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Human peripheral blood lymphocyte culture medium

A lymphocyte and culture medium technology, applied in the field of human peripheral blood lymphocyte culture medium and its preparation, can solve problems such as unstable pH, easy to be infected by viruses or microorganisms, lack of nutrients, etc., and achieve good reagent stability and effective Effects of inhibiting microorganisms and viruses and promoting cell proliferation

Inactive Publication Date: 2017-05-31
JIANGXI YIXINTANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] Aiming at the problems that existing cell culture media are easily infected by viruses or microorganisms, lack of nutrients and unstable pH, the present invention provides a culture medium for human peripheral blood lymphocytes and a preparation method thereof, which can effectively inhibit the effects of microorganisms and viruses , can promote cell proliferation, shorten the cell culture cycle, has the advantages of high lymphocyte transformation rate and lymphocyte division index, and good reagent stability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 A kind of human peripheral blood lymphocyte culture medium

[0027] A culture medium for human peripheral blood lymphocytes, consisting of the following components and their concentrations: RPMI1640 medium 55g / L, Ham's12 medium 27g / L, bovine serum 1.68g / L, hyaluronic acid 3.75g / L, phenolic acid Protein phosphopeptide 4.12g / L, lectin 5.32g / L, wolfberry polysaccharide 89.3μg / mL, baicalin 4.2g / L and nisin 0.58g / L.

[0028] The preparation method of the above-mentioned medium is: (1) accurately weigh the dry powder of RPMI1640 and Ham's12 medium and place it in a sterile container, add ultrapure water to dilute and mix well to obtain solution I; (2) pasteurize (3) Add PHA, hyaluronic acid, casein phosphopeptide, Lycium barbarum polysaccharide, baicalin, and nisin into solution II, and mix thoroughly Evenly put negative pressure filter sterilization, that is.

Embodiment 2

[0029] Embodiment 2 A kind of human peripheral blood lymphocyte culture medium

[0030] A culture medium for human peripheral blood lymphocytes, consisting of the following components and their concentrations: RPMI1640 medium 50g / L, Ham's12 medium 20g / L, bovine serum 1g / L, hyaluronic acid 3-g / L, casein Protein phosphopeptide 3g / L, wolfberry polysaccharide 85mg / L, lectin 4.5g / L, baicalin 1g / L and nisin 0.1g / L.

[0031] The preparation method of the above medium is similar to that of Example 1.

Embodiment 3

[0032] Embodiment 3 A kind of human peripheral blood lymphocyte culture medium

[0033] A culture medium for human peripheral blood lymphocytes, consisting of the following components and their concentrations: RPMI1640 medium 56g / L, Ham's12 medium 30g / L, bovine serum 2g / L, hyaluronic acid 4.5g / L, casein Phosphopeptide 4.5g / L, wolfberry polysaccharide 95mg / L, lectin 5.5g / L, baicalin 5g / L and nisin 0.7g / L.

[0034] The preparation method of the above medium is similar to that of Example 1.

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PUM

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Abstract

The invention belongs to the technical field of cell culture media, and concretely relates to a human peripheral blood lymphocyte culture medium, which mainly contains the following components and concentration thereof: an RPMI1640 liquid culture medium, Ham's 12, bovine serum, hyaluronic acid 3, casein phosphopeptides, phytagglutinin, lyciumbarbarum polysaccharide, baicalin and nisin. The culture medium provided by the invention can be beneficial for effectively inhibiting actions of microorganisms and viruses, promoting cell proliferation, and shortening cell culture cycle, and has the advantages of high lymphocyte conversion rate and lymphocyte division index, and good reagent stability.

Description

technical field [0001] The invention belongs to the technical field of cell culture medium, and in particular relates to a human peripheral blood lymphocyte culture medium and a preparation method thereof. Background technique [0002] The small lymphocytes in human peripheral blood are almost in the GI phase (GO phase), and generally do not divide anymore. When phytohemagglutinin PHA is added to the culture medium, these small lymphocytes are stimulated to transform into lymphocytes. Mother cells then enter mitosis, so after short-term culture, colchicine treatment, hypotonicity and fixation, some mitotic cells can be obtained. This method has been widely used in clinical medicine, virology, pharmacology, genetic toxicity and so on. [0003] At present, the reasons that affect the growth of human peripheral blood lymphocytes are: [0004] (1) Virus or microbial infection: When cells are cultured in vitro, compared with in vivo cells lose their defense against microorganis...

Claims

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Application Information

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IPC IPC(8): C12N5/078
CPCC12N5/0634C12N2500/72C12N2500/76C12N2501/90C12N2501/905C12N2501/998
Inventor 胡波钟勇财谢玉国
Owner JIANGXI YIXINTANG MEDICAL TECH
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