137 results about "Iridovirus" patented technology

The invention discloses a macrobrachium rosenbergii iridovirus disease resistance-related SNP (Single Nucleotide Polymorphism) site, a site combination and application thereof, and belongs to the field of aquatic organism breeding. According to the invention, SNP loci related to resistance of the macrobrachium rosenbergii iridovirus disease are successfully screened through whole genome association analysis, and the SNP loci are respectively located at a 9574545th base of a chromosome 10 of the macrobrachium rosenbergii and a 26491028th base of a chromosome 14 of the macrobrachium rosenbergii. Aiming at the two SNP sites, an SNP site combination related to the resistance of the macrobrachium rosenbergii iridovirus disease is further developed, and macrobrachium rosenbergii iridovirus disease resistant individuals can be screened by detecting the SNP site combination. Therefore, the SNP molecular marker provided by the invention can be used for macrobrachium rosenbergii molecular marker assisted breeding, accelerates disease-resistant macrobrachium rosenbergii variety breeding, and provides a reference basis for disease-resistant breeding work of macrobrachium rosenbergii.

The invention provides a targeted giant salamander iridovirus nano antibody as well as a preparation method and application thereof. The invention discloses a giant salamander iridovirus targeting nano antibody, and aims to provide a giant salamander iridovirus targeting nano antibody which is low in expression cost, low in difficulty and stable in source, can be effectively combined with giant salamander iridovirus, and specifically realizes immunoassay of the giant salamander iridovirus. The amino acid sequences of the nano antibody are shown as SEQ ID NO.1-6, and the nano antibody belongs to the technical field of biological science.

The invention discloses paenibacillus kribbensis exopolysaccharide and application thereof in prevention and control of iridovirus diseases of micropterus salmoides. The invention provides a paenibacillus kribbensis exopolysaccharide. The paenibacillus kribbensis exopolysaccharide is prepared from a fermentation product of a paenibacillus kribbensis space mutation delta PS04-17 strain. As a fish feed additive, the paenibacillus kribbensis exopolysaccharide has better in-vivo safety, has a promoting effect on the growth of the largemouth bass, and can promote the growth and weight gain of the largemouth bass and reduce the feed coefficient of the largemouth bass. Meanwhile, as a drug for resisting LMBV virus infection, the compound can inhibit expression of viruses in target tissues, alleviate virus-induced fish body target tissue structure damage and reduce the death rate caused by virus infection. Besides, the paenibacillus kribbensis exopolysaccharide serving as a disease-resistant immunopotentiator can maintain intestinal health of the micropterus salmoides, is simple to prepare, low in cost and easy to obtain in quantity, and provides more products and methods for prevention and control of the iridovirus disease of the micropterus salmoides.

The invention relates to the field of aquaculture, in particular to a preparation method and application of a larimichthys crocea iridovirus recombinant live vector vaccine. According to the invention, replication-deficient type 5 adenovirus is selected as a target gene expression vector, a recombinant adenovirus vector comprising main capsid protein of iridovirus is constructed, recombinant virus is obtained, and the safe and effective large yellow croaker iridovirus disease recombinant live vector vaccine is created. According to the vaccine, the larimichthys crocea is immunized in a soaking or bait feeding mode, the CPM of the larimichthys crocea immunized by the vaccine is 12.0%, and the CPM of the fish without being immunized by the vaccine is 62.0%. In addition, the RPS of the inoculated fish and the RPS of the empty carrier control group fish are 80.65% and 9.68% respectively. The method is at a leading level in the field of pseudosciaena crocea biomedicine at present, and creativity is remarkable.

A spore of a bacillus subtilis B.subtilis WB600 strain is used as an antigen delivery carrier, spore coat protein C (CotC) is used as anchoring protein, a key protein ATPase gene and a CotC gene of micropterus salmoides iridovirus are fused through a gene fusion technology, a recombinant spore surface display system is constructed, the ATPase antigen is stably displayed on the spore surface, and an oral vaccine is prepared. After being orally applied to fishes, the vaccine can tolerate the gastrointestinal tract environment and is accurately delivered to intestinal related lymphatic tissues to stimulate fish immunity, and finally prevention and control of the iridovirus of the micropterus salmoides are achieved.

The invention provides a composition and a kit for detecting lymphocyst virus based on RPA isothermal amplification and immunochromatography and application, and relates to the technical field of biological detection, the composition comprises an upstream primer MCP-F with a nucleotide sequence as shown in SEQ ID NO: 7, a downstream primer MCP-R with a nucleotide sequence as shown in SEQ ID NO: 8 and a specific probe MCP-P with a nucleotide sequence as shown in SEQ ID NO: 9, according to the present invention, with reference to the LCDV strain genome sequence, the MCP conserved gene sequence of the LCDV strain is determined, and the RPA primer and the specific probe are designed, such that the lymphocystis virus, the largemouth bass frog iridovirus, the viral hemorrhagic septicemia virus, the infectious hematopoietic necrosis virus, the paralichthys olivaceus rhabdovirus, the flounder nested virus and the lymphocystis virus have specificity; the sensitivity reaches 5pg / reaction; and the amplification product is suitable for the LFD test strip.

The invention provides a larimichthys crocea iridovirus inactivated vaccine as well as a preparation method and application thereof, and belongs to the technical field of preparation of viral vaccines. The larimichthys crocea iridovirus inactivated vaccine comprises an inactivated larimichthys crocea iridovirus and an adjuvant, and the strain of the larimichthys crocea iridovirus is an FD201807 strain. After the larimichthys crocea iridovirus inactivated vaccine is injected, the phenomena of ingestion and abnormal behaviors of fishes are avoided, the death phenomenon is avoided, the safety is high, the relative immune protection rate of the inactivated vaccine to immunized fishes is 51.20% or above, the immune effect is good, and the larimichthys crocea iridovirus inactivated vaccine can be applied to prevention and treatment of larimichthys crocea iridovirus diseases. The preparation method of the larimichthys crocea iridovirus inactivated vaccine is simple and convenient, easy to operate, low in cost and suitable for industrial production.

The invention discloses a multiplex fluorescent quantitative PCR (polymerase chain reaction) detection kit for prawn culture pathogenic bacteria, which is characterized in that the kit contains primers and probes for detecting the prawn culture pathogenic bacteria, wherein the primers and the probes are respectively used for detecting TDH-related hemolsin genes of vibrio parahaemolyticus, ORF107 genes of white spot syndrome viruses, small subunit ribosomal genes of shrimp enterocytozoon hepatopenaei, 37 kDa coat protein genes of infectious subcutaneous and hematopoietic necrosis viruses, DNA (deoxyribonucleic acid) primer genes of full-eye iridovirus 1 and PirA [pi] genes of pathogens of bacterial prawns acute hepatopancreas necrosis; the method has the advantage that the detection of eight prawn culture pathogens can be realized at the same time. The method comprises the following steps of: obtaining a tcdB gene of a high-pathogenicity vibrio and a hemolsin gene of a vibrio harveyi;

The invention discloses a primer probe composition for detecting prawn disease related pathogens, a kit and application of the primer probe composition. The primer probe composition comprises a first primer probe group used for detecting vibrio parahaemolyticus, a second primer probe group used for detecting a tetrapod iridovirus 1, a third primer probe group used for detecting vibrio vitriocultatus and a fourth primer probe group used for detecting enterocytozoon hepatopenaei. The primer probe composition has the characteristics of high sensitivity, good repeatability and strong specificity, and can be used for specifically detecting vibrio parahaemolyticus, tetrapod iridovirus 1, vibrio vitrioculi and enterocytozoon hepatopenaei.

The invention discloses a recombinant yeast for expressing large yellow croaker iridovirus MCP protein and a preparation method and application of the recombinant yeast in preparation of vaccines, and is characterized in that the recombinant yeast is saccharomyces cerevisiae transformed with pYD1-MCP recombinant plasmids and is named as EBY100 / pYD1-MCP, the pYD1-MCP recombinant plasmids can express the MCP protein on a yeast surface display system, the nucleotide sequence of a gene segment of the MCP protein is shown as SEQ ID NO.1, the nucleotide sequence of the gene segment of the MCP protein is shown as SEQ ID NO.2, and the nucleotide sequence of the gene segment of the MCP protein is shown as SEQ ID NO.2. The preparation method comprises a step of constructing a recombinant plasmid pYD1-MCP and a step of constructing a recombinant yeast EBY100 / pYD1-MCP, the oral vaccine of the larimichthys crocea iridovirus vaccine can be prepared, and the oral vaccine has the advantages of being high in protection rate, safe, low in cost, convenient to use and good in application prospect in prevention and control of the larimichthys crocea iridovirus.

The invention discloses a polyclonal antibody of a grouper iridovirus SGIV VP101 gene and application of the polyclonal antibody. The polyclonal antibody is prepared from an SGIVVP101 gene, and the nucleotide sequence of the VP101 gene of an optimized codon is as shown in SEQ ID NO. 1. The polyclonal antibody of the SGIV VP101 gene recombinant expression protein prepared by the invention has good specificity, not only can specifically recognize a prokaryotic expression product of the SGIV VP101, but also can be used for quantitative and positioning analysis of the SGIV VP101 in vivo and in vitro. The successful preparation of the polyclonal antibody of the recombinant expression protein of the SGIV VP101 gene lays a foundation for the establishment of an SGIV immunological detection method, the quantitative and positioning analysis of the protein of the VP101 gene and the analysis of gene functions.

The invention relates to a multiplex PCR (polymerase chain reaction) primer group for simultaneously detecting four pathogens, a detection method and application, and belongs to the field of molecular biology, the four pathogens are nervous necrosis virus, iridovirus, streptococcus iniae and vibrio harveyi, and the primer group is composed of SEQ ID NO.1-8. By utilizing the primer, nervous necrosis virus, iridovirus, streptococcus iniae and vibrio harveyi can be simultaneously detected in a short time, and the aim of establishing a simple and effective method for simultaneously detecting various fish pathogens is fulfilled. And the detection primer group and the detection method provided by the invention have good specificity and relatively high sensitivity.

The invention belongs to the technical field of biology, discloses application of naringenin in resisting siniperca chuatsi and frog iridovirus, and discovers the antiviral activity of the naringenin to the siniperca chuatsi and frog iridovirus, the naringenin can down-regulate the expression level of NF-kB and MAPK pathway genes triggered by MRV, effectively inhibit MRV-induced excessive inflammatory response and inhibit MRV proliferation, and the application of the naringenin in resisting the siniperca chuatsi and frog iridovirus. In addition, tissue damage caused by MRV infection can be relieved, and the survival rate of MRV infected fishes is effectively increased; a foundation is provided for preparing a product for resisting the siniperca chuatsi and frog iridovirus by adopting naringenin; meanwhile, the invention also discloses a drug containing naringenin, a fish feed and a fish feed additive.

The invention belongs to the technical field of biology, and discloses application of wogonin in resisting siniperca chuatsi and frog iridovirus, the wogonin can effectively inhibit over-generation of ROS caused by MRV virus infection, and oxidative stress damage of host cells is relieved in a dose-dependent mode. The wogonin can be used for preventing and treating the siniperca chuatsi and frog iridovirus, the high expression state of a proinflammatory gene can be reduced, intestinal tissue damage induced by MRV infection can be relieved, proliferation of MRV in the micropterus salmoides is effectively inhibited, the MRV challenge survival rate of the micropterus salmoides is increased, a basis is provided for preparing a product for resisting the siniperca chuatsi and frog iridovirus from wogonin, and the wogonin has important application value. Meanwhile, the invention also discloses a medicine, a fish feed and a fish feed additive of the wogonin.

The invention relates to the technical field of animal virology, in particular to a target sequence for detecting the virulence of a largemouth bass frog iridovirus and application of the target sequence. The invention provides a target sequence for detecting the virulence of a largemouth bass frog iridovirus. The nucleotide sequence of the target sequence is as shown in SEQ ID NO: 1; the invention also provides a primer pair for detecting the virulence of the largemouth bass frog iridovirus. The detection method provided by the invention can efficiently and accurately distinguish the virulent strain and the virulent strain of the largemouth bass frog iridovirus, the detection time can be completed within 1.5 hours, a powerful support is provided for accurate judgment of the virulence of the largemouth bass frog iridovirus, and an effective means is also provided for screening the virulent strain and the virulent strain of the largemouth bass frog iridovirus.

The invention provides a largemouth bass frog iridovirus, a vaccine prepared from the largemouth bass frog iridovirus and application of the largemouth bass frog iridovirus, and belongs to the technical field of biological The largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth largemouth The strain and the vaccine prepared from the strain can effectively prevent attack of the largemouth bass frog iridovirus, and have relatively good protection force on the largemouth bass frog iridovirus disease.

The invention discloses a targeted delivery system of a largemouth bass frog iridovirus oral vaccine and a preparation method of the targeted delivery system, and relates to the technical field of biological medicines.The method comprises the following steps that a starch solution and a largemouth bass frog iridovirus inactivated vaccine are stirred and mixed, then a paraffin emulsion is added, stirring and emulsifying are conducted, and a W / O emulsion is obtained; adding calcium chloride, stirring and mixing, centrifuging, and resuspending by using a PBS (Phosphate Buffer Solution) to obtain a microsphere resuspension solution; stirring and mixing with a quaternized chitosan solution, adding glutaraldehyde for crosslinking, centrifuging, and freeze-drying. The invention further discloses a targeted delivery system of the largemouth bass frog iridovirus oral vaccine prepared by the method. The vaccine is orally administered after being microencapsulated, so that the human cost is low, the stimulation to fish bodies is small, the problems of inconvenience in fish administration, low bioavailability of oral administration, instability in gastrointestinal tracts and the like are solved, and the vaccine has an excellent application prospect in the field of LMBV prevention and control.

The invention discloses an application of bortezomib in preparation of a medicine for resisting grouper iridovirus. Researches show that bortezomib has a remarkable inhibiting effect on grouper iridovirus, and is low in cytotoxicity and good in safety. The bortezomib can obviously reduce the fluorescence signal intensity of virus protein, inhibit the transcriptional level of main capsid protein MCP and envelope protein VP19 of the virus and reduce the copy number of DNA and mRNA of the virus; the bortezomib can effectively inhibit grouper iridovirus infection and effectively block synthesis of virus protein, so that efficient inhibition of grouper iridovirus infection is achieved, and along with prolonging of virus infection time, bortezomib can also remarkably inhibit virus gene transcription, protein synthesis and genome replication. Therefore, bortezomib not only can effectively prevent and treat grouper iridovirus infection, but also has the characteristics of high specificity, low toxicity, remarkable antiviral effect and the like, and also has important application value in prevention and control of iridovirus-related diseases in aquaculture industry.

The invention discloses an application of Micropterus salmoides Piscidin polypeptide in preparation of an anti-fish virus drug. The amino acid sequence of the polypeptide is as shown in SEQ ID NO. 2 or SEQ ID NO. 3. The research finds that the micropterus salmoides Piscidin is an antibacterial peptide with an antiviral function, the polypeptide synthesized by the artificially synthesized micropterus salmoides Piscidin has the characteristic of inhibiting fish DNA virus replication, has a remarkable inhibiting effect on fish iridovirus replication at low concentration, and can be applied to preparation, research and development of fish antiviral drugs. Research of the antibacterial peptide has important scientific value and application value for further research and development of disease-resistant functional gene products.

The invention relates to a rapid detection method for nucleic acid of iridovirus of Micropterus salmoides and a kit thereof, belonging to the technical field of molecular biology, the rapid detection method adopts a two-step method of RPA-Cas12a for treatment, can be used for fluorescence detection through fluorescence labeling and also can be used for detection through an immune colloidal gold test strip, and is simple and convenient to operate, rapid and sensitive, strong in specificity, good in stability and low in cost.

The invention provides micropterus salmoides Hepcidin-1 (Ms-Hepcidin-1) and an application of a synthetic polypeptide of the micropterus salmoides Hepcidin-1 in preparation of an anti-fish virus drug. The research finds that the Ms-Hepcidin-1 synthetic polypeptide has a remarkable antiviral effect and is a novel antibacterial peptide with a function of inhibiting fish viruses. The Ms-Hepcidin-1 synthetic polypeptide and the recombinant eukaryotic expression vector of the Ms-Hepcidin-1 synthetic polypeptide can obviously reduce the transcription level of MCP, MMP and DNMT genes and the MCP protein level of LMBV, inhibit the replication of the LMBV and reduce the virus titer, and have an obvious fish virus resisting effect. Moreover, the polypeptide synthesized by Ms-Hepcidin-1 is simple to prepare, low in cost, safe to use and convenient to preserve, can be used as a novel antiviral functional gene product to prepare antiviral drugs for fishes, and has important significance on prevention and control of fish iridovirus diseases.

The invention discloses a mandarin fish medullary tissue cell line and a construction method and application thereof. The invention belongs to the technical field of aquatic organism cells, and discloses a mandarin fish medulla oblongata cell line MFSB, which is preserved in China Center for Type Culture Collection on December 4, 2024, has a preservation number of CCTCC NO: C2024376, and is named as mandarin fish medulla oblongata cell line MFSB. The construction method comprises the steps of primary culture of mandarin fish medulla oblongata tissue cells, subculture, cryopreservation and resuscitation of the cells and the like, and the mandarin fish medulla oblongata cell line MFSB has the advantages of stable subculture, strong cell line division ability, fast growth speed and good stability, can be used as a cell model or an experimental tool to be applied to scientific research and animal genetic breeding, and has wide application prospects. The probe has broad-spectrum sensitivity to mandarin fish infectious spleen and kidney necrosis virus, mandarin fish double-segment RNA virus, mandarin fish frog iridovirus and mandarin fish rhabdovirus, and can be well applied to separation, culture and detection of mandarin fish virus and research and development of vaccines.

The application discloses an anti-LMBV single-chain antibody and application thereof in preparation of a quantum dot fluorescent immunochromatography test strip, and belongs to the technical field of biological detection. The single-chain antibody comprises Ab-K1 and Ab-K2, the amino acid sequence of the Ab-K1 is shown as SEQ ID NO. 11, and the amino acid sequence of the Ab-K2 is shown as SEQ ID NO. 12. The single-chain antibody used in the application can accurately recognize and combine with iridovirus in a sample to be detected, so that the accuracy of detection is ensured. Meanwhile, the antibody labeled with fluorescent quantum dots has good fluorescent characteristics, so that the fluorescent signal is easy to observe and detect, and the specific surface area is relatively large, so that the antibody can be combined with more virus molecules, so that the sensitivity of detection is improved, the accuracy and sensitivity of detection are improved, the operation steps are simplified, and powerful technical support is provided for detection and prevention and control of iridovirus.

The invention relates to a RAA-CRISPRCas13a method for detecting iridovirus fluorescence of micropterus salmoides, and relates to the technical field of biological detection.The method comprises the following steps that an RAA primer pair and guide crRNA are designed and synthesized based on a virus specific gene sequence; the method comprises the following steps: constructing a single-tube reaction system containing an RAA amplification component, Cas13a protein, crRNA and a fluorescent reporter molecule; after sample nucleic acid is added, constant-temperature incubation is carried out for 15-30 minutes at 37-42 DEG C, an RAA amplification product activates trans-cleavage activity of Cas13a, and a cleavage reporter molecule generates a fluorescence signal; and virus judgment is realized by detecting a fluorescence signal. According to the method, isothermal amplification and CRISPR detection are efficiently integrated through a single-tube reaction system, and the method has the advantages of being easy and convenient to operate, rapid in detection, high in sensitivity and high in specificity and is suitable for on-site high-throughput screening.

The invention relates to the technical field of animal virology, in particular to a mutant gene and application thereof, and a primer pair for detecting virulence of a largemouth bass frog iridovirus and application of the primer pair. The invention provides a mutant gene. The sequence of the mutant gene is as shown in SEQ ID NO: 1. The invention also provides a primer pair and a detection method for detecting the virulence of the micropterus salmoides iridovirus, the detection method can quickly and accurately identify whether the micropterus salmoides iridovirus is a virulent strain or a low virulent strain, and the detection method is simple and convenient to operate.

The invention belongs to the technical field of aquatic pathogen detection, and provides a primer and probe combination with strong specificity and high sensitivity, a kit and a cdPCR detection method in order to solve the problems of dependence of absolute quantification of an existing qPCR technology on a standard curve and low repeatability and stability caused by uncontrollable quality of a standard substance. The primer probe can generate specific amplification on LYCIV and has no cross reaction on other common aquatic pathogens (such as NNV, DIV1 and the like), so that the specificity of a detection result is fundamentally ensured, a false positive result caused by the cross reaction is effectively avoided, and the diagnosis accuracy is improved. The method has the characteristics of extremely high sensitivity and absolute quantification, the lower limit of detection reaches 6.2 copies / mu L, the method has more advantages in quantification of samples with extremely low concentration, early diagnosis and detection of extremely low virus load can be realized, viruses with extremely low content in fish bodies can be detected earlier, precious time is provided for disease early warning and early intervention, and the method is worthy of popularization and application. Disease outbreak is effectively prevented.

The invention provides an application of an Opnlrp12 gene in prevention and treatment of torus schlegeli virus and / or bacterial infection, and belongs to the technical field of gene engineering. Through targeted knockout of Opnlrp12, it is found that the expression level of a disease-resistant gene is also reduced, and therefore it is proved that Opnlrp12 can effectively inhibit infection caused by viruses and / or bacteria, and the survival rate of oplegnathus punctatus is increased. By means of the method, control over inflammatory diseases caused by iridovirus and / or vibrio harveyi can be achieved, and a target spot is provided for treatment of iridovirus infection of oplegnathus punctatus.

The application provides a preparation method of a shrimp iridovirus DIV1 neutralizing antibody and application thereof. The gene sequence shown as SEQ ID NO. 1 and the amino acid sequence shown as SEQ ID NO. 2 are used to solve the problem that there is no neutralizing antibody for the shrimp iridovirus DIV1 at present. The antibody for the shrimp iridovirus DIV1 prepared by the application can specifically recognize the DIVI virus and has good virus neutralizing activity, can effectively reduce the death of shrimps caused by DIV1 virus infection, significantly improve the ability of shrimps to resist DIV1 virus infection, and effectively prevent the infection and transmission of the virus.

The invention discloses an application of alpha-lipoic acid in preparation of an anti-iridovirus drug. Related experiments show that alpha-lipoic acid can safely and effectively treat iridovirus such as frog virus type 3, not only can effectively reduce harm of iridovirus to frog and fish culture, but also can promote ecological high-quality development of aquaculture industry, and has obvious economic and social benefits.

The invention provides a method for identifying the iridovirus damage grade of micropterus salmoides based on machine vision assistance, and belongs to the technical field of aquatic product infection identification. According to the method, the damage degree of micropterus salmoides infected with iridovirus is measured in a standardized mode, early-stage micropterus salmoides body surface damage data are analyzed based on visual machine assistance, body surface damage areas are divided, the body surface damage number and damage area are measured, individual comprehensive damage scores are calculated through data standardization and PCA dimension reduction, and body surface damage grades are constructed. And a scientific basis is provided for disease management of the micropterus salmoides.