646 results about "Solanum tuberosum" patented technology

The invention discloses a nutrient solution concentration regulation and control method and system for potato aeroponic cultivation, and relates to the technical field of fertilization, and the method comprises the following steps: extracting root feature data and concentration feature data; establishing a concentration trend prediction model based on the root feature data and the concentration feature data, and predicting the concentration change trend of the nutrient solution under different conditions by using the concentration trend prediction model; analyzing the change trend of the nutrient solution concentration under different conditions by using a feature analysis algorithm, and identifying key factors influencing the stability of the nutrient solution concentration; and based on the key factors, the nutrient solution is dynamically prepared. Key factors influencing the concentration stability are identified through a feature analysis algorithm, and the nutrient solution ratio is dynamically regulated and controlled, so that intelligent management and accurate supply of the nutrient solution concentration are realized, and effective support is provided for nutrition optimization of the potato aeroponic culture whole period.

The invention discloses talaromyces purpurogenum as well as a fermented supernatant and application thereof. The preservation number of the talaromyces purpureogenus F-4 is CCTCC NO: M 20252023, and the talaromyces purpureogenus F-4 has the growth promoting effects of dissolving phosphorus, fixing nitrogen and generating siderophores, also has the disease preventing and treating effects of preventing and treating garlic root rot, potato scab and wheat stem rot, and also can resist salt stress, alkali stress, salt-alkali composite stress, heavy metal stress and osmotic pressure stress. The fermentation supernatant of the talaromyces purpureus F-4 can be used for inhibiting the growth of fusarium solani spores, streptomyces botrytis spores and fusarium pseudograminearum and the germination of the spores of the fusarium solani, the streptomyces botrytis and the fusarium pseudograminearum. The talaromyces purpurogenus and the fermented supernatant thereof can effectively control the growth and reproduction of plant pathogens, reduce the risk of disease occurrence, reduce the dependence on chemical pesticides, and reduce the risk of environmental pollution and agricultural product residues, and have important significance for promoting the sustainable development of agriculture.

The invention discloses protease for improving the resistance of potatoes to late blight as well as a coding gene and application thereof, and belongs to the technical field of genetic engineering and plant disease resistance breeding. The amino acid sequence of the protease StRD21 is as shown in SEQ ID NO.2, or the protease StRD21 has a derivative sequence with the same disease-resistant function. The nucleotide sequence of the coding gene is as shown in SEQ ID NO. 1. The invention also provides a specific primer pair for cloning the gene, a potato recombinant overexpression vector containing the gene and a transgenic host cell. The StRD21 gene is introduced into potatoes and overexpressed, so that the resistance of the potatoes to late blight can be remarkably enhanced. The invention provides an effective gene resource and a biotechnological means for cultivating a new variety of disease-resistant potatoes.

The invention discloses a potato cold resistance identification method and system, and relates to the technical field of cold resistance identification. The method comprises the following steps: cultivating potato tissue culture seedlings in a standardized manner, and randomly dividing the seedlings into a low-temperature stress group and a normal-temperature control group; performing standardized low-temperature treatment including slow descent and constant-temperature stages on the stress group; the method comprises the following steps: acquiring multi-source data such as chlorophyll fluorescence parameters, canopy thermal imaging, digital image phenotypes and biochemical indexes, and forming a unified data set after derivative index calculation and standardized fusion; inputting the fusion data into an improved neural network model, training the model through a particle swarm optimization algorithm, and outputting a quantitative potato cold resistance index; automatically evaluating the cold resistance grade according to the potato cold resistance index and generating an identification report; standardization of the identification process, fusion analysis of multi-dimensional indexes and intelligent result analysis are achieved, and the accuracy, efficiency and practicability of potato cold resistance identification are remarkably improved.

The application discloses an NLR plug-in gene for improving plant disease resistance and application thereof, and belongs to the field of plant molecular biology and biotechnology.The NLR plug-in gene StEM1 has a whole gene sequence as shown in SEQ ID NO.1 and a CDS sequence as shown in SEQ ID NO.2, and the amino acid sequence of the encoded protein is as shown in SEQ ID NO.3.The gene plays a key role in improving plant disease resistance, especially resistance to field late blight variant bacteria.In the NLR background, overexpression of the gene significantly promotes the disease resistance of tobacco and potato to pathogenic pythium, and the gene is a gene for enhancing plant disease resistance by improving ETI immunity.Overexpression of the gene in the NLR background is obtained by a genetic transformation method, and a strain line is obtained to resist field variant strains, so as to cope with field diseases of crops.The application provides an excellent gene resource for green prevention and control of late blight and disease-resistant genetic engineering breeding.

The invention discloses a potato seedling grading environment parameter dynamic optimization regulation and control method, and relates to the technical field of agricultural informationization. Based on an Internet of Things sensor deployed in a target area, stem diameter, chlorophyll content and plant height data of potato seedlings are collected, the seedlings are divided into weak seedlings, middle seedlings and strong seedlings by adopting K-means clustering, and the weak seedlings, the middle seedlings and the strong seedlings are classified into a weak seedling classification model, a middle seedling classification model and a strong seedling classification model; setting the duration of the initial light period; and carrying out gradient photoperiod test on each type of potato seedlings by taking the stem elongation rate and the chlorophyll content as constraint conditions. Through grading photoperiod modeling and ant colony algorithm dynamic optimization, the limitation of traditional fixed photoperiod regulation and control is broken through, the initial photoperiod range is set based on the seedling physiological difference, and the photoperiod-growth rate response curved surface model is constructed in combination with the stem elongation rate and chlorophyll content constraint conditions. The influence of different photoperiod combinations on seedling growth is quantified, accurate matching of photoperiods and seedling requirements is ensured, excessive growth or premature senility is avoided, and the growth rhythm stability and the resource utilization efficiency are improved.

The invention belongs to the technical field of agricultural biology, and particularly relates to a transcription factor StBSB with a regulation effect on steroid glycoalkaloid accumulation of potatoes and application of the transcription factor StBSB, the transcription factor StBSB is encoded by a potato gene Soltu.DM.05G001620, and the nucleotide sequence of the potato gene Soltu.DM.05G001620 is shown as SEQ ID NO.1. The invention further discloses a preparation method of the transcription factor StBSB. According to the application, a new insight is provided for understanding a transcriptional regulation mechanism of SGAs in solanaceae plants, and a key theoretical basis and genetic resources are provided for directionally improving the content of anti-nutritional compounds through genetic means.

The invention belongs to the technical field of gene engineering, and relates to application of a potato late blight bacterium response gene StRTP5 in regulation and control of potato disease resistance. The invention provides application of a potato late blight response gene StRTP5 in regulation and control of potato disease resistance, the potato late blight response gene StRTP5 comprises a gene StRTP5a and a gene StRTP5b, the gene StRTP5a and the gene StRTP5b negatively regulate and control resistance of potatoes to phytophthora infestans and alternaria solani, and the gene StRTP5a and the gene StRTP5b have potential to be used for creating potato broad-spectrum disease-resistant materials.

The invention relates to the field of molecular cytogenetics and potato ploidy breeding, in particular to a potato ploidy identification method for marking and counting chromosome centromere based on a potato CENH3 antibody and a ploidy detection kit. According to the method, the chromosome centromere is marked by specifically recognizing the antibody of the potato centromere nucleosome core histone CENH3 and combining the immunofluorescence technology, so that the accurate counting of the chromosome centromere is realized at the single cell level, and the ploidy of potatoes is judged. The method comprises the following steps: preparing a leaf cell suspension, sequentially incubating a potato CENH3 antibody and a labeled secondary antibody after immobilization, and observing and counting by a microscope after redyeing. The method has the advantages of high species specificity, high throughput and low cost, is not limited by special material taking parts of root tips or shoot tips and cell division periods, can accurately identify the uploid and aneuploid of potatoes, and provides an efficient tool for potato germplasm resource evaluation and cross breeding.

The invention discloses a monitoring analysis system and method for potato breeding, and relates to the technical field of agricultural information technology and intelligent breeding, and the system comprises a multi-modal data acquisition module which is configured to synchronously obtain greenhouse environment parameters, potato plant multispectral imaging data and leaf metabolite concentration data; and the space-time causal modeling module is connected to the multi-modal data acquisition module, and is used for constructing a dynamic correlation model among environmental fluctuation, gene expression and phenotypic characteristics, and deducing and quantifying the genetic effect of the environmental abnormal event based on anti-fact intervention. According to the monitoring analysis system and method for potato breeding, through multi-modal data fusion and space-time causal modeling, the accuracy and early warning timeliness of potato breeding monitoring are improved, the limitation of traditional visible light imaging is broken through, and the incubation period disease recognition accuracy is improved compared with a traditional method.

The invention discloses an arabidopsis thaliana RK3 gene and application of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene in improvement of plant disease resistance, and belongs to the technical field of plant genetic engineering. According to the application of the arabidopsis thaliana RK3 gene to improvement of plant disease resistance, the nucleotide sequence of the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.1, the amino acid sequence of S structural domain receptor kinase RK3 coded by the arabidopsis thaliana RK3 gene is shown as SEQ ID NO.2, and further plants are selected from arabidopsis thaliana, oilseed rape, rice, tomatoes, potatoes, peanuts, soybeans, cotton, tobacco, cucumbers, watermelons and the like. The expression of the plant disease resistance comprises infection resistance to wild-type pseudomonas syringae, effector engineering strains thereof and botrytis cinerea. It is clear that the arabidopsis thaliana RK3 gene and the encoded protein thereof can remarkably enhance the infection resistance of plants to various pathogenic bacteria for the first time, disease prevention and control are achieved by activating the autoimmune system of the plants, and an effective path is provided for reducing chemical pesticide application and promoting agricultural green development.

The invention relates to a potato leaf disease area positioning method, system and device.The method is applied to the potato leaf disease area positioning device.The method comprises the steps that a target infrared image of a potato leaf is segmented into a normal area and an abnormal area according to the environment temperature through a disease segmentation model obtained in advance; determining the water content of the target infrared image according to the leaf spectrum information through a pre-obtained water content prediction model; the leaf emissivity characteristic is calculated according to the water content through a pre-obtained temperature correction model, the temperature of the abnormal area is corrected based on the emissivity characteristic and the water content characteristic, and the temperature correction model is obtained based on potato leaf characteristic training; and through a pre-trained disease identification model, identifying a disease area from the abnormal area after temperature correction.

Disclosed are a Solanum tuberosum drought tolerance-related transcription factor gene StPIF4 and the use thereof. A CDS sequence of StPIF4 has a length of 1554 bp, has a nucleotide sequence shown as SEQ ID No. 1, and encodes 517 amino acids, the amino acid sequence being shown as SEQ ID No. 2. The gene is located in a cell nucleus. The gene reduces the water loss of Solanum tuberosum seedlings under the drought stress by adjusting the opening degree of stomas, thus enhancing the drought tolerance of Solanum tuberosum. The present invention provides a new genetic material and a theoretical basis for analyzing the drought-tolerant molecular mechanism and drought-tolerant breeding improvement of Solanum tuberosum.

The invention discloses dsRNA of a targeted potato beetle heat shock protein HSP70-KDa3 gene as well as a preparation method and application of the dsRNA of the targeted potato beetle heat shock protein HSP70-KDa3 gene. In order to solve the problems that an existing RNAi target gene is prone to off-target, poor in field stability and the like, the potato beetle heat shock protein gene LdHSP70-KDa3 serves as an RNA interference target for the first time, and a double-stranded RNA molecule shown as SEQ ID NO: 1 and a preparation method for preparing the double-stranded RNA are provided. Feeding experiments prove that the double-stranded RNA can efficiently silence target genes, the survival rate of potato beetle larvae is remarkably reduced, and feeding and growth development of the potato beetle larvae are inhibited. In addition, the invention further provides a biological preparation containing the double-stranded RNA and application of the biological preparation in prevention and treatment of potato beetles. The dsRNA targeting the HSP70-KDa3 gene provided by the invention has the characteristics of high efficiency, specificity, environmental friendliness and the like, and a new technical scheme is provided for green prevention and control of potato beetles.

The invention belongs to the technical field of gene engineering, and relates to application of glutaredoxin StGRX5 in plant disease resistance. The invention provides an application of glutaredoxin StGRX5 in the disease resistance of plants. The glutaredoxin StGRX5 is silenced in potato plants, and the resistance of the potato plants to phytophthora infestans can be enhanced.

The invention discloses a plant immunomodulatory gene RDP as well as an expression vector and application thereof. The inventor finds a plant immunomodulatory factor gene RDP from potatoes, after a corresponding sequence is obtained through cloning, agrobacterium competent cells are transferred into nicotiana benthamiana, it is verified that the plant immunomodulatory factor gene RDP can be successfully expressed in other plants, and it is verified that the pathogenic effects of various phytophthora are good. The gene is proved to be capable of effectively improving the disease resistance of the nicotiana benthamiana after being expressed. Through objective evaluation of an agrobacterium tumefaciens-mediated transient expression system, it is found that the plant immunomodulatory factor RDP can induce plants to generate resistance, the gene can be directly introduced into the plants through a transgenic technology due to the powerful function of the gene, disease-resistant varieties are developed and cultivated, and the gene has great significance in prevention and treatment of plant diseases.

The invention provides an application of potato laccase StLAC19 in a shade avoidance reaction. The overexpression of the StLAC19 gene can significantly enhance the shading tolerance of potato plants, and effectively improve the lignin content, the osmotic regulation substance content, the chlorophyll content, the stalk strength, the CAT and POD enzyme activity and the PRO and MDA content; on the contrary, interference on expression of the StLAC19 gene results in a contrary result. The result shows that the StLAC19 gene positively regulates and controls the shading reaction of potato plants. The screening of varieties with high expression of the StLAC19 gene is helpful for obtaining materials with stronger shade-avoiding tolerance, so that the gene can be used as a target gene for breeding shade-avoiding tolerance type potato varieties.

The invention discloses application of an StGDPD1 gene in improvement of salt tolerance of potatoes, and belongs to the technical field of plant stress resistance. The invention aims to improve the salt tolerance of potatoes, and provides a salt-tolerant molecular breeding method for potatoes. The invention provides an application of expressing StGDPD1 protein in improvement of salt tolerance of potatoes. The amino acid sequence of the StGDPD1 protein is as shown in SEQ ID NO.2. The invention also provides an application of the StGDPD1 protein in improvement of salt tolerance of potatoes. The method is of great significance in improving the planting effect and yield of the potatoes in the salinized area.

The present invention discloses an integrated intelligent potato seed potato cutting and selection machine, which relates to the technical field of potato seed selection and cutting machines, and comprises a feeding device, an arranging device, a cutting device, a sorting and positioning device, a visual recognition device, and a seed selection device, which are linearly connected in sequence; the feeding device conveys a group of potato seed potatoes in a panel to the arranging device; the arranging device arranges the group of potato seed potatoes and makes them enter the cutting device for cutting, and the cutting device is installed at the end of the arranging device; the sorting and positioning device makes the cut seed potatoes enter the visual recognition device; the visual device is installed on the sorting and positioning device through a bracket, and can collect eye information of the cut potatoes and convey the identification information to the seed selection device; the seed selection device can realize the kicking out of unqualified cut potatoes; the present invention realizes the integration of feeding, arranging, cutting, sorting and positioning, and air-blowing seed selection, reduces labor costs, and improves the efficiency and quality of seed potato cutting.

The present invention relates to the technical field of microbial agents, and discloses a microbial composite agent, a preparation method thereof, and its use in preventing and treating potato scab. The microbial composite agent comprises: P. milanoxanus 6096, Streptomyces 6219, and Bacillus atrophaeus 4618; P. milanoxanus 6096, Streptomyces 6219, and Bacillus atrophaeus 4618 are all deposited in the General Microbiology Center of the China National Center for Microbiological Culture Collection, with deposit numbers CGMCC No. 34691, CGMCC No. 34692, and CGMCC No. 34952, respectively. The microbial composite agent of the present invention has a good and stable antibacterial effect and can significantly reduce the incidence and disease index of potato scab after use.

The invention discloses an endophytic subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. Subsp. The strain can inhibit the growth of different physiological races of phytophthora infestans, and has inhibitory activity on various pathogenic oomycetes. The fermentation liquor of the strain contains micromolecular antibacterial substances with relatively high stability, and has remarkable antibacterial activity; protein components in fermentation liquor can induce plants to generate resistance, and infection of phytophthora infestans is effectively inhibited. Different types of microbial agents prepared by utilizing the strain DS1 can be used for effectively preventing and treating potato late blight, and the microbial preparation is used for seed dressing treatment of potatoes and wheat and can be used for remarkably promoting the growth of crops.

The present invention relates to diploid, fertile, self-compatible and essentially homozygous potato lines, wherein said lines comprise an agronomically desirable trait such as vigour. The invention further relates to methods for producing such plants and to hybrid seeds obtained by crossing such homozygous potato lines and to potato plants grown from said seed.

The invention provides a botanical fungicide of a chaulmoogra seed extract, which takes sub-chaulmoogra seed oleic acid as a marker, and application of the botanical fungicide in preparation of medicines for preventing and treating plant diseases caused by pathogenic fungi and oomycetes. The hydnocarpus anthelmintica extract is obtained by extracting hydnocarpus anthelmintica seeds with a plurality of different types of organic solvents such as ethanol, petroleum ether and the like and treating the seeds by a physical method, and the hydnocarpus anthelmintica extract contains sub-hydnocarpus anthelmintica oleic acid as markers in different proportions. The extract has the effects of inhibiting conidium germination, bud tube growth or appressorium formation of fungi and oomycetes, effectively preventing pathogenic bacteria from infecting host plants and preventing and treating important plant diseases such as rice blast, mango anthracnose, potato late blight and rice false smut.

The present invention relates to diploid, fertile, self-compatible and essentially homozygous potato lines, wherein said lines comprise an agronomically desirable trait such as vigour. The invention further relates to methods for producing such plants and to hybrid seeds obtained by crossing such homozygous potato lines and to potato plants grown from said see.

The invention belongs to the technical field of intelligent navigation of agricultural machinery, and particularly relates to an autonomous navigation system of field unmanned transportation equipment after potatoes (such as potatoes and sweet potatoes) are harvested, in particular to a potato operation line identification method based on binocular camera multi-modal information dynamic weighting. Aiming at a complex field environment (soil is loosened and turned over, scattered potato blocks / weeds are mixed, and an original ridge-shaped structure is locally damaged) after the operation of a harvester, a ridge line track required by the driving of a transport vehicle is difficult to stably identify in a scene of strong light overexposure, weak light noisy points and shadow alternation by a traditional visual method. According to the method, an IntelRealSenseD456 active stereoscopic vision binocular camera is carried, RGB images and depth information are fused in real time, and multi-modal data are dynamically weighted based on illumination intensity, so that the robust perception capability of the unmanned transport vehicle on the geometric boundary of the potato ridge is improved, the vehicle is ensured to accurately run along a harvested field ridge operation line, and the situation that the potato blocks are rolled and scattered or deviated from a path is avoided.

This invention relates to the field of microbial inoculants, and discloses a multi-strain microbial preparation for the prevention and control of potato scab, its preparation method, and its application. The multi-strain microbial preparation includes: *Bacillus atrophicus* 6052, *Bacillus compostii* 6069, *Microbacterium natriureticum* 6035, and *Bacillus atrophicus* 4618; all of these strains are deposited at the China General Microbiological Culture Collection Center (CGMCC), with accession numbers CGMCC NO.34693, CGMCC NO.34694, CGMCC NO.34695, and CGMCC NO.34952, respectively. The multi-strain microbial preparation of this invention exhibits good and stable antibacterial effects, and its use can significantly reduce the incidence and disease index of potato scab.

The invention belongs to the technical field of microcapsule preparation and probiotic embedding, and particularly relates to a potato resistant starch-based lactobacillus plantarum microcapsule as well as a preparation method and application thereof. The preparation method comprises the following steps: S1, preparing the potato type II resistant starch; s2, preparing a bacterial suspension; and S3, preparing the lactobacillus plantarum embedded microcapsule. The potato starch is subjected to enzymolysis and drying treatment to obtain the potato type II resistant starch, and digestible starch components can be specifically eliminated; then, potato type II resistant starch, sodium alginate, Tween 80, soybean oil and calcium chloride are used for forming an embedding system for embedding lactobacillus plantarum, a compact and stable microcapsule network structure is constructed, the microcapsule network structure is good in stability and not prone to digestion, absorption and decomposition, the ionic crosslinking-hydrogen bond synergistic interaction is achieved, and the lactobacillus plantarum microcapsule is prepared. The embedding effect and the survival rate of the lactobacillus plantarum are improved.

The invention discloses a strain of streptomyces rochei PBSH7 and an application of the strain of streptomyces rochei PBSH7. Belongs to the technical field of plant disease biological control. The strain for preventing and treating the potato scab, provided by the invention, is a streptomyces rochei strain PBSH7, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) No.15827. When the streptomyces rochei PBSH7 is used for furrow application treatment, the control effect on the potato scab reaches 78.37%, and the growth and tuber quality of potatoes can be promoted. The compound tetremycin C is obtained by carrying out enlarged fermentation, extraction, vacuum concentration and separation and purification by a reversed-phase preparative high performance liquid chromatograph on strains, and the bacteriostatic effect of the compound tetremycin C on potato scab is 125 mu g / mL, and the bacteriostatic diameter of the compound is 22.33 mm. The streptomyces rochei PBSH7 provided by the invention is used for preventing and treating potato scab, and has good development and application prospects.

The invention discloses application of a potato transcription factor StBBM gene in potato tuberization breeding, and aims to regulate formation and development of potato tubers through silencing and overexpression of the StBBM gene so as to improve the yield and quality of the potato tubers. Through overexpression of the StBBM gene, the tuberization efficiency of the potatoes is remarkably improved, the tuberization time is advanced, early maturing is promoted, and the planting period is shortened. In addition, the tuber shape for silencing the gene is changed from an ovulate shape to a long cylinder shape, and the weight of a single potato plant is reduced, so that the storage and transportation efficiency can be improved, the market preference can be met, more choices are provided for breeding, and the cultivation of potato varieties adapting to different conditions is facilitated. Generally, the present invention enhances the production and adaptability of potatoes and brings advantages to the entire production chain of potatoes by improving the form and weight of tubers.

The invention discloses an irrigation system based on potato canopy temperature collection and a water stress index, and relates to the field of potato planting, the system comprises a temperature collection module used for collecting the canopy temperature of potatoes in real time; the meteorological data acquisition module is used for acquiring the wind speed, the air temperature and the relative humidity of the target farmland; the central processing module is used for performing compensation processing on the canopy temperature based on the wind speed to obtain a compensated canopy temperature; calculating a water stress index based on the compensation canopy temperature and the air temperature; the decision module is used for generating an irrigation decision signal based on the water stress index and the relative humidity; and the execution module is used for controlling and executing irrigation operation based on the irrigation decision signal. By collecting the temperature of the potato canopy and combining a wind speed compensation mechanism to eliminate environmental interference, the problem that traditional soil humidity detection is lagged is solved, and irrigation response timeliness is remarkably improved.