120 results about "Phosphopeptide" patented technology

A synergistic composition for improving the bioaccessibility of minerals comprises lutein, a casein phosphopeptide, or a 1, 3-dioleoyl-2-palmitic acid triglyceride, preferably in combination with one or more non-digestible oligosaccharides. These compositions are useful for the prevention and / or treatment of mineral deficiency and related conditions, preferably anemia, said minerals comprising iron and / or calcium.

The invention provides universal functional formula milk powder for cats and dogs and a preparation method thereof, and relates to the field of pet formula food. The formula milk powder consists of goat milk powder, colostrum powder, whey protein powder, lactoferrin powder, yolk powder, immune globulin powder, compound grease, compound minerals, compound vitamins, choline, taurine, compound prebiotics, compound probiotics, docosahexaenoic acid, arachidonic acid, casein phosphopeptides, L-glutamine and beta-galactosidase. And the goat milk powder, colostrum powder, whey protein powder, lactoferrin powder, yolk powder and immune globulin powder are subjected to low-temperature sterilization treatment by adopting an induced electric field or high-pressure microjet. The cat and dog formula milk powder overcomes the defects in the prior art, through compounding of basic nutritional ingredients and functional ingredients, a novel low-temperature sterilization technology is adopted for sterilization treatment, on the premise that the functionality of the formula milk powder is guaranteed, the nutritional value of the formula milk powder is reserved to a greater extent, and healthy growth and development of cats and dogs are promoted.

The invention discloses a compound preparation of calcium citrate casein phosphopeptide calcium tablets and a preparation method of the compound preparation. The compound preparation comprises the following components in percentage by weight: 90-96% of calcium citrate particles, 0.68-1.84% of casein phosphopeptide, 0.08-0.16% of vitamin D3 powder, 0.7-1.5% of vitamin K2 powder, 0.5-1.5% of sodium carboxymethyl starch and 0.6-1.0% of magnesium stearate. And 2-4% of a coating premix. The preparation method comprises the following steps: step 1, calcium citrate wet granulation; step 2, mixing; step 3, tabletting; step 4, coating; the feed is reasonable in matching and balanced in proportioning, and can supplement calcium, vitamin D and vitamin K. The compound preparation of the calcium citrate casein phosphopeptide calcium tablet can effectively avoid gastrointestinal discomfort and prevent constipation while improving the effective rate of calcium supplement. In addition, the compound preparation of the calcium citrate casein phosphopeptide calcium tablet is good in taste, convenient to carry, easy in raw material obtaining and low in production cost.

The invention belongs to the technical field of biology, and particularly relates to casein phosphopeptides for promoting absorption of calcium, iron and zinc minerals and improving bone health. Casein phosphopeptides containing high-activity peptide fragments are obtained through an optimization process, six mineral elements of calcium, magnesium, copper, iron, zinc and manganese serve as research objects, firstly, mineral utilization of CPP is evaluated through a mineral deficiency rat model, the content of Ca, Mg, Cu, Fe, Zn and Mn in thighbones is increased by 7.12%, 3.56%, 20.76%, 15.84%, 9.94% and 67.12% respectively when the CPP is eaten by 7mg / kg.d; secondly, screening a peptide fragment with calcium absorption promoting activity from the CPP by utilizing a molecular docking technology, and performing functional verification; the invention aims to reveal the action effect of CPP in mineral absorption and utilization from cell and animal levels, and provides a theoretical basis for functional development of CPP.

The invention relates to the technical field of bioanalytical chemistry, in particular to a method for simultaneously enriching and separating glycopeptides and phosphopeptides by using a bionic hydrophilic adsorbent, which specifically comprises the following steps: step 1, preparing a polyether-ether-ketone (PEEK) framework by using a fused deposition modeling method (FDM); 2, soaking the obtained PEEK framework in an aqueous solution containing dopamine hydrochloride and tris (hydroxymethyl) aminomethane, and carrying out a reaction; step 3, then, adding a mixed solution containing hyaluronic acid methacrylate, vinyl phosphoric acid, N, N-dimethyl bisacrylamide and phenyl-2, 4, 6-trimethylbenzoyl lithium phosphate into the PEEK framework; in the invention, in the aspect of material preparation, a fused deposition modeling method (FDM) is adopted to prepare a polyether-ether-ketone (PEEK) frame, and the material has good mechanical properties and chemical stability and can bear a series of subsequent complex treatment processes without being damaged.

The invention discloses sheep blood phosphopeptide chelated iron prepared by a one-pot method, a preparation method and application, and relates to the technical field of preparation methods of peptide chelated iron, the method comprises the following steps: dissolving sheep blood powder in water, and preheating to 50-55 DEG C to obtain a sheep blood powder solution; papain is added into the sheep blood powder solution for enzymolysis, a sheep blood peptide solution is obtained, sodium pyrophosphate is added into the sheep blood peptide solution for phosphorylation modification, and a sheep blood phosphopeptide reaction solution is obtained; adjusting the pH value of the sheep blood phosphopeptide reaction liquid to 3.0-4.0 to dissociate heme groups to obtain dissociation liquid; and centrifuging the dissociation liquid, and recovering the supernatant to obtain the sheep blood phosphopeptide chelated iron. In the preparation process, endogenous iron ions contained in the heme are utilized, so that the complex preparation process of the current blood peptide iron chelate is simplified.

The present invention relates to improved phosphopeptide stabilised amorphous calcium phosphate and / or amorphous calcium fluoride phosphate complexes and compositions containing those complexes. Methods of making the complexes of the invention and of treatment dental hypersensitivity are also provided. In one embodiment, the invention provides a stannous-associated phosphopeptide (PP) stabilized amorphous calcium phosphate (ACP) or amorphous calcium fluoride phosphate (ACFP) complex having a stannous ion content of equal to, or greater than, 1 mole of stannous per mole of PP but less than 4 moles of stannous per mole of PP.

This invention belongs to the field of nutritional composition technology, specifically disclosing a composition for promoting brain cognitive development, its preparation method, and its application. The composition for promoting brain cognitive development provided by this invention includes transforming growth factor, casein phosphopeptide, immunoglobulin, and sn-2 DHA, and further specifies that the mass ratio of transforming growth factor, casein phosphopeptide, immunoglobulin, and sn-2 DHA is (0.1~1.4) ng:(40000~160000) mg:(20000~60000) mg:(20000~70000) mg. In this invention, the preferred ratio of transforming growth factor, casein phosphopeptide, immunoglobulin, and sn-2 DHA can achieve the effect of promoting brain cognitive development through a multi-dimensional synergistic mechanism.

The present invention relates to improved complexes of amorphous calcium phosphate and / or calcium by amorphous fluoride phosphate stabilised phosphopeptides / phosphoproteins by addition of stannous ions. These complexes have anticariogenic properties useful to protect tooth structures as they remineralize (repair) early stages of dental caries and have other dental / medical applications (including anti-calculus, anti-erosion / corrosion and anti-dentinal hypersensitivity). Methods of making the complexes of the invention and of treatment or prevention of various dental conditions including dental caries, dental calculus, dental erosion / corrosion and dental hypersensitivity are also provided.

A peptide composition for improving tear stains of pets belongs to the field of pet health, is used for improving tear stains of pets, and is characterized by comprising 20-30 parts of sea cucumber peptide, 5-10 parts of casein phosphopeptides, 10-20 parts of soybean peptide, 20-30 parts of corn oligopeptide, 10-20 parts of wheat oligopeptide and 10-20 parts of mussel peptide, the amino acid sequence of common mussel peptide is His-Gly-Ala-Asp-Gly-Val-Pro-Pro-Phe, and the amino acid sequence of the common mussel peptide is His-Gly-Ala-Asp-Gly-Val-Pro-Pro-Phe. The amino acid sequence of the perna viridis peptide is Asp-Ser-Ser-Leu-Pro-Pro-Ser-Arg-Pro-Thr, and the perna viridis peptide has the effects of effectively improving the metabolism of ferriporphyrin, inhibiting the activity of saccharomycetes and reducing the formation of tear stains of pets.

The invention provides a combined lactobacillus salivarius YS-UR90 and a preparation method of the combined lactobacillus salivarius YS-UR90. The saliva combined lactobacillus provided by the invention can secrete protease and glycosidase, and the HCT and FLS gene expression levels of the saliva combined lactobacillus are high, so that the saliva combined lactobacillus can promote phenolic acid active ingredients to generate biotransformation during fermentation, and the proportion of chlorogenic acid and the like in fermentation liquor is increased. For example, marine fish oligopeptide and casein phosphopeptides are used as a composite nitrogen source, dandelion powder is used as a functional substrate, and a composite fermentation product obtained through fermentation of saliva and lactobacillus has the effect of promoting uric acid excretion and has a certain protection effect on kidney injury. The compound fermentation product has the effect of improving hyperuricemia mainly by inhibiting XOD activity, increasing serum urea nitrogen level, improving defect expression of uric acid synthesis gene HPRT1, inhibiting expression of uric acid reabsorption URAT1 and GLUT9 genes and improving expression of excretion genes OAT1 and ABCG2.

The invention discloses an eggshell membrane peptide for enhancing bone mineral density as well as a preparation method and application thereof, belongs to the technical field of egg by-product processing, and particularly relates to a preparation method of the eggshell membrane peptide for enhancing bone mineral density, which comprises the following steps: carrying out vacuum freeze drying and crushing on an eggshell membrane to obtain eggshell membrane powder; mixing the eggshell membrane powder with deionized water to obtain an eggshell membrane solution; adding keratinase, performing enzymolysis, and taking supernate; adding collagenase, carrying out enzymolysis, and centrifuging to take supernate, so as to obtain primary enzymatic hydrolysate; adding alkaline phosphatase, performing enzymolysis and centrifugation, and taking supernate to obtain enzymatic hydrolysate; and performing ultrafiltration and drying to obtain the eggshell membrane peptide. According to the eggshell membrane peptide for enhancing the bone mineral density, keratin cross-linking is destroyed through enzymolysis of keratinase, active peptide capable of stimulating osteoblast proliferation is released through enzymolysis of collagenase, phosphopeptide calcium binding sites are further activated through enzymolysis of alkaline phosphatase, calcium salt deposition is promoted, and the eggshell membrane peptide for enhancing the bone mineral density is provided.

The invention discloses a freeze-dried human rabies vaccine and a preparation method thereof, and belongs to the technical field of viral vaccines. The method comprises the following steps: (1) cell culture; (2) virus inoculation; (3) harvesting viruses; (4) concentration and purification: clarifying the harvested virus liquid through a filter, carrying out ultrafiltration concentration through an ultrafiltration membrane to obtain a concentrated solution, and purifying by adopting a column chromatography to obtain a chromatographic solution; (5) preparing a stock solution: inactivating and hydrolyzing the chromatographic solution to obtain a virus inactivation solution, and adding human serum albumin into the virus inactivation solution to obtain the stock solution; (6) preparing a semi-finished product: diluting with a stabilizer to prepare the semi-finished product, wherein the stabilizer comprises the following raw materials: human serum albumin, araboxylan, L-arginine hydrochloride, casein phosphopeptides, alpha-tocopherol nicotinate, a solvent and the like; and (7) preparing a finished product. The rabies vaccine disclosed by the invention is excellent in quality and stable in immune efficacy, and liquid is clear after redissolution.

The invention discloses a nutritional composition for promoting central nervous system development and application. The invention provides a nutritional composition for promoting the development of a central nervous system. The nutritional composition comprises the following necessary components: a breast milk oligosaccharide component and a casein phosphopeptide component, moreover, in the composition, the mass ratio of the breast milk oligosaccharide component to the casein phosphopeptide component is (1-100): (500-1). According to the invention, the breast milk oligosaccharide component and the casein phosphopeptides are matched to prepare the nutritional composition, and the nutritional composition can significantly promote nerve development such as neuronal maturation, synaptic generation and myelin sheath formation.

The present invention relates to improved complexes of amorphous calcium phosphate and / or amorphous calcium fluoride phosphate stabilised by phosphopeptides / phosphoproteins by addition of stannous ions. These complexes have anticariogenic properties useful to protect tooth structures as they remineralize (repair) early stages of dental caries and have other dental / medical applications (including anti-calculus, anti-erosion / corrosion and anti-dentinal hypersensitivity). Methods of making the complexes of the invention and of treatment or prevention of various dental conditions including dental caries, dental calculus, dental erosion / corrosion and dental hypersensitivity are also provided.

The invention discloses a casein peptide composition containing multifunctional peptide and a preparation method of the casein peptide composition. The present invention provides a casein peptide composition containing a multifunctional peptide, characterized in that the casein peptide composition is obtained by enzymatic hydrolysis of a casein liquid, in the casein peptide composition, the relative strength of total functional peptides is 25% or more, the relative strength of casein phosphopeptides is 9% or more, and the relative strength of the casein phosphopeptides is 9% or more. The relative strength of the functional peptides except the casein phosphopeptides is above 11%, and the enzyme in the enzymolysis at least comprises serine protease.

The invention relates to the field of nutritional health food, and discloses a casein modified active peptide for promoting bone repair and a preparation method thereof.The structure of the active peptide sequentially comprises a bone targeting sequence with high affinity to hydroxyapatite, a casein phosphopeptide core sequence capable of chelating calcium ions, a casein phosphopeptide core sequence capable of chelating calcium ions, a casein phosphopeptide core sequence capable of chelating calcium ions, a casein phosphopeptide core sequence capable of chelating calcium ions and a casein phosphopeptide core sequence capable of chelating calcium ions from the C-terminal to the N-terminal. The digestion sequence can be specifically hydrolyzed by matrix metalloproteinase, and the alkynyl functional group is used for covalent cross-linking. The active peptide can be mixed with an azido-containing multi-arm cross-linking agent, and hydrogel can be rapidly formed under physiological conditions through cycloaddition reaction. The system can be gelated in situ and anchored to a bone defect part in a targeted manner, and is triggered by a specific enzyme in a pathological microenvironment to controllably release active peptide fragments capable of chelating calcium ions, so that the concentration of local mineral ions is adjusted, the cell living environment is improved, and an effective new way is provided for promoting bone repair.

A use of a nutritional composition in promoting the bidirectional development of the gut and brain via the gut-brain axis. The nutritional composition comprises the following essential components: docosahexaenoic acid, eicosatetraenoic acid, galactooligosaccharide, 1,3-dioleic acid-2-palmitic acid triglyceride, lutein, nucleotide, lactoferrin, casein phosphopeptide, and probiotics. By combining and strengthening multiple functional active components, the gut health and brain cognitive development of the organism can be bidirectionally promoted by means of the microbiota-gut-brain axis. In particular, the present application can promote the regulation of flora in the gut of the organism, increase the abundance of beneficial bacteria, and decrease the abundance of harmful bacteria related to brain development diseases. The content of butyric acid in the gut can be increased, and the contents of acetic acid and propionic acid are decreased to promote brain and cognitive development.

According to the preparation method and application of the single-cell probiotic coating disclosed by the invention, the coating is used for encapsulating lactobacillus plantarum YJ7 layer by layer by using a metal-phenol network and a chitosan-casein phosphopeptide compound, so that LP-coated TP / Fe < 3 + >-coated CPC is successfully constructed. The functional coating can effectively resist invasion of severe physiological environments of gastrointestinal tracts in vivo and in vitro under the condition that normal activity of LP is not affected, and the survival rate of the functional coating in intestinal tracts is greatly improved. A mouse in-vivo safety research shows that the hydrogel has excellent biocompatibility and harmlessness. In addition, gavage experiments on mice with type 2 diabetes prove that the delivery system enhances the hypoglycemic activity of the lactobacillus plantarum YJ7. In conclusion, the oral probiotic delivery system provides a simple and efficient strategy for enhancing the survival of the probiotics in the severe intestinal environment, and is expected to provide a new view angle for beneficial effects of the probiotics in the intestinal tract.

Methods for inducing anti-phosphorylated Tau antibodies without inducing a serious adverse event in humans are described. The methods include administering to the subject an effective amount of a composition containing a Tau phosphopeptide conjugated to an immunogenic carrier.

The invention relates to the technical field of dairy product processing, in particular to a double-fat double-protein synergistic dairy product and a preparation method thereof. According to the preparation method, a ''whey protein (WP)-casein phosphopeptide (CPP)'' double-protein system and a ''milk fat (MF)-medium chain triglyceride (MCT)'' double-fat system are constructed, and a four-step synergistic process of ''pretreatment, composite emulsification, gradient assembly and low-temperature curing'' is adopted, so that stable combination of a chemical interface of protein and fat is realized, high-temperature stability breakthrough is realized, and the preparation method has the advantages of high-temperature stability, high-temperature stability and high-temperature stability. Meanwhile, high-pressure processing tolerance is improved, fat globule flocculation caused by high pressure is avoided, and long-term storage stability, texture and taste are guaranteed.

The present application relates to a kind of bone repair compositions and its preparation method, belong to biological medicine technical field, pharmaceutical composition includes acid anhydride bovine beta-lactoglobulin peptide, casein phosphopeptide, yak bone peptide, loquat leaf extract, pterostilbene, honeysuckle chlorogenic acid and cyperus rotundus extract;Acid anhydride bovine beta-lactoglobulin peptide is prepared using special enzymolysis method.Acid anhydride bovine beta-lactoglobulin peptide, casein phosphopeptide and yak bone peptide are loaded using carboxymethyl chitosan-hydroxyapatite composite microspheres, then using sodium alginate-sodium carboxymethyl cellulose containing loquat leaf extract, pterostilbene, honeysuckle chlorogenic acid and cyperus rotundus extract one coating, then using sodium alginate-sodium carboxymethyl cellulose secondary coating.The present application develops high-efficiency pharmaceutical combination component, using polymer material to coat drug to form sustained-release system, improve bioavailability, using granular carrier to increase the stability and solubility of drug, promote intestinal absorption, improve bone repair process.

The invention discloses a hydrophilic two-dimensional nano composite material and a preparation method thereof. The material is used for enriching phosphorylated and glycosylated peptides at the same time. The hydrophilic two-dimensional nano composite material is MOLs (at) PEI (at) Au (at) L-Cys and is a lamellar material synthesized by taking a metal organic lamellar material (MOLs) as a substrate material and polyethyleneimine (PEI) as a reducing agent and a stabilizing agent, loading gold nanoparticles (AuNPs) on the surface of the MOLs and then fixing L-cysteine through an Au-S bond. The material has specific hydrophilic interaction with a hydroxyl group on a glucoside chain and a phosphate group of phosphopeptide, so that enrichment of phosphorylated and glycosylated peptides, especially N-phosphorylated peptides, is realized under a neutral condition (pH = 7-7.5). A high-resolution and high-sensitivity liquid chromatography-mass spectrometry system is combined, so that qualitative and quantitative analysis of deep-coverage proteome is realized.

This invention relates to a method for preparing a β-cyclodextrin functionalized covalent organic framework composite material. The method involves an aldehyde-imine condensation reaction between the aldehyde groups on 2,3,4-trihydroxybenzaldehyde and p-phthalaldehyde and the amino group on hepta(6-amino-6-deoxy)betacyclodextrin to construct a β-CD-COF layer. This one-step method produces a β-cyclodextrin covalent organic framework composite material that exhibits excellent enrichment capacity for phosphopeptides, facilitating purification and extraction. It also shows promising application prospects in mass spectrometry detection and exosome separation.

The invention discloses a preparation method of a high-absorptivity calcium supplement of vitamin D3 (VD3) and casein phosphopeptide chelated calcium (CPP-Ca), which comprises the following steps: firstly, dissolving casein phosphopeptide (CPP) in ultrapure water, adding calcium chloride, adjusting the pH value, stirring and reacting at a certain temperature to generate CPP-Ca, then precipitating by using ethanol, washing for multiple times to remove free calcium ions, and drying to obtain the high-absorptivity calcium supplement of vitamin D3 (VD3) and casein phosphopeptide chelated calcium (CPP-Ca). Centrifuging, collecting the precipitate, and freeze-drying to obtain purified CPP-Ca; the preparation method comprises the following steps: preparing CPP-Ca into a solution, adjusting the pH value, dropwise adding VD3 dissolved in ethanol into the solution, stirring and mixing at room temperature under a certain concentration to finally form the VD3 and CPP-Ca compound, and the compound prepared by the method can significantly promote calcium absorption and effectively improve the stability of VD3 and CPP.

The application belongs to the technical field of biology and specifically relates to a casein phosphopeptide for promoting calcium, iron and zinc mineral absorption and improving bone health. The application obtains the casein phosphopeptide containing high-activity peptide segments by optimizing the process, and takes six mineral elements of calcium, magnesium, copper, iron, zinc and manganese as research objects. Firstly, the mineral deficiency rat model is used to evaluate the mineral utilization of the CPP. The consumption of 7mg / kg.d increases the contents of Ca, Mg, Cu, Fe, Zn and Mn in femur by 7.12%, 3.56%, 20.76%, 15.84%, 9.94% and 67.12% respectively. Secondly, the molecular docking technology is used to screen the peptide segments with calcium absorption activity from the CPP and perform function verification. The application aims to reveal the effect of the CPP in the mineral absorption and utilization from the cell and animal levels, and provide a theoretical basis for the functional development.

The invention relates to the field of hair growth nutrients, and discloses a nutrient composition for promoting hair growth and a preparation method thereof, the composition comprises a basic hair growth nutrient group and an absorption-promoting synergistic group; the basic hair growth nutrition group comprises the following components in parts by weight: 0.3-0.8 part of ferrous lactate, 2-5 parts of zinc gluconate, 5-8 parts of magnesium stearate, 3-8 parts of a coating material and 17-25 parts of milk mineral salt; the absorption-promoting synergistic group comprises the following components in parts by weight: 0.2-1.5 parts of a black pepper extract, 2-10 parts of lecithin and 2-8 parts of casein phosphopeptides, the black pepper extract and the lecithin are firstly released, an intestinal epithelium channel is opened by utilizing a biological penetration promoting effect, and a pre-permeation state is established; the subsequently released CPP is complexed with divalent metal ions in intestinal juice to prevent precipitation, so that the CPP is synchronously and rapidly absorbed through a pre-opened channel, and the problems of competitive antagonism and difficult absorption of high-dose minerals are effectively solved.

The invention provides a preparation method and application of a dairy product for promoting development of an intestinal tract glycocalyx layer. Comprising a specific dose of casein phosphopeptides, and can up-regulate the transcriptional levels of a coding gene gpc1 of phospholipid globulin glycan, a coding gene has1 of hyaluronic acid synthetase 1, a coding gene has2 of hyaluronic acid synthetase 2, a coding gene has3 of hyaluronic acid synthetase 3, a coding gene ext1 of exosome glycosyltransferase 1 and a coding gene ext2 of exosome glycosyltransferase 2. Therefore, the development of a protein skeleton of the glycocalyx layer, the synthesis of hyaluronic acid and the synthesis of heparan sulfate are promoted, the development of the glycocalyx layer is further promoted, and the effects of improving the intestinal barrier, regulating the intestinal flora and improving the intestinal health are achieved.

The present invention relates to the field of nutraceutical compositions aimed at enhancing calcium absorption. More particularly, the invention provides a bioactive composition comprising a synergistic combination of casein phosphopeptides and Parmelia perlata, in conjunction with pharmaceutically acceptable excipients. The unique formulation of these components synergistically promotes improved calcium absorption, thereby offering enhanced nutritional and therapeutic benefits.

The present application provides a solid beverage of forest frog collagen composite peptides, which contains forest frog collagen peptide powder, collagen peptides, milk mineral salts, arabinose, L-calcium lactate, ginger extract, steviol glycosides, taurine, xylitol, oligoisomaltose, maltodextrin, vitamin C, casein phosphopeptide, theanine, edible flavors and stabilizers. The forest frog collagen peptide powder and the polypeptide components in the solid beverage of the present application have the effects of anti-oxidation and promoting bone growth; calcium lactate, forest frog collagen peptide powder and milk mineral salts provide a large amount of calcium ions, which provide a good nutritional environment for bone growth. The forest frog collagen peptide powder in the solid beverage of the present application uses the residue after extraction for secondary extraction and development, realizes comprehensive utilization and promotes environmental protection. Save raw material costs.