16 results about "Lymphocyte subsets" patented technology

The invention provides application of a substance for detecting the ratio of lymphocyte subgroups in preparation of a reagent or a kit for diagnosis or auxiliary diagnosis of Graves disease, and belongs to the technical field of preparation of disease diagnosis reagents. A research shows that compared with a healthy sample, the proportion of B cells in a lymphocyte subpopulation of a to-be-detected sample is remarkably increased, the proportion of NK cells is remarkably reduced, the proportion of activated T cells is remarkably reduced, the proportion of memory T cells is remarkably reduced, the proportion of CD4 + memory T cells is remarkably reduced, the proportion of Th1 cells is remarkably reduced, and the proportion of Tc1 cells is remarkably reduced, so that the to-be-detected sample is a GD patient; the substance for detecting the ratio of lymphocyte subgroups is high in sensitivity and specificity when being used for diagnosis or auxiliary diagnosis of Graves disease.

The present application belongs to the field of biomedical technology, and relates to a human papilloma virus (HPV) infection immune response related detection method and application. The present application provides a new method for detecting HPV infection, which comprises detecting virus load, DC maturation state in peripheral blood, or T lymphocyte subgroups and NK cell surface markers. The present application adopts a combined detection technology of DC cells, T lymphocytes and NK cells, and is used for detecting the immune response state of a host after HPV infection. The present application also provides application of the detection method. The detection method of the present application is simple and clear in result, and can be applied to clinical auxiliary detection and examination of HPV and even cervical cancer, and screening of HPV and even cervical cancer.

The invention discloses a child antinuclear antibody positive immune thrombocytopenia patient database establishment method and device. The child antinuclear antibody positive immune thrombocytopenia patient database establishment method comprises the following steps: acquiring a child patient basic screening data set and child patient subdivision clinical data provided by multiple centers; acquiring a structured full-dimensional data set of the child patient; carrying out data standardization and unified coding processing on the data so as to form a child patient standardization data set; performing permission setting distribution on each piece of data in the child patient standardized data set to form an encrypted security data set; and constructing a child antinuclear antibody positive immune thrombocytopenia patient database according to the encrypted security data set. According to the database established by the method disclosed by the invention, immune characteristics such as antinuclear antibody spectrum, anti-ENA spectrum, complement, lymphocyte subpopulation and the like as well as platelet level, clinical manifestation and treatment reaction are comprehensively recorded, so that a doctor can pay attention to immunology abnormality of a patient earlier during recording.

The present application relates to the technical field of biological cell collection and preservation, in particular to a peripheral blood immune cell efficient collection and low-temperature storage method, comprising: collecting peripheral blood samples and obtaining donor health status information, generating a sample cell composition atlas containing lymphocyte subpopulation absolute count percentage, monocyte purity and granulocyte contamination ratio through full-automatic blood cell analysis, determining the target immune cell optimal collection amount and adaptive collection process through a preset data analysis model, enriching target cells through gradient density centrifugation and specific magnetic bead sorting, comparing and judging supplementary collection by combining cell quality inspection data and expected collection amount, and then calculating individualized cryopreservation liquid formula, cooling program and storage strategy through a low-temperature storage optimization algorithm. The method can realize precise individualized adaptation of the collection process and storage parameters, and improve the cell enrichment purity and the cell state stability after low-temperature storage.

The invention belongs to the technical field of biological medicine, and particularly relates to safflower seed meal protein and peptide as well as a preparation method and application thereof. The safflower seed meal protein and peptide provided by the invention are prepared through the steps of degreasing, alkali extraction, ultrasonic-assisted extraction, acid precipitation, ammonium sulfate precipitation, enzymolysis and the like, and have good antioxidant activity. Animal experiments show that the protein and the peptide can effectively relieve DSS-induced mouse colitis, remarkably improve hematochezia, diarrhea, colon shortening and other symptoms, regulate immune organ indexes and T lymphocyte subgroups and enhance the oxidation resistance of intestinal tracts, and do not show toxicity under high dosage. The traditional Chinese medicine composition can be effectively used for preparing a medicine for treating colitis, and a natural and safe new candidate medicine is provided for colitis treatment.

The application discloses an immune dynamic trajectory modeling method and system, which solves the problems that the prior art cannot accurately locate the inflection point of the change of the immune parameter with age, cannot comprehensively evaluate the immune state of multiple parameters, and lacks visual dynamic trajectory output. The method comprises the following steps: collecting multiple lymphocyte subpopulation parameters in peripheral blood; detecting the trend of each immune parameter with age, calculating the change slope before and after each inflection point; dimension reduction and weighting are performed on multiple immune parameters to construct a comprehensive immune index (CII); and a dynamic trajectory graph containing the inflection point age of each immune parameter, the change slope and the comprehensive index trend graph is output. The application integrates flow cytometry detection data, piecewise regression model and principal component analysis model, can intuitively display the nonlinear and multi-stage characteristics of the evolution of the immune system with age, identify the key aging inflection point, and provide a quantitative tool for population immune state screening, aging mechanism research and drug or nutrition intervention effect evaluation.

The present application relates to the technical field of medical quality control products, in particular to a quality control substance for lymphocyte subset analysis and a preparation method thereof, comprising human monocyte simulation particles, human granulocyte simulation particles, human lymphocyte simulation particles, human red blood cell simulation particles, human platelet simulation particles and a preservative, wherein the human monocyte simulation particles, the human granulocyte simulation particles, the human lymphocyte simulation particles, the human red blood cell simulation particles and the human platelet simulation particles are added into the preservative, and mixed according to the concentration of human particles to obtain the quality control substance for lymphocyte subset analysis. Compared with the prior art, the quality control substance for lymphocyte subset analysis and the preparation method thereof have the advantages of simple operation, small batch difference and good stability, and can be applied to the field of quality control of flow cytometry methodological lymphocyte subset analysis.

The invention belongs to the technical field of biomedicine, and particularly relates to application of periplaneta americana polypeptide and periplaneta americana peptide B serving as an immunologic adjuvant. The amino acid sequence of the periplaneta americana polypeptide and periplaneta americana peptide B is shown as SEQ ID NO.1. According to the invention, influenza virus H1N1RBD protein is combined with periplaneta americana peptide B, mice can be induced to generate an anti-RBD protein specific IgG antibody with higher titer, and the titer of the antibody can be increased along with the increase of immunization times. Meanwhile, the level of T subsets of lymphocytes of a mouse body can be changed, so that the T subsets can resist pathogen invasion to a certain extent. The periplaneta americana polypeptide periplaneta americana peptide B provided by the invention can be conveniently obtained through mass synthesis, can be used as a novel micromolecular immunologic adjuvant, and is beneficial to enhancing the specific immune response of an organism.

The invention discloses an immune cell counting detection method, and belongs to the technical field of immune cell detection. According to the invention, the problem of one-sidedness of the existing detection method is solved, the immune assessment is divided into three levels, and the first level structure detects the proportion and absolute count of the basal lymphocyte subpopulation; the secondary structure deeply analyzes functional subtypes of T cells, including Th1 / Th2 / Th17, initial / memory / activated T cells and regulatory T cells; the tertiary structure is used for evaluating cytokine expression and immune checkpoint molecules; performing multi-parameter detection on the same biological sample through optimized multicolor flow cytometry, and generating a comprehensive immune assessment report; therefore, the whole state and potential problems of the immune system can be intuitively reflected, popularization and application in a clinical examination laboratory are facilitated, comprehensive and deep immune information from the number of cells to the functional state is clinically provided, and the method has important value for diagnosis, treatment monitoring and prognosis evaluation of clinical immune related diseases.

The invention relates to the field of medical treatment, and discloses an antibody composition for lymphocyte subpopulation analysis, a detection kit, a detection method and application, and the antibody composition comprises an anti-CD38 antibody, an anti-CD45RA antibody, an anti-CD57 antibody, an anti-CD45 antibody, an anti-CD27 antibody, an anti-CD16 antibody, an anti-CD56 antibody, an anti-CD3 antibody, an anti-CD4 antibody, an anti-CD8 antibody, an anti-CD19 antibody and an antiTCR-alpha-beta antibody. 12 antibodies are combined, the cell proliferation condition and activation condition of a person to be detected can be detected, the detection items are comprehensive and highly targeted, the prognosis condition of a tumor patient can be well reflected, reference is provided for prevention and relapse, meanwhile, the combination of the antibodies is marked, and the detection accuracy is improved. Under the condition of an existing 10-color flow cytometry instrument, 12 CD can be detected at a time, the requirement for the sample size is obviously reduced, and the detection speed is increased.

The invention is applicable to the technical field of biomedicine and immunological detection, and provides a reagent composition, a kit and a method for risk prediction of senile deafness, the reagent composition contains three types of fluorescence labeled antibody combinations: T cell surface marker antibodies CD3, CD4 and CD8; a T cell differentiation and activation marker antibody CCR7, CD45RA, HLA-DR and CD38; and natural killer cell functional marker antibodies CD56 and NKP30. The expression level of related lymphocyte subpopulations and functional markers thereof in peripheral blood is detected through flow cytometry, the'immune-auditory axis' association of immune aging and auditory degeneration is revealed, and a multi-parameter prediction model integrating age and immune indexes is constructed. The model can realize early recognition, risk stratification and accurate evaluation of senile deafness of middle-aged and elderly people. The method has the advantages of high prediction accuracy, early warning, non-invasiveness, simplicity and convenience in operation and the like, and has a wide application prospect.

The application provides a lung cancer immune suppression state evaluation method based on lymphocyte subgroups, and belongs to the technical field of immune suppression state evaluation.The application performs PHATE algorithm manifold dimension reduction and Harmony batch effect correction on high-dimensional data obtained by multicolor flow cytometry, retains the global topological structure of rare lymphocyte subgroups, eliminates cross-batch systematic deviation, extracts batch-invariant cell representation through an adversarial domain adaptive network, identifies lymphocyte subgroups by using graph clustering analysis and constructs a patient-level immune phenotype feature vector, performs Kalman filtering on multi-time-point longitudinal data to build a time series state space model, and finally jointly optimizes the clustering threshold and the routing gating threshold through an artificial intelligence model and a game optimization framework, so that the technical problem of missing detection of rare lymphocyte subgroups caused by systematic deviation during cross-batch integration of multi-batch high-dimensional flow cytometry data is solved.

Disclosed herein are methods for treating a cancer comprising: a. administering a Btk inhibitor to a subject sufficient to result in an increase or appearance in the blood of a subpopulation of lymphocytes defined by immunophenotyping; b. determining the expression profile of one or more biomarkers from one or more subpopulation of lymphocytes; and c. administering a second agent based on the determined expression profile.

The application provides application of a substance for detecting lymphocyte subpopulation ratio in preparation of a Graves' disease diagnosis or auxiliary diagnosis reagent or kit, and belongs to the technical field of disease diagnosis reagent preparation. Research finds that, compared with a healthy sample, the proportion of B cells in a lymphocyte subpopulation of a sample to be detected is significantly increased, the proportion of NK cells is significantly reduced, the proportion of activated T cells is significantly reduced, the proportion of memory T cells is significantly reduced, the proportion of CD4+ memory T cells is significantly reduced, the proportion of Th1 cells is significantly reduced, and the proportion of Tc1 cells is significantly reduced, so that the sample to be detected is a GD patient, and the application has high sensitivity and strong specificity in diagnosis or auxiliary diagnosis of Graves' disease by using the substance for detecting lymphocyte subpopulation ratio.

The application relates to a lymphocyte subpopulation and cytokine combined detection method, kit and application based on flow cytometry. The method constructs a parallel detection system of cell phenotypes and cytokines on the same flow platform, solves the signal interference problem by using spectrum compatible markers, and realizes synchronous analysis of immune cell subpopulations, immune checkpoints and multiple cytokines in trace blood samples. The scheme avoids system errors and sample waste caused by multi-platform detection, can provide a comprehensive atlas reflecting the synergistic state of cell immune structure and humoral immune function, and improves the accuracy and efficiency of clinical immune evaluation.

The application discloses application of peripheral blood lymphocyte subgroups in preparation of a detection kit for evaluating prognosis of anti-PD-1 treatment of a patient with advanced non-small cell lung cancer. The kit is used for determining the proportion of lymphocyte subgroups in peripheral blood of the patient with advanced non-small cell lung cancer after receiving the anti-PD-1 treatment, so as to evaluate the prognosis of the patient with advanced non-small cell lung cancer. According to the embodiment of the application, the anti-PD-1 treatment effect of the patient with advanced NSCLC can be known in time by detecting the change of peripheral blood lymphocyte subgroups of the patient with advanced NSCLC.