564 results about "Phosphopeptide" patented technology

A bioactive complex composition having enhanced oxidative stability, emulsion stability, mineral rich transparent beverages and a wide range of functional health benefits. The composition may include as a base composition individual ingredients or a synergistic blend of mineral salts, Omega-3 rich oils, phospholipids, chitosan, and alpha-casein, beta-casein, kappa-casein or protein fragments, glycopeptides, phosphopeptides. The composition may optionally be further utilized for the prevention of hypercholesterolemia, bone (and teeth) mineral loss, treatment of mental health diseases, heart health, additional nutritional supplementation, and treatment of additional medical conditions.

An antioxidant composition having enhanced oxidative stability, emulsion stability, and health benefits. The composition may include individual ingredients or a synergistic blend of non-reducing sugars, sugar polyols, medium-chain triglycerides, polysaccharides, polyphenols, phospholipids, chitosan, and alpha-casein, beta-casein, kappa-casein or protein fragments, glycopeptides, phosphopeptides. The composition may optionally be further utilized for the prevention of hypercholesterolemia or bone mineral loss.

The invention relates to infant formula milk powder and a production method thereof. The infant formula milk powder contains nutrition constituents such as protein, fat, carbohydrate, vitamins, mineral elements and the like with content regulated by the national standards on infant foods. Moreover, whey peptides, collagen peptides and casein phosphopeptides are added in the milk powder. Since the three kinds of binding peptides are added, the invention has the advantages that the immunity of cesarean infants is improved and the disease resistance of the infants is enhanced; the digestive function of the infants is improved, the intestinal mucosa proliferation is promoted and the digestive function is improved; and the development of the nervous system is promoted, the nutrition supply to the nervous system is guaranteed and the memory and the learning ability of the infants are improved.

The invention discloses a formula milk powder for promoting the absorption of fatty acid and calcium and a preparation method thereof. Raw cow milk, lactose, 1,3-Dioleoyl 2-palmitoyl triglyceride and demineralized whey powder as main materials are added with concentrated whey albumen powder, Alpha-lactalbumin powder, oligosaccharide, walnut oil, casein phosphopeptide, docosahexaenoic acid, arachidonic acid, nucleotide, lutein, inositol, carnitine and the like as well as vitamins, mineral substances and other nutrients needed for strengthening infants, and fat humanization, protein humanization and carbohydrate humanization are realized. The powdery product is produced by the processes of blending, homogenization, concentration, spray-drying, packaging and the like. According to the physiological characteristics and nutritional demand of the infants, the invention reinforces the calcium, the 1,3-Dioleoyl 2-palmitoyl triglyceride, other nutrient ingredients and the like, and aiming at the oversea clinical test conclusion of the 1,3-Dioleoyl 2-palmitoyl triglyceride, the final test conclusion of comparison with breast milk and infant formula milk powder sold on the market in the process of a clinical feeding test is that the feeding result of the designed formula approximates the feeding result of the breast milk and is better than the feeding result of an infant formula milk powder group sold on the market.

A nanoemulsion composition having enhanced oxidative stability, emulsion stability, and health benefits. The composition may include individual ingredients or a synergistic blend of non-reducing sugars, sugar polyols, medium-chain triglycerides, polysaccharides, polyphenols, phospholipids, chitosan, and alpha-casein, beta-casein, kappa-casein or protein fragments, glycopeptides, phosphopeptides. The composition may optionally be further utilized for the prevention of hypercholesterolemia or bone (and teeth) mineral loss.

The invention provides a calcium supplementing and calcium locking health care food. The calcium supplementing and calcium locking health care food comprises calcium, colostrum basic protein, chondroitin sulfate, D-glucosamine, vitamin D, casein phosphopeptides and the like, wherein the colostrum basic protein is obtained by degreasing, separating, concentrating, removing casein, alpha-lactoalbumin, beta-lactoglobulin and substances with molecular weights of more than 30 kDa and less than 1 kDa and drying at a low temperature; the biggest difference between the calcium supplementing and calcium locking health care food prepared by the method provided by the invention and the prior art is that the capacity of the bone tissue in actively absorbing osteoblast nutrition like calcium, ossein and the like can be effectively improved and effects of activating osteoblasts and inhibiting osteoclast activity to enhance bone mineral density and length and promote the health of the bone are obtained. The calcium supplementing and calcium locking health care food provided by the invention, which is a latest generation of domestic osteoarthrosis rehabilitation product, obtains breakthrough in the calcium supplementing effect, can be also used for helping the bone to preferably absorb the calcium in the food, has no toxic and side effect after being taken for a long time.

The invention discloses a composition for reducing bone loss and a preparation method thereof. The composition is prepared from a calcium compound, marine fishbone collagen oligopeptide powder, glucosamine hydrochloride, casein phosphopeptides, natural vitamin E, vitamin C, vitamin K1, vitamin B12, vitamin D3 and dietary fiber. As proved by animal and human body tests, the composition has the effects of increasing bone density, enhancing bone intensity, reducing bone loss, delaying aging and enhancing the immunity, and can be taken by the middle-aged and old as a health-care food for a long time. The preparation method is scientific and reasonable, and is suitable for industrial production.

The invention belongs to the technical field of functional material and particularly relates to a magnetic mesoporous titanium dioxide core-shell type compound microsphere as well as a preparation method and applications thereof. The core-shell type compound microsphere takes a ferroferric oxide nano particle cluster as the core and takes mesoporous titanium dioxide with high crystallinity as the shell. The preparation method comprises the following steps: firstly, preparing the ferroferric oxide nano particle cluster with stable citric acid, secondly, wrapping the surface of the cluster with an amorphous titanium dioxide shell through a sol-gel method, and finally, obtaining the magnetic mesoporous titanium dioxide core-shell type compound microsphere through hydro-thermal treatment. By being provided with the mesoporous titanium dioxide shell with high crystallinity, the compound microsphere has the characteristics of high selectivity, high enrichment capacity, high sensitivity and high recovery rate to phosphoeptide; and since a magnetic core exists, the microsphere enriched in phosphoeptide can be separated by magnetism quickly and very conveniently. The method provided by the invention has the advantages that the operation is simple, the process is controllable, and the prepared compound microsphere can be used for analyzing and identifying phosphoeptide with ultra low concentration in a biological sample.

A method for selective labeling of phosphate groups in natural and synthetic oligomers and polymers in the presence of chemically related groups such as carboxylic acid groups. The method is specifically applicable to biological oligomers and polymers, including phosphopeptides, phosphoproteins and phospholipids. In a specific embodiment, selective labeling of phosphate groups in proteins and peptides, for example, facilitates separation, isolation and detection of phosphoproteins and phosphopeptides in complex mixtures of proteins. Selective labeling can be employed to selectively introduce phosphate labels at phosphate groups in an oligomer or polymer, e.g., in a peptide or protein. Detection of the presence of the label, is used to detect the presence of the phosphate group in the oligomer or polymer. The method is useful for the detection of phosphoproteins or phosphopeptides. The phosphate label can be a colorimetric label, a radiolabel, a fluorescent or phosphorescent label, an affinity label or a linker group carrying a reactive group (or latent reactive group) that allows selective attachment of the oligomer or polymer (protein or peptide) to a phosphate label, to an affinity label or to a solid support. The method can be combined with well-known methods of mass spectrometry to detect and identify phosphopeptides and phosphoproteins.

To obtain a composition for caries prevention, which has effects for efficiently suppressing demineralization and promoting remineralization and stability in keeping for a long time, 0.01 to 10% by weight of sodium carboxymethylcellulose having the etherification degree of 0.7 to 1.0 is used in a composition blended with a casein phosphopeptide-amorphous calcium phosphate complex (CPP-ACP) and / or a casein phosphopeptide-amorphous calcium fluoride phosphate complex (CPP-ACFP), a sodium carboxymethylcellulose, a viscosity regulator and water.

The invention provides a fixed titanium ion affinity chromatographic material and the preparation and the application thereof. The strong mutual action between a titanium ion and a phosphate group on a phosphoric acid modified solid phase carrier is used for fixing the titanium ion on the carrier. The fixed titanium ion affinity chromatographic material which is enriched in phosphopeptide is used by the invention. Due to the strong chelating function between the fixed titanium ion and the phosphopeptide, the phosphopeptide is kept on the affinity chromatographic material to be separated from the fixed titanium ion.

The present invention relates to amino acid humanized infant formula milk powder and a preparation method thereof. The amino acid humanized infant formula milk powder comprises the following materials by a certain weight percent: lactose, demineralised whey powder, vegetable oil, skimmed milk powder, hydrolyzed whey protein, galactooligosaccharides, fructo oligosaccharide, a mineral premix, ARA, casein hydrolysate, whey protein concentrate, soybean phospholipids, DHA, a water-soluble vitamin premix, casein phosphopeptides, lactoferrin, L-tyrosine, nucleotide, a lipid soluble vitamin premix, L-tryptophan, L-methionine and taurine. The present invention also includes a method for preparing the amino acid humanized infant formula milk powder. The present invention can effectively solve the problem of pool protein absorption and digestion effect and the low protein utilization rate of infants during infant period.

The invention provides a nutritional composition suitable for patients suffering from bone and joint diseases. The nutritional composition is prepared from 20%-40% of marine fish oligopeptides, 10%-30% of ossein protein peptides, 1%-5% of organic calcium, 1%-5% of casein phosphopeptides, 0.1%-1% of a saussurea involucrate culture, 1%-5% of shark cartilage powder, 5%-20% of inulin, 5%-30% of oligopeptides, 0.1%-1% of vitamins, 1%-3% of mineral substances and the like. The marine food which is used for special dietary uses, is suitable for being eaten by the patients suffering from the bone and joint diseases and contains the marine fish oligopeptides and the ossein protein peptides is provided according to physiological characteristics, nutritional requirements and conventional diet defects of the patients suffering from the bone and joint diseases, and the food has the effects of improving the nutritional condition of the organism, promoting rehabilitation of bones and joints, assisting in controlling blood glucose and conditioning the intestinal functions of the organism and the like.

The present invention provides a method for the diagnosis of tauopathies in an individual and / or for the differential diagnosis of a tauopathy versus a non-tauopathy based on the detection of the ratio of phospho-tau (181) / total tau in said individual. The present invention further provides a phospho-peptide for standardization in a method of the invention.

The invention provides product compositions and a preparation method of neutral liquid high calcium milk. Every 100 weight portions of the neutral liquid high calcium milk comprises the following compositions in portion by weight: 97.50 to 99.05 portions of milk, 0.40 to 0.80 portion of milk calcium, 0.10 to 0.50 portion of hydrolyzed collagen, 0.05 to 0.30 portion of casein phosphopeptides (CPP), 0.20 to 0.60 portion of stabilizing agent and 0.10 to 1.00 portion of dietary fiber. The neutral liquid high calcium milk has high safety and nutrition, good taste, high calcium content and balanced calcium-phosphor proportion, and is easy to be digested and absorbed by human body; moreover, the high calcium milk can meet the requirements of consumers of different age groups on calcium supplementation and is favorable for strengthening bone and articular cartilage.

The invention discloses a compound type biological active peptide product for livestock and poultry, which is formed by mixing the following components in percent by mass: 1%-7% of functional active peptide, 10%-40% of probiotics, 5%-20% of prebiotics, and the balance of auxiliary materials, wherein the functional peptide is one or a combination of several of collagen peptide, glutathione, muramyl dipeptide, soybean protein peptide and casein phosphopeptide. The probiotics refers to one or a combination of several of lactic acid bacteria, saccharomycetes, photosynthetic bacteria, actinomycetes, bacillus and streptococcus faecalis. The prebiotics is one or a combination of several of isomalto-oligosaccharide, fructo-oligosaccharide, galactooligosaccharide and xylo-oligosaccharide. The auxiliary materials refer to one or a combination of several of silkworm chrysalis, fish meal, montmorillonite, diatomite, zeolite powder, corn flour, bran and bean pulp. Additive amount of the product accounts for 0.1%-0.2% of the amount of feed, and the feed can be directly fed after the product is added and uniformly mixed. The compound type biological active peptide product disclosed by the invention belongs to the micro-ecological technical product and is green and environment-friendly.

An antioxidant composition having enhanced oxidative stability, emulsion stability, and health benefits. The composition may include individual ingredients or a synergistic blend of non-reducing sugars, sugar polyols, medium-chain triglycerides, polysaccharides, polyphenols, phospholipids, chitosan, and alpha-casein, beta-casein, kappa-casein or protein fragments, glycopeptides, phosphopeptides. The composition may optionally be further utilized for the prevention of hypercholesterolemia or bone mineral loss.

The invention relates to a method for producing casein phosphopeptide by using milk, which is performed according to the following process steps: 1) defatting and removing impurities: using a butterfly type centrifuge to remove fat and the impurities through secondary centrifugal separation at the temperature of 30-40 DEG C; 2) adding an enzyme for hydrolysis: adding 0.5% trypsinase into the defatted milk, using a 0.3-0.7mol / l sodium hydroxide solution to regulate pH to 7.5-8.5 while adding, hydrolyzing for 1.5-2.5 hours, raising the temperature to 70-90 DEG C and inactivating the enzyme within 30 minutes; 3) debitterizing and purifying: using a stirring machine at the rotational speed of 30-50r / min to stir an enzymatic hydrolysis solution; adding active carbon powder which accounts for 0.05-0.1% of the weight of inventory rating; regulating the pH value to 4.2-5.0; and using a diatomaceous earth filtering machine to filter; and 4) homogenizing and spray-drying: sterilizing the liquid casein phosphopeptide at the temperature of 80-100 DEG C for 15-30 seconds, and then passing through a homogenizer at the homogenizing pressure of 15-25MPa; and further passing through a spray-drying tower for getting the powdery casein phosphopeptide. The content of the phosphopeptide achieves 20%, the purity is high, and the casein phosphopeptide only has light bitterness and is good in color and luster.

The invention relates to a novel calcium supplementing preparation with functions of improving bones and joints, which is prepared from the following raw materials in parts by weight: 10-42 parts of calcium source components, 5-25 parts of amino sugar components, 0.1-5 parts of casein phosphopeptide, 0.5-5 parts of bone collagen protein, 0-8 parts of oxidation resistant components for human bodies and 0-3 parts of filling agents. The novel calcium supplementing preparation not only can supplement a calcium source to increase the bone hardness, but also can supplement bone collagen protein to increase the bone toughness for preventing calcium loss; the casein phosphopeptide is added to promote the calcium absorption, thereby solving the problem of the calcium absorption in the calcium supplementing process; and simultaneously, the oxidation resistant components and the components for promoting osteoblast proliferation are added to enable the bone reconstruction process formed by bone formation and bone absorption to balance again, thus the supplemented calcium can be reserved and firmly deposited on the bones. The related components in the novel calcium supplementing preparation can restore cartilages, improve the joint structure and interdict the deterioration of osteoarthritis.

This invention relates to growth regulatory proteins expressed in various disease states, especially receptor tyrosine kinases and similar growth factor receptors. Prior to the Applicants invention, it was not clear that specific antibodies could be generated that recognized peptide epitopes comprising phosphotyrosine in the context of its surrounding amino acid sequence. Applicants generated antibodies to such phosphotyrosine specific peptides, distinct from antibodies that recognize only phosphotyrosine itself. The invention includes methods for producing phosphopeptide specific antibodies by removing contaminating antibody specificities by negative and / or positive selections. Phosphospecific antibodies and their uses in immunodetection, diagnostic or therapeutic applications are also disclosed.

The present invention describes novel tumor-specific phosphorylated peptides, nucleic acids encoding those peptides, and antibodies generated against said peptides. The genes, peptides, and antibodies described herein may be used as diagnostic indicators of the presence of cancer and / or used in therapeutics to treat cancer.

The invention relates to a separation and purification technique, in particular to an affinity chromatography stationary phase of immobilized metal and a preparation method thereof. The structure of the stationary phase is shown in the drawing; wherein, the particle size of GMAPolymer micro-sphere is 10nm-50um; the GMAPolymer micro-sphere is poly-methacrylic acid glycidyl esters micro-sphere. By carrying out amination and phosphoric esterification, a synthesis method of the high-performance poly-methacrylic acid glycidyl ester-group novel affinity chromatography stationary phase is gained and used for the research in phosphorylation proteomics and highly-selective separation, enrichment and purification of phosphopeptide by the chelation with zirconium ions and iron ions; meanwhile, compared with the traditional stationary phase, the affinity chromatography stationary phase reduces the nonspecific adsorption on the non-phosphopeptide.

The invention relates to a composition with a function of increasing bone density and a preparation method thereof. Active components comprised by the composition are calcium carbonate, glucosamine hydrochloride, chondroitin sulfate, casein phosphopeptide, and soy isoflavone. The composition is prepared by reasonably and scientifically matching and combining the raw materials, and is prepared into oral preparations which are convenient to take. Experiments prove that the composition has the function of increasing the bone density.

The invention discloses a preparation method of high-purity casein phosphopeptide. The method comprises the following steps: adding water to dissolve casein; carrying out secondary enzymolysis by using compound protease and pepsase prepared from any four of trypsase, neutral protease, alkaline protease, curd protease and nematolyt; carrying out secondary purification treatment through cation exchange resin and anion exchange resin after sterilizing and filtering; washing hydrolysate by water; eluting by hydrochloric acid of which the concentration is 0.5-1%; desalting and drying by a nanofiltration membrane. By adopting the preparation method, the yield (greater than or equal to 17%) and the purity (greater than or equal to 90%) of the casein phosphopeptide can be effectively improved; the prepared casein phosphopeptide is bitter in taste, and good in water-solubility; a water solution containing 20% of casting polypropylene (CPP) is clear and transparent; the casein phosphopeptide is powder of which the color is pure white. The molecular weight of the milk casein phosphopeptide prepared by the method mainly is between 1000 and 5000; absorption of a human body is facilitated; the preparation method disclosed by the invention is a preparation method which is low in cost, simple in process, and applicable to industrial production.

The invention discloses a functional nutriment for promoting lactation and recuperation of postpartum women, which is characterized by comprising the following raw materials: marine proteins, papaya, calcium preparations, dried marine algae powder, jujube powder, olive oil, casein phosphopeptides and active probiotics. The functional nutriment is powder, an effervescent tablet or suspension. The invention also discloses a method for preparing the functional nutriment into different forms. The functional nutriment is basically suitable for the postpartum women, has reasonably prepared nutrient components, enhances the effects of the active components, has high quality proteins, is abundant in unsaturated fatty acid, has certain galactagogue effect, is easy to digest and absorb, can improve the immunity of the body and promote recuperation of the body, has certain effect on stabilizing the emotion, is beneficial to relieving postpartum depression and better ensures recuperation and the health of the postpartum women while promoting lactation, and the predigesting and comprehensively balanced nutrition of the functional nutriment is beneficial to organic regulation of the body functions of the postpartum women.

Phosphopeptides containing the Ser(P) cluster sequence motif Ser(P)-Ser(P)-Ser(P)-Glu-Glu- can stabilise their own weight in amorphous calcium phosphate (ACP) [Ca3(PO4)1.87(HPO4)0.2xH2O] and amorphous calcium fluoride phosphate (ACFP) [Ca8(PO4)5FxH2O]. The amorphous phases stabilised by the phosphopeptides are an excellent delivery vehicle to co-localise Ca, F, and phosphate at the tooth surface in a slow-release amorphous form producing superior anticaries efficacy. These amorphous phases stabilised by the phosphopeptides also have utility as dietary supplements to increase calcium bioavailability and to help prevent diseases associated with calcium deficiencies.

Phosphopeptides containing the Ser(P) cluster sequence motif Ser(P)-Ser(P)-Ser(P)-Glu-Glu- can stabilize their own weight in amorphous calcium phosphate (ACP) [Ca3(PO4)1.87(HPO4)0.2xH2O] and amorphous calcium fluoride phosphate (ACFP) [Ca8(PO4)5F x H2O]. The amorphous phases stabilised by the phosphopeptides are an excellent delivery vehicle to co-localise Ca, F, and phosphate at the tooth surface in a slow-release amorphous form producing superior anticaries efficacy. These amorphous phases stabilised by the phosphopeptides also have utility as dietary supplements to increase calcium bioavailability and to help prevent diseases associated with calcium deficiencies.

The invention discloses a health-care food of iron zinc calcium tablets and a production method thereof. The iron zinc calcium tablets consist of chewable tablets, calcium carbonate, ferric pyrophosphate, zinc gluconate, lactose, sucrose, sorbitol, mannitol, maltodextrin, casein phosphopeptide, milk powder, fruit powder, citric acid, sodium citrate, magnesium stearate, silicon dioxide and other raw materials by parts by weight. The prepration method comprises the steps of preparing the raw materials by parts by weight, smashing, screening, weighing the raw materials, granulating, baking, mixing, tabletting and coating. The product has the effects of reinforcing spleen, promoting appetite, improving myocardial ischemia, supplementing blood and calcium, improving sleep and preventing iron deficiency anemia and other diseases, thereby being the health-care product which can not only strengthen the body, but also improve the immunity and the anti-disease ability of the human body.