4363 results about "Solanum tuberosum" patented technology

Disclosed is a novel Lepidopteran- and Coleopteran-active delta-endotoxin polypeptide, and compositions comprising the polypeptide, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that comprise nucleic acid segments encoding the polypeptide. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species.

Disclosed is a novel delta -endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against colcopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneur). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

Disclosed is a novel delta-endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against coleopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneum). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

The present invention relates to the isolation and characterization of nucleotide sequences encoding novel insecticidal proteins secreted into the extracellular space from Bacillus thuringiensis and related strains. The proteins are isolated from culture supernatants of Bacillus thuringiensis and related strains and display insecticidal activity against coleopteran insects including Colorado potato beetle (Lymantria dispar) and Southern Corn Rootworm (Diabrotica undecempunctata). Insecticidal proteins encoded by nucleotide sequences that hybridize to the isolated and characterized nucleotide sequences are disclosed. Also disclosed are methods of making and using transgenic cells and plants comprising the novel nucleotide sequence of the invention.

The invention relates to a nutritional intervention composition in powder form that is taken before a meal and that extends post meal satiety. The compositions of the invention are comprised of at least one protein, C12-18 fatty acids, preferably oleic acid, all of which stimulate CCK release in the body, and a source of proteinase inhibitor which acts to prevent the deactivation of CCK. The subject compositions preferably additionally contain a source of calcium and are advantageous in that they utilize a source of proteinase inhibitor as opposed to the more costly extracts. The source of proteinase inhibitor is advantageously an extract of potato, soy, or beans containing about 10 weight percent of proteinase inhibitor. The powder compositions are mixed with a suitable liquid, preferably water, prior to ingestion.

Lipo Chitooligosaccharide (LCO) [NodBj-V(C18:1,Mefuc)] isolated from Bradyrhizobium japonicum strain 532C was able to stimulate seed germination / seedling emergence, or in the case of potato, sprouting, of a number of crop plants representing eight distantly related plant families (Poaceae, Fabaceae, Brassicaceae, Cucurbitaceac, Malvaceae, Asteraceae, Chenopodiaceae and Solanaceae) of plants, at 25 and / or at 15° C. It also promoted sprouting potato minitubers. Other LCOs [NodRM-V(C16:2,5) and LCO from R. leguminosarum] were also shown to also display growth-promoting effects on the tested crop plants. The compositions comprising at least one LCO are shown to be effective in promoting growth under both laboratory and field conditions. The invention thus also relates to methods for promoting seed germination and / or seedling emergence and / or growth of plants comprising subjecting the seeds and / or plants to an effective amount of an agricultural composition comprising at least one LCO.

The invention relates to toasted full fat, enzyme active soybean flakes, and method of making same. The inventive flakes are excellent for use in hot and cold cereal compositions including with rolled oats, in soybean flakes-potato flakes compositions for making soy protein enriched mashed potato products, in granola and power bar type compositions, in shake-and-bake type compositions, and in meat analogue veggie-burger type compositions. The inventive toasted full fat, enzyme active soybean flakes are prepared by subjecting full fat, enzyme active soybean flakes made from dehulled soybeans to relatively high heat from dry heated air for a relatively short time while moving on and / or above a moving or vibrating surface. The invention further relates to use of the inventive toasted soybean flakes in soybean flakes-potato flakes compositions, and said compositions per se.

The invention discloses potato noodles and a preparation method thereof, and relates to the field of processing of the potato noodles. According to the formula, the potato noodles provided by the invention comprise the following raw materials in parts by weight: 45-80 parts of wheat meal, 15-50 parts of whole potato flour, 1-5 parts of gluten powder and 35-45 parts of salt water, wherein the granularity of the whole potato flour is 100-180-mesh and the concentration of the salt water is 3%-9%. According to the preparation method provided by the invention, the problem that the whole potato flour is difficult to bond is further solved by adopting a secondary curing process and a gradient temperature-varying wetting drying process, and the noodles with good chewiness and mouth feel are prepared.

A planting system involving a non-damaging stimulus (3) which is placed in the vicinity of a propagule (2) causes a naturally defensive substance (5) to be produced by the propagule. This substance may exist within an encapsulant (1) for a period of time so that the propagule may have enhanced disease control until it develops sufficiently to fend for itself. In one embodiment a large encapsulant surrounds a potato tuber. This encapsulant may include chitin to cause the release of chitinase through intermediate stimulants such as mRNA and activated carbon to absorb or release particular substances. The encapsulant is bound through the use of pentosan which acts with flaked chitin to achieve an outer casing and further protect the propagule both mechanically and chemically.

A pest-combating composition including sodium lauryl sulfate and one or more of C6-12 fatty acids, preferably lauric and / or capric and / or caprylic acid, soy methyl ester, and 2-undecanone, and methods of combating pests utilizing same, are disclosed. The compositions can include a carrier oil such as silicon oil, soy methyl ester, or a vegetable oil, and can be in the form of an emulsion. The composition may be constituted as a spray composition, an aerosol, a lotion, a paste, or another compositional form. Pests that may be usefully combated with such composition include flying insects, including flies, mosquitoes, and wasps, ants, including arthropods such as fire ants, ticks, fleas, cockroaches, silver fish, thrips, gnats, aphids, Japanese beetles, and agricultural and horticultural arthropods and insects including beetles (potato and bean), flea beetles, fleahoppers, squash bugs, slugs, leaf hoppers, harlequin bugs, milk weed bugs, spiders, mites, lice, rodents, and deer.

The invention provides a method for improving the quality of expanded cut rolled stem of tobacco by microbial enzyme. The procedures are as follows:1) Preparation of bio-enzyme: (1) Aspergillus niger is activated and cultured by potato cane sugar culture medium and is then transferred into sterilized seed culture medium, and shake cultivation is carried out at the temperature of 28 DEG C for 24 hours with 250r / min<-1>; seed liquid with 10% of inoculation amount is transferred into a sterilized 500ml shake flask (containing 100ml culture liquid for enzyme), and the seed liquid carries out incubation for culturing at the temperature of 25 DEG C for 96 hours and then zymotic liquid is obtained. (2) Extraction of lignin degradation complex enzyme: After being filtered by filter paper, the zymotic liquid is centrifugally separated at the low speed of 1500rpm. The supernatant carries out fractional salting out by (NH4)2SO4 till the saturation is 0.7 to obtain crude enzyme liquid, after the crude enzyme liquid carries out ultrafiltration dialysis, the lignin degradation complex enzyme is obtained. Activities of enzyme components: 350U / L of Lip, 11U / L of MnP and 5.6U / L of Lac; 2) Improvement of the quality of expanded cut rolled stem of tobacco: The expanded cut rolled stem for cigarette is weighed and sprayed evenly by lignin degradation complex enzyme liquid containing 0.2-0.4% of expanded cut rolled stem after being diluted by distilled water at the temperature of 27 DEG C-29 DEG C. The expanded cut rolled stem of tobacco is placed in a sealed container for enzymolysis for 95-97 hours and dried naturally till the moisture content is 12.5%-15%. The result by subjective analysis suggests that the complex enzyme preparation can effectively decrease the lignin content of the expanded cut rolled stem, promote the transformation of aroma matter and improve aroma quality and taste.

The invention belongs to the technical field of potato culture, in particular to a culture technology for southern potatoes planted in autumn. The culture technology comprises the following steps of: selecting a land, preparing and ridging; applying base fertilizers; cutting potato seeds, immediately sterilizing, accelerating germination and air-drying within one hour; finishing sowing at the temperature of earth surface of 15-25DEG C at noon on the second day after seed cutting; after sowing, immediately spraying water; carrying out management on water, fertilizer and diseases, insect pests and weeds; and harvesting. According to the culture technology disclosed by the invention, the emergence rate of the potatoes planted in autumn reaches over 90 percent and the situation that the yieldof the potatoes in southern part is reduced by over 60 percent due to frost and the potatoes are not suitable for being planted in autumn is changed; meanwhile, if the potatoes are planted before andafter Mid-Autumn Festival, the potatoes can appear on the market before Spring Festival and two months earlier compared with the potatoes planted in winter; in addition, the market price of the potatoes is 0.2-0.3yuan per jin, thereby the economic benefit of the potatoes planted in autumn is increased.

The re-compounded pesticide composition includes fipronil in the weight ratio to other component of 0.02-50, and at least one of the following pesticides: lambda-cyhalothrin, fenpropathrin, cycloprothrin, esfenvalerate, etofenprox, etc. It may have functional assistant, diluent or carrier added to compound into oil emulsion, soluble liquid, super low capacity sprayed agent, water emulsion, microemulsion or other preparation forms. It has wide pesticidal spectrum, high activity, low cost, less resistance and other features, and may be applied in preventing and controlling pests of rice, vegetable, cotton, corn, fruit tree, tobacco and other crops.

The present invention relates to a method for enhancing a plant's resistance to diseases and / or bruising by transforming the plant with sense and antisense polyphenol oxidase encoding sequences from potato. The invention also relates to transgenic plants and progeny thereof with reduced polyphenol oxidase activity.

The invention discloses a method for brewing dendrobium nobile wine. Dendrobium nobile is used as one of raw materials, corns and potatoes are used as matrixes, and the dendrobium nobile wine is prepared by the procedures of pretreating, mixing, medicine feeding, primary fermenting, secondary fermenting, squeezing, clarifying, wine decocting, inspecting, bottling and storing. During production, the dendrobium nobile serving as the raw material is subjected to combined hydrolysis by using cellulose and pectinase, so that nutrients beneficial to a human body, contained in the Dendrobium nobile,can be separated out fully; and meanwhile, the corns and the potatoes are used as the matrixes, so that the production cost is reduced and simultaneously the prepared dendrobium nobile wine is red brown, has a unique scorch flavor, is mellow, palatable and slightly bitter and has a long-lasting aftertaste. A product of the dendrobium nobile wine has multiple obvious health-care functions of removing lead, enhancing memory ability and immunity, deferring senility, increasing anoxia resistance, preventing osteoporosis and the like. The method is easy to master and can be applied to scale production.

The invention provides potato-bread frozen dough and a preparation method thereof. The potato-bread frozen dough comprises the following components: potato powder and wheat flour, wherein the weight rate of the potato powder to the wheat flour is (10-40): (90-60). The preparation method comprises the following steps of: mixing the potato powder and the wheat flour, and adding water as well as an appropriate amount of yeast, sugar, salt, butter, eggs and the like into the obtained mixture so as to obtain dough; and then, after carrying out partitioning, forming and pre-fermenting on the dough, freezing the dough and then taking out the dough to carry out packing, and carrying out freezing storage on the dough. The potato-bread frozen dough (the viable count of yeasts in the frozen quality guarantee period is greater than or equal to 107 cfu / g) produced by using the method disclosed by the invention can be subjected to unfreezing, re-fermenting and baking so as to prepare fresh bread as required, and the technique facilitates the production management of bread and the freshness preservation of products; in addition, due to the addition of the potato powder, the nutritive value of bread is improved, the texture and taste of bread are improved, and the comprehensive utilization and development of potato processed by-products are facilitated, thereby having great significance in improving the additional values thereof.

The present invention discloses a portable cooked rice made of various cereals and its preparation method. Said preparation method includes the following steps: (1), washing main food material, soaking main food material in water, then taking out and draining; (2), cooking said main food material to obtain cooked rice; (3), in the process of cooking rice adding proper quantity of flavouring material, and mixing them; and (4), forming the flavoured cooked rice, packaging and sealing so as to obtain the invented portable cooked rice. The above-mentioned described main food material can be rice, millet, corn, red rice, glutinous rice, black rice, coix seed, grains of sorghum, grains of wheat, buckwheat, naked outs, soybean, black beans, mung beans, potato and sweet potato, etc. The described flavouring material includes edible salt, sugar, hot pepper and / or perfume.

The invention discloses a tambac inducer and a preparation method thereof. The tambac inducer comprises an aquilaria sinensis tree vivo stem, a PDA culture medium, a potato sucrose culture medium and an aquilaria sinensis matrix. The method comprises the following steps of cutting off a part of an aquilaria sinensis tree vivo stem, which is forming tambac, and sterilizing, culturing, separating and identifying to obtain a tambac inducible strain; activating the strain by the PDA culture medium and fermenting by the potato sucrose liquid culture medium, and then inoculating the strain to the aquilaria sinensis matrix, and culturing for 3-5 days under the conditions of constant temperature of 30 DEG C, darkness and ventilation, thus obtain the tambac inducer. The invention has simple method, can remarkably shorten the forming time of the tambac, enhance the cultivating benefit of aquilaria sinensis trees and has favorable market application prospect.

The invention provides a formula of a culture medium for industrial production of sparasis crispa and a production process. The formula of the culture medium comprises the following components in percentage by dry weight: 65 to 75 percent of pine sawdust, 10 to 20 percent of husk, 5 to 10 percent of potato powder, 5 to 10 percent of flour, 0.1 to 0.5 percent of peptone, 0.1 percent of ammonium sulfate and 1.2 to 1.8 percent of brown sugar. The production process comprises the following steps of: preparing and bagging the culture medium; sterilizing and inoculating; culturing hypha; inducing a primodium; differentiating the primodium; opening the bag; managing balls; and harvesting. The invention provides a formula of a culture medium for industrial production of sparasis crispa, which has the advantages of rich nutrition, balanced proportion, good ventilation, and capacity of meeting the requirement of industrial production. The production process has the advantages of short hypha culturing time, uniform fruiting at bag openings, good fruiting consistency, convenience of management, contribution to industrial culture, white mushroom body, high yield, and high biological transformation rate of over 43 percent.

A process and method for the production of endophytes as plant inoculant products, specifically Clonostachys rosea strain 88-710, for the promotion of plant vigor, health, growth and yield are disclosed. The endophyte, Clonostachys rosea strain 88-710 produces a fungal conidial preparation by utilizing a discrete solid substrate fermentation system, namely Potato Dextrose Agar or Malt Extract Agar. Additionally, the endophyte, Clonostachys rosea strain 88-710, can act as an inoculant to stimulate and have an additive effect with rhizobium bacteria on the production of nitrogen fixing nodules on legumes and growth enhancement e.g. beans, soybeans, peas and alfalfa. As well, Clonostachys rosea strain 88-710, can combine with rooting hormones, e.g. indole-3-butyric acid (IBA) to provide inoculant and rooting benefits to cuttings / transplants of plants.

The invention relates to a machining method of recombined puffed pigskin. Currently, a machining method for manufacturing the puffed pigskin by utilizing crushed pigskin leftover materials does not exist. The machining method comprises the following steps of: pre-treating raw materials; pickling; picking in a pickling solution for 10-12 hours, wherein the pickling solution comprises the components of water, sodium hydroxide and potassium phosphate; rinsing; carrying out secondary finishing; crushing; carrying out biological enzymolysis: adding pigskin grains, alkaline protease and saline water into an enzymolysis fermenting pot, agitating uniformly and keeping the pH (Potential of Hydrogen) value of the system to be at 7-8 at a temperature of 40-55 DEG C, and then agitating and carrying out enzymolysis at an interval for 4-6 hours; cooking and inactivating; carrying out high-speed chopping: adding pigskin protein liquid into a chopping and mixing machine when the pigskin protein liquid is hot, and then adding glutamine transaminase, aspartame, I+G, soybean isolated protein, modified starch, mashed potato and water to carry out chopping and mixing; filling and molding; freezing; slicing; and drying. According to the machining method, the crushed pigskin leftover materials can be utilized to manufacture the puffed pigskin with a comfortable mouth feel and a special flavor.

The invention belongs to the technical field of medical preparations, and in particular discloses a compound microporous starch powder hemostat and a preparation method thereof. The compound microporous starch powder hemostat uses potato starch and sweet potato starch as raw materials. The preparation method comprises the following steps: performing pregelatinization and ultrasonic pretreatment on the potato starch and the sweet potato starch, and then performing enzymatic hydrolysis on the potato starch and the sweet potato starch respectively to make pores so as to prepare microporous starches of the potato starch and the sweet potato starch respectively; in the process of enzymatic hydrolysis to prepare the microporous starches, adopting a vacuum freeze-drying method for drying the starches; using sodium trimetaphosphate to crosslink starch molecules so as to strengthen the structure; and mixing the potato crosslinking microporous starch and the sweet potato crosslinking microporous starch according to certain proportion to obtain the compound microporous starch powder hemostat. The microporous starch prepared by the method has strong hydroscopic property and obviously improved water-absorbing speed; besides, compared with the prior single-starch polysaccharide hemostatic material, the ingredients of the compound have improved performance and good hemostatic effect.

The invention belongs to the field of potato stock seed culture and particularly relates to a method for culturing potato virus-free test-tube plantlets in a water culture mode. The method comprises the steps of seedling exercising, rooting treatment, nutrition liquid preparation, field planting, culture and transplanting. In aspects of variety, culture density, nutrition liquid concentration and rooting agent concentration, the propagating factor and the strong seedling quantity are increased, corresponding measures are adopted for handling in each link, the propagating factor of virus-free seedlings is improved, healthy and strong virus-free seedlings are obtained, and the soilless culture mode requirements of fog culture, substrates and the like are met, so the healthy and efficient potato stock seed production is reached. The original virus-free, germless and insect-free characteristics of the plantlets are maintained, the characteristics of simple technology, low cost, strong germchits and the like are also realized, in addition, the survival rate of the water culture potato seedlings transplanted to the substrates or other soilless culture (such as fog culture) is very high and can sometimes reach 99 percent, and the production efficiency of potato stock seeds is improved.

The embodiment of the invention provides a high-yield cultivation method for north potatoes through drip irrigation under mulch, and relates to the field of potato cultivation. The water utilization rate and the fertilizer utilization rate can be improved, and the yield of the potatoes can be increased in a multiplied mode. The method comprises the steps of soil selection and preparation, drip irrigation system installation, variety selection and potato seed processing, sowing, irrigation, top application, fertilization management and field management. According to the irrigation standard, the field capacity in the germination period ranges from 60% to 65%, the field capacity in the seedling period ranges from 65% to 70%, the field capacity in the tuber forming period ranges from 75% to 80%, the field capacity in the tuber expanding period ranges from 75% to 80%, the field capacity in the starch accumulating period ranges from 60% to 70%, the irrigation norm of the total growth period of the potatoes cultivated through drip irrigation under the mulch ranges from 80 m<3> / 667 m<2> to 120 m<3> / 667 m<2>, the dissolubility fertilizer is selected during the top application, a differential pressure type fertilization tank is adopted, and dressing of 15 kg / 667 <2> of urea during irrigation is carried out.

The invention provides an aeroponic virus-free seed potato breeding method and equipment. The breeding method includes the following steps: equipment cleaning and disinfection, greenhouse disinfection through a smoke drug, seedling hardening, planting and atomization, illumination and temperature management, seedling shedding, overgrowth and lodging control, nutrient solution concentration management, nutrient solution pH value management, nutrient solution temperature management, pest and disease damage prevention, and harvesting. The breeding equipment includes cultivation tanks, cultivation plates, a nutrient solution, a solution storage container, a solution delivery pipe, atomization nozzles, a circulating pump, and a nutrient solution return pipe, wherein each cultivation tank is a cuboid tank of which the length is much larger than the width, the inner surface of each cultivation tank is painted black, the outer surface of each cultivation tank is painted silvery grey, and each cultivation tank is supported from the ground through a bracket; the solution storage container is an underground solution storage pool, a temperature sensor is arranged in the solution storage pool, and a nutrient solution cooling thermolator is arranged outside the solution storage pool. The method provided by the invention can reduce the seedling time, prolong the collection period, and increase the yield; the number of stolons is increased, so as to lay a foundation for a higher yield of virus-free seed potatoes. The equipment provided by the invention is convenient to manage, can save energy, and runs reliably.

The invention discloses a trichoderma spore powder as well as a preparation method and application thereof. The trichoderma spore powder contains trichoderma spore powder and a drying aid, wherein the trichoderma spore powder is the spore powder of Trichoderma sperellum T1 which is preserved in the general microorganism center of CCCCM (China Committee for Culture Collection of Microorganisms) and has the preservation number of CGMCC NO: 3531 and a rDNA ITS region segment sequence shown in SEQ ID NO:1; the drying aid is one or more of beta-cyclodextrin, potato starch and soluble starch. The trichoderma spore powder has an effective prevention and control effect on banana vasicular wilt, and has a remarkable antagonism effect on various pathogenic funguses, especially on soil-borne funguses. The preparation time is saved with the preparation method of the trichoderma spore powder, which can be used for preparing the trichoderma spore powder which has high density, high germination rateand high drying efficiency and is easy to store, transport and use.

The invention relates to a cultivating method of healthcare cassia seed contained coprinus comatus. The cultivating medium is prepared by components: by weight, 50-60 parts of cornstalk, 30-40 parts of cottonseed hull, 5-10 parts of rice bran, 10-15 parts of potato powder, 1-2 parts of land plaster, 1-2 parts bagasse, 0.2-0.3 part of calcium carbonate, 2-3 parts of quick lime, 1-2 parts of sealwort powder, 1-3 parts of immature bitter orange powder, 1-2 parts of radix angelicae powder, 1-2 parts of cassia seed powder, 1-3 parts of caragana microphylia flower, 1-3 parts of momordica grosenori leaves, 2-3 parts of sepiolite powder, and 8-10 parts of bone soup. The cultivating method includes mixing the components according to proportion, adding water to mix, bagging, sterilizing, inoculating, growing fungus in a growing room, replenishing nutrient solution and fruiting to obtain coprinus comatus. The coprinus comatus cultivated is rich in nutrients, high in content of microelements, vitamins and calcium, has healthcare function of the cassia seeds, and is high in anti-infection ability and yield during fruiting of the coprinus comatus.