5 results about "Lymphocyte transformation" patented technology

This invention belongs to the field of plant extract technology, specifically relating to a plant extract composition for enhancing immunity. By weight, it is composed of the following raw materials: 3-5 parts Astragalus membranaceus extract, 2.5-4 parts Echinacea purpurea extract, and 1.5-3.5 parts Rhodiola rosea extract. This plant extract composition, through the scientific formulation of Astragalus membranaceus, Echinacea purpurea, and Rhodiola rosea, and an enzymatic extraction process, enhances the immunomodulatory effect. The combination of the three extracts increases the secretion of TNF-α and IL-6 by macrophages. Furthermore, the spleen lymphocyte stimulation index (SI) of the three extracts reaches 5.57, and the lymphocyte transformation rate increases from 191% to 457%. These results indicate that this plant extract composition effectively enhances the body's non-specific and cellular immune functions by activating macrophages and promoting T lymphocyte proliferation through multiple pathways, providing more comprehensive immune protection.

The application discloses a spinal muscular atrophy quality control product and a preparation method and application thereof. The application aims at the deficiency that the existing method of using Epstein-Barr virus to immortalize lymphocytes cannot efficiently transform spinal muscular atrophy lymphocytes into immortalized cells, and provides a transformation culture medium and a method for constructing immortalized spinal muscular atrophy lymphocytes by using the culture medium. By using the method, the immortalized spinal muscular atrophy lymphocyte strain can be constructed within 30 days, and the corresponding immortalized cell line can be obtained by subculturing the immortalized spinal muscular atrophy lymphocyte strain. When the immortalized spinal muscular atrophy lymphocytes are constructed by using the method, the required time is short, the success rate is high, the genetic background of the obtained immortalized cells is stable, the interoperability with clinical samples is high, the compatibility is good, the immortalized cells can be stably preserved, and the immortalized cells can be used as positive quality control products in gene detection of spinal muscular atrophy, so that the accuracy of detection can be ensured.

This invention provides a 3D-printed hydrogel loaded with mare's milk-derived exosomes, exhibiting pro-inflammatory and antioxidant properties, along with its preparation method and applications, belonging to the field of wound dressing technology. The mare's milk-derived exosomes (M-Exo) of this invention possess excellent pro-inflammatory effects, promoting the high expression of cellular pro-inflammatory factors IL-12p40 and TNF-α, and effectively killing microorganisms in infected wounds. This invention utilizes silk fibroin peptides to encapsulate M-Exo, resulting in drug-loaded nanofiber crystals that serve as a ROS-responsive nano-drug delivery system for M-Exo. When the microenvironment of an infected wound highly expresses ROS, the silk fibroin peptide nanofiber crystals dissolve, releasing M-Exo, thereby increasing T lymphocyte transformation rate, rapidly killing wound microorganisms, and accelerating wound healing.

The invention belongs to the technical field of animal immunopotentiators, and particularly relates to a veterinary immunopotentiator and a preparation method thereof, the veterinary immunopotentiator comprises the following raw materials by weight: 40-50 parts of a traditional Chinese medicine composition, 2-5 parts of a compound amino acid additive, 10-15 parts of a compound vitamin additive, and 2-4 parts of a compound mineral component; wherein the traditional Chinese medicine composition comprises astragalus membranaceus, quercetin, rheum officinale, poria cocos and lentinus edodes; the traditional Chinese medicine composition is added, so that the output of immune globulin can be remarkably increased, interaction between antigen presenting cells and T lymphocytes can be promoted, the conversion rate of the T lymphocytes can be increased, the immunity of livestock can be improved, meanwhile, the compound vitamin additive is added, free radicals in bodies can be removed, oxidative damage to immune cells can be relieved, and the immunity of livestock can be improved. The oxidation process of drugs and vaccine components can be delayed, and the stability and storage period of the drugs are improved, so that the balance of immune effects and side effects is realized.

The invention provides mare milk source exosome-loaded proinflammatory and antioxidant 3D printing hydrogel as well as a preparation method and application thereof, and belongs to the technical field of wound dressing. The mare milk source exosome (M-Exo) has a good pro-inflammatory effect, can promote high expression of pro-inflammatory factors IL-12p40 and TNF-alpha of cells, and can effectively kill microorganisms of infectious wound surfaces. The M-Exo is wrapped by the fibroin peptide, the obtained nanofiber drug-loaded microcrystal can be used as a nano drug-loaded slow release system of M-Exo ROS response, when an infectious wound microenvironment highly expresses ROS, the fibroin peptide nanofiber microcrystal is dissolved, M-Exo is released, the T lymphocyte conversion rate is increased, wound microorganisms are quickly killed, and the wound surface microenvironment is protected. The healing of infectious wounds is accelerated.