2 results about "Peripheral blood lymphocyte" patented technology

This invention discloses a T-cell training method based on tumor organ-on-a-chip. The method includes: preparing an organ-on-a-chip with a micropillar array and a biomimetic fishbone structure; introducing primary tumor cells from a patient into the chip, causing them to spontaneously form uniform three-dimensional tumor spheroids; dynamically pumping pre-activated peripheral blood lymphocytes from the same patient into the chip and co-culturing them with the tumor spheroids; collecting the co-cultured lymphocytes and repeating the co-culture process multiple times to obtain a lymphocyte population with enhanced anti-tumor activity. This invention utilizes organ-on-a-chip technology to highly simulate the dynamic tumor immune microenvironment in vivo. Through multiple cycles of "training," it effectively activates and expands tumor-specific T cells, avoiding immune exhaustion, and providing a highly efficient and controllable new platform for personalized immunotherapy of solid tumors.

This invention belongs to the field of nanobody technology, and relates to an anti-CD63 protein nanobody, its preparation method, and its application. The amino acid sequence of the nanobody is shown in SEQ ID NO.1. This invention utilizes CD63 protein as an immunogen to immunize alpacas, extracts total RNA from their peripheral blood lymphocytes, amplifies the VHH fragment via reverse transcription and two rounds of nested PCR, digests and ligates the VHH fragment with the pComb3XSS plasmid using enzymes, and transforms it into TG1 competent cells. Using phage display technology, after three rounds of screening, an anti-CD63 protein nanobody was successfully screened. This nanobody exhibits high specificity and good binding affinity, providing a new direction for the identification, enrichment, and purification of extracellular vesicles or exosomes, and for disease (such as tumor) diagnosis and targeted drug delivery.