537results about "Protein nucleotide libraries" patented technology

The disclosure relates to compositions and methods for the preparation, use, and / or formulation of adeno-associated virus capsid protein variants.

The disclosure relates to compositions and methods for the preparation, use, and / or formulation of adeno-associated virus capsid protein variants.

The disclosure relates to compositions and methods for the preparation, use, and / or formulation of adeno-associated virus capsid protein variants.

The invention provides systems, methods, reagents, apparatuses, vectors, and host cells for the continuous evolution of nucleic acids. For example, a lagoon is provided in which a population of viral vectors comprising a gene of interest replicates in a stream of host cells, wherein the viral vectors lack a gene encoding a protein required for the generation of infectious viral particles, and wherein that gene is expressed in the host cells under the control of a conditional promoter, the activity of which depends on a function of the gene of interest to be evolved. Some aspects of this invention provide evolved products obtained from continuous evolution procedures described herein. Kits containing materials for continuous evolution are also provided.

The disclosure relates to compositions and methods for the preparation, use, and / or formulation of adeno-associated virus capsid protein variants.

The invention provides a simplified genome sequencing library construction method and a kit and related application thereof. The construction method of the simplified genome sequencing library comprises the following steps: (1) respectively carrying out enzyme digestion on a plurality of DNA (Deoxyribonucleic Acid) samples by using restriction endonuclease; wherein restriction endonucleases adopted by all the samples are different from one another, and the tail ends of specific DNA fragments of the samples are generated to serve as sample bar codes of the samples; (2) mixing the enzyme digestion products of the samples, constructing a DNA library, carrying out fragment sorting on the DNA library, and taking the selected library as a simplified genome sequencing library; or carrying out fragment sorting on the enzyme digestion product of each sample, and carrying out DNA library construction by using the selected fragments to obtain the simplified genome sequencing library. According to the method, the experimental operation process is simplified, the library building efficiency is improved, and meanwhile, the feasibility of the restriction endonuclease for recognizing 6 basic groups based on a third-generation long-read-long sequencing platform in the simplified genome sequencing technology is also explored.

The disclosure relates to compositions, methods, and processes for the preparation, use, and / or formulation of adeno-associated virus capsid proteins, wherein the capsid proteins comprise targeting peptide inserts for enhanced tropism to a target tissue.

The present invention relates to compositions and methods related to antibodies or antibody fragments that specifically bind to CD38. The composition comprises an antibody or antibody fragment, wherein the antibody or antibody fragment comprises, as a component thereof, one or more complementarity determining regions or framework regions of a light chain variable region or a heavy chain variable region that comprises an amino acid sequence selected from the group consisting of predetermined amino acid sequences.

The embodiment of the invention provides a library construction method, a universal joint and a kit, relates to the technical field of synthetic biology, and is used for constructing a mixed library in which multiple gene segments are directionally connected. The library construction method comprises the following steps: providing a plurality of first initial gene segments; introducing cutting sequences to two ends of each first initial gene segment to obtain a plurality of second initial gene segments; introducing universal joints to two ends of each second initial gene segment to obtain a plurality of target gene segments; wherein the universal joint comprises an identification sequence; performing cutting and connection reaction on the plurality of target gene segments to obtain a mixed library; wherein the plurality of target gene segments are connected according to a set sequence. The library construction method is used for constructing the DNA mixed library.

The present disclosure relates to a method of screening for and producing an mRNA sequence with improved intracellular stability that can exist in cells for a long period of time. According to a method according to an aspect, an mRNA sequence with improved intracellular stability can be screened for and produced by introducing a variation into a nucleotide sequence encoding a target protein such that there is no change in an amino acid sequence of the target protein. When the method is used, there is the advantage of improving the expression level of the target protein in vivo.

This disclosure provides a powerful screening platform that combines pre-templated instant partitions with DNA-encoded library (DEL) technologies to identify target small molecule interactions and analyze their intracellular effects in single cell resolution using methods that require minimal sample preparation and affordable sequencing costs. These methods are well suited for rapidly evaluating drug candidates by simultaneously detecting novel target-binding molecules while screening their transcriptional effects with a single affordable, high throughput workflow.

The present invention relates to methods of detecting region(s) of interest in a gene comprising a polyA tail. The region(s) of interest can include gene(s), region(s), mutation(s), deletion(s), insertion(s), indel(s), and / or translocation(s). The region(s) can be greater than or less than 1 kilobases from the polyA tail. Methods can include forming a library of single cell transcripts comprising the region(s) in close proximity to a cell barcode and a unique molecular identifier (UMI). Methods for distinguishing cells by genotype can include amplifying the transcripts using PCR methods and detecting the cell barcode and UMI using single cell sequencing methods. Transcripts can be enriched using tagged region-specific PCR primers. Cell barcodes can be brought into close proximity to the region(s) by circularizing the transcripts. Sequencing of the transcripts can include using primer binding sites added during PCR amplification and library indexes for multiplexed sequencing.

The present invention provides an hBCMA-binding polypeptide comprising at least one motif that binds to hBCMA, wherein the peptide has the following structure: [N-terminal portion]-[Helix 1]-[Spacer portion]-[Helix 2]-[C-terminal portion], and the hBCMA-binding motif is the portion [Helix 1]-[Spacer portion]-[Helix 2]. The present invention further provides a pharmaceutical composition comprising the hBCMA-binding polypeptide, and the use of the hBCMA-binding polypeptide or the pharmaceutical composition for use as a medicament, particularly for the treatment or prevention of cancer.

The present invention relates to a bicyclic peptide complex comprising a peptide construct, the construct comprising: (i) a polypeptide having a free terminus (N or C); (ii) optionally a nucleic acid encoding the polypeptide; and (iii) a dyad-symmetric linker (TSL) compound attached to the polypeptide, wherein the linker is attached to the terminus of the polypeptide and to at least two distinct side chains of the peptide via a covalent bond. The present invention also relates to a library and methods for making the complex, and to screening methods using the same.

There is provided a method for optimizing a nucleic acid sequence, including the steps of: preparing a nucleic acid sequence that includes a candidate sequence containing a sequence of an untranslated region and a sequence encoding a target protein, and a sequence encoding a peptide barcode directly or indirectly linked to the target protein; expressing a protein from the nucleic acid sequence; separating the peptide barcode from the protein; analyzing the separated peptide barcode; and acquiring a relationship between expression of the target protein and the candidate sequence based on a result of the analysis, and selecting an optimal candidate sequence.

The present disclosure provides compositions and methods for high-throughput screening, selection, and identification of antibodies or antibody-like molecules that bind to target endogenous membrane proteins. TIFF2025524372000023.tif138159

This invention relates to a method for designing and utilizing a broad RNA modification library that includes all surrounding sequences of the RNA modification. By introducing multiple types of RNA modifications, this invention enables the construction of RNA modification libraries that encompass all possible combinations of surrounding sequences flanking the modified RNA. Therefore, it differs from existing reported methods for constructing RNA modification libraries. Furthermore, using the RNA modification library of this invention for single-molecule RNA sequencing generates a high-quality reference dataset containing all possible combinatorial motifs. If this dataset is used as training data for deep learning (or machine learning), it overcomes the limitations of existing software that is limited to specific RNA modifications or has low prediction accuracy for other modifications. This allows for the development of novel, more accurate RNA modification detection software and the simultaneous detection of multiple RNA modifications.

To provide an amino acid capable of improving the membrane permeability of peptide compounds, as well as a peptide compound including the amino acid.SOLUTION: The present invention provides an amino acid having a side chain capable of forming at least one intramolecular hydrogen bond.SELECTED DRAWING: None

Anti-B7-H3 compounds includes an anti-B7-H3 Gp2-based scaffold. The anti-B7-H3 compounds may include one or more functional components such as a targeting component, an imaging component, an enzyme, or a small molecule drug. Compositions including an anti-B7-H3 may be administered to a subject. The subject may have or be suspected of having a tumor or cancer.

The present invention provides systems and methods for cell free RNA sequencing. Targeted sequencing can be performed using a set of gene transcripts that are rare abundance in a population of cell free nucleic acid control samples.

The present invention aims to provide an immunoglobulin-binding protein having an improved chemical stability, especially having an improved alkaline stability, or an immunoglobulin-binding protein capable of allowing antibody elution under mild pH conditions, and an adsorbent on which the protein is immobilized. The object is achieved by improving the alkaline stability or the antibody elution property by including an amino acid sequence which is the same as an amino acid sequence of an immunoglobulin-binding domain of protein L derived from a bacterium belonging to the genus Finegoldia except that the amino acid residue(s) at a particular position(s) of the domain in the amino acid sequence is / are substituted with another particular amino acid residues(s).

The present disclosure provides a recombinant Salmonella strain having a Type III secretion system (T3SS) comprising mutation which enhance protein expression and production. Additionally, methods and kits for using the recombinant Salmonella strain for producing a protein of interest are provided. Additionally, an optimized medium that increases protein expression in a Salmonella strain having a Type III secretion system (T3SS) is provided.

The present invention provides a method for constructing a single-cell Hi-C library, comprising the steps of crosslinking DNA and proteins within sample cells, enzymatically cleaving the DNA in the crosslinks, adding sequencing adapters, religating the DNA fragments, isolating single cells, resolving crosslinks, adding library adapters with cell barcodes, and amplifying the library. The single-cell Hi-C library constructed using this method has a short construction time, low cost, convenient experimental operation, and a high percentage of valid data.

The present invention belongs to the technical field of molecular markers, and particularly relates to a method for identifying rubber tree AB-type cultivated varieties based on chloroplast SNP markers of rubber trees. The SNP locus of the present invention is located at the 147th position from the 5' end of the sequence shown in SEQ ID No.1, and there is a natural variation of C / A at this locus. The SNP locus of the present invention can be used for breeding high-yield rubber tree varieties, expanding the molecular marker library for breeding excellent rubber tree varieties.

A thermostable DNA polymerase mutant with high amplification activity. Using protein directed evolution technology, a random mutation library was constructed for the polymerase active domain targeting Taq enzyme. By gradually adding screening pressure, unsuitable mutations were naturally eliminated, and mutations with advantageous traits gradually accumulated. Finally, a series of amino acid sites and their mutations that play a key role in the amplification performance and polymerization performance of Taq enzyme were screened out, and a Taq enzyme mutant with high amplification performance was obtained.

Systems and methods for protein engineering. The systems include a sequence testing subsystem and a machine learning subsystem. The sequence testing subsystem is configured to express proteins and test the expressed proteins for a given property. The machine learning subsystem is configured to model the activities of a set of possible proteins in light of the properties of the tested proteins and provide one or more untested proteins in the set to the sequence testing subsystem for subsequent testing. The system can be run in an iterative fashion and be fully automated. Methods of using the systems are provided.

Provided herein are antigen binding proteins that bind to thyroid stimulating hormone receptor (TSHR) and methods of using the same.