369results about "Protein nucleotide libraries" patented technology

The disclosure relates to compositions and methods for the preparation, use, and / or formulation of adeno-associated virus capsid protein variants.

The disclosure relates to compositions, methods, and processes for the preparation, use, and / or formulation of adeno-associated virus capsid proteins, wherein the capsid proteins comprise targeting peptide inserts for enhanced tropism to a target tissue.

The present invention relates to methods of detecting region(s) of interest in a gene comprising a polyA tail. The region(s) of interest can include gene(s), region(s), mutation(s), deletion(s), insertion(s), indel(s), and / or translocation(s). The region(s) can be greater than or less than 1 kilobases from the polyA tail. Methods can include forming a library of single cell transcripts comprising the region(s) in close proximity to a cell barcode and a unique molecular identifier (UMI). Methods for distinguishing cells by genotype can include amplifying the transcripts using PCR methods and detecting the cell barcode and UMI using single cell sequencing methods. Transcripts can be enriched using tagged region-specific PCR primers. Cell barcodes can be brought into close proximity to the region(s) by circularizing the transcripts. Sequencing of the transcripts can include using primer binding sites added during PCR amplification and library indexes for multiplexed sequencing.

The present invention provides an hBCMA-binding polypeptide comprising at least one motif that binds to hBCMA, wherein the peptide has the following structure: [N-terminal portion]-[Helix 1]-[Spacer portion]-[Helix 2]-[C-terminal portion], and the hBCMA-binding motif is the portion [Helix 1]-[Spacer portion]-[Helix 2]. The present invention further provides a pharmaceutical composition comprising the hBCMA-binding polypeptide, and the use of the hBCMA-binding polypeptide or the pharmaceutical composition for use as a medicament, particularly for the treatment or prevention of cancer.

This invention relates to a method for designing and utilizing a broad RNA modification library that includes all surrounding sequences of the RNA modification. By introducing multiple types of RNA modifications, this invention enables the construction of RNA modification libraries that encompass all possible combinations of surrounding sequences flanking the modified RNA. Therefore, it differs from existing reported methods for constructing RNA modification libraries. Furthermore, using the RNA modification library of this invention for single-molecule RNA sequencing generates a high-quality reference dataset containing all possible combinatorial motifs. If this dataset is used as training data for deep learning (or machine learning), it overcomes the limitations of existing software that is limited to specific RNA modifications or has low prediction accuracy for other modifications. This allows for the development of novel, more accurate RNA modification detection software and the simultaneous detection of multiple RNA modifications.

To provide an amino acid capable of improving the membrane permeability of peptide compounds, as well as a peptide compound including the amino acid.SOLUTION: The present invention provides an amino acid having a side chain capable of forming at least one intramolecular hydrogen bond.SELECTED DRAWING: None

The present invention provides systems and methods for cell free RNA sequencing. Targeted sequencing can be performed using a set of gene transcripts that are rare abundance in a population of cell free nucleic acid control samples.

The present invention aims to provide an immunoglobulin-binding protein having an improved chemical stability, especially having an improved alkaline stability, or an immunoglobulin-binding protein capable of allowing antibody elution under mild pH conditions, and an adsorbent on which the protein is immobilized. The object is achieved by improving the alkaline stability or the antibody elution property by including an amino acid sequence which is the same as an amino acid sequence of an immunoglobulin-binding domain of protein L derived from a bacterium belonging to the genus Finegoldia except that the amino acid residue(s) at a particular position(s) of the domain in the amino acid sequence is / are substituted with another particular amino acid residues(s).

The present disclosure provides a recombinant Salmonella strain having a Type III secretion system (T3SS) comprising mutation which enhance protein expression and production. Additionally, methods and kits for using the recombinant Salmonella strain for producing a protein of interest are provided. Additionally, an optimized medium that increases protein expression in a Salmonella strain having a Type III secretion system (T3SS) is provided.

The present invention provides a method for constructing a single-cell Hi-C library, comprising the steps of crosslinking DNA and proteins within sample cells, enzymatically cleaving the DNA in the crosslinks, adding sequencing adapters, religating the DNA fragments, isolating single cells, resolving crosslinks, adding library adapters with cell barcodes, and amplifying the library. The single-cell Hi-C library constructed using this method has a short construction time, low cost, convenient experimental operation, and a high percentage of valid data.

Systems and methods for protein engineering. The systems include a sequence testing subsystem and a machine learning subsystem. The sequence testing subsystem is configured to express proteins and test the expressed proteins for a given property. The machine learning subsystem is configured to model the activities of a set of possible proteins in light of the properties of the tested proteins and provide one or more untested proteins in the set to the sequence testing subsystem for subsequent testing. The system can be run in an iterative fashion and be fully automated. Methods of using the systems are provided.

Provided herein are antigen binding proteins that bind to thyroid stimulating hormone receptor (TSHR) and methods of using the same.

The present invention includes compositions and methods useful for the detection of a mutational change, SNP, translocation, inversion, deletion, change in copy number, or other genetic variation within a sample of cellular genomic DNA or cell-free DNA (cfDNA). In some embodiments, the compositions and methods of the present invention provide an extremely high level of resolution that is particularly useful in detecting copy number variations in a small fraction of the total cfDNA from a biological sample (e.g., blood).

The present invention relates to a method for producing a nucleic acid library, the method comprising (1) a step for providing a first library that is composed of mutants produced by randomly introducing mutations in a nucleotide sequence encoding a protein capable of being bound to a target or a protein intended to be bound to the target by a phage display method, (2) a step for subjecting the first library to biopanning to produce a sublibrary and then acquiring data to be used in a machine learning, and (3) a step for performing the machine learning using the data to acquire a second library from the first library on the basis of the machine learning prediction, in which the data used in the machine learning include the sequences of a mutant mass included in the sublibrary in the target-binding sequence elution procedure stage, the estimated strength of binding to the target, and actually measured values for the binding of one or some of the mutants included in the mutant mass to the target.

The present invention provides a simple and inexpensive system for regulating signal peptide performance by using a synthetically evolved nucleotide sequence. The invention further relates to an expression vector comprising the nucleotide sequence. Additionally, the present invention relates to host cell comprising the expression vector. Furthermore, the present invention relates to a recombinant protein expressed by the host cell as well as a method for expressing the recombinant protein.

The invention discloses DNA (deoxyribonucleic acid) coupled non-natural amino acid as well as a synthesis method and application thereof. The synthesis method of the DNA coupled non-natural amino acid comprises the following steps: adding inorganic alkali and 2, 5-dibromohexane diamide into a boric acid buffer solution containing DNA coupled cysteine as shown in a general formula (I) in the specification, adding an organic phase, and reacting at 25-37 DEG C to enable the DNA coupled cysteine to be subjected to desulfurization elimination, thereby obtaining the DNA coupled non-natural amino acid. DNA coupling dehydroalanine shown as a general formula (II) in the specification is obtained; the method comprises the following steps: carrying out nucleophilic addition reaction on DNA coupled dehydroalanine and a nucleophilic reagent under an alkaline condition or carrying out dipolar cycloaddition reaction on DNA coupled dehydroalanine and 2, 5-substituted tetrazole under an ultraviolet condition to obtain the DNA coupled unnatural amino acid. By adopting the method, the integrity of the DNA chain can be effectively ensured, no by-product is generated in the reaction, and a more powerful chemical tool is provided for construction and subsequent modification of a DNA coding molecular library.

The application provides a Hi-C library construction method, which comprises the following steps: cross-linking DNA and proteins in sample cells, enzyme cutting the DNA in the cross-linking body, adding a sequencing adaptor, re-connecting the DNA fragments, de-cross-linking, adding a library adaptor, and library amplification. By using the Hi-C library construction method of the application, a Hi-C library with short library construction time, low cost, convenient experimental operation and high effective data proportion can be realized.

Provided are a microorganism comprising variant LysE, and an L-amino acid producing method using same. The variant LysE may improve L-amino acid excretion and / or production capacity compared to a wild type.

The present disclosure provides methods, compositions, and systems for evolving virus-like particles (VLPs) having one or more desired properties such as increased production levels, increased cargo packaging efficiency, and / or increased transduction of particular target cell types of interest. The present disclosure also provides libraries for use in such methods, and methods for producing the libraries. Group specific antigen (gag) proteins comprising nucleocapsid protein variants evolved using the methods described herein are also provided herein. The present disclosure also provides VLPs comprising such gag proteins comprising nucleocapsid protein variants. Polynucleotides, vectors, cells, and kits useful for performing the methods described herein are also provided.

The present invention provides cell vaccine platforms, such as vaccine-immune virus opsonization platforms, for inducing a host immune response, as well as methods for producing and using such cell vaccine platforms. [Solution] Several embodiments provide genetically engineered human cells comprising (a) genomic disruption in at least one human leukocyte antigen (HLA) gene or at least one transcription regulator of an HLA gene, and (b) an exogenous nucleic acid encoding a cell surface protein that binds to a protein expressed on the surface of phagocytic or cytolytic immune cells, or a functional fragment or functional variant of the cell surface protein, wherein the binding results in activation of the phagocytic or cytolytic activity of the immune cells.

The present disclosure relates to a novel YhhS variant and a method for producing O-phosphoserine, cysteine and a derivative of cysteine using the same.

Described herein are VHH antibody libraries with heavy chain variable domain framework scaffolds that have complementarity determining regions (CDRs) found in naturally occurring human antibodies, and methods for making such antibody libraries. The antibody libraries do not include members that contain one or more liabilities that affect one or more properties of the members.

The present disclosure relates to methods of producing and screening libraries of disulfide rich proteins, for instance to identify binding peptides specific for a target molecule. In some embodiments, the binding peptides comprise disulfide rich proteins. Also provided herein are methods of producing and screening libraries comprising disulfide-rich proteins and molecular chaperones. The present disclosure also relates to methods of producing or expressing soluble disulfide rich proteins, for instance using a suitable host cell. Also provided herein are compositions comprising soluble disulfide rich proteins.

Use of a compound, microarray chip substrate, microarray chip and use and method thereof. The present application provides use of a compound, or a geometric isomer, tautomer, isotopically labeled, hydrate, solvate or salt thereof in chip surface modification. Also provided is a microarray chip substrate modified by the compound, and a microarray chip comprising the microarray chip substrate. The microarray chip substrate of the present application can greatly improve the surface modification function by surface modification, improve nucleic acid synthesis yield, amplify detection signal, and avoid non-specificity and cross-talk. It has a wide application prospect.

A method for generating a plurality of neoantigens from a sample obtained from a patient, comprising the steps of: - sequencing the DNA and / or RNA from the sample; - identifying a number of DNA and / or RNA variants from the sequencing data; - identifying from said plurality of DNA and / or RNA variants those which give rise to qualitative differences in the corresponding encoded peptides and / or those which give rise to the generation of novel peptide sequences; and generating said plurality of neoantigens, wherein each neoantigen contains one of said identified differences in said encoded peptide.

The present invention provides a method for constructing a cyclic compound library that overcomes the limitations of conventional ring-closing methods for cyclic compound libraries, has the advantages of milder ring-closing reaction conditions and high generality, can be used to construct monocyclic and bicyclic compound libraries, and involves few side reactions. [Solution] A cyclic compound library and its construction method are provided, which uses a solid support, a molecule containing a photocleavable group, a linker, a building block, a ring-closing A-terminal molecule, and a ring-closing B-terminal molecule at both ends of the reaction synthesis, and utilizes the decomposition of the solid support under light irradiation to complete ring closure of the amino acid residue structures of the ring-closing A-terminal molecule A and the ring-closing B-terminal molecule B through the action of cyclohydrolase. This method, which uses mild ring closure conditions, is more universal and expands the types of chemical reactions and the diversity of the encoded compound library.

A library preparation device is a chip including (a) a biological sample well, (b) an end repair and elution well, downstream from the biological sample well, (c) an adapter ligation and elution well, downstream from the end repair and elution well, and (d) a final library well downstream from the ligation and elution well. The library preparation device is useful in a method of library preparation on a library preparation device.

Provided herein are biparatopic antigen-binding constructs that specifically bind HER2. The biparatopic antigen-binding constructs comprise one antigen-binding moiety that binds to ECD2 of HER2, a second antigen-binding moiety that binds to ECD4 of HER2, and an Fc. At least one of the antigen-binding moieties is an scFv. The biparatopic antigen-binding constructs can be used in the treatment of cancer.