The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a compound traditional Chinese medicine composition for regulating tumor immunity and resisting tumors as well as a preparation method and application thereof. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 5-15 parts of lucid ganoderma, 15-25 parts of astragalus membranaceus, 5-15 parts of ginseng, 5-15 parts of roasted rhizoma atractylodis macrocephalae, 5-15 parts of poria cocos, 5-15 parts of angelica sinensis, 5-15 parts of radix sophorae flavescentis and 5-15 parts of honey-fried licorice root. The compound traditional Chinese medicine composition provided by the invention has the effects of combining purgation and tonification, replenishing qi and enriching blood, detoxifying and removing stasis, regulating tumor immunity, resisting tumors and the like, can regulate and control tumor immune checkpoint proteins, has a killing effect on various tumor cells, can regulate the proportion of a plurality of T lymphocyte subpopulations, causes remarkable increase of immunocompetent cells, and has a remarkable curative effect on tumor cells. The immune function is enhanced, and the effect of treating tumors is achieved.