39 results about "Phosphopeptide" patented technology

The invention discloses a nutritional composition for promoting central nervous system development and application. The invention provides a nutritional composition for promoting the development of a central nervous system. The nutritional composition comprises the following necessary components: a breast milk oligosaccharide component and a casein phosphopeptide component, moreover, in the composition, the mass ratio of the breast milk oligosaccharide component to the casein phosphopeptide component is (1-100): (500-1). According to the invention, the breast milk oligosaccharide component and the casein phosphopeptides are matched to prepare the nutritional composition, and the nutritional composition can significantly promote nerve development such as neuronal maturation, synaptic generation and myelin sheath formation.

The present application relates to a kind of bone repair compositions and its preparation method, belong to biological medicine technical field, pharmaceutical composition includes acid anhydride bovine beta-lactoglobulin peptide, casein phosphopeptide, yak bone peptide, loquat leaf extract, pterostilbene, honeysuckle chlorogenic acid and cyperus rotundus extract;Acid anhydride bovine beta-lactoglobulin peptide is prepared using special enzymolysis method.Acid anhydride bovine beta-lactoglobulin peptide, casein phosphopeptide and yak bone peptide are loaded using carboxymethyl chitosan-hydroxyapatite composite microspheres, then using sodium alginate-sodium carboxymethyl cellulose containing loquat leaf extract, pterostilbene, honeysuckle chlorogenic acid and cyperus rotundus extract one coating, then using sodium alginate-sodium carboxymethyl cellulose secondary coating.The present application develops high-efficiency pharmaceutical combination component, using polymer material to coat drug to form sustained-release system, improve bioavailability, using granular carrier to increase the stability and solubility of drug, promote intestinal absorption, improve bone repair process.

The invention relates to the field of hair growth nutrients, and discloses a nutrient composition for promoting hair growth and a preparation method thereof, the composition comprises a basic hair growth nutrient group and an absorption-promoting synergistic group; the basic hair growth nutrition group comprises the following components in parts by weight: 0.3-0.8 part of ferrous lactate, 2-5 parts of zinc gluconate, 5-8 parts of magnesium stearate, 3-8 parts of a coating material and 17-25 parts of milk mineral salt; the absorption-promoting synergistic group comprises the following components in parts by weight: 0.2-1.5 parts of a black pepper extract, 2-10 parts of lecithin and 2-8 parts of casein phosphopeptides, the black pepper extract and the lecithin are firstly released, an intestinal epithelium channel is opened by utilizing a biological penetration promoting effect, and a pre-permeation state is established; the subsequently released CPP is complexed with divalent metal ions in intestinal juice to prevent precipitation, so that the CPP is synchronously and rapidly absorbed through a pre-opened channel, and the problems of competitive antagonism and difficult absorption of high-dose minerals are effectively solved.

The present invention relates to the field of nutraceutical compositions aimed at enhancing calcium absorption. More particularly, the invention provides a bioactive composition comprising a synergistic combination of casein phosphopeptides and Parmelia perlata, in conjunction with pharmaceutically acceptable excipients. The unique formulation of these components synergistically promotes improved calcium absorption, thereby offering enhanced nutritional and therapeutic benefits.

The invention belongs to the technical field of analytical chemistry, and particularly relates to a preparation method of a directional pore channel integral material and a method for constructing an enrichment device and establishing a rapid phosphopeptide enrichment method by using the directional pore channel integral material. The preparation method comprises the following steps: preparing a CS-PC integral material with directional pore channels from chitosan, zirconium amino phthalocyanine and polyethylene glycol diglycidyl ether through a directional freezing method and a chemical crosslinking method, and then modifying the CS-PC integral material with 5 '-phosphopyridoxal and zirconium oxychloride to obtain the CS-PC PLP integral material. The CS-PC-PLP integral material prepared by the invention has anisotropy and a directional pore channel structure, is convenient to fix in a pipette tip and is combined with a row pipettor for rapid enrichment; the CS-PC coated PLP integral material has the advantages of green and cheap raw materials and strong reusability.

Composition comprising a mineral combination: calcium, magnesium, zinc; an amino acid combination: L-lysine, L-tryptophan or other amino acids; a vitamin combination: vitamin D3, vitamin K2, vitamin A; a bioactive peptide combination: casein phosphopeptide, collagen tripeptides, egg yolk tripeptides / hydrolyzed egg yolk flour.

The application discloses a kind of bifunctional magnetic nano material and its preparation method and application.The nano material described in the application is Fe3O4@ZrO2@TiO2@mSiO2-L-Cys, with spherical structure;The material includes successively from inside to outside: the inner core is the magnetic nanoparticle of ferriferrous oxide;Intermediate layer is zirconium dioxide and titanium dioxide composite metal oxide layer;Sub-outer layer is mesoporous silica layer;The outermost layer is the L-cysteine molecular layer grafted by zirconium-sulfur chemical bond;The preparation method described in the application adopts solvothermal method to synthesize magnetic core, functional layer is coated by hydrothermal reaction, co-condensation method constructs mesoporous shell, and hydrophilic group is introduced by chemical grafting.The bifunctional magnetic mesoporous nano material prepared in the application is applied to the effective enrichment of glycopeptide and phosphopeptide in standard protease solution mixture, and the detection sensitivity of phosphopeptide and glycopeptide reaches 1 fmol / μL, which has wide prospect in glycopeptide and phosphopeptide proteomics analysis research.

The present application belongs to the field of bionic technology, and particularly relates to a kind of bionic material loaded with YD monomer and its preparation method and application.The present application utilizes YD monomer composed of tyrosine (Y) and aspartic acid (D) to synthesize a kind of bionic material through reversible addition-fragmentation chain transfer (RAFT) polymerization. The bionic material can produce spatially adjacent binding pockets through the covalent bond of tyrosine (Y) and aspartic acid (D), and can capture two kinds of PTM (phosphopeptide and O-glycopeptide) simultaneously through synergistic interaction. The results of the present application show that the bionic material of YD monomer optimizes charge balance, the neutral phenolic group of Y (pKa ~ 10) and the negatively charged carboxylate of D (pKa ~ 3.9) together reduce the electrostatic repulsion with phosphopeptide, while maintaining target affinity; self-assembled into nanostructured porous framework can expose multivalent binding sites, and significantly improve capacity and selectivity through synergistic effect.

The present invention relates to improved phosphopeptide stabilised amorphous calcium phosphate and / or amorphous calcium fluoride phosphate complexes and compositions containing those complexes. Methods of making the complexes of the invention and of treating or preventing oral conditions are also provided. In one aspect, the present invention provides a composition comprising equal to or greater than about 8% w / v stannous associated phosphopeptide (PP) stabilized amorphous calcium phosphate (ACP) or amorphous calcium fluoride phosphate (ACFP) complexes wherein the complexes have a stannous ion content of equal to, or greater than, 1 mole of stannous per mole of PP but less than 4 moles of stannous per mole of PP.

The present invention relates to improved complexes of amorphous calcium phosphate and / or amorphous calcium fluoride phosphate stabilised by phosphopeptides / phosphoproteins by addition of stannous ions. These complexes have anticariogenic properties useful to protect tooth structures as they remineralize (repair) early stages of dental caries and have other dental / medical applications (including anti-calculus, anti-erosion / corrosion and anti-dentinal hypersensitivity). Methods of making the complexes of the invention and of treatment or prevention of various dental conditions including dental caries, dental calculus, dental erosion / corrosion and dental hypersensitivity are also provided.

The application discloses a kind of magnetic material for synchronously enriching glycopeptide and phosphopeptide and its preparation method and application.The nanomaterial Fe3O4@nSiO2@TiO2@mSiO2-L-Cys described in the application;The material includes successively from inside to outside: the magnetic core of ferriferrous oxide;Non mesoporous silica intermediate layer coated on the surface of the core;Titanium dioxide functional layer coated on the surface of the intermediate layer;And the outermost layer is the ordered radial dendritic mesoporous silica shell layer modified by L-cysteine;The preparation method described in the application adopts solvothermal method to synthesize magnetic core, functional layer is coated by hydrothermal reaction, co-condensation method is used to build mesoporous shell layer, and hydrophilic group is introduced by chemical grafting.The prepared magnetic material of the application is applied to the effective enrichment of glycopeptide and phosphopeptide in standard protease hydrolysis mixed liquor, and the detection sensitivity of phosphopeptide and glycopeptide reaches 0.1fmol / µL and 1fmol / µL respectively, which has wide prospect in glycopeptide and phosphopeptide proteomics analysis research.

The invention relates to application of casein phosphopeptides in preparation of products for promoting neurodevelopment. The casein phosphopeptides can promote neuronal differentiation and synaptic formation by regulating secretion of neurotransmitters and regulating expression of neuronal differentiation related genes.

Provided are TCRs (e.g., TCRs that bind to MLL, e.g., TCRs that bind to an MLL phosphopeptide, e.g., TCRs that bind to an MLL phosphopeptide / MHC complex), cells and pharmaceutical compositions comprising these TCRs, nucleic acids encoding these TCRs, expression vectors and host cells for making these TCRs, and methods of treating a subject using these TCRs.

The invention discloses a preparation method of large yellow croaker egg protein iron ion chelated phosphopeptides. The preparation method comprises the following steps: preparing a large yellow croaker egg protein solution, adjusting pH and heating, performing specific enzymolysis and centrifugation, and freeze-drying to obtain phosphopeptides. The phosphopeptide component is directly prepared from the large yellow croaker egg protein by utilizing a one-step enzymolysis method, the hydrolysis degree of the prepared large yellow croaker egg protein phosphopeptide is 40.79-42.25%, the N / P molar ratio is 0.35-0.40, and the IC50 value is 0.04-0.06 mg / mL, the steps are simple, the operation is convenient, the phosphopeptide is effectively extracted from the large yellow croaker egg, and the preparation method has the advantages that the cost is low, and the application prospect is wide. The prepared phosphopeptide has the ferrous ion chelation capability which is obviously higher than that of casein phosphopeptide, and can be applied to the food industry as a novel nutritional supplement.

The invention discloses a composition containing gamma-aminobutyric acid for promoting growth and development of children and a preparation method thereof, the composition comprises gamma-aminobutyric acid and two newly designed modified compounds N-(4-trifluoromethylbenzyl)-gamma-aminobutyric acid amide and a gamma-aminobutyric acid-zinc complex, and functional components such as animal bifidobacterium subsp.lactis, hydrolyzed yolk powder, casein phosphopeptides, minerals and theanine are supplemented. The preparation process comprises the following steps: respectively dissolving and homogenizing the components, mixing step by step in a protective atmosphere, and finally degassing, filling and performing low-temperature irradiation sterilization. The two modified compounds are prepared through activation amidation and complex reaction respectively, and the blood-brain barrier transmittance, stability and bioavailability of original components are remarkably improved. The composition can effectively promote secretion of growth hormones, improve bone growth and improve sleep quality through a multi-target synergistic effect, and is suitable for promoting growth and development of children.

This invention relates to the field of infant formula technology, and discloses an infant formula milk powder and its preparation method. The infant formula milk powder is obtained by mixing demineralized whey powder, whey protein powder, fructooligosaccharides, choline chloride, taurine, fat molecule inclusion complex, complex mineral casein phosphopeptide chelate, water-soluble vitamin premix, and fat-soluble vitamin premix. The complex mineral casein phosphopeptide chelate is prepared from casein phosphopeptide, deionized water, sodium hydroxide aqueous solution, vitamin C, calcium citrate, ferrous sulfate, and zinc sulfate. The fat molecule inclusion complex is prepared from β-cyclodextrin, deionized water, walnut oil, docosahexaenoic acid, and anhydrous ethanol. This invention can delay the oxidation of unsaturated fatty acids in infant formula.

The invention relates to the technical field of probiotic preparations, and particularly discloses a multifunctional probiotic preparation and a preparation method thereof. According to the invention, bifidobacterium animalis Bb-12, lactobacillus rhamnosus GG and phytobacterium plantarum LP are compounded, the three strains have a synergistic effect to realize comprehensive intestinal regulation, modified chitosan is adopted as a coating, a compact structure of a covalent skeleton formed by cross-linking genipin, amino acid and casein phosphopeptides are complexed step by step, gastric juice erosion can be resisted, and the intestinal tract can be fully regulated. Thalli are prevented from being damaged by gastric acid; the amino acid in the coating layer improves the intestinal mucosa adhesion ability of the probiotics, and the casein phosphopeptides promote mineral absorption and cooperate with the probiotics to further strengthen the intestinal regulation effect.

The application provides a casein phosphopeptide with the functions of promoting the absorption of calcium, iron, zinc, manganese and magnesium and improving bone health and a preparation method, and belongs to the technical field of casein phosphopeptide and comprises the following steps: S1, taking casein and adding pepsin for first enzymolysis; S2, taking the first enzymolysis solution, adjusting pH and temperature, and adding a compound enzyme for second enzymolysis; S3, after the second enzymolysis solution is inactivated, the casein phosphopeptide is obtained through treatment. The casein phosphopeptide prepared by the application has the following advantages: 1. can effectively complex mineral elements and reduce ion channel competition inhibition; 2. promotes the absorption of calcium, increases the hardness of the skeleton, and promotes the growth and development of the skeleton; 3. promotes the absorption of magnesium, assists calcium deposition, reduces bone loss, prevents osteoporosis, 3. promotes the absorption of iron, prevents iron deficiency anemia, and improves uncomfortable symptoms such as dizziness and fatigue; 4. promotes the absorption of zinc, enhances the activity of immune cells, improves resistance, and improves appetite. 5. promotes the absorption of manganese, activates the development of the skeleton, accelerates energy metabolism, and has antioxidant effect.

The present invention is related to a bioactive peptide composition More particularly, the present invention is particularly related to a bioactive peptide composition comprising Casein phosphopeptides, Isoleucyl-prolyl-proline and Valyl-prolyl-proline and one or more inactive excipients and process for preparing the same. The invention intended to enhance calcium absorption or improve calcium incorporation into bones.

The application discloses an eggshell membrane peptide for enhancing bone density and a preparation method and application thereof, belongs to the technical field of egg by-product processing, and particularly relates to a preparation method of an eggshell membrane peptide for enhancing bone density, which comprises the following steps: vacuum freeze-drying eggshell membranes, crushing the eggshell membranes to obtain eggshell membrane powder; mixing the eggshell membrane powder with deionized water to obtain an eggshell membrane solution; adding keratinase, performing enzymolysis, and taking supernatant; adding collagenase, performing enzymolysis, centrifuging to take supernatant, and obtaining a primary enzymolysis liquid; adding alkaline phosphatase, performing enzymolysis, centrifuging to take supernatant, and obtaining an enzymolysis liquid; performing ultrafiltration, and drying to obtain the eggshell membrane peptide. The application utilizes keratinase to break the cross-linking of keratin by enzymolysis, utilizes collagenase to release active peptides capable of stimulating the proliferation of osteoblasts by enzymolysis, further utilizes alkaline phosphatase to activate phosphopeptide calcium binding sites by enzymolysis, promotes the deposition of calcium salt, and provides the eggshell membrane peptide for enhancing bone density.

The application provides a casein phosphopeptide and a preparation method thereof, and belongs to the technical field of active peptides. The application takes cow milk casein as raw material, separates calcium-sensitive protein through low-temperature continuous phase transformation, redissolves the calcium-sensitive protein to obtain supernatant, dries the supernatant to obtain enriched beta-casein, and prepares the enriched beta-casein into casein phosphopeptide through enzymolysis. The application takes casein with a market price lower than 200 yuan / kg as raw material, and the simple preparation method reduces the dosage of low-cost reagents, so that the preparation cost of the casein phosphopeptide is greatly reduced. Moreover, compared with CPPs, the beta-CPPs prepared by the application further reduce the preparation cost of the CPPs. In addition, the preparation method provided by the application has strong controllability and simple operation, can realize efficient and stable production of the CPPs, and is suitable for industrial scale preparation.

The invention provides a compound composition for cultivating primordial qi and promoting growth and a preparation process thereof, and the preparation process comprises the following steps: adding water into traditional Chinese medicine components, carrying out high-temperature extraction to obtain a basic water extract, and filtering and concentrating the basic water extract to obtain a concentrated water extract; adding a compound enzyme into the concentrated water extract for enzymolysis to obtain an enzymatic hydrolysate, adding diatomite into the enzymatic hydrolysate for targeted adsorption and filtration to obtain a primary filtrate, cooling the primary filtrate to room temperature, adding acid to adjust the pH value to 5.8-6.2, and adding a xanthan gum water solution to obtain a traditional Chinese medicine water extract; adding a bacterial suspension into the traditional Chinese medicine water extract to obtain a traditional Chinese medicine compound liquid, wherein the bacterial suspension contains inactivated lactobacillus helveticus CCFM1263; mixing bone collagen peptide and casein phosphopeptide to obtain a mixture, and dissolving the mixture in purified water in which sodium citrate is dissolved in advance to obtain a peptide premixed solution; slowly adding the peptide premixed liquid into the traditional Chinese medicine composite liquid, fully mixing, filtering and sterilizing to obtain the compound composition for cultivating the primordial qi and promoting growth.

The present invention relates to nutritional compositions, products comprising them and uses thereof. The nutritional composition comprises casein phosphopeptides and choline chloride, wherein the ratio of the mass of the casein phosphopeptides to the total mass of choline converted from choline chloride is 1: 0.2 to 1: 8.0. The nutritional composition can synergistically regulate the innate immunity of the body, for example, improve the expression of genes related to the innate immunity of the body.

The invention discloses a bifidobacterium animalis subsp. Lactis BD108 preparation as well as application and a product of the bifidobacterium animalis subsp. Lactis BD108 preparation in emotion regulation, and belongs to the technical field of microorganisms. The invention aims to solve the technical problems that the survival rate of viable bacteria is low after oral administration, the mood improvement effect is unstable and long-acting intervention is difficult to realize due to the problems of weak strain specificity, insufficient mood regulation activity, poor preparation protection effect, single action pathway and the like existing in the conventional probiotic strain in mood regulation application. According to the key point of the technical scheme, the freeze-dried microsphere preparation of the animal bifidobacterium subsp. Lactis BD108 is provided and comprises the animal bifidobacterium subsp. Lactis BD108 and a composite carrier solution, the preservation number of the animal bifidobacterium subsp. Lactis BD108 is GDMCC (China General Microbiological Culture Collection Center) No.67826; the composite carrier solution is composed of trehalose, pullulan, whey protein phosphopeptides and a solvent.

The invention belongs to the technical field of food, and relates to an application of casein phosphopeptides in regulating tryptophan metabolism of an organism. Research results show that the casein phosphopeptides are obtained through a serotonin metabolic pathway and / or a kynurenine metabolic pathway; the content of tryptophan in a body is increased, and / or the content of at least one metabolite (5-methoxyindole-3-acetic acid, N-acetyl serotonin, kynurenine, 2-picolinic acid and indole-3-formaldehyde) in a tryptophan metabolic pathway is increased, so that the effects of emotion regulation, sleep regulation and / or neuroprotection are favorably exerted. Besides, the invention has no special requirements on the specific application mode of the casein phosphopeptides and the form of food containing the casein phosphopeptides, has convenience in implementation, and also provides a new thought for the application research of the casein phosphopeptides.

The invention belongs to the technical field of proteomics, and particularly relates to a phosphorylated peptide fragment enrichment identification method for trace tissues. The method comprises the following steps: (1) adding a lysis solution into a trace tissue sample, and heating; performing ultrasonic treatment on the sample in an ice-water bath; adding enzyme for enzymolysis; adding a solution containing a phosphopeptide enrichment material into the sample for incubation; activating and balancing a peptide fragment desalting column by using two enrichment buffer solutions; (2) adding a sample and a mixed solution containing the phosphopeptide enrichment material into the pretreated desalting column; cleaning with a cleaning buffer solution A and a cleaning buffer solution in sequence; eluting with two elution buffer solutions in sequence; and collecting the eluent, and carrying out vacuum concentration and freeze-drying to obtain the enriched phosphorylated peptide fragment of the trace tissue sample. The method can reduce treatment steps and reduce liquid transfer, so that sample loss can be reduced; the pretreatment time is greatly saved; the identification of the phosphorylation site of the protein with the initial quantity as low as 10 ng is realized.

The invention belongs to the technical field of biology, and particularly relates to a gamma-aminobutyric acid composition for promoting growth of children and a preparation method of the gamma-aminobutyric acid composition. The gamma-aminobutyric acid composition for promoting the growth of the children is prepared from the following specific components in parts by weight: 1 to 10 parts of gamma-aminobutyric acid, 0.01 to 10 parts of L-lysine, 0.01 to 15 parts of hydrolyzed yolk powder, 0.01 to 5 parts of casein phosphopeptides, 0.01 to 3 parts of bovine spleen peptides, 0.01 to 8 parts of dietary fibers and 10 to 100 parts of sweetening agents. According to the present invention, the low-temperature or short-time high-temperature treatment is adopted through the raw material drying at the temperature of 60-80 DEG C and the liquid beverage sterilization process, such that the activity of the heat-sensitive nutritional components such as GABA, L-lysine, peptides and the like is prevented from being damaged by the high temperature, the core effect of the finished product on promoting the growth of children is ensured, and the sealed packaging of the finished product can isolate the air, the moisture and the pollution so as to provide the health-care product. Oxidation loss of nutritional ingredients is reduced, the shelf life of the product is prolonged, and meanwhile, sanitation and safety of the product in the storage process are guaranteed.

The present invention relates to improved complexes of amorphous calcium phosphate and / or amorphous calcium fluoride phosphate stabilised by phosphopeptides / phosphoproteins by addition of stannous ions. These complexes have anticariogenic properties useful to protect tooth structures as they remineralize (repair) early stages of dental caries and have other dental / medical applications (including anti-calculus, anti-erosion / corrosion and anti-dentinal hypersensitivity). Methods of making the complexes of the invention and of treatment or prevention of various dental conditions including dental caries, dental calculus, dental erosion / corrosion and dental hypersensitivity are also provided.

This invention provides a glycosylated peptide chelated iron pyrophosphate supplement, its preparation method, and its application. The raw materials for preparing the glycosylated peptide chelated iron pyrophosphate supplement include glycosylated peptides, iron pyrophosphate, and a solubilizer. The glycosylated peptide is a β-glucan-grafted casein phosphopeptide, and the solubilizer is sodium pyrophosphate. The glycosylated peptide chelated iron pyrophosphate supplement of this invention exhibits good water solubility, color stability over a wide pH range, coexistence with nutrients such as vitamin C and oils, high iron loading capacity, good stability in gastric juice, sustained iron release in simulated intestinal fluid, and high iron absorption and utilization rate, significantly improving anemia in iron-deficiency anemia mice. The excellent absorption performance and superior physicochemical properties of the glycosylated peptide chelated iron pyrophosphate supplement of this invention make it a potential candidate for widespread application in the iron supplement market.

The invention belongs to the technical field of food, and relates to novel application of casein phosphopeptides, in particular to application of the casein phosphopeptides in preparation of food beneficial to regulation of dopaminergic neurons of organisms, and regulation of the dopaminergic neurons of the organisms comprises enhancement of the strength of the dopaminergic neurons and / or enhancement of the strength of the dopaminergic neurons. Neurotransmitters and metabolites beneficial to operation of a dopamine energy system are regulated and controlled.