HE chromatin control, preparation method and application thereof

By setting up dual smear regions of mature erythrocytes and lymphocytes from mammalian peripheral blood on a glass slide and combining them with microspectrophotometry, the problem of strong subjectivity and insufficient objectivity in HE staining quality control has been solved, achieving stable and standardized staining quality control, which is suitable for clinical pathology and AI diagnosis.

CN122631407APending Publication Date: 2026-08-25SHENZHEN LONGGANG DISTRICT PEOPLES HOSPITAL
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Patent Information

Application Number
CN202610800964.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2026-06-04
Publication Date
2026-08-25

AI Technical Summary

Technical Problem

Existing HE staining quality control methods rely on subjective human judgment, resulting in poor objectivity and difficulty in achieving quantitative, stable, and standardized quality control, thus failing to meet the needs of clinical pathology testing.

Method used

A HE staining quality control material is provided, comprising setting a first smear area and a second smear area on the same glass slide, respectively smearing mature erythrocytes and lymphocytes from mammalian peripheral blood, detecting the absorbance value using a microspectrophotometer, and determining the quality by combining the absorbance value range with reference absorbance value.

Benefits of technology

It enables independent quality control of nuclear and plasma staining, eliminates the baseline bias caused by differences in human tissue donors, provides a stable and objective evaluation of staining quality, improves the accuracy and consistency of staining quality control, and meets the needs of clinical pathology quantitative quality control and AI diagnosis.

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Abstract

The application provides an HE chromatin control product, a preparation method and application thereof, including a control slide, the control slide includes a first smear area and a second smear area, the first smear area and the second smear area are respectively arranged in different areas of the control slide, the first smear area is uniformly coated with mammalian peripheral blood mature red blood cells, and the second smear area is uniformly coated with mammalian peripheral blood lymphocytes. The HE chromatin control product provided by the application includes the combination of the mature red blood cell first smear area and the lymphocyte second smear area, can respectively and specifically reflect the staining effect of cytoplasm and nucleus in HE staining, realizes independent quality control of nucleus and plasma, the blood cell source is uniform, the difference of human body structure can be eliminated, batch uniformity and stability can be ensured, quantitative detection can be realized through a microspectrophotometer, objective and quantitative staining quality can be realized, the subjective and standardized difficulty problem of traditional quality control can be solved, and the accuracy and consistency of pathological quality control are improved.
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