67 results about "Lymphocyte transformation" patented technology

A Chinese medicine for regulating immunity and suppressing tumor is prepared from pilose asiabell root and astragalus root (1:1). Its preparing process is also disclosed.

The invention belongs to the medical technical field and discloses the application of high-purity in the preparation of antibacterial medicine, antiviral medicine and other medicines. The invention proves the antipyretic, anti-inflammation, immunity enhancement, antibacterial and anti-virus effects of high-purity forsythiaside through the following experiments: (1) the experiment that high-purity forsythiaside has antipyretic effect on fever rats; (2) the experiment that high-purity forsythiaside inhibits xylene-induced mice ear edema, xylene-reduced capillary permeability of mice; (3) the experiment that high-purity forsythiaside increases carbon clearance ability of mice and increases the lymphocyte transformation rate; (4) the experiment that high-purity forsythiaside has vitro anti-influenza A type virus effect and in-vivo anti-respiratory syncytial virus effect; (5) the experiment that high-purity forsythiaside has vitro inhibition effects on beta-hemolytic streptococcus, staphylococcus aureus, streptococcus viridans and staphylococcus epidermidis.

The invention aims to provide lactobacillus rhamnosus having bacteriostatic and immunity enhancement function, with the preservation number: CCTCC NO: M 2013355. The lactobacillus rhamnosus provided by the invention can be applied in the biological products which are medicines, food or health products. The lactobacillus rhamnosus has very powerful bacteriostatic ability, can obviously improve the achroacyte conversion and HCso of a mouse, phagocytosis rate and index of macrophage and NK cell activity, can enhance normal mouse immunity, and therefore can be widely applied in the medicines, food or the health products.

The invention discloses a health product for improving bone density and immunity, which active ingredients have the following weight ratios: 60-140 radix puerariae extract, 120-280 D-dextrosamine and 120-280 calcium. The advantages of the health product in the invention are in that: rapid releasing, easy for absorbing, improving arthrosis pain caused by bone loss, preventing and treating various diseases caused by cartilage tissue cataplasia; distinctly increasing ingestion action of liver and spleen macrophage to ingest carbon granules, distinctly accelerating lymphocyte transformation and raising phagocytosis rate of the macrophage to obviously improve nonspecific immunity function of human body. Accordingly, the invention has good and fast effect for increasing bone density and immunity.

The invention relates to an infusion protein of an IDB (Infectious Bursal Disease) virus VP2 protein and an LTB (Leukotrienes B) of enterotoxin of escherichia coli, wherein the amino acid sequence of the infusion protein is SEQ ID NO: 1. According to the invention, the supervirulent VP2 protein of the IBDV (Infectious Bursal Disease Virus) is expressed with the LTB protein of enterotoxin of escherichia coli with a mucosal immunologic adjuvant in a fusion manner, and an expression product is emulsified to prepare a vaccine to make SPF chickens which are 21 days old immune. An immune reference and a non-immune reference for a subunit vaccine of VP2 are set. After 7 days of immunity, the lymphocyte transformation rate is determined, and after 21 days of immunity, blood is collected to determine the valence of antibody of immunodiffusion of serum agar and toxic substances are counteracted together with the non-immune reference. The result shows that vaccine immunity groups can resist virulent attach. The lymphocyte transformation rate and the serum valence of antibody are remarkably higher than the immune reference group of VP2, so that the infusion protein has an excellent immunogenicity.

The invention provides a method for preparing natural antibiotics- recombined chicken- beta Buchner's bodies Gal-9. The invention employs molecular biology technology to clone chicken- beta Buchner's bodies Gal-9 gene from chicken, and expresses chicken- beta Buchner's bodies Gal-9 protein outside chicken body by using proper expression host. It is found by further research that the chicken- beta Buchner's bodies Gal-9 possesses wide anti bacteria activity and immunologic enhancement. The recombined chicken- beta Buchner's bodies Gal-9 is characterized in that: (1) ir can inhibit and kill bacillus coli, salmonella, Micrococcus pyogenes and so on, (2) prevents avian infectious brunchitis virus, (3) increases antibody for avian infectious brunchitis virus concentration by 10- 20 % in dimefox after oral administration for one week by chicken, improves apleen lymphocyte blastogenesis by 40- 50%.

The invention provides a method for preparing natural antibacterial agent- recombination chicken- beta defensins Gal-9-Gal-8 bi-molecule protein. The invention employs molecular biology technology and colones chicken- beta defensins Gal- 8 and Gal-9 gene inside chicken and expresses defensins Gal-9-Gal-8 bi-molecule protein outside chicken body by employing proper expression host. The recombined chicken- beta defensins Gal-9-Gal-8 bi-molecule protein is characterized in that: (1) it can inhibit and kill bacillus coli, salmonella and salmonella and so on, (2) it can resist avian infectious brunchitis and newcastle disease virus, (3) it can increase the antibody for avian infectious brunchitis in dimefox by 10-20%, antibody for newcastle disease virus by 10-20% and increase chicken splenocyte lymphocyte conversion rate by 60-70%.

The invention belongs to the technical field of cell mediums, and in particular relates to a human peripheral blood lymphocyte medium and a preparation method thereof. The medium provided by the invention mainly consists of the following components: an RPMI1640 medium, fetal bovine serum, collagen, sodium hyaluronate, glucagon, thyrotropin releasing hormone, total glucosides of paeony, linoleic acid, butanediamine, gluconic acid iron salt hydrate, glutathione and polylysine. The medium provided by the invention can be used for effectively inhibiting microorganisms and viruses, and the medium is good in reagent stability and high in transformation rate of cultured lymphocytes and lymphocyte division index.

The invention belongs to the technical field of cell culture media, and concretely relates to a human peripheral blood lymphocyte culture medium, which mainly contains the following components and concentration thereof: an RPMI1640 liquid culture medium, Ham's 12, bovine serum, hyaluronic acid 3, casein phosphopeptides, phytagglutinin, lyciumbarbarum polysaccharide, baicalin and nisin. The culture medium provided by the invention can be beneficial for effectively inhibiting actions of microorganisms and viruses, promoting cell proliferation, and shortening cell culture cycle, and has the advantages of high lymphocyte conversion rate and lymphocyte division index, and good reagent stability.

An application of baikal skullcap polysaccharides in preparation of a veterinary drug is disclosed. The baikal skullcap polysaccharides are used for preparing an additive in a veterinary drug enhancing immune functions of animals, and are prepared through extraction of baikal skullcap plants. The concentration of the baikal skullcap polysaccharides in the veterinary drug is 31.25 [mu]g/mL to 4 mg/mL. Pig lymphocyte transformation tests show that the baikal skullcap polysaccharides can promote lymphocyte transformation, can increase contents of the total protein (TP), serum albumin (ALB), serumglobulin (GLOB), lymphocytes and monocytes in serum of rats with low immunity so as to restore body conditions of the rats, thus proving that the baikal skullcap polysaccharides have effect of significantly enhancing animal body immune functions and can be used in a veterinary drug enhancing animal body immune functions.

The invention discloses compound astragalus polysaccharide injection used for pigs and a preparation method thereof. Every 100L of compound astragalus polysaccharide injection used for the pigs contains the following raw materials, such as 0.5-5kg of astragalus polysaccharide, 1-10kg of lincomycin, 5-30kg of analginum, 1-10kg of inosine, 3-6L of 2-hydroxyl ethylamine and the like, wherein the astragalus polysaccharide can accelerate the lymphocyte to be converted and increased in value and accelerate the phagocytic function of macrophage; the lincomycin has strong antibiosis efficacy; the astragalus polysaccharide and the lincomycin are organically combined to perform an antiviral function, widen an antibacterial spectrum and enhance a targeted action on bacteria; the analginum has various functions of resisting bacterium, diminishing inflammation, carrying out antipyretic action, relieving pain and the like; and the inosine is favorable for restoring the function of a damaged hepaticcell. Above medicines have a reasonable formula, can simultaneously carry out symptomatic treatment and etiological treatment by aiming at diseases, has quick curative effect on mixed infection of pig virus and bacteria and treats both principal and secondary aspect of the disease, the medicine resistance of the pathogenic microorganism is lowered, the clinic symptom of a diseased pig is alleviated, and therefore the pig is quickly restored.

The invention discloses an application of a rodgersia sambucifolia extract in preparation of a veterinary drug. The rodgersia sambucifolia extract is used for preparing a drug additive of the veterinary drug for enhancing the immune function of animal bodies, the rodgersia sambucifolia extract is a crude flavonoid extract extracted from a rodgersia sambucifolia plant, the crude flavonoid extract of rodgersia sambucifolia is applied to the drug additive of the veterinary drug, and the drug concentration in the veterinary drug is 1.25-5 mg/mL. The obtained crude flavonoid extract of rodgersia sambucifolia has good biological activity and can promote transformation of lymphocytes in swine lymphocyte transformation experiments, increase the content of WBCs (white blood cells), Gran (neutrophilicgranulocyte) and Lym (lymphocyte) in serum of rats with low immunogenicity and recover the physical condition of rats with immunosuppression. It is indicated that the crude flavonoid extract of rodgersia sambucifolia has the effect of significantly enhancing the immune function of animal bodies and can be used as a raw material of the veterinary drug for enhancing the immune function of animal bodies.

The invention relates to a preparation method of a wood frog spawn polysaccharide extract, and an application of the wood frog spawn polysaccharide extract in immunity enhancement. The method is characterized in that the method comprises the following steps: extracting wood frog spawn polysaccharides from wood frog spawn as a raw material through an enzymatic hydrolysis technology, filtering or centrifuging, carrying out alcohol precipitation on the above obtained filtered or centrifuged solution with anhydrous ethanol 3 times the volume of the filtered or centrifuged solution to obtain a precipitate which is the polysaccharide extract, freeze-drying or oven-drying to prepare an extract or dry powder; and the obtained ethanol liquid is recovered below 40DEG C. The wood frog spawn polysaccharides have mouse lymphocyte conversion promotion effects on in vitro mouse spleens to different degrees, a good dose-effect relationship exists, and the polysaccharide extract with the concentration of 0.001[mu]g/mL still has a strong lymphocyte conversion promotion effect. In mouse in vivo experiments, a wood frog spawn polysaccharide sample has an obvious lymphocyte conversion promotion effect. In the field of the influences on cytokines in mouse serum, the influences of the wood frog spawn polysaccharides on the outputs of TNF-alpha and IL-1beta in the serum substantially change. The wood frog spawn polysaccharide extract can be used to prepare immune medicines, and can also be used as a food additive. The extraction method and the extract solvent are not limited to that mentioned in the experiments and the embodiments.

The invention relates to an anti-tumor traditional Chinese medicinal composition. The anti-tumor traditional Chinese medicinal composition is prepared from the following raw medicinal materials by weight: 1-7 g of radix aconiti kusnezoffii, 1-10 g of radix aconiti lateralis, 1-10 g of rhizoma pinelliae, 1-10 g of rhizoma arisaematis, 1-10 g of thallus laminariae et eckloniae, 1-10 g of borneol, 1-10 g of cortex cinnamomi, 5-25 g of radix glycyrrhizae, 0.5-5 g of calomelas, 2-15 g of centipedes, 2-15 g of spiders, 1-10 g of mylabris, 2-20 g of radix ginseng rubra, 2-15 g of colla cornus cervi,3-15 g of radix notoginseng, 3-15 g of scorpio, and 2-15 g of squama manitis. The anti-tumor traditional Chinese medicinal composition provided by the invention is used for dredging the channels, stimulating the vitality of macrophages, improving the transformation rate of lymphocytes, inhibiting the growth of cancer and tumor cells, damaging the fibrous protein agglutination around the tumor andin the foci, preventing implantation of cancer cells, and preventing the growth and metastasis of tumor.

The invention discloses a composition for enhancing the immunity and protecting gastric mucosa and a use method, and belongs to the field of composition chewable tablets. The composition comprises, byweight, 140-160 parts of hericium erinaceus extracts, 70-90 parts of fructus lycii extracts, 70-90 parts of poria cocos extracts, 70-90 parts of Chinese date extracts, 240-260 parts of starch, 70-90parts of beta- cyclodextrine, 8-9 parts of magnesium stearate, 4-6 parts of silicon dioxide, 5-7 parts of maize germ oil, 240-280 parts of fruit-vegetable powder and 1-2 parts of pseudo-ginseng powder. The mixture A is put in a tabletting machine for tabletting forming, each tablet of composition has the weight of 1 g, and a user takes the composition twice every day and takes two tablets each time by chewing. The composition enhances the phagocytic function of macrophages, promotes the lymphocyte transformation, excites the immune, resists ageing, replenishes blood and protects the liver.

The invention discloses health-care jelly powder for children. The health-care jelly powder for children is characterized by being mainly prepared from the following components: nut powder, carrot powder, seaweed powder or seaweed micro-slices, carrageenan, konjaku flour, sodium citrate, citric acid, potassium chloride and white sugar. In the health-care jelly powder for children, fatty acids contained in the nut powder are generally unsaturated fatty acids which are very helpful to the intelligence development of children, and the nut powder is rich in vitamin D which has the most important physiological function of promoting calcium absorption and utilization of children; the carrot powder has the effects of benefiting the liver and improving eyesight, benefiting the diaphragm and relaxing the intestines, promoting metabolism, enhancing immunity and the like, and the vitamin A in the carrot powder is capable of improving the eyesight of children, preventing night blindness, promoting skeleton growth, enabling teeth to be solid and promoting children to grow; and the seaweed powder or seaweed micro-slices is or are rich in choline, calcium and iron which are capable of enhancing memory, promoting skeleton growth, promoting tooth growth and enabling the teeth to be healthy, and polysaccharides contained in the seaweed powder or seaweed micro-slices have functions of obviously enhancing cell immunity and humoral immunity and are capable of promoting lymphocyte transformation and improving body immunity.

The invention relates to the technical field of medical detection, and particularly relates to a method for detecting lymphocyte transformation by utilizing a medicament cytochalasins B. According to the invention, the principle that peripheral blood lymphocytes can be transformed into lymphoblasts which are divisive after being stimulated by phytohemagglutinin in vitro and the principle that the cytochalasins B can retard cell division but can not retard cell nucleus division are utilized, 2 Gyg ray irradiation is carried out on the peripheral blood of a human body for further realizing immunodepression, and PHA (phytohemagglutinin) stimulation is carried out and the cytochalasins B is added for treatment, so that the difference between the dikaryon rate of radiated lymphocytes and the dikayron rate of non-irradiated lymphocytes is detected. The result shows that transformed lymphocytes can form dikaryocytes, the dikaryon rate of the radiated lymphocytes can be obviously reduced, the immunity of radiated T lymphocytes can be obviously inhibited, and the dikaryon rate can better reflect the immune function of the lymphocytes. The method is intuitive, simple in operation, rapid, economical, and accurate in result during detection of indexes.

The invention discloses a compound chitosan oligosaccharide meal capable of conditioning symptom of cancer and a preparation method of the compound chitosan oligosaccharide meal. The meal comprises the following raw materials in percentage by mass of 2-4% of chitosan oligosaccharide powder, 43%-53% of compound fungus polysaccharide, 30%-40% of a snow lotus herb culture, and 3%-25% of an astragalusextract. The preparation method of the meal comprises the following steps of separately preparing the chitosan oligosaccharide powder, the compound fungus polysaccharide, the snow lotus herb cultureand the astragalus extract, performing weighing in mass ratio, and performing mixing. The raw materials of the meal are not treated by a conventional crushing and boiling method, but are completely treated by a modern traditional Chinese medicine fermentation extraction technique, hazardous substances in the raw materials are degraded, and active components in traditional Chinese medicines are efficiently obtained, and various active polysaccharide is applied. The active polysaccharide can stimulate immunocompetence, can strengthen the effect of reticuloendothelium for phagocytose of tumor cells, can promote lymphocyte transformation, can activate T cells and B cells, and can promote antibody formation, so that the meal has the activity of resisting tumors to a certain extent.