907 results about "Solanum tuberosum" patented technology

The invention belongs to the technical field of chlorophyll content inversion, and discloses a canopy chlorophyll content inversion method based on feature extraction and a machine learning model, and the method comprises the following steps: employing a mode of combining an unmanned plane carrying a multispectral camera and ground actual measurement; collecting and processing the chlorophyll content data of the potato canopies, and planting a quilt index; carrying out feature selection by using a Pearson correlation analysis and competitive self-adaptive reweighted sampling algorithm; constructing a chlorophyll content inversion model based on machine learning; and carrying out chlorophyll content inversion model optimization based on a grey wolf optimization algorithm and a sparrow optimization algorithm, and carrying out precision evaluation. According to the canopy chlorophyll content inversion method based on feature extraction and the machine learning model, comparative study is carried out on the single growth period and the whole growth period of potatoes, the optimal inversion model suitable for the single growth period and the whole growth period is determined, and the accuracy and applicability of the study are guaranteed.

The invention discloses a nutrient solution concentration regulation and control method and system for potato aeroponic cultivation, and relates to the technical field of fertilization, and the method comprises the following steps: extracting root feature data and concentration feature data; establishing a concentration trend prediction model based on the root feature data and the concentration feature data, and predicting the concentration change trend of the nutrient solution under different conditions by using the concentration trend prediction model; analyzing the change trend of the nutrient solution concentration under different conditions by using a feature analysis algorithm, and identifying key factors influencing the stability of the nutrient solution concentration; and based on the key factors, the nutrient solution is dynamically prepared. Key factors influencing the concentration stability are identified through a feature analysis algorithm, and the nutrient solution ratio is dynamically regulated and controlled, so that intelligent management and accurate supply of the nutrient solution concentration are realized, and effective support is provided for nutrition optimization of the potato aeroponic culture whole period.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention discloses protease for improving the resistance of potatoes to late blight as well as a coding gene and application thereof, and belongs to the technical field of genetic engineering and plant disease resistance breeding. The amino acid sequence of the protease StRD21 is as shown in SEQ ID NO.2, or the protease StRD21 has a derivative sequence with the same disease-resistant function. The nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention also provides a specific primer pair for cloning the gene, a potato recombinant overexpression vector containing the gene and a transgenic host cell. The StRD21 gene is introduced into potatoes and overexpressed, so that the resistance of the potatoes to late blight can be remarkably enhanced. The invention provides an effective gene resource and a biotechnological means for cultivating a new variety of disease-resistant potatoes.

The invention discloses a ridging, film mulching, hole sowing and soil covering all-in-one machine for potato breeder's seeds, and relates to the technical field of hole sowing of the potato breeder's seeds. A ridging mechanism is arranged at the bottom of the all-in-one machine body, and the ridging mechanism comprises a ridging soil turning plate and a ridging rotating wheel; a seeding mechanism is arranged on the left side of the bottom of the all-in-one machine body and comprises a seeding hopper, a fertilizer storage hopper and a water supply storage hopper; wherein a film covering mechanism is arranged on the right side of the bottom of the all-in-one machine body, and the film covering mechanism comprises a film releasing rotating shaft. According to the ridging, film mulching, hole sowing and soil covering all-in-one machine for the potato breeder's seeds, ridging and hole pressing operations of the potato breeder's seeds are achieved through movement of the all-in-one machine body, synchronous fertilization and water supply irrigation are achieved when the potato breeder's seeds are sown, and meanwhile hole opening mulching film outer covering is conducted after ridging is conducted; soil is conveyed by the soil shifting overturning spoon to press and cover the mulching film, so that the heat-preservation and moisture-preservation effects are improved.

The invention relates to the technical field of gene isolation and plant improvement, in particular to a late blight resistance gene, a biological material and application. The Rpi-card1 nucleic acid molecule provided by the invention comprises a nucleotide sequence as shown in any one of SEQ ID NO: 1-4; a nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 5 or 6; the nucleotide sequence has at least 75% sequence identity with the nucleotide sequence. The invention provides an important gene resource for potato late blight resistance breeding, overcomes the problem of disease resistance loss of potato varieties, and provides a new guarantee for lasting disease resistance of potatoes.

The invention discloses a potato cold resistance identification method and system, and relates to the technical field of cold resistance identification. The method comprises the following steps: cultivating potato tissue culture seedlings in a standardized manner, and randomly dividing the seedlings into a low-temperature stress group and a normal-temperature control group; performing standardized low-temperature treatment including slow descent and constant-temperature stages on the stress group; the method comprises the following steps: acquiring multi-source data such as chlorophyll fluorescence parameters, canopy thermal imaging, digital image phenotypes and biochemical indexes, and forming a unified data set after derivative index calculation and standardized fusion; inputting the fusion data into an improved neural network model, training the model through a particle swarm optimization algorithm, and outputting a quantitative potato cold resistance index; automatically evaluating the cold resistance grade according to the potato cold resistance index and generating an identification report; standardization of the identification process, fusion analysis of multi-dimensional indexes and intelligent result analysis are achieved, and the accuracy, efficiency and practicability of potato cold resistance identification are remarkably improved.

The invention belongs to the field of disease and pest recognition, and provides a potato disease recognition method based on improved YOLOV8. Firstly, early blight, late blight and healthy leaves of potatoes are collected, and then image preprocessing and feature region labeling are carried out; secondly, replacing an original backbone network with an OfficientViT structure, and introducing an SCSA collaborative attention module into an initial model network structure to optimize a YOLOV8 network model; secondly, training the optimized potato disease recognition model on the basis of a PyTorch framework, and setting proper model parameters and training times to enable the trained model to achieve higher recognition accuracy through verification; and finally, deploying the constructed potato disease identification model on a mobile terminal APP to realize real-time identification of field potato diseases, so that the invention provides a method capable of accurately and rapidly identifying potato diseases, which is beneficial to improving agricultural production efficiency and crop yield.

The application discloses an NLR plug-in gene for improving plant disease resistance and application thereof, and belongs to the field of plant molecular biology and biotechnology.The NLR plug-in gene StEM1 has a whole gene sequence as shown in SEQ ID NO.1 and a CDS sequence as shown in SEQ ID NO.2, and the amino acid sequence of the encoded protein is as shown in SEQ ID NO.3.The gene plays a key role in improving plant disease resistance, especially resistance to field late blight variant bacteria.In the NLR background, overexpression of the gene significantly promotes the disease resistance of tobacco and potato to pathogenic pythium, and the gene is a gene for enhancing plant disease resistance by improving ETI immunity.Overexpression of the gene in the NLR background is obtained by a genetic transformation method, and a strain line is obtained to resist field variant strains, so as to cope with field diseases of crops.The application provides an excellent gene resource for green prevention and control of late blight and disease-resistant genetic engineering breeding.

The invention discloses a potato seedling grading environment parameter dynamic optimization regulation and control method, and relates to the technical field of agricultural informationization. Based on an Internet of Things sensor deployed in a target area, stem diameter, chlorophyll content and plant height data of potato seedlings are collected, the seedlings are divided into weak seedlings, middle seedlings and strong seedlings by adopting K-means clustering, and the weak seedlings, the middle seedlings and the strong seedlings are classified into a weak seedling classification model, a middle seedling classification model and a strong seedling classification model; setting the duration of the initial light period; and carrying out gradient photoperiod test on each type of potato seedlings by taking the stem elongation rate and the chlorophyll content as constraint conditions. Through grading photoperiod modeling and ant colony algorithm dynamic optimization, the limitation of traditional fixed photoperiod regulation and control is broken through, the initial photoperiod range is set based on the seedling physiological difference, and the photoperiod-growth rate response curved surface model is constructed in combination with the stem elongation rate and chlorophyll content constraint conditions. The influence of different photoperiod combinations on seedling growth is quantified, accurate matching of photoperiods and seedling requirements is ensured, excessive growth or premature senility is avoided, and the growth rhythm stability and the resource utilization efficiency are improved.

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor StBSB with a regulation effect on steroid glycoalkaloid accumulation of potatoes and application of the transcription factor StBSB, the transcription factor StBSB is encoded by a potato gene Soltu.DM.05G001620, and the nucleotide sequence of the potato gene Soltu.DM.05G001620 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor StBSB. According to the application, a new insight is provided for understanding a transcriptional regulation mechanism of SGAs in solanaceae plants, and a key theoretical basis and genetic resources are provided for directionally improving the content of anti-nutritional compounds through genetic means.

The invention belongs to the technical field of food microorganisms, and particularly discloses a ganoderma lucidum mutant strain capable of producing polysaccharides and triterpenoids at high yield. The strain is named as Ganoderma lucidum in Latin, the preservation number is CGMCC NO: 41680, the preservation unit is China General Microbiological Culture Collection Center, and the preservation date is December 4, 2024. The strain is named as Ganoderma lucidum, the preservation number is CGMCC NO: 41680, and the preservation number is CGMCC NO: 41680. The polysaccharide content in the ganoderma lucidum spore powder produced by the ganoderma lucidum mutant strain is more than or equal to 8%, and the total triterpenoid content is more than or equal to 12%; a culture medium formula of the ganoderma lucidum mutant strain comprises potato leachate, glucose and monopotassium phosphate, the culture temperature is 25-28 DEG C, and the pH value is 5.5-6.5. The ganoderma lucidum mutant strain is high in genetic stability, the content of active ingredients is remarkably higher than that of parent strains, and the ganoderma lucidum mutant strain is suitable for large-scale fermentation production.

The invention belongs to the technical field of gene engineering, and relates to application of a potato late blight bacterium response gene StRTP5 in regulation and control of potato disease resistance. The invention provides application of a potato late blight response gene StRTP5 in regulation and control of potato disease resistance, the potato late blight response gene StRTP5 comprises a gene StRTP5a and a gene StRTP5b, the gene StRTP5a and the gene StRTP5b negatively regulate and control resistance of potatoes to phytophthora infestans and alternaria solani, and the gene StRTP5a and the gene StRTP5b have potential to be used for creating potato broad-spectrum disease-resistant materials.

The invention relates to the field of molecular cytogenetics and potato ploidy breeding, in particular to a potato ploidy identification method for marking and counting chromosome centromere based on a potato CENH3 antibody and a ploidy detection kit. According to the method, the chromosome centromere is marked by specifically recognizing the antibody of the potato centromere nucleosome core histone CENH3 and combining the immunofluorescence technology, so that the accurate counting of the chromosome centromere is realized at the single cell level, and the ploidy of potatoes is judged. The method comprises the following steps: preparing a leaf cell suspension, sequentially incubating a potato CENH3 antibody and a labeled secondary antibody after immobilization, and observing and counting by a microscope after redyeing. The method has the advantages of high species specificity, high throughput and low cost, is not limited by special material taking parts of root tips or shoot tips and cell division periods, can accurately identify the uploid and aneuploid of potatoes, and provides an efficient tool for potato germplasm resource evaluation and cross breeding.

The invention discloses a monitoring analysis system and method for potato breeding, and relates to the technical field of agricultural information technology and intelligent breeding, and the system comprises a multi-modal data acquisition module which is configured to synchronously obtain greenhouse environment parameters, potato plant multispectral imaging data and leaf metabolite concentration data; and the space-time causal modeling module is connected to the multi-modal data acquisition module, and is used for constructing a dynamic correlation model among environmental fluctuation, gene expression and phenotypic characteristics, and deducing and quantifying the genetic effect of the environmental abnormal event based on anti-fact intervention. According to the monitoring analysis system and method for potato breeding, through multi-modal data fusion and space-time causal modeling, the accuracy and early warning timeliness of potato breeding monitoring are improved, the limitation of traditional visible light imaging is broken through, and the incubation period disease recognition accuracy is improved compared with a traditional method.

The invention discloses an arabidopsis thaliana RK3 gene and application of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene in improvement of plant disease resistance, and belongs to the technical field of plant genetic engineering. According to the application of the arabidopsis thaliana RK3 gene to improvement of plant disease resistance, the nucleotide sequence of the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.1, the amino acid sequence of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.2, and further plants are selected from arabidopsis thaliana, oilseed rape, rice, tomatoes, potatoes, peanuts, soybeans, cotton, tobacco, cucumbers, watermelons and the like. The expression of the plant disease resistance comprises infection resistance to wild-type pseudomonas syringae, effector engineering strains thereof and botrytis cinerea. It is clear that the arabidopsis thaliana RK3 gene and the encoded protein thereof can remarkably enhance the infection resistance of plants to various pathogenic bacteria for the first time, disease prevention and control are achieved by activating the autoimmune system of the plants, and an effective path is provided for reducing chemical pesticide application and promoting agricultural green development.

The invention relates to a potato leaf disease area positioning method, system and device.The method is applied to the potato leaf disease area positioning device.The method comprises the steps that a target infrared image of a potato leaf is segmented into a normal area and an abnormal area according to the environment temperature through a disease segmentation model obtained in advance; determining the water content of the target infrared image according to the leaf spectrum information through a pre-obtained water content prediction model; the leaf emissivity characteristic is calculated according to the water content through a pre-obtained temperature correction model, the temperature of the abnormal area is corrected based on the emissivity characteristic and the water content characteristic, and the temperature correction model is obtained based on potato leaf characteristic training; and through a pre-trained disease identification model, identifying a disease area from the abnormal area after temperature correction.

Disclosed are a Solanum tuberosum drought tolerance-related transcription factor gene StPIF4 and the use thereof. A CDS sequence of StPIF4 has a length of 1554 bp, has a nucleotide sequence shown as SEQ ID No. 1, and encodes 517 amino acids, the amino acid sequence being shown as SEQ ID No. 2. The gene is located in a cell nucleus. The gene reduces the water loss of Solanum tuberosum seedlings under the drought stress by adjusting the opening degree of stomas, thus enhancing the drought tolerance of Solanum tuberosum. The present invention provides a new genetic material and a theoretical basis for analyzing the drought-tolerant molecular mechanism and drought-tolerant breeding improvement of Solanum tuberosum.

The invention discloses a high-water-permeability potato virus-free breeder's seed efficient breeding substrate and a preparation method thereof, and relates to the technical field of agricultural cultivation. The soil conditioner is prepared from the following raw materials in parts by mass: 25-35 parts of modified coco coir, 10-15 parts of blast furnace slag, 15-20 parts of perlite, 15-20 parts of nano diatomite, 10-15 parts of straw charcoal, 3-5 parts of a polyacrylamide water-retaining agent, 1-2 parts of a compound microbial agent, 5-8 parts of tourmaline powder, 8-12 parts of attapulgite, 5-10 parts of desulfurized gypsum, 10-16 parts of vermiculite, 1-2 parts of a seaweed extract and 5-8 parts of nano zeolite. Through gradient design of the particle size of the slag, the pH value of the soil can be effectively adjusted, the resistance of the soil to acid rain can be remarkably enhanced, and the slag with different particle sizes can gradually release minerals such as calcium and magnesium, so that the stability of the pH value of the soil is maintained, and favorable conditions are provided for healthy growth of plants.

The invention discloses dsRNA of a targeted potato beetle heat shock protein HSP70-KDa3 gene as well as a preparation method and application of the dsRNA of the targeted potato beetle heat shock protein HSP70-KDa3 gene. In order to solve the problems that an existing RNAi target gene is prone to off-target, poor in field stability and the like, the potato beetle heat shock protein gene LdHSP70-KDa3 serves as an RNA interference target for the first time, and a double-stranded RNA molecule shown as SEQ ID NO: 1 and a preparation method for preparing the double-stranded RNA are provided. Feeding experiments prove that the double-stranded RNA can efficiently silence target genes, the survival rate of potato beetle larvae is remarkably reduced, and feeding and growth development of the potato beetle larvae are inhibited. In addition, the invention further provides a biological preparation containing the double-stranded RNA and application of the biological preparation in prevention and treatment of potato beetles. The dsRNA targeting the HSP70-KDa3 gene provided by the invention has the characteristics of high efficiency, specificity, environmental friendliness and the like, and a new technical scheme is provided for green prevention and control of potato beetles.

The invention belongs to the technical field of gene engineering, and relates to application of glutaredoxin StGRX5 in plant disease resistance. The invention provides an application of glutaredoxin StGRX5 in the disease resistance of plants. The glutaredoxin StGRX5 is silenced in potato plants, and the resistance of the potato plants to phytophthora infestans can be enhanced.

The invention discloses a plant immunomodulatory gene RDP as well as an expression vector and application thereof. The inventor finds a plant immunomodulatory factor gene RDP from potatoes, after a corresponding sequence is obtained through cloning, agrobacterium competent cells are transferred into nicotiana benthamiana, it is verified that the plant immunomodulatory factor gene RDP can be successfully expressed in other plants, and it is verified that the pathogenic effects of various phytophthora are good. The gene is proved to be capable of effectively improving the disease resistance of the nicotiana benthamiana after being expressed. Through objective evaluation of an agrobacterium tumefaciens-mediated transient expression system, it is found that the plant immunomodulatory factor RDP can induce plants to generate resistance, the gene can be directly introduced into the plants through a transgenic technology due to the powerful function of the gene, disease-resistant varieties are developed and cultivated, and the gene has great significance in prevention and treatment of plant diseases.

The invention provides an application of potato laccase StLAC19 in a shade avoidance reaction. The overexpression of the StLAC19 gene can significantly enhance the shading tolerance of potato plants, and effectively improve the lignin content, the osmotic regulation substance content, the chlorophyll content, the stalk strength, the CAT and POD enzyme activity and the PRO and MDA content; on the contrary, interference on expression of the StLAC19 gene results in a contrary result. The result shows that the StLAC19 gene positively regulates and controls the shading reaction of potato plants. The screening of varieties with high expression of the StLAC19 gene is helpful for obtaining materials with stronger shade-avoiding tolerance, so that the gene can be used as a target gene for breeding shade-avoiding tolerance type potato varieties.

The invention discloses a bacterial strain for antagonizing multiple pathogenic fungi of potatoes and simultaneously promoting growth and application of the bacterial strain, and relates to the field of microbial agents, the bacterial strain is bacillus wiedmannii Bw34, the bacterial strain is preserved in China General Microbiological Culture Collection Center, the preservation address is No. 3, No.1 yard, Beichen West Road, Chaoyang District, Beijing, the preservation date is November 11, 2024, and the preservation number is CGMCC NO. The preservation number is CGMCC (China General Microbiological Culture Collection Center) The compound is used for preparing a fungicide for antagonizing potato pathogenic fungi and a fungicide for promoting potato growth. The Bw34 has the effect of comprehensively preventing and treating potato soil-borne diseases, meanwhile, potato growth and nutrient absorption are promoted, and the application prospect is good.

The invention relates to the technical field of microbial agents, and discloses a composite microbial agent, a preparation method thereof and application of the composite microbial agent in prevention and treatment of potato scab. Wherein the microbial complex microbial inoculant is prepared from bacillus aglaia pairwise 6096, streptomyces sp. 6219 and bacillus atrophaeus 4618; the aglaia odorata pairwise bacillus 6096, the streptomyces 6219 and the bacillus atrophaeus 4618 are all preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation numbers of the aglaia odorata pairwise bacillus 6096, the streptomyces 6219 and the bacillus atrophaeus 4618 are respectively CGMCC NO.34691, CGMCC NO.34692 and CGMCC The composite microbial inoculant is good and stable in antibacterial effect, and the morbidity and disease index of potato scab can be remarkably reduced after the composite microbial inoculant is used.

The invention discloses application of an StGDPD1 gene in improvement of salt tolerance of potatoes, and belongs to the technical field of plant stress resistance. The invention aims to improve the salt tolerance of potatoes, and provides a salt-tolerant molecular breeding method for potatoes. The invention provides an application of expressing StGDPD1 protein in improvement of salt tolerance of potatoes. The amino acid sequence of the StGDPD1 protein is as shown in SEQ ID NO.2. The invention also provides an application of the StGDPD1 protein in improvement of salt tolerance of potatoes. The method is of great significance in improving the planting effect and yield of the potatoes in the salinized area.

The invention relates to the technical field of fertilizer preparation, in particular to a potato residue fermented compound fertilizer with a plant disease prevention and treatment effect and application of the potato residue fermented compound fertilizer. The invention provides a potato residue fermented compound fertilizer with the efficacy of preventing and treating plant diseases, which comprises a potato residue fermented organic fertilizer and a traditional Chinese medicine compound preparation in a mass ratio of (90-110): (11-17). The traditional Chinese medicine compound preparation and the potato residue fermented organic fertilizer are matched for use, the fertilizer efficiency of the fertilizer is further improved due to the addition of the traditional Chinese medicine compound preparation, the fertilizer has the effect of preventing and treating plant diseases, and the dual effects of promoting growth and preventing diseases are achieved.

The invention relates to a special substrate for potato virus-free breeder's seed cultivation. The special substrate comprises a substrate body and a microbial agent adsorbed on the substrate body, the matrix body comprises an organic component and an inorganic component in a volume ratio of (10-40): (90-60); the total viable count in the matrix body is not less than 2.0 * 10 < 8 > CFU / g. The microbial agent is prepared from bacillus cereus CBBL-18, bacillus paralicheniformis CBJ-7 and bacillus korla CBK-5. The invention further discloses a preparation method of the microbial agent. The substrate disclosed by the invention can efficiently promote the growth of potatoes, is disease-resistant and low in cost, and has important significance on promoting green and sustainable development of agriculture while promoting resourceful treatment of solid wastes.

The invention relates to the technical field of potato cold resistance identification, in particular to a potato seedling stage cold resistance identification device which comprises a conveying mechanism. The device further comprises an environment simulation mechanism, an identification mechanism and a culture mechanism, and the environment simulation mechanism, the identification mechanism and the culture mechanism are all installed on the conveying mechanism. According to the potato seedling identification device, a seedling raising pot bearing potato seedlings is clamped through the conveying mechanism, the potato seedlings are conveyed into the environment simulation mechanism, different low-temperature environments are simulated through the environment simulation mechanism, the potato seedlings are subjected to low temperature, and then the potato seedlings are conveyed into the identification mechanism through the conveying mechanism; the cold resistance of the potato seedlings is identified through the identification mechanism, then the potato seedlings are conveyed into the culture mechanism through the conveying mechanism, the potato seedlings are cultured for a long time in the culture mechanism, and the growth condition of the potato seedlings subjected to low temperature is observed, so that the practicability of the equipment is improved.

The present invention relates to the technical field of gene isolation and plant improvement, and provides a late blight resistance gene, a biological material, and a use. An Rpi-HMA13 nucleic acid molecule that is provided comprises: a nucleotide sequence as shown in SEQ ID NO: 1; a nucleotide sequence as shown in SEQ ID NO: 2; and a nucleotide sequence encoding the amino acid sequence as shown in SEQ ID NO: 3. The present invention provides an important gene resource for late-blight-resistant potato breeding, overcomes the problem of loss of disease resistance of potato varieties, and provides a new guarantee for lasting disease resistance of potatoes.