366 results about "Solanum tuberosum" patented technology

The invention discloses protease for improving the resistance of potatoes to late blight as well as a coding gene and application thereof, and belongs to the technical field of genetic engineering and plant disease resistance breeding. The amino acid sequence of the protease StRD21 is as shown in SEQ ID NO.2, or the protease StRD21 has a derivative sequence with the same disease-resistant function. The nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention also provides a specific primer pair for cloning the gene, a potato recombinant overexpression vector containing the gene and a transgenic host cell. The StRD21 gene is introduced into potatoes and overexpressed, so that the resistance of the potatoes to late blight can be remarkably enhanced. The invention provides an effective gene resource and a biotechnological means for cultivating a new variety of disease-resistant potatoes.

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor StBSB with a regulation effect on steroid glycoalkaloid accumulation of potatoes and application of the transcription factor StBSB, the transcription factor StBSB is encoded by a potato gene Soltu.DM.05G001620, and the nucleotide sequence of the potato gene Soltu.DM.05G001620 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor StBSB. According to the application, a new insight is provided for understanding a transcriptional regulation mechanism of SGAs in solanaceae plants, and a key theoretical basis and genetic resources are provided for directionally improving the content of anti-nutritional compounds through genetic means.

The invention discloses an arabidopsis thaliana RK3 gene and application of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene in improvement of plant disease resistance, and belongs to the technical field of plant genetic engineering. According to the application of the arabidopsis thaliana RK3 gene to improvement of plant disease resistance, the nucleotide sequence of the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.1, the amino acid sequence of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.2, and further plants are selected from arabidopsis thaliana, oilseed rape, rice, tomatoes, potatoes, peanuts, soybeans, cotton, tobacco, cucumbers, watermelons and the like. The expression of the plant disease resistance comprises infection resistance to wild-type pseudomonas syringae, effector engineering strains thereof and botrytis cinerea. It is clear that the arabidopsis thaliana RK3 gene and the encoded protein thereof can remarkably enhance the infection resistance of plants to various pathogenic bacteria for the first time, disease prevention and control are achieved by activating the autoimmune system of the plants, and an effective path is provided for reducing chemical pesticide application and promoting agricultural green development.

The invention discloses dsRNA of a targeted potato beetle heat shock protein HSP70-KDa3 gene as well as a preparation method and application of the dsRNA of the targeted potato beetle heat shock protein HSP70-KDa3 gene. In order to solve the problems that an existing RNAi target gene is prone to off-target, poor in field stability and the like, the potato beetle heat shock protein gene LdHSP70-KDa3 serves as an RNA interference target for the first time, and a double-stranded RNA molecule shown as SEQ ID NO: 1 and a preparation method for preparing the double-stranded RNA are provided. Feeding experiments prove that the double-stranded RNA can efficiently silence target genes, the survival rate of potato beetle larvae is remarkably reduced, and feeding and growth development of the potato beetle larvae are inhibited. In addition, the invention further provides a biological preparation containing the double-stranded RNA and application of the biological preparation in prevention and treatment of potato beetles. The dsRNA targeting the HSP70-KDa3 gene provided by the invention has the characteristics of high efficiency, specificity, environmental friendliness and the like, and a new technical scheme is provided for green prevention and control of potato beetles.

The invention discloses a plant immunomodulatory gene RDP as well as an expression vector and application thereof. The inventor finds a plant immunomodulatory factor gene RDP from potatoes, after a corresponding sequence is obtained through cloning, agrobacterium competent cells are transferred into nicotiana benthamiana, it is verified that the plant immunomodulatory factor gene RDP can be successfully expressed in other plants, and it is verified that the pathogenic effects of various phytophthora are good. The gene is proved to be capable of effectively improving the disease resistance of the nicotiana benthamiana after being expressed. Through objective evaluation of an agrobacterium tumefaciens-mediated transient expression system, it is found that the plant immunomodulatory factor RDP can induce plants to generate resistance, the gene can be directly introduced into the plants through a transgenic technology due to the powerful function of the gene, disease-resistant varieties are developed and cultivated, and the gene has great significance in prevention and treatment of plant diseases.

The present invention relates to diploid, fertile, self-compatible and essentially homozygous potato lines, wherein said lines comprise an agronomically desirable trait such as vigour. The invention further relates to methods for producing such plants and to hybrid seeds obtained by crossing such homozygous potato lines and to potato plants grown from said seed.

The invention discloses application of a potato transcription factor StBBM gene in potato tuberization breeding, and aims to regulate formation and development of potato tubers through silencing and overexpression of the StBBM gene so as to improve the yield and quality of the potato tubers. Through overexpression of the StBBM gene, the tuberization efficiency of the potatoes is remarkably improved, the tuberization time is advanced, early maturing is promoted, and the planting period is shortened. In addition, the tuber shape for silencing the gene is changed from an ovulate shape to a long cylinder shape, and the weight of a single potato plant is reduced, so that the storage and transportation efficiency can be improved, the market preference can be met, more choices are provided for breeding, and the cultivation of potato varieties adapting to different conditions is facilitated. Generally, the present invention enhances the production and adaptability of potatoes and brings advantages to the entire production chain of potatoes by improving the form and weight of tubers.

The invention provides a self-propelled potato combine harvester with a residual film recovery device, belongs to the technical field of agricultural machinery, and solves the problems of high soil carrying amount, residual film winding, large potato block damage and the like in film-covered potato harvesting. The machine comprises a main machine frame, a crawler belt walking mechanism, a power system and a harvesting mechanism, a secondary soil shaking conveying mechanism, a secondary soil shaking screening mechanism, a lifting conveying mechanism, a cleaning conveying mechanism and a bag hanging mechanism which are arranged in the advancing direction, and a continuous operation chain is formed. The core is that the harvesting mechanism is integrated with a residual film recovery device, and the front recovery of the residual film is realized through film guiding, film pressing, reciprocating film pulling and winding. The whole machine is of a multi-stage soil shaking and screening and low-damage conveying structure, a power system achieves power distribution and speed regulation through a hydraulic electromagnetic valve set, and all the mechanisms are assembled in a modularized mode to adapt to different soil textures and ridge types. The potato harvester integrates harvesting and residual film recycling, is high in potato cleaning rate, low in potato block damage, high in continuous operation capacity and convenient to maintain, and meets the large-scale and green potato production requirements.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses application of an important member of a potato mitogen-activated protein kinase (MAPK) family, namely an StMKK9 gene in regulation and control of maturity and cold resistance of potatoes. CDNA of a cultivar Desiree leaf is used as a template, the potato StMKK9 gene is separated and cloned, an over-expression vector is constructed, and the over-expression vector is used for expression of the potato. Genetic transformation of the potatoes is further carried out, it is found that the overexpressed StMKK9 gene can promote prematurity of the potatoes, meanwhile, the cold resistance of potato plants can be improved, and a new genetic material and a theoretical basis are provided for analysis of a potato tuberization molecular mechanism and improved breeding of the potatoes.

The invention provides application of a potato StMYB4 gene in regulation and control of solanine accumulation in potato tubers, the StMYB4 gene is cloned through a sequence published in a potato genome database by taking cDNA of leaves of cultivated species Hubei potato No.3 and AC142 as a template, an overexpression and knockout vector is constructed, genetic transformation is performed on the Hubei potato No.3 and AC142 respectively for transgenic function verification, and the solanine accumulation in the potato tubers is regulated and controlled. It is found that excessive StMYB4 genes can significantly promote synthesis of solanine in potato tubers, and knockout of the StMYB4 genes can significantly inhibit synthesis of solanine in potato tubers. The application of the potato StMYB4 gene to regulation and control of tuber solanine can be used for molecular marker development and low solanine variety breeding.

The invention discloses a method for increasing the yield of monoterpene glycoside in an endophytic fungus fermentation product separated from paeonia lactiflora, and relates to the technical field of plant fermentation. The method comprises the following steps: material treatment; separating strains; identifying strains; preparing a strain and an elicitor: drying and crushing the overground part of the Chinese herbaceous peony by using the endophytic fungus ER6 separated and identified in the step S3, and sieving with a 80-mesh sieve to prepare powder as a key elicitor; a potato glucose liquid culture medium is used as a basic fermentation culture medium, an activated ER6 strain is inoculated into a fermentation container filled with a sterilized PDB culture medium, and dry powder of the overground part of paeonia lactiflora is added into the culture medium to serve as a biological elicitor to be cultured in a shaking table; and harvesting fermentation liquor. According to the method, a specific endophytic fungus ER6 is separated from paeonia lactiflora roots, and it is found that the yield of monoterpene glycoside in a fermentation product can be remarkably increased by using the overground part of paeonia lactiflora as a biological elicitor to ferment the endophytic fungus ER6.

This invention discloses the application of a compound microbial agent S14 in improving the salt tolerance and promoting the growth of potatoes, belonging to the field of microbiology. The compound microbial agent S14 is composed of three bacteria: Lelliottia sp. 132, Leucobacter tardus 153, and Pantoea ananatis 103. This compound microbial agent can effectively promote potato growth and significantly increase the emergence rate, biomass, plant height, and tuber weight of potatoes. The compound microbial agent S14 can not only effectively maintain the ion content of hybrid diploid potato seedlings, but also effectively improve the salt tolerance of potatoes, thereby increasing the tuber weight of the commercial variety Fiurita in saline-alkali soil. The compound microbial agent S14 provided by this invention can improve the salt tolerance of potatoes and is suitable for potato cultivation in saline-alkali soil, belonging to the field of plant rhizosphere growth-promoting bacteria technology.

The invention discloses a potato late blight antagonistic bacterium and application thereof, belongs to biological agents, and provides more strain resources for prevention and treatment of potato late blight. The potato late blight antagonistic bacterium is Pseudomonas chlororaphis blT-49 and is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 27, 2021, and the preservation number is CGMCC No.24203. The invention further discloses a preparation method of the potato late blight antagonistic bacterium. The strain disclosed by the invention not only can inhibit the occurrence and growth of potato late blight, but also can regulate and control the ecological system of the micro-ecology of potatoes, reduce the infection ability of potato late blight and improve the disease resistance of potatoes. In the growth and development of the potatoes, the growth and development of the potatoes can be promoted, and the single-plant yield is improved.

The invention discloses a factory-like efficient breeding method and system for virus-free seed potatoes in a potato bottle, and relates to the technical field of agricultural biology. The method comprises the following steps: selecting healthy buds, stripping stem tips, carrying out primary culture to obtain seedlings, screening virus-free seedlings through RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection, and transferring the virus-free seedlings to a culture flask containing a liquid MS culture medium for expanding propagation; hardening the propagated virus-free seedlings to obtain stress-resistant virus-free seedlings, inoculating the stress-resistant virus-free seedlings into a tissue culture bottle containing an induction culture medium, performing induction culture under set illumination and temperature, and dynamically adjusting a nutrient solution to obtain virus-free seed potatoes; and classifying and screening the detoxified seed potatoes and storing in a specific environment. The system comprises a sterile operation unit, a multi-stage culture unit, a seedling hardening unit and a graded storage unit. The method can efficiently breed the virus-free potato seeds, reduces the virus infection risk, improves the breeding efficiency and the potato seed quality, and is suitable for industrialized production.

The present application relates to the technical field of fertilizer preparation, and particularly relates to potato residue fermentation compound fertilizer with plant disease prevention and treatment effect and application thereof.The present application provides potato residue fermentation compound fertilizer with plant disease prevention and treatment effect, which comprises potato residue fermentation organic fertilizer and traditional Chinese medicine compound preparation, and the mass ratio is 90-110:11-17.The present application uses traditional Chinese medicine compound preparation in combination with potato residue fermentation organic fertilizer, and the addition of traditional Chinese medicine compound preparation not only further improves the fertilizer efficiency of the fertilizer, but also makes the fertilizer have the effect of preventing and treating plant diseases, so that the dual effects of promoting growth and preventing diseases are achieved.

The invention discloses a composition for repelling aphids and preventing potato Y virus infection and a virus and insect inhibitor, and belongs to the technical field of plant disease prevention and control. The pharmaceutical composition provided by the invention is prepared from at least two of 1-pentene-3-ketone, 2-ethyl furan and cis-4-heptenal, and the pharmaceutical composition is prepared from at least two of 1-pentene-3-ketone, 2-ethyl furan and cis-4-heptenal. The pharmaceutical composition not only can avoid aphids and reduce the probability that crops are infected by potato Y viruses, but also can realize the synergistic interaction among the drugs and avoid or reduce the drug resistance of the aphids to the drugs, so that the pharmaceutical composition has important practical value and application prospect in the technical field of plant disease control.

The invention discloses a compound microorganism fermented attenuated aroma-enhanced potato residue feed and a preparation method thereof, and belongs to the field of feed processing. The feed comprises potato residues, crop straw particles, corn flour, bran, soybean meal, urea, ammonium sulfate and a compound microbial agent, wherein the compound microbial agent comprises bacillus velezensis and lactobacillus plantarum. The preparation method comprises raw material pretreatment; preparing a microbial agent; performing mixed fermentation; and post-processing. The bacillus velezensis secretes glycosidase, and glucosidic bonds of the glycoalkaloid are subjected to enzymolysis; lactobacillus plantarum is metabolized to generate lactic acid to create an acidic environment to promote chemical degradation of glycoalkaloid, and the double bacteria cooperate to inhibit growth of mould, so that efficient attenuation is realized. The bacillus velezensis metabolizes to generate glucose, ethanol and free amino acid, and the lactobacillus plantarum metabolizes to generate ester flavor substances, so that the feed is endowed with strong fermentation aroma. Meanwhile, the pungent smell of volatile aldehyde substances is eliminated through double-bacterium synergism.

The invention discloses a fermentation medium for high yield of oudemansiella oosporium mycelium polysaccharide and a culture method thereof. The fermentation medium comprises the following components in percentage by mass: 20-25% of potatoes, 1-4% of corn flour, 0.2-0.4% of peptone, 0.2-0.4% of CaSO4, 0.05-0.15% of MgSO4 and the balance of water, and the pH value of the fermentation medium is 5.5-6.5. According to the culture medium, growth of Oudemansiella ovalis hyphae can be accelerated by regulating and controlling the proportion of different nutritional components, synthesis of Oudemansiella ovalis polysaccharide can be promoted, the final sugar yield of the strain is increased by 7 times compared with that of conventional culture by adopting the optimized fermentation culture medium and fermentation process, and the sugar yield can reach 167.37 mg / g. The scavenging rates of DPPH free radicals, ABTS free radicals and hydroxyl free radicals of the oospore oudemansiella sp. Polysaccharide are 95.02%, 98.08% and 93.74% respectively, and the scavenging rates are obviously higher than those of previous research results. The invention provides a new direction for developing natural antioxidants, and also provides theoretical significance and application value for developing oudemansiella oosporium mycelium fermentation products.

This utility model discloses a potato tuber peeling machine, including a peeling box. Inside the peeling box, six correspondingly distributed rotating shafts are rotatably connected. Each of the six rotating shafts is fixedly connected to a brush roller, and each of the six rotating shafts is also fixedly connected to a transmission gear. The teeth of the six transmission gears mesh with each other. A placement plate is fixedly connected to the outside of the peeling box, and a first motor is fixedly connected to the top of the placement plate. The output end of the first motor is fixedly connected to one of the transmission gears. A moving cleaning mechanism is provided at the top of the peeling box, and a lifting and unloading mechanism is provided at the bottom of the peeling box. The moving cleaning mechanism allows for convenient and even movement and cleaning of the potatoes, ensuring they are thoroughly cleaned during the peeling process for convenient subsequent use. The lifting and unloading mechanism allows for easy tilting of the peeling box, facilitating the unloading of the peeled potatoes.

The present application relates to the technical field of plant breeding, in particular to StERF1A Application of a gene related biological agent in improving the resistance of potato to bacterial wilt, the biological agent StERF1A The nucleotide sequence of the gene is shown as SEQ ID NO. 1. The present application discloses for the first time StERF1A As a negative regulatory factor involved in the mechanism of potato resistance to bacterial wilt: overexpression StERF1A Can increase the accumulation amount of bacterial wilt in leaves, inhibit the expression of disease resistance related genes Pto 、 PR1b1 And PR1a The content of active oxygen ROS and the activity of related enzymes SOD, POD and CAT are reduced; and the expression of down-regulation StERF1A The opposite effect is presented, which significantly enhances the resistance of potato to bacterial wilt. The present application provides an important gene target and theoretical basis for breeding new potato varieties with high resistance to bacterial wilt.

The invention provides an SNP (Single Nucleotide Polymorphism) site and method for identifying the trehalose content of a tetraploid potato tuber and application, the physical position of the SNP site is determined on the basis of sequence comparison of a chromosome 12 of a tetraploid potato'Qing potato No.9 'reference genome, and the SNP site comprises: an A2 haplotype SNP single nucleotide polymorphism site snp: 35849480, the polymorphism being A / G; the SNP single nucleotide polymorphism site snp of the A3 haplotype is 55777825, and the polymorphism is T / G; the SNP single nucleotide polymorphism site snp of the A4 haplotype is 46535012, and the polymorphism is A / G; the SNP single nucleotide polymorphic site snp of the A4 haplotype is 46568448, and the polymorphism is A / T. The invention provides an important molecular tool for efficient identification and genetic improvement of the trehalose content of the tetraploid potatoes.

The utility model belongs to the technical field of potato seeding machinery, and particularly relates to a plot potato seeder seeding depth regulation and control chassis which comprises a front rack, a supporting land wheel assembly, a seeding depth regulation and control device, a driving land wheel assembly and a rear rack, the front rack is composed of a left longitudinal beam, a right longitudinal beam and a front cross beam, the two ends of the left longitudinal beam and the two ends of the right longitudinal beam are connected with the front cross beam and the rear cross beam through U-shaped clamps respectively, and the rear cross beam is fixed to the rear rack through welding. Supporting land wheel assemblies are mounted on the left and right sides of the front cross beam; the middle part of the sowing depth regulating device is connected with the rear cross beam through a breadth regulating square pipe, and the bottom end is connected with the driving land wheel box through a supporting plate. Before operation, the operation requirements of different ridge widths can be met by adjusting the distance between the supporting land wheel assemblies and the driving land wheel assemblies on the left side and the right side; the height from the rack to a ridge surface can be adjusted by rotating the supporting land wheel assembly and a hand wheel on the seeding depth adjusting and controlling device, so that the seeding depth is adjusted. The seeder chassis has the characteristics of being simple in structure, convenient to adjust and suitable for operation in different terrains.

This invention relates to the field of potato sprouting technology, specifically to a potato sprouting planter and planting method. The planter includes: a frame; a potato tuber seed box mounted on the frame; a seed dispensing disc located below the outlet of the potato tuber seed box to receive the tubers inside; the seed dispensing disc intermittently dispensing tubers during its rotational motion; a tilting bucket capable of tilting and tilting, containing a rotating disc and a lifting seed guard bucket located outside the rotating disc; and a planting channel located to the side and below the tilting bucket. This invention achieves automatic adjustment of the bud orientation of the potato tubers during the process of falling from the seed box until planting is completed in the planting channel, through the coordinated operation of the rotating disc and the lifting seed guard bucket. Furthermore, the tilting bucket and planting channel maintain the adjusted posture of the tubers as they fall into the pre-drilled trench, ensuring stable bud orientation.

The invention discloses a lentinus edodes cultivation method based on liquid strain inoculation. The lentinus edodes cultivation method comprises the following steps: S1, preparing raw materials of a lentinus edodes cultivation compost formula; s2, preparing fungus bags: uniformly mixing the culture materials according to a formula, bagging, sterilizing in a sterilization chamber at normal pressure, taking out and cooling for later use; s3, preparing liquid strain formula raw materials: 10 to 30 parts of potatoes, 2 to 5 parts of agar, 2 to 5 parts of cane sugar, 0.02 to 0.05 part of citric acid and 500 to 700 parts of water; s4, preparing a liquid strain, namely boiling potatoes in boiling water, taking juice, adding cane sugar and citric acid into the juice, sufficiently and uniformly stirring, sterilizing the mixed solution, and culturing in a constant-temperature room, so as to obtain the mushroom liquid strain; s5, inoculating the liquid strain prepared in the step S4 to the fungus bag prepared in the step 2; s6, the fungus bags obtained in the step S5 are placed in a constant-temperature culture room to be cultured, and S7, after the fungus bags obtained in the step S6 are subjected to dark culture, normal fruiting can be achieved.

The invention relates to the technical field of biology, and discloses a potato gene StVac14 and application thereof in regulation and control of drought resistance of potatoes. According to the invention, the StVac14 gene is knocked out, so that the important function of the StVac14 gene in regulating the drought resistance of the potatoes is disclosed. The knockout of the StVac14 gene significantly reduces the drought resistance of potatoes, and the mutant shows more obvious drought sensitivity than a wild type. The invention provides a method for optimizing the drought resistance of potatoes by regulating the StVac14 gene through a gene editing technology, and the method has a wide breeding application prospect.

The application provides a method for identifying potato drought-resistant genes using hyperspectral, and relates to the technical field of crop drought-resistant gene identification, and comprises the following steps: setting a drought group and a control group, synchronously acquiring plant canopy hyperspectral images and transcriptome data and establishing a correlation data set; extracting spectral features, screening drought response candidate genes; calculating the correlation coefficient of each candidate gene and the spectral features based on Spearman correlation analysis, and determining the primary gene set according to whether the average value of a plurality of positions after the absolute value is sorted exceeds a threshold value; constructing a random forest regression model with the spectral features as input and the expression amount of the primary gene as output, predicting the expression amount through a verification sample, and screening target drought-resistant genes according to the coefficient of determination and the prediction error; constructing a target gene overexpression plant, measuring the relative change rate of physiological indexes and the change multiple of gene expression amount under drought, calculating a comprehensive function verification index, and determining the gene as a drought-resistant gene if the index exceeds a threshold value.

This invention relates to the field of medicinal chemistry, specifically to a capsaicin-like compound containing isopropanolamine, its preparation method, and its application. This type of compound has a structure as shown in general formula (I). The invention uses capsaicin and isopropanolamine as pharmacophores, connecting the two pharmacophores with different bridging chains to synthesize a series of capsaicin-like derivatives containing isopropanolamine. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as rice bacterial blight, citrus canker, rice bacterial leaf streak, and kiwifruit canker; and plant pathogenic fungi such as cucumber gray mold, pepper wilt, rapeseed sclerotinia rot, eggplant verticillium wilt, wheat scab, potato late blight, blueberry root rot, grape coccidioidomyces, dragon fruit anthracnose, and rice sheath blight, providing an important scientific basis for the research and development of new pesticides.

The application provides a method for planting white mushroom on Smith pine stumps, and relates to the technical field of artificial planting of white mushroom. The method for planting white mushroom on Smith pine stumps comprises the following steps: sterilizing old stumps / new stumps after felling of Smith pine by using a blowtorch, pouring a water solution of extract of salt water of drunken fish grass, and inoculating liquid spawn of white mushroom after air-drying, wherein the liquid spawn of white mushroom is obtained by using a mixture of almond paste, extract of salt water of drunken fish grass and potato liquid medium as a culture medium. The application overcomes the shortcomings of the prior art, effectively promotes the growth of white mushroom on the stumps by sterilizing the stumps and using the nutrient agent, adjusts the culture medium components of the white mushroom, increases the yield, and improves the economic benefits.