method of the invention accelerates the consolidation of fine-grained tailings, improves and increases the mechanical strength index of fine-grained tailings, significantly increases the storage capacity of tailings storage, prolongs the service life of production, and relieves the pressure of enterprises to discharge tailings. , At the same time, it also solves the problem of insufficient capacity of some dumps and the accumulation of mining waste rocks.