The invention provides a method for preparing a
protease beverage by fermenting
cyperus esculentus
powder and a product thereof. Comprising the steps of directional pretreatment,
fermentation medium preparation, segmented
fermentation and optional blending, specifically, directional pretreatment adopts low-temperature
extrusion at the temperature of 60-70 DEG C and ultrasonic treatment at the power of 200-300 W for cooperative treatment, and the
fiber structure and antinutritional factors of
soybean meal are effectively broken; in the
fermentation stage,
lactobacillus plantarum,
saccharomyces cerevisiae and
bacillus coagulans composite
flora are inoculated, anaerobic fermentation is performed at the temperature of 35-37 DEG C, the pH value of the
system is reduced to 4.0-4.5, and then L-
cysteine hydrochloride-
vitamin B synergist is added for secondary fermentation in a micro-aerobic environment at the temperature of 38-40 DEG C. The fermentation process provided by the invention is simple, the treatment is convenient, the used equipment is conventional equipment in the
food industry, and the method is suitable for large-scale industrial production. Meanwhile, in the prepared fermentation liquor, the
protease activity is larger than or equal to 320 U / mL, the total number of viable
bacteria is larger than or equal to 1.2 * 10 CFU / mL, the optimum pH of the
protease is 3.8-4.8, the optimum temperature is 37-39 DEG C, the protease is highly matched with the
human gastrointestinal tract environment, and the method has wide industrialization prospects.