13 results about "Blood lymphocyte" patented technology

The application discloses an application of baicalin in treating coronavirus infectious diseases. The new application of baicalin is verified through in-vitro cell tests and mouse tests infected with human coronavirus HCoV-229E, the technical scheme is novel, and a curative effect test of baicalin in treating pneumonia caused by HCoV-229E virus infection is carried out, and the results show that baicalin has a certain inhibitory effect on HCoV-229E infected cells in vitro; baicalin shows good drug efficacy in lung index and inhibition rate of pneumonia mice infected with HCoV-229E virus, can reduce the virus load of lung tissue of pneumonia mice infected with HCoV-229E virus, increase the proportion of peripheral blood lymphocytes of mice, and reduce the content of IL-2, IL-10, TNF-alpha and IFN-beta in lung tissue of mice.

Provided herein are methods of making tumor reactive peripheral blood lymphocytes (trPBLs) using PBMCs obtained from a patient. The method comprises enriching trPBLs from PBMCs using markers that are expressed or secreted by the trPBLs, such as 4- IBB, CD103, CXCL13, IFN-y, CXCR6, CD200, HLA-DR, CD69, PD-1, CD39, CD38, CD49a, etc. Further provided are use of the trPBLs or pharmaceutical compositions thereof for the treatment of cancers.

The invention provides a method for detecting subtype pSTAT4 and Ki67 signals of peripheral blood T lymphocytes of machin through flow cytometry, and belongs to the technical field of biological detection. According to the method, the integrated analysis of the multi-dimensional functional state of the cynomolgus monkey T lymphocyte is realized, key signal channel activation and proliferation information of different subgroup cells can be synchronously obtained through a single experiment, and the detection comprehensiveness and efficiency are greatly improved. According to the method, an accurate tool capable of directly evaluating the response intensity and proliferation potential of T cells is provided for immune mechanism research based on a machin model, particularly immune therapy or disease research related to regulation and control of pathways such as IL-12 / STAT4 and the like, and the data dimension and reliability of preclinical immunological evaluation are remarkably enhanced.

The invention discloses a yellow thorn moth blood lymphocyte line, an expanded production method of a nuclear polyhedrosis virus and application of the yellow thorn moth blood lymphocyte line and the nuclear polyhedrosis virus. The name of the dalbergia mandshurica blood lymphocyte line is dalbergia mandshurica blood lymphocyte CFW-CHY, the dalbergia mandshurica blood lymphocyte line is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is CCTCC NO: C2025104, and the preservation date is March 27, 2025; the yellow thorn moth blood lymphocyte line is used for expanded production of the nuclear polyhedrosis virus, so that industrial production of the nuclear polyhedrosis virus is realized, and the yellow thorn moth blood lymphocyte line can be used in the fields of preparation of insecticides, insecticidal pesticides and the like.

The application provides an HE chromatin control product, a preparation method and application thereof, including a control slide, the control slide includes a first smear area and a second smear area, the first smear area and the second smear area are respectively arranged in different areas of the control slide, the first smear area is uniformly coated with mammalian peripheral blood mature red blood cells, and the second smear area is uniformly coated with mammalian peripheral blood lymphocytes. The HE chromatin control product provided by the application includes the combination of the mature red blood cell first smear area and the lymphocyte second smear area, can respectively and specifically reflect the staining effect of cytoplasm and nucleus in HE staining, realizes independent quality control of nucleus and plasma, the blood cell source is uniform, the difference of human body structure can be eliminated, batch uniformity and stability can be ensured, quantitative detection can be realized through a microspectrophotometer, objective and quantitative staining quality can be realized, the subjective and standardized difficulty problem of traditional quality control can be solved, and the accuracy and consistency of pathological quality control are improved.

The invention discloses an in-vitro culture method of primary blood lymphocytes of crassostrea gigas and application of the primary blood lymphocytes. The in-vitro culture method of the primary blood lymphocytes of the crassostrea gigas comprises the following steps: inoculating suspension of the primary blood lymphocytes of the crassostrea gigas into a culture plate containing a culture medium, and culturing at constant temperature. The culture medium comprises a basic culture medium, glucose, a mixed salt solution, a 1wt% penicillin-streptomycin mixed solution and 50% crassostrea gigas serum, and the pH value of the culture medium is 7.0, and the culture medium is 1000 mOsm. The culture medium for the primary blood lymphocytes of the crassostrea gigas contains 50% of crassostrea gigas serum, simulates the growth environment of the blood lymphocytes, provides nutrient substances, cell factors, antibacterial substances and the like required by the growth of the blood lymphocytes, and is more beneficial to the growth of the primary blood lymphocytes of the crassostrea gigas.

The invention discloses a lung cancer patient prognosis evaluation method and system combining pathological images and blood indexes. The method comprises the steps that reference pathological features and reference blood clinical indexes of a lung cancer patient in different clinical stages are obtained, and a lung cancer stage evaluation model is constructed; acquiring a real-time pathological image and a blood detection report of the target patient, and determining a current lung cancer development stage of the target patient through the lung cancer stage evaluation model; determining the risk attribute of the target patient based on the current lung cancer development stage and determining the blood lymphocyte proportion of the target patient; determining a key blood index of the target patient according to the blood lymphocyte proportion, and outputting a prognosis risk score and a lifetime prediction result of the target patient through a preset survival analysis model according to the key blood index and the multi-scale morphological characteristics. The objectivity, the high precision and the stability of the prediction result are ensured, the interference of human subjective factors is eliminated, and the reliability is improved.

The invention discloses a specific antibody for resisting Crassostrea gigas granulocyte membrane surface differentiation antigen protein CD22, the specific antibody is obtained by immunizing Balb / c mice by using recombinant protein of the Crassostrea gigas granulocyte membrane surface differentiation antigen protein CD22, and the recombinant protein completely contains all extracellular Ig, IGc2 and Ig-like functional domains of the Crassostrea gigas granulocyte membrane surface differentiation antigen protein CD22. The specific antibody can specifically recognize a CD22 recombinant protein with the molecular weight of 61.3 kDa and a blood lymphocyte natural protein with the molecular weight of 102.8 kDa, and it is detected that the CD22 protein is mainly expressed on cell membranes of part of agranulocytes. The specific antibody provided by the invention can be used for identifying and sorting the Crassostrea gigas CD22 + agranulocyte, and provides an important tool for deeply researching the phenotype and function of the CD22 + agranulocyte and the effect of the CD22 + agranulocyte in immune response.

The invention belongs to the technical field of biology, and particularly relates to a sheep-derived anti-bluetongue virus recombinant neutralizing antibody and a preparation method thereof. An anti-bluetongue virus specific B cell population is screened from sheep peripheral blood lymphocytes, a sheep anti-bluetongue virus antibody database is obtained through a single cell V (D) J sequencing technology, and the sheep recombinant antibody is successfully expressed after screening. Experiments such as Western-blot, cellular immunofluorescence, indirect ELISA and microneutralization prove that the antibody can be combined with bluetongue virus coat protein VP2 and has a good neutralization effect, a key tool is provided for rapid and typing detection of bluetongue, a foundation is laid for research and development of a novel efficient bluetongue vaccine, and the antibody has a good application prospect. The method is of great significance to further development of bluetongue prevention and control technology research.

The application discloses application of peripheral blood lymphocyte subgroups in preparation of a detection kit for evaluating prognosis of anti-PD-1 treatment of a patient with advanced non-small cell lung cancer. The kit is used for determining the proportion of lymphocyte subgroups in peripheral blood of the patient with advanced non-small cell lung cancer after receiving the anti-PD-1 treatment, so as to evaluate the prognosis of the patient with advanced non-small cell lung cancer. According to the embodiment of the application, the anti-PD-1 treatment effect of the patient with advanced NSCLC can be known in time by detecting the change of peripheral blood lymphocyte subgroups of the patient with advanced NSCLC.

The invention discloses a crassostrea gigas offspring seed cultivation method capable of improving immunity of D-type larvae, which comprises the following steps: in the crassostrea gigas offspring seed cultivation process, when crassostrea gigas fertilized eggs develop to the initial stage of the D-type larvae, culturing the D-type larvae for 24 hours by using a culture water body containing ammonium chloride with the final concentration of 0.053 g / L, and then transferring the D-type larvae into a culture water body without ammonium chloride to be continuously cultured. According to the invention, the autophagy level of the D-type larvae can be obviously improved, the proliferation of blood lymphocytes of the D-type larvae is promoted, and the immune response capability of the D-type larvae to viruses is enhanced. In addition, ammonium chloride can also induce expression of inflammation-related genes of the D-type larvae, so that the immunity of the D-type larvae of the crassostrea gigas is comprehensively improved, the death rate of the larvae is effectively reduced, and the quality of crassostrea gigas fries is improved. Meanwhile, the method has the advantages of simplicity in operation, low cost and the like.

The invention discloses a low-oxygen-resistant screening device and method for eriocheir sinensis. The device comprises a low-oxygen water tank, a dissolved oxygen control module, a lifting plate module, a vibration stimulation module, a gradient escape channel plate, a crab collection area, a dissolved oxygen utilization control module and a climbing behavior excitation module. The method comprises the following steps: (1) pre-adapting; (2) hypoxia stress; (3) stress triggering; and (4) escape screening and the like, as well as blood lymphocyte counting, measurement of hemocyanin and the like and hypoxia median lethal time verification, efficient and accurate breeding of hypoxia-resistant characters is realized.