Lactobacillus reuteri and its application

A technology of Lactobacillus reuteri and bacteria agent, applied in the field of microorganisms, to achieve the effect of wide application prospects, reduce the area, and restore the increase of hormone levels

Active Publication Date: 2017-12-29
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, so far, there is no single strain of probiotics (such as Lactobacillus reuteri) that can be colonized in the human intestine alone to relieve metabol

Method used

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  • Lactobacillus reuteri and its application
  • Lactobacillus reuteri and its application
  • Lactobacillus reuteri and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] Example 1: Lactobacillus reuteri CCFM8631 has good tolerance to simulated gastrointestinal fluid

[0109] Streak inoculation of cryopreserved Lactobacillus reuteri CCFM8631 in MRS solid medium, anaerobic static culture at 37°C for 48 hours, and subculture 2 to 3 times in MRS medium, then take Lactobacillus reuteri The culture fluid of CCFM8631 was collected by centrifugation at 8000×g for 5 min, resuspended in (1:1) artificial simulated gastric juice (MRS medium containing 1% pepsin, pH=2.5) at pH 2.5, and incubated at 37°C For anaerobic culture, samples were taken at 3 hours, and MRS agar medium was poured for culture to count plate colonies, determine the number of viable bacteria and calculate their survival rate. The survival rate is the ratio of the number of viable bacteria when sampling in the culture solution to the number of viable bacteria at the 0th hour, expressed in %.

[0110] Take the culture solution of Lactobacillus reuteri CCFM8631, centrifuge at 8000...

Embodiment 2

[0114] Example 2: Lactobacillus reuteri CCFM8631 has no toxic side effects on SD rats

[0115] Suspend Lactobacillus reuteri CCFM8631 in 2% sucrose solution to make a concentration of 3.0×10 9 CFU / mL bacterial suspension. Take 8 healthy male SD rats with a body weight of about 180g-200g. After adapting to the environment for a week, they are given the above-mentioned concentration of bacterial suspension by gavage once a day. . The results are shown in Table 3.

[0116] Table 3 Changes in body weight and death of rats

[0117] time (days)

1

2

3

4

5

6

7

weight (g)

228.2±0.9

235.3±1.1

241.1±0.5

246.7±0.7

252.0±0.2

258.2±1.3

264.5±0.4

Circumstances of death

-

-

-

-

-

-

-

[0118] Note: -: Rats did not die

[0119] The results showed that the feeding concentration was 3.0×10 9 The suspension of Lactobacillus reuteri CCFM8631 in CFU / mL had no significant effect on rats, no significant c...

Embodiment 3

[0120] Example 3: Lactobacillus reuteri CCFM8631 has a good recovery effect on tissue damage such as liver and duodenum in rats with metabolic syndrome

[0121] Forty-eight healthy male SD rats with a body weight of 180-200 g were taken, adapted to the environment for 1 week, and randomly divided into 6 groups: blank control group (NC), high-sugar and high-fat model control group (HFHS), simvastatin control group (SC ), rosiglitazone control group (RH), Lactobacillus reuteri CCFM8631 intervention group (CCFM8631), Lactobacillus rhamnosus GG control group (LGG), each group contained 8 mice, and intragastric administration of bacteria suspension The concentration is 3.0×10 9 CFU / mL, resuspended in 2% sucrose solution. The grouping and treatment methods of the experimental animals are shown in Table 4.

[0122] Table 4 Grouping and treatment of experimental animals

[0123]

[0124]

[0125] At the end of the experiment, the rats were fasted for 12 hours, anesthetized by...

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Abstract

The invention relates to the technical field of microorganism, and discloses a Lactobacillus reuteri and its application. The preservation number of the Lactobacillus reuteri CCFM8631 is CGMCC No.14394; the level of mice peripheral neurotransmitter 5-hydroxytryptamine can be significantlyimproved; the rise of the mice peripheral blood testonsterone level and the abundant abnormity of Blautia, Turicibacter, Oscillospira and Bifidobacterium in the intestinal flora by high glucose and high fatty diets are recovered; the tolerated simulative gastrointestinal fluid is rapidly planted in an intestinal tract, so as to significantly improve the pathological injury of metabolic syndrome mice liver and duodenum and rise of triglyceride and total cholesterol content in the serum by high glucose and high fatty diets are significantly improved; the Lactobacillus reuteri can be used for preventing, delaying or treating metabolic disorder such as metabolic syndrome, irritable bowel syndrome, and anxiety, depression and other metal diseases related to irritable bowel syndrome.

Description

technical field [0001] The present invention relates to the technical field of microorganisms, in particular to a Lactobacillus reuteri and its use, especially a Lactobacillus reuteri capable of regulating intestinal flora, regulating the brain-gut axis, and significantly alleviating metabolic syndrome and its use . Background technique [0002] In recent years, with the development of the economy, the lifestyle of the people in our country has changed significantly, the amount of activity has decreased, the proportion of obesity has increased significantly, and the prevalence of diabetes and metabolic syndrome (Metabolic Syndrome) has increased significantly. Epidemiological surveys show that about 20%-30% of adults worldwide suffer from metabolic syndrome. In 2013, the results of a multi-center, multi-level stratified large-scale sampling survey organized by the Diabetes Society of the Chinese Medical Association showed that the prevalence of metabolic syndrome among peop...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C9/123A23C13/16A23C19/032A23C19/06A23C11/10A23L11/00A23L19/00A23L33/135A61K35/747A61P25/22A61P25/24A61P3/06A61P3/10A61P1/16A61P1/00C12R1/225A23L11/50
CPCA23L19/00A23L33/135A23C9/1234A23C11/106A23C13/16A23C19/0323A23C19/062C12N1/20A23V2002/00A61K2035/115A23L11/50C12R2001/225C12N1/205A23L11/65A23V2400/173A23V2200/3204A23V2200/30A23V2200/3262A23V2200/328A61P3/00A61P1/00A61K35/747
Inventor 马方励陈卫王刚朱广素王园园肖凌云胡明华张灏赵建新马忠华
Owner INFINITUS (CHINA) CO LTD
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