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Rice panicle trait regulatory gene PT2 and application thereof

A technology for regulating genes and PT2, applied in application, genetic engineering, plant gene improvement and other directions, can solve the problem of complex gene regulation network, and achieve the effect of increasing library capacity and improving quality

Active Publication Date: 2014-06-25
HUNAN HYBRID RICE RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] According to the current research results, the gene regulatory network of rice panicle traits is relatively complex: there are major regulatory genes, and there are also a large number of minor QTLs; pleiotropic genes, there are also genes that only affect a single or a few traits

Method used

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  • Rice panicle trait regulatory gene PT2 and application thereof
  • Rice panicle trait regulatory gene PT2 and application thereof
  • Rice panicle trait regulatory gene PT2 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1 Fine mapping, phenotypic analysis and naming of genes

[0035] After in-depth research, the inventors succeeded in fine-tuning a new major gene GS2 (Grain Shape2) that controls grain length, grain width and thousand-grain weight to the 33.2kb interval on the second chromosome of rice. It is speculated that LOC_Os02g47280 is the candidate gene GS2 (Zhang et al., 2013). This gene can increase rice grain length by 3mm, grain width by 0.4mm, and 1000-grain weight by 18g (the increase may vary under different genetic backgrounds). Subsequent studies have shown that this gene can not only significantly increase grain length, grain width and thousand-grain weight, but also significantly increase ear length (Table 1). The inventor renamed it PT2 (Panicle Traits2). The PT2 gene can simultaneously regulate the panicle length and 1000-grain weight of rice, which has an important impact on yield and quality.

[0036] Table 1 Test results of ear traits of near-isogenic line m...

Embodiment 2

[0039] Example 2 Real-Time PCR expression analysis of LOC_Os02g47280 gene

[0040] Real-Time PCR expression analysis found that the LOC_Os02g47280 gene is expressed in various organs during the whole growth period, but the expression level is higher in young panicles. At this time, it is the critical period of young panicle growth and development, and it mainly affects rice panicle traits. The same type. The expression of large-grain material R642-2 (PT2PT2) was significantly higher than that of medium-grain material R642-1 (pt2pt2) ( figure 1 ), it is predicted that the difference in gene expression of LOC_Os02g47280 leads to the difference in gene function and phenotype.

Embodiment 3

[0041] Example 3 LOC_Os02g47280 function prediction

[0042] According to Interproscan (http: / / www.ebi.ac.uk / Interproscan / ) to predict the protein structure, the protein encoded by the LOC_Os02g47280 gene is composed of 394 amino acids and contains two conserved domains of WRC and QLQ. These two structures The domains are related to DNA binding and protein-protein interactions (Kim et al., 2003, plant J. 36:94-104). Using BLASTp to search the protein structure composed of 394 amino acids encoded by the LOC_Os02g47280 gene, it was found that the gene encodes a growth regulator protein, which belongs to the GRF protein family. Previous studies have shown that this protein family is involved in the growth regulation of rice stems and seed development, and the growth of Arabidopsis cotyledons and leaves (Knaap et al., 2000, plant physiol. 122:695-704; Kim et al., 2003, plant J.36:94-104; Ye et al., 2004, plant J.38:348-357).

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Abstract

The invention discloses a rice panicle trait regulatory gene PT2 and application thereof. A nucleotide sequence of the PT2 gene is as shown in SEQ ID NO.1, and the PT2 gene can regulate rice panicle traits such as grain shape, 1000-grain weight and panicle length. The invention further provides allelic genes pt2-J and pt2-I of the PT2 gene. The nucleotide sequences of the allelic genes are as shown in SEQ ID NO.3 and SEQ ID NO.5. The PT2 gene provided by the invention and the allelic genes thereof can be used for breeding new plant varieties such as a high-yield rice variety, or a top-grade long-grain high-grade rice variety, and can also be used for breeding high-yield top-grade novel varieties of other plants such as corns, sorghums, and the like.

Description

Technical field [0001] The invention belongs to the field of plant genetic engineering, and specifically relates to a rice ear trait regulating gene PT2, and also relates to the alleles of the gene and the application of the gene and its alleles in plant breeding such as rice and corn. Background technique [0002] Rice is one of the most important food crops in the world. More than half of the world's population feed on rice as the staple food. The yield and quality of rice are related to the world's food security. The panicle traits of rice include panicle length, number of primary branches, number of secondary branches, grain shape, number of grains per panicle, etc., which have an important impact on the yield and quality of rice. [0003] Take rice panicle traits-grain shape as an example. The grain shape includes grain length, grain width, and grain thickness. It not only directly affects the important component of rice yield-thousand-grain weight, but also affects the appear...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82A01H5/00C12Q1/68A01H5/10
CPCA01H5/10C07K14/415C12N15/8261Y02A40/146A01H6/4636
Inventor 邓华凤张武汉孙平勇舒服何强刑俊杰王杰
Owner HUNAN HYBRID RICE RES CENT
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