56 results about "Iridovirus" patented technology

A spore of a bacillus subtilis B.subtilis WB600 strain is used as an antigen delivery carrier, spore coat protein C (CotC) is used as anchoring protein, a key protein ATPase gene and a CotC gene of micropterus salmoides iridovirus are fused through a gene fusion technology, a recombinant spore surface display system is constructed, the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. After being orally applied to fishes, the vaccine can tolerate the gastrointestinal tract environment and is accurately delivered to intestinal related lymphatic tissues to stimulate fish immunity, and finally prevention and control of the iridovirus of the micropterus salmoides are achieved.

The invention discloses a multiplex fluorescent quantitative PCR (polymerase chain reaction) detection kit for prawn culture pathogenic bacteria, which is characterized in that the kit contains primers and probes for detecting the prawn culture pathogenic bacteria, wherein the primers and the probes are respectively used for detecting TDH-related hemolsin genes of vibrio parahaemolyticus, ORF107 genes of white spot syndrome viruses, small subunit ribosomal genes of shrimp enterocytozoon hepatopenaei, 37 kDa coat protein genes of infectious subcutaneous and hematopoietic necrosis viruses, DNA (deoxyribonucleic acid) primer genes of full-eye iridovirus 1 and PirA [pi] genes of pathogens of bacterial prawns acute hepatopancreas necrosis; the method has the advantage that the detection of eight prawn culture pathogens can be realized at the same time. The method comprises the following steps of: obtaining a tcdB gene of a high-pathogenicity vibrio and a hemolsin gene of a vibrio harveyi;

The invention discloses an application of Micropterus salmoides Piscidin polypeptide in preparation of an anti-fish virus drug. The amino acid sequence of the polypeptide is as shown in SEQ ID NO. 2 or SEQ ID NO. 3. The research finds that the micropterus salmoides Piscidin is an antibacterial peptide with an antiviral function, the polypeptide synthesized by the artificially synthesized micropterus salmoides Piscidin has the characteristic of inhibiting fish DNA virus replication, has a remarkable inhibiting effect on fish iridovirus replication at low concentration, and can be applied to preparation, research and development of fish antiviral drugs. Research of the antibacterial peptide has important scientific value and application value for further research and development of disease-resistant functional gene products.

The invention provides micropterus salmoides Hepcidin-1 (Ms-Hepcidin-1) and an application of a synthetic polypeptide of the micropterus salmoides Hepcidin-1 in preparation of an anti-fish virus drug. The research finds that the Ms-Hepcidin-1 synthetic polypeptide has a remarkable antiviral effect and is a novel antibacterial peptide with a function of inhibiting fish viruses. The Ms-Hepcidin-1 synthetic polypeptide and the recombinant eukaryotic expression vector of the Ms-Hepcidin-1 synthetic polypeptide can obviously reduce the transcription level of MCP, MMP and DNMT genes and the MCP protein level of LMBV, inhibit the replication of the LMBV and reduce the virus titer, and have an obvious fish virus resisting effect. Moreover, the polypeptide synthesized by Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use and convenient to preserve, can be used as a novel antiviral functional gene product to prepare antiviral drugs for fishes, and has important significance on prevention and control of fish iridovirus diseases.

The invention relates to a RAA-CRISPRCas13a method for detecting iridovirus fluorescence of micropterus salmoides, and relates to the technical field of biological detection.The method comprises the following steps that an RAA primer pair and guide crRNA are designed and synthesized based on a virus specific gene sequence; the method comprises the following steps: constructing a single-tube reaction system containing an RAA amplification component, Cas13a protein, crRNA and a fluorescent reporter molecule; after sample nucleic acid is added, constant-temperature incubation is carried out for 15-30 minutes at 37-42 DEG C, an RAA amplification product activates trans-cleavage activity of Cas13a, and a cleavage reporter molecule generates a fluorescence signal; and virus judgment is realized by detecting a fluorescence signal. According to the method, isothermal amplification and CRISPR detection are efficiently integrated through a single-tube reaction system, and the method has the advantages of being easy and convenient to operate, rapid in detection, high in sensitivity and high in specificity and is suitable for on-site high-throughput screening.

The invention belongs to the technical field of aquatic pathogen detection, and provides a primer and probe combination with strong specificity and high sensitivity, a kit and a cdPCR detection method in order to solve the problems of dependence of absolute quantification of an existing qPCR technology on a standard curve and low repeatability and stability caused by uncontrollable quality of a standard substance. The primer probe can generate specific amplification on LYCIV and has no cross reaction on other common aquatic pathogens (such as NNV, DIV1 and the like), so that the specificity of a detection result is fundamentally ensured, a false positive result caused by the cross reaction is effectively avoided, and the diagnosis accuracy is improved. The method has the characteristics of extremely high sensitivity and absolute quantification, the lower limit of detection reaches 6.2 copies / mu L, the method has more advantages in quantification of samples with extremely low concentration, early diagnosis and detection of extremely low virus load can be realized, viruses with extremely low content in fish bodies can be detected earlier, precious time is provided for disease early warning and early intervention, and the method is worthy of popularization and application. Disease outbreak is effectively prevented.

The invention provides an application of an Opnlrp12 gene in prevention and treatment of torus schlegeli virus and / or bacterial infection, and belongs to the technical field of gene engineering. Through targeted knockout of Opnlrp12, it is found that the expression level of a disease-resistant gene is also reduced, and therefore it is proved that Opnlrp12 can effectively inhibit infection caused by viruses and / or bacteria, and the survival rate of oplegnathus punctatus is increased. By means of the method, control over inflammatory diseases caused by iridovirus and / or vibrio harveyi can be achieved, and a target spot is provided for treatment of iridovirus infection of oplegnathus punctatus.

The application provides a preparation method of a shrimp iridovirus DIV1 neutralizing antibody and application thereof. The gene sequence shown as SEQ ID NO. 1 and the amino acid sequence shown as SEQ ID NO. 2 are used to solve the problem that there is no neutralizing antibody for the shrimp iridovirus DIV1 at present. The antibody for the shrimp iridovirus DIV1 prepared by the application can specifically recognize the DIVI virus and has good virus neutralizing activity, can effectively reduce the death of shrimps caused by DIV1 virus infection, significantly improve the ability of shrimps to resist DIV1 virus infection, and effectively prevent the infection and transmission of the virus.

This invention provides the application of Hepcidin-1 (Ms-Hepcidin-1) and its synthetic polypeptide in the preparation of antiviral drugs for largemouth bass. The invention reveals that the Ms-Hepcidin-1 synthetic polypeptide exhibits significant antiviral activity and is a novel antimicrobial peptide with the function of inhibiting fish viruses. Both the Ms-Hepcidin-1 synthetic polypeptide and its recombinant eukaryotic expression vector can significantly reduce the transcriptional levels of MCP, MMP, and DNMT genes and the MCP protein level of LMBV, inhibit LMBV replication, and reduce viral titers, demonstrating significant antiviral activity against fish viruses. Furthermore, the polypeptide synthesized from Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use, and easy to store. As a novel antiviral functional gene product, it can be applied to the preparation of antiviral drugs for fish, which is of great significance for the prevention and control of iridovirus disease in fish.

The invention relates to the technical field of gene detection, in particular to a rapid shrimp iridovirus detection primer group based on an RPA-CRISPR / Cas12a system, application and a kit. The primer group comprises an iridovirus constant-temperature gene amplification primer and a specific recognition amplification product target sequence probe; wherein the iridovirus constant-temperature gene amplification primer, the specific recognition amplification product target sequence CRISPR probe and the ssDNA reporter probe are recorded in the specification. An isothermal amplification primer and CrRNA are designed based on the shrimp red color virus gene, the target gene is amplified through the isothermal amplification technology, Cas12a is guided by the CrRNA to accurately recognize and activate trans-cleavage, a test strip report probe is synchronously split, and the result is interpreted by naked eyes within 30 min by virtue of the visual advantage of an immune test strip. The whole process does not need large instruments, the operation is simplified, the cost is low, and a visual and efficient solution is provided for on-site rapid diagnosis.

The invention relates to the field of material science and engineering, and particularly discloses a screening method of an anti-macrobrachium rosenbergii iridovirus natural compound based on an MCP protein target. The method comprises the following steps: constructing a three-dimensional structure of a virus major capsid protein MCP and determining an active pocket; performing structure pretreatment on the natural compound library; by taking the active pocket as a target spot, performing multiple rounds of virtual screening through molecular docking to obtain a candidate compound with binding energy less than or equal to-10.0 kcal / mol; and establishing a macrobrachium rosenbergii artificial infection model, determining the virus inhibition rate and protection rate of the candidate compound, and screening the active compound. The eugenol, the amentoflavone, the polyphyllin II and the solamargine screened by the method show a remarkable antiviral effect in an in-vivo injection test and breeding verification. According to the method, accurate screening from target spot prediction to in-vivo verification is realized.

The present application relates to a new application of radix cirsii japonici extract in resisting largemouth bass iridovirus. The present application finds that the radix cirsii japonici extract has a significant effect in resisting largemouth bass iridovirus, can significantly reduce the copy number of largemouth bass iridovirus, inhibit the expression of largemouth bass iridovirus MCP protein, improve the phagocytic ability of largemouth bass kidney leukocytes, and effectively improve the survival rate of fish infected with largemouth bass iridovirus, and has good biological safety. Therefore, the radix cirsii japonici extract can be used for preparing products such as drugs, fish feed or fish feed additives resisting largemouth bass iridovirus, and provides an effective prevention and control means for largemouth bass and other fish breeding.

This invention provides an application of a nucleic acid aptamer for recognizing grouper iridovirus and the aptamer itself. This application does not include disease diagnosis. The nucleic acid aptamer is a 50 bp nucleotide sequence, as shown in SEQ ID: 1. The nucleic acid aptamer for detecting grouper iridovirus includes the nucleotide sequence shown in SEQ ID: 1 or its derivatives. This invention utilizes SELEX technology to screen nucleic acid aptamers that exhibit high affinity and specificity for grouper iridovirus. Compared to existing protein antibodies, the nucleic acid aptamer provided by this invention has advantages such as high affinity, low cost, stable performance, ease of chemical synthesis, and ease of labeling. Therefore, when using the nucleic acid aptamer provided by this invention to detect grouper iridovirus, the operation is not only simple and rapid but also achieves high accuracy and sensitivity.

The invention discloses application of valproic acid in preparation of a medicine for resisting jewfish iridovirus. 24 mM of VPA is non-toxic to LJFin, and the effective use concentration of VPA for inhibiting SPIV infection in in-vitro cells is 3-24 mM. Through three administration modes of pretreatment, co-treatment and post-treatment, the VPA has the effects of preventing and treating SPIV infection. The VPA plays a role in resisting SPIV infection mainly by inhibiting entry and release of viruses, and has no obvious influence on adsorption of the viruses. In addition, after being fed as a feed additive, the feed additive does not affect ingestion and fish body health, and shows a good ability of enhancing the SPIV resistance of jewfish. According to the present invention, the death rate caused by SPIV infection is reduced by adding 0.25% and 0.5% of the VPA to the feed, the relative protection rates on SPIV are 21.1% and 31.6%, the virus replication in the tissue can be inhibited, and the virus-induced fish target tissue structure damage can be reduced; as an antiviral drug, VPA has a certain application value in prevention and control of jewfish iridovirus diseases.

The invention discloses application of a herba houttuyniae extract in preparation of a grouper iridovirus inhibitor. The herba houttuyniae extract is obtained by extracting a herba houttuyniae sample with water or an organic solvent. The herba houttuyniae extract prepared by the invention has a good inhibition effect on grouper iridovirus, does not generate drug resistance, has the characteristics of high efficiency, safety and no toxic or side effect, has a better antiviral effect compared with quercetin and isoquercitrin pure compounds, and has a wide application prospect in aquaculture.

The invention relates to a larimichthys crocea mariculture net cage and a larimichthys crocea iridovirus prevention and control method. The larimichthys crocea mariculture net cage comprises a net cage body, and a culture area is arranged in the net cage body; one end of the water inlet main pipe extends out of the culture area of the net cage body, and a medicine adding pipe and a fresh water adding pipe are arranged at the end, extending out of the culture area, of the water inlet main pipe; the water outlet pipe is arranged in the culture area of the net cage body, and a water outlet hole is formed in the bottom of the water outlet pipe; the water pushing pump is arranged between the water inlet main pipe and the water outlet pipe and is used for feeding water in the water inlet main pipe into the water outlet pipe; one end of the water inlet branch pipe is connected to the water inlet main pipe, the other end of the water inlet branch pipe is provided with an air inlet small pipe and a water inlet, the air inlet small pipe extends out of the culture area of the net cage body, and the water inlet is used for pumping water on the water surface. Deep water oxygenation of the culture area in the flat tide period is achieved, medicine and fresh water can be fed into the depth of the culture area, and prevention and control over the bodworm and the cryptocaryon irritans are achieved.

The invention specifically discloses an application of bacillus amyloliquefaciens E35 in preparation of a product for resisting grouper iridovirus infection, and the application of the bacillus amyloliquefaciens E35 in preparation of a product for resisting grouper iridovirus infection. The bacillus amyloliquefaciens E35 is preserved in Guangdong Microbial Culture Collection Center on December 20, 2024, and the preservation number is GDMCC 65657. According to the application of the bacillus amyloliquefaciens E35 in preparing the product for resisting grouper iridovirus infection, the effect of preventing and treating the grouper iridovirus is excellent, the infection activity of the grouper iridovirus on spleen cells is reduced, the death rate of groupers infected with the iridovirus is reduced, the disease time is delayed, and the safety of the groupers is improved. The drug resistance of fishes can be reduced, and toxic and side effects on fish bodies due to prevention and treatment can be reduced.

The invention discloses dsRNA (double-stranded ribonucleic acid) targeting a C3orf38 gene of litopenaeus vannamei and application of the dsRNA. According to the invention, dsRNA targeting the Litopenaeus vannamei C3orf38 gene (LvC3orf38) is designed and synthesized, and the dsRNA is used for inhibiting gene expression, so that it is found that inhibition of LvC3orf38 gene expression can inhibit duplication of the DIV1 in a prawn body, and the survival rate of the prawn infected with the DIV1 is improved. In addition, by inhibiting expression of the LvC3orf38 gene, weight gain of the prawns can be promoted, and growth of the prawns is promoted. Therefore, a preparation capable of inhibiting the expression of the LvC3orf38 gene can be used for improving the resistance of the prawns to the decapod iridovirus disease and promoting the growth of the prawns. A green and environment-friendly prevention and treatment strategy is provided for prevention and treatment of the full-eye iridovirus disease, the contradiction between disease resistance and growth can be solved, and the requirements of the modern breeding industry for high efficiency, safety and economy are met.

The application discloses a striped jack iridovirus inactivated vaccine and a preparation method thereof. The striped jack iridovirus SD strain virus liquid is used to prepare the striped jack iridovirus inactivated vaccine with high safety and good immunoprotective effect. The inactivated vaccine can effectively reduce the mortality of the striped jack after the striped jack is infected with the striped jack iridovirus, fills the blank of the inactivated vaccine for the striped jack iridovirus, and is helpful to the prevention and control of the striped jack iridovirus disease.

The invention belongs to the technical field of antivirus, and particularly discloses a siniperca chuatsi frog iridovirus oral composite vaccine and a preparation method thereof.The composite vaccine comprises an antigen core, a mucous membrane penetrating layer, a pH sensitive protective shell layer and a targeted modification layer, and the antigen core is formed by compounding a siniperca chuatsi frog iridovirus inactivated vaccine and an immunologic adjuvant; the mucous membrane penetrating layer is a pegylated chitosan-sodium alginate compound; the pH sensitive protective shell layer is a poly (beta-amino ester)-trehalose copolymer, and the targeted modification layer is wheat lectin. According to the siniperca chuatsi and frog iridovirus oral compound vaccine and the preparation method thereof, the technical problems that oral vaccines are easily degraded by digestive enzymes in aquatic animal bodies and the mucous membrane absorption efficiency is low are solved through the multi-layer structural design, the immune protection effect of the vaccines is remarkably improved, and the preparation method is simple and controllable and suitable for large-scale production.

The invention discloses application of a micropterus salmoides type 2 interferon related factor IFN-gamma rel in preparation of a medicine for treating and / or preventing iridovirus infection of micropterus salmoides. Through screening of transcriptome data after LMBV infection of micropterus salmoides and qPCR verification, expression of IFN gamma rel after LMBV infection is significantly up-regulated. The recombinant bacillus subtilis B.s-IFN-gammarel for expressing the protein is obtained by carrying out codon optimization on the gene of IFN gammarel, connecting the gene of IFN gammarel into plasmids to construct recombinant plasmids, and carrying out transformation and screening. Results show that the B.s-IFN-gammarel, as an antiviral oral drug for preventing or treating LMBV infection, can significantly improve the survival rate of micropterus salmoides, and has good safety. Therefore, the invention provides the application of IFN gammarel as an oral antiviral drug for preventing and treating LMBV infection in micropterus salmoides culture.

A cell culture medium for culturing of fish cells, a method for culturing fish cells, and an oral vaccine for fish. The cell culture medium for culturing of fish cells preferably comprises serum obtained from adult fish, and at a concentration of 0.2% to 2%. The oral vaccine may comprise an inactivated iridovirus.

The application discloses a gold tiger kidney cell, a method for establishing the same and an application thereof, and belongs to the technical field of cell culture. The gold tiger kidney cell is preserved in the China General Microbiological Culture Collection Center on April 3, 2025, and the preservation number is CGMCC No.46341. The gold tiger kidney cell can be stably proliferated for more than 70 generations, has a high proliferation speed, is stable in morphology, is sensitive to iridovirus and common pathogenic bacteria in aquaculture, and can be used to establish an iridovirus or bacterial infection cell model, thereby laying a foundation for researching an iridovirus infection mechanism, a bacterial infection mechanism, preparing an iridovirus vaccine or a therapeutic drug, and a bacterial infection therapeutic drug.

The invention discloses a triple RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction) method and a kit for simultaneously detecting IMNV (Immunodeficiency Virus), CMNV (Cytomegalovirus) and DIV1 (Deoxyribose Immunodeficiency Virus According to the invention, PCR primers and TaqMan probes for detecting IMNV, CMNV and DIV1 are respectively designed, and the nucleotide sequences of the PCR primers and TaqMan probes are shown as SEQ ID NO: 1-9. The primer and the probe can be used for simultaneously qualitatively and quantitatively detecting the infectious muscle necrosis virus, the secretly death nodavirus and the full-eye iridovirus 1 through a triple RT-qPCR (Reverse Transcription-Quantitative Polymerase Chain Reaction) method. According to the invention, common detection of RNA virus and DNA virus is realized for the first time, the kit has the characteristics of high efficiency and convenience, and the advantages of high sensitivity, high specificity and quantitability, and meanwhile, due to the addition of the molecular crowding agent, the detection efficiency and detection speed of medium and low-concentration virus load can be effectively improved, and the detection cost is reduced. The invention provides a convenient and reliable detection method and product for early monitoring, early warning and diagnosis of the three virus diseases of the penaeus vannamei boone.

This invention provides a largemouth bass iridovirus, its prepared vaccine, and its applications, belonging to the field of biological vaccine technology. The largemouth bass iridovirus LMBV-ZJDSS-F110 provided by this invention is deposited at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, on September 20, 2024, with accession number CCTCC NO: V202485. This strain and the vaccine prepared from it can effectively prevent largemouth bass iridovirus infection and provide good protection against largemouth bass iridovirus disease.

The application discloses a scylla paramamosain antibacterial peptide Sparamosin 26‑54 The application has significant antiviral effect on white spot syndrome virus, frog iridovirus and eel herpesvirus, has the characteristics of easy synthesis, stability, high antiviral activity and low toxicity, and has good application prospect in drug research.

This invention discloses the application of nano-zinc oxide in the preparation of drugs against fish iridovirus (SGIV). The study shows that nano-zinc oxide (ZnONPs) can significantly attenuate the cytopathic effect induced by SGIV infection, reducing the mRNA transcription level, protein expression level, and viral titer of the major SGIV capsid protein. Treatment with ZnONPs can also enhance the host's interferon immune-related genes ISG56 and ISG20, and anti-inflammatory factors IL-10 and TGF-β. β The ZnONPs significantly reduced the mRNA transcription level, thereby decreasing the infectivity of SGIV to cells and demonstrating antiviral activity against fish iridoviruses. Furthermore, ZnONPs exhibited low cytotoxicity and good safety profile, making them suitable for the preparation of drugs to prevent and treat grouper iridovirus disease. This invention provides a novel drug intervention strategy for the prevention and treatment of SGIV infection and has significant application value in the control of iridovirus-related diseases in aquaculture.

The invention discloses a synchronous rapid detection method for macrobrachium rosenbergii iridovirus and multiple pathogens, and belongs to the technical field of biology. According to the method, macrobrachium rosenbergii tissue gene DNA is extracted by adopting a Chelex method, then PCR amplification is performed, and an amplification product is detected for judgment. According to the method, the genome DNA is extracted by adopting an optimized CHELEX method, enough DNA samples for detection can be extracted only by using a trace amount of tissue samples, meanwhile, the DNA extraction time is greatly shortened, and only 20 minutes are needed from sample treatment to extraction of enough macrobrachium rosenbergii tissue DNA. PCR (Polymerase Chain Reaction) specific amplification and gel electrophoresis are carried out by taking the macrobrachium rosenbergii as a template, so that the aim of quickly detecting common diseases (especially iridovirus) of macrobrachium rosenbergii can be fulfilled.

The invention discloses a prawn culture pathogenic bacteria multiple ERA detection primer and probe based on a biochip technology and application of the prawn culture pathogenic bacteria multiple ERA detection primer and probe. The kit is characterized in that the primers and the probe respectively aim at tcdB genes of highly pathogenic vibrios, hypothetical protein genes of white spot syndrome viruses, 16S rRNA genes of enterocytozoon hepatopenaei, non-structural protein genes of infectious subcutaneous and hematopoietic necrosis viruses, DNA primer enzyme genes of decapod iridovirus 1 and PirB genes of bacterial prawn acute hepatopancreatic necrosis pathogens; the method comprises the following steps of: detecting a capsid protein gene of a prawn infectious muscle necrosis virus, a capsid protein gene of a nedavirus, a capsid protein gene of the nedavirus, a structural protein ORF1 gene of the prawn infectious muscle necrosis virus, a non-structural polyprotein gene of a taura syndrome virus and a Rep-1b gene of a yellow head virus, and further discloses specific sequences. The sensitivity, the specificity and the accuracy are high.

The application discloses a Plectropomus leopardus spleen cell line and application thereof. Plectropomus leopardus The spleen cell line PLS has a preservation number of GDMCC No: 68131. The PLS is obtained through primary culture and multiple subculture, has a fast cell growth speed, has been stably subcultured for more than 60 generations, has good activity after cryopreservation, and lays a foundation for Plectropomus leopardus gene germplasm resource preservation. Meanwhile, the PLS is sensitive to fish nervous necrosis virus and iridovirus, can be applied to exogenous gene function research, fish virus isolation, virus infection pathogenesis, virus host interaction and virus in-vitro infection model, and provides a high-efficiency amplification system for Plectropomus leopardus virus vaccine preparation.