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60 results about "Expressivity" patented technology
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Soft-sending chat messages
ActiveUS20130047099A1Facilitate communicationIndoor gamesNatural language data processingExpressivityMultimedia
Owner:DISNEY ENTERPRISES INC
System and Method for Generating Phrases
ActiveUS20120191445A1Increase the number ofNatural language translationSemantic analysisExpressivityVirtual world
Techniques are disclosed for generating phrases for selection by users communicating in an online virtual world environment. A phrase generation engine automatically constructs new phrases using pre-approved words from a dictionary of frequently used words and language-specific rules for phrase formation to ensure safety and supplement an existing database of commonly used, pre-approved phrases. Increasing the number of phrases that are available for selection by a user increases the user expressivity. Each word in the dictionary is annotated with semantic and grammatical information that constrains how the word is combined with other words to generate a new phrase. Each new phrase may also be tagged to enable translation into a different language so a phrase in a first language selected by a first user may be displayed in a second language to a second user.
Owner:DISNEY ENTERPRISES INC
Enzyme synthesized by Chinese caterpillar fungus hirsutella sinensis to metabolize guanine nucleotide and genes and application of enzyme
InactiveCN102690796AIncrease productionEnhance expressive abilityFungiBacteriaBiotechnologyBiosynthetic genes
The invention relates to IMP (inosine monophosphate) dehydrogenase synthesized by 'Bailing' producing fungus Chinese caterpillar fungus hirsutella sinensis to metabolize guanine nucleotide based on xanthosine monophosphate and coding genes and application of the IMP dehydrogenase. The IMP dehydrogenase comprises proteins shown by SEQ ID No.1, SEQ ID No.2 and SEQ ID No.3, and coding genes of the IMP dehydrogenase correspond to nucleotide sequences shown by SEQ ID No.4, SEQ ID No.5 and SEQ ID No.6. Detailed studies on metabolic pathways of the xanthosine monophosphate synthesizing the guanine nucleotide in principle are carried out and include that cloned DNAs (deoxyribonucleic acids) in the provided nucleotide sequences can be transferred into engineering bacteria by means of transduction, transformation and transconjunction, and high expressivity is given to the host guanine nucleotide by adjusting expressions of guanine nucleotide biosynthetic genes, so that an effective way for increasing output of the guanine nucleotide is provided, and the IMP dehydrogenase and the coding genes have significant application prospects.
Owner:ZHEJIANG UNIV OF TECH +1
Nano antibody and preparation method thereof
ActiveCN109897110AGood water solubilityImprove patienceMetabolism disorderAntibody ingredientsAntigenSolubility
The invention provides a nano antibody. The nano antibody comprises the characteristics that (1) an amino acid sequence shown in SEQ ID NO:1 is achieved; or (2) compared with the (1), the at least 70%identity, the at least 75% identity, the at least 80% identity, the at least 85% identity, the at least 90% identity, the at least 95% identity, and the at least 99% identity are achieved. The nano antibody is an antibody specifically targeting PCSK9, the native conformation PCSK9 can be combined, and the nano antibody has the high water solubility, high tolerance, high stability, high antigen bondability, low immunogenicity, high tissue penetrating power, and high expressivity.
Owner:SHENZHEN HUADA GENE INST
Constant tension center coiling control system
ActiveCN101423153ASolve control scheme problemsEasy to operateWebs handlingProduction lineExpressivity
The invention relates to a rubber rolling apparatus, in particular to a constant-tension central rolling control system relating to a central rolling roller and a motor. A controller is arranged between the motor and the rolling roller; a lowest rolling diameter is set in the controller, then the motor is started and the speed of the motor is fed back to the controller through a DC speed regulator and the controller simultaneously receives the product-line speed of the rolling roller, and after the calculation of a software, the rolling diameter value is output to the DC speed regulator. The invention is mainly applied to the constant-tension central rolling of rubber industry and also can be widely applied to the constant-tension central rolling of other industries. The maneuverability and expressivity are higher and the maintenance is convenient; the running is stable and reliable; and the invention is most characterized in that the depending on the control card of the constant-tension central rolling is broken through and the control arithmetic is unique so as to lead the control system to get higher control precision and reduce the manufacturing cost.
Owner:大连橡胶塑料机械有限公司
DMD-based imaging pre-processing device and method
InactiveCN103716538AImprove dynamic rangeRealize the processing functionTelevision system detailsColor television detailsImaging qualityVisual perception
The invention discloses a DMD-based imaging pre-processing device and a DMD-based pre-imaging processing method. The DMD-based pre-imaging processing device comprises a CCD, a first lens group, a DMD, a second lens group, a DSP and an object space working surface. Spatial one-to-one correspondence between micro mirror-image elements in the DMD and CCD pixels is realized, and coordinate corresponding relation imaging of the CCD and the DMD and corresponding relation imaging of the DMD and the object space working surface are performed, and therefore, the pre-imaging processing device can process incident light before imaging. According to the DMD-based imaging pre-processing device and method of the invention, the focus of the work of image processing is shifted from post-imaging to an optical path of pre-imaging; and corresponding imaging of the CCD and the DMD is utilized, such that the gray range index of an imaging gray histogram can be increased, and therefore, the dynamic range of an imaging system can be expanded. With the DMD-based imaging pre-processing device and method of invention adopted, defects in environmental lighting and a field of view can be eliminated, and imaging quality and visual expressivity of an object can be improved. The DMD-based imaging pre-processing device and method of the invention can be widely applied in fields such as industrial inspection, medical imaging and astronomical observation.
Owner:TIANJIN UNIV
Nano antibody and preparation method thereof
ActiveCN109897107AGood water solubilityImprove patienceDigestive systemImmunoglobulins against cell receptors/antigens/surface-determinantsSolubilityExpressivity
The invention provides a nano antibody. As for the nano antibody, (1) an amino acid sequence shown in SEQ ID NO:1 is achieved; or (2) compared with the (1), the at least 70% identity, the at least 75%identity, the at least 80% identity, the at least 85% identity, the at least 90% identity, the at least 95% identity, and the at least 99% identity are achieved. According to the embodiments, the nano antibody is an antibody specifically targeting an ion channel TRPV3, the native conformation TRPV3 can be combined, and the nano antibody has the high water solubility, high tolerance, high stability, high antigen bondability, low immunogenicity, high tissue penetrating power, and high expressivity.
Owner:SHENZHEN HUADA GENE INST
Coordination-model-based method for obtaining video abstract
ActiveCN106034264AImprove accuracyMeasuring expressivitySelective content distributionSpecial data processing applicationsDictionary learningDispersity
The invention, which belongs to the video processing technology, particularly relates to a coordination-model-based method for obtaining a video abstract. The method comprises: (1), generating a feature expression matrix of a video frame; (2), evaluating significance of the video frame comprehensively by a coordination mode; and (3), extracting a key frame to form a video abstract. According to the invention, with construction of the coordination model, expressivity and dispersity of the video frame are considered, so that accuracy of the video abstract is improved. Constraint information of the geometric structure of the video frame is added into the dictionary learning process, so that sparse representation becomes accurate and expressivity of the video frame can be measured accurately. With a dispersity measuring standard based on similarity measurement, redundant frame extraction can be avoided, thereby improving conciseness of the video abstract.
Owner:XI'AN INST OF OPTICS & FINE MECHANICS - CHINESE ACAD OF SCI
Artificial synthetic porcine interferon and method for preparing porcine interferon
The invention relates to a synthetic porcine interferon gene, a carrier-host cell containing same and a method for preparing porcine interferon. The synthetic porcine interferon gene has an SEQ ID NO.1 nucleotide sequence shown in a figure 1. The preparation method comprises the steps of synthesizing a synthetic porcine alpha-IFN gene and the carrier-host cell containing the gene, recombining pichia for fermentation to produce the porcine interferon and purifying the obtained porcine alpha interferon. An expression system has the characteristics of high expressivity, high stability and high secretion. The pichia as host bacteria is small in the secretion of own protein, large in the secretion of high-activity foreign protein and beneficial to downstream separation and purification, is a eukaryotic expression system suitable for expressing foreign gene, and is beneficial to large-scale batch production.
Owner:CHENGDU QIANKUN VETERINARY PHARMA
Organic electroluminescent device and display unit
InactiveUS6916552B2High color purityImprove expressivenessDischarge tube luminescnet screensLamp detailsDisplay deviceHole transport layer
An organic electroluminescent device capable of blue emission with a high color purity, and a display unit capable of full-color display with high color expressivity by using the organic electroluminescent device are provided. The organic electroluminescent device includes at least a hole transportation layer and a luminescent layer held between a lower electrode to become an anode and an upper electrode to become a cathode in a state of lamination in that order from the anode side. The luminescent layer is made of a spiro compound, and the hole transportation layer is made of triphenylamine tetramer. Furthermore, the display unit includes organic electroluminescent devices as blue-emitting elements arrayed for a plurality of pixels.
Owner:SONY CORP
Enzyme for synthesizing cetyl-coenzyme A through cordyceps sinensis, gene and application thereof
ActiveCN103087998AIncrease productionEnhance expressive abilityBacteriaMicroorganism based processesAcyl Coenzyme A SynthetasesNucleotide
The invention relates to long-chain-acyl-coenzyme A synthetase for synthesizing metabolic cetyl-coenzyme A with the start that Bailing producing strain cordyceps sinensis hirsutella sinensis is participated in palmatic acid, a gene for coding the enzyme and an application thereof. The amino acid sequence of the long-chain-acyl-coenzyme A synthetase and the sequence represented by SEQ ID No. 1 or SEQ ID No. 3 have more than 90% of homology. According to the invention, the metabolic pathway for synthesizing cetyl-coenzyme A through palmatic acid is researched in principle in a detailed manner as follows: clone DNA of a nucleotide sequence provided by the invention can be transferred into engineering bacteria through transduction, conversion and combined transfer methods; gene expression is biologically synthesized by adjusting the cetyl-coenzyme A; high expressivity of host long-chain-acyl-coenzyme A synthetase is given; an effective way for enlarging the yield of the cetyl-coenzyme A is provided; and the cetyl-coenzyme A has a significant application prospect.
Owner:ZHEJIANG UNIV OF TECH +1
Compound type black acid dye
The invention relates to the field of printing and dyeing, and particularly relates to an acid dye, more particularly relates to a black acid dye. The invention aims to provide a compound type black acid dye which is formed by compounding a weak acid dye and an acid dye containing active groups so as to obtain excellent synchronism and levelling property as well as high wet color fastness during dyeing. The compound type black acid dye disclosed by the invention comprises a 1:2 asymmetric green light black metal complexation type neutral dye A, a 1:2 symmetric red light black metal complexation type dye B and a yellow acid dye C containing a cyanuric chloride active group. The compound type black acid dye disclosed by the invention not only effectively solves the problem of bad levelling property and bad reproducibility in traditional black dyeing but also achieves brighter coloring and better color saturation and expressivity, and simultaneously increases the quality of the product obtained.
Owner:HENGSHENG CHEM QIDONG
Enzyme for synthesizing and metabolizing inosine monophosphate of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis and application thereof
ActiveCN102690801AIncrease productionEnhance expressive abilityFungiBacteriaBiotechnologyBiosynthetic genes
The invention relates to an adenosine monophosphate (AMP) adenosine deaminase for synthesizing and metabolizing an inosine monophosphate from adenine nucleotide of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis from 'Bailing' production strain, a gene coding the enzyme and application thereof. The AMP adenosine deaminase comprises the proteins shown in SEQ ID No. 1 and SEQ ID No. 2, and the coding gene of the AMP adenosine deaminase corresponds to the nucleotide sequence shown in SEQ ID No. 3 and SEQ ID No.4. According to the invention, the metabolizing way for synthesizing the inosine monophosphate from the adenine nucleotide is researched in detail from the principle, and the cloned DNA containing the nucleotide sequence provided by the invention can be transferred to an engineering strain by transduction, transformation, and combined transfer; the expression of the gene is biologically synthesized by regulating the inosine monophosphate, the host inosine monophosphate is endowed with high expressivity, an effective way is provided for increasing the yield of the inosine monophosphate, and the invention has a great application prospect.
Owner:ZHEJIANG UNIV OF TECH +1
Enzyme for synthesizing and metabolizing adenine of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis, and gene and application of enzyme
InactiveCN102690797AEnhance expressive abilityIncrease productionFungiBacteriaBiotechnologyNucleotide
The invention relates to an adenine phosphoribosyltransferase for synthesizing and metabolizing an adenine from adenine nucleotide of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis from 'Bailing' production strain, a gene coding the enzyme and application of the adenine phosphoribosyltransferase. The amino acid sequence of the adenine phosphoribosyltransferase is shown in SEQ ID No. 1, and the coding gene of the adenine phosphoribosyltransferase corresponds to the nucleotide sequence shown in SEQ ID No. 2. According to the invention, the metabolizing way for synthesizing the adenine from the adenine nucleotide is researched in detail from the principle, and the cloned DNA containing the nucleotide sequence provided by the invention can be transferred to an engineering strain by transduction, transformation and combined transfer; through regulating the gene expression of the adenine biological synthesis, the host adenine is endowed with high expressivity, an effective way is provided for increasing the yield of the adenine, and the invention has a great application prospect.
Owner:ZHEJIANG UNIV OF TECH +1
Cordyceps Chinese Hirsutella uridine-cytidine kinase, coding gene and application thereof
ActiveCN103031285AIncrease productionEnhance expressive abilityBacteriaTransferasesBiotechnologyBiosynthetic genes
The invention relates to an enzyme from Bailing production bacterium Cordyceps Chinese Hirsutella for synthesizing metabolic pyrimidine nucleotide from pyrimidine nucleoside, a gene for coding the enzyme and application thereof. The enzyme is uridine-cytidine kinase which has more than 90% of homology with amino acid sequence disclosed as SEQ ID NO.1; and the coding gene has more than 90% of homology with nucleotide sequence disclosed as SEQ ID NO.2. The invention researches the metabolic pathway of pyrimidine nucleoside synthesized pyrimidine nucleotide in details in principle. The cloned DNA (deoxyribonucleic acid) comprising the nucleotide sequence provided by the invention can be transformed into engineering bacterium by transduction, conversion and conjugal transfer. By adjusting the expression of the pyrimidine nucleotide biosynthesized gene, the host pyrimidine nucleotide is endowed with high expressivity, thereby providing an effective way for enhancing the yield of the pyrimidine nucleotide and having great application prospects.
Owner:ZHEJIANG UNIV OF TECH +1
Cordyceps cytidine deaminase, coding gene and application thereof
ActiveCN103031295AIncrease productionEnhance expressive abilityBacteriaHydrolasesExpressivityNucleotide
The invention relates to an enzyme from Bailing production bacterium Cordyceps Chinese Hirsutella for synthesizing metabolic (desoxy) uridine from (desoxy) cytidine, a gene for coding the enzyme and application thereof. The enzyme is cytidine deaminase which has more than 90% of homology with amino acid sequence disclosed as SEQ ID NO.1; and the coding gene has more than 90% of homology with nucleotide sequence disclosed as SEQ ID NO.2. The invention researches the metabolic pathway of (desoxy) cytidine synthesized (desoxy) uridine in details in principle. The cloned DNA (deoxyribonucleic acid) comprising the nucleotide sequence provided by the invention can be transformed into engineering bacterium by transduction, conversion and conjugal transfer. By adjusting the expression of the (desoxy) uridine biosynthesized gene, the host (desoxy) uridine is endowed with high expressivity, thereby providing an effective way for enhancing the yield of the (desoxy) uridine and having great application prospects.
Owner:ZHEJIANG UNIV OF TECH +1
Expression animation transition method and system and intelligent terminal
The invention provides an expression animation transition method and system and an intelligent terminal, and the method comprises the steps: judging whether a currently played expression is interrupted or not when an expression conversion request is received; if the currently played expression is interrupted, calculating transition data according to the currently played expression and the requestexpression, and playing the request expression after the transition data is rendered; and if the currently played expression is not interrupted, directly playing the requested expression. According tothe method, transition data can be calculated and rendered while the previous expression animation is interrupted, so that the interrupted expression is naturally transited to the next expression, expression gradual change is realized, expression animation mutation is avoided, the expression is more vivid, the expressive force is stronger, and the display effect and the user experience are improved.
Owner:UBTECH ROBOTICS CORP LTD
Enzyme from Cordyceps sinensis Hirsutella sinensis for participating in anabolism to obtain xanthylic acid and application of enzyme
InactiveCN102703396AIncrease productionEnhance expressive abilityBacteriaMicroorganism based processesBiosynthetic genesXanthosine
The invention relates to an inosine monophosphate (IMP) dehydrogenase from 'Bailing' producing strain Cordyceps sinensis Hirsutella sinensis for participating in anabolism on IMP to obtain a xanthylic acid, a gene for encoding the dehydrogenase and application of the dehydrogenase. The IMP dehydrogenase comprises a protein shown by SEQ ID No.1 and a protein shown by SEQ ID No.2, and the coding gene of the protein shown by SEQ ID No.1 and the coding gene of the protein shown by SEQ ID No.2 respectively correspond to a nucleotide sequence shown by SEQ ID No.3 and a nucleotide sequence shown by SEQ ID No.4. According to the invention, the metabolic pathway of the IMP to synthetize the xanthylic acid is traversed from the principle; the cloning deoxyribonucleic acids (DNA) of the nucleotide sequences provided by the invention can be transferred into engineering bacteria through a transduction method, a transformation method and a conjugal transfer method; and through regulating the expression of the biosynthetic gene of the xanthylic acid, the high expressivity of a host xanthylic acid is endowed, and an effective way for increasing the yield of the xanthylic acid is provided. The IMP dehydrogenase has a great application prospect.
Owner:ZHEJIANG UNIV OF TECH +1
Enzyme for synthesizing and metabolizing xanthine of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis, and gene and application of enzyme
The invention relates to a guanine deaminase for synthesizing and metabolizing an xanthine from guanine of Cordyceps sinensis(Berk.)Sacc. Hirsutella sinensis from 'Bailing' production strain, a gene coding the enzyme and application of the guanine deaminase. The guanine deaminase comprises the proteins shown in SEQ ID No. 1 and SEQ ID No. 2, and the coding gene of the guanine deaminase corresponds to the nucleotide sequence shown in SEQ ID No. 3 and SEQ ID No.4. According to the invention, the metabolizing way for synthesizing the xanthine from the guanine is researched in detail from the principle, comprising: the cloned DNA of the nucleotide sequence provided by the invention can be transferred to an engineering strain by transduction, transformation, and combined transfer; the expression of the gene is biologically synthesized by regulating the xanthine, the host xanthine is given high expressivity, an effective way is provided to increase the yield of the xanthine, and the invention has a great application prospect.
Owner:ZHEJIANG UNIV OF TECH +1
Cordyceps Chinese Hirsutella uridylate-cytidylate kinase, coding gene and application thereof
ActiveCN103031286AIncrease productionEnhance expressive abilityBacteriaTransferasesExpressivityCytidylate kinase
The invention relates to an enzyme from Bailing production bacterium Cordyceps Chinese Hirsutella for synthesizing metabolic pyrimidine nucleoside diphosphate from pyrimidine nucleoside, a gene for coding the enzyme and application thereof. The enzyme is uridylate-cytidylate kinase which has more than 90% of homology with amino acid sequence disclosed as SEQ ID NO.1; and the coding gene has more than 90% of homology with nucleotide sequence disclosed as SEQ ID NO.2. The invention researches the metabolic pathway of pyrimidine nucleotide synthesized pyrimidine nucleoside diphosphate in details in principle. The cloned DNA (deoxyribonucleic acid) comprising the nucleotide sequence provided by the invention can be transformed into engineering bacterium by transduction, conversion and conjugal transfer. By adjusting the expression of the pyrimidine nucleoside diphosphate biosynthesized gene, the host pyrimidine nucleoside diphosphate is endowed with high expressivity, thereby providing an effective way for enhancing the yield of the pyrimidine nucleoside diphosphate and having great application prospects.
Owner:ZHEJIANG UNIV OF TECH +1
Nano antibody and preparation method and application thereof
ActiveCN109897106AGood water solubilityImprove patienceDigestive systemImmunoglobulins against cell receptors/antigens/surface-determinantsSolubilityExpressivity
The invention provides a nano antibody. As for the nano antibody, (1) an amino acid sequence shown in SEQ ID NO:1 is achieved; or (2) compared with the (1), the at least 70% identity, the at least 75%identity, the at least 80% identity, the at least 85% identity, the at least 90% identity, the at least 95% identity, and the at least 99% identity are achieved. According to the embodiments, the nano antibody is an antibody specifically targeting an ion channel TRPV3, the native conformation TRPV3 can be combined, and the nano antibody has the high water solubility, high tolerance, high stability, high antigen bondability, low immunogenicity, high tissue penetrating power, and high expressivity.
Owner:SHENZHEN HUADA GENE INST
ASGPR-targeted positive electron developer as well as preparation method and application thereof
ActiveCN109369755AHigh affinityImprove imaging effectSugar derivativesOrganic chemistry methodsExpressivityMda mb 231
The invention discloses an ASGPR-targeted positive electron developer as well as a preparation method and application thereof. The positive electron developer is capable of specifically targeting tumors such as HepG2, A549, MDA-MB-231, MCF-7 and the like with high ASGPR expressivity, has strong affinity with ASGPR expressed in tumor cells, has a good development effect and is taken as a potentialreceptor developer, and a novel way is provided for the PET / CT clinical diagnosis.
Owner:NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV
Halogen-free flame-proof panlite composition
InactiveCN101367988AColor expresses freelyNo degradation of physical propertiesExpressivitySulfonate
The invention provides a halogen-free fire-retarding ponlite polycarbonate resin composite, composed of 98.55-99.65 weight percent of ponlite polycarbonate resin, 0.05-0.5 weight percent of perfluoroalkane sulfonate and 0.2-1.0 weight percent of acrylic modified polytetrafluoroethylene. The environment friendly material has excellent fire-retarding performance and strong color expressivity; and the existing physical properties of the material are not degraded.
Owner:LG CHEM TIANJIN ENG PLASTIC
Cordyceps sinensis ribonucleotide reductase as well as coding gene and application thereof
InactiveCN103045548AIncrease productionEnhance expressive abilityBacteriaMicroorganism based processesNucleotideNucleoside diphosphate
The invention relates to ribonucleotide reductase from 'Bailing' producing strain cordyceps sinensis hirsutella sinensis for anabolism of deoxidized pyrimidine nucleoside diphosphate from pyrimidine nucleoside diphosphate, a gene coding the ribonucleotide reductase and application of the ribonucleotide reductase. The ribonucleotide reductase has over 90% of homology to the amino acid sequence shown by SEQ ID No.1 or SEQ ID No.3; and the coding gene has over 90% of homology to the nucleotide sequence shown by SEQ ID No.2 or SEQ ID No.4. According to the invention, the metabolic pathway for synthesizing deoxidized pyrimidine nucleoside diphosphate from pyrimidine nucleoside diphosphate is studied in details in principle, the cloning DNA (Deoxyribonucleic Acid) comprising the nucleotide sequence provided by the invention can be transferred into engineering bacteria through transduction, transformation and combined transfer, high expressivity of the host deoxidized pyrimidine nucleoside diphosphate is obtained by adjusting the expression of the deoxidized pyrimidine nucleoside diphosphate biosynthesis gene, an effective means of increasing output of the deoxidized pyrimidine nucleoside diphosphate is provided, and a significant application prospect is realized.
Owner:ZHEJIANG UNIV OF TECH +1
Cordyceps Chinese Hirsutella nucleoside diphosphokinase, coding gene and application thereof
ActiveCN103031287BEnhance expressive abilityIncrease productionBacteriaTransferasesBiosynthetic genesPhosphoric acid
The invention relates to an enzyme from Bailing production bacterium Cordyceps Chinese Hirsutella for synthesizing metabolic (desoxy) pyrimidine nucleoside triphosphate from (desoxy) pyrimidine nucleoside diphosphate, a gene for coding the enzyme and application thereof. The enzyme is nucleoside diphosphokinase which has more than 90% of homology with amino acid sequence disclosed as SEQ ID NO.1; and the coding gene has more than 90% of homology with nucleotide sequence disclosed as SEQ ID NO.2. The invention researches the metabolic pathway of (desoxy) pyrimidine nucleoside diphosphate synthesized (desoxy) pyrimidine nucleoside triphosphate in details in principle. The cloned DNA (deoxyribonucleic acid) comprising the nucleotide sequence provided by the invention can be transformed into engineering bacterium by transduction, conversion and conjugal transfer. By adjusting the expression of the (desoxy) pyrimidine nucleoside triphosphate biosynthesized gene, the host (desoxy) pyrimidine nucleoside triphosphate is endowed with high expressivity, thereby providing an effective way for enhancing the yield of the (desoxy) pyrimidine nucleoside triphosphate and having great application prospects.
Owner:ZHEJIANG UNIV OF TECH +1
Cordyceps Chinese Hirsutella 5'-nucleotidase, coding gene and application thereof
InactiveCN103031288AEnhance expressive abilityIncrease productionBacteriaHydrolasesBiotechnologyNucleotidase
The invention relates to a 5'-nucleotidase from Bailing production bacterium Cordyceps Chinese Hirsutella for synthesizing metabolic pyrimidine from pyrimidine nucleotide, a coding gene and application thereof. The amino acid sequence of the 5'-nucleotidase has more than 90% of homology with sequence disclosed as SEQ ID NO.1; and the coding gene has more than 90% of homology with nucleotide sequence disclosed as SEQ ID NO.2. The invention researches the metabolic pathway of pyrimidine nucleotide synthesized pyrimidine in details in principle. The cloned DNA (deoxyribonucleic acid) comprising the nucleotide sequence provided by the invention can be transformed into engineering bacterium by transduction, conversion and conjugal transfer. By adjusting the expression of the pyrimidine biosynthesized gene, the host pyrimidine is endowed with high expressivity, thereby providing an effective way for enhancing the yield of the pyrimidine and having great application prospects.
Owner:ZHEJIANG UNIV OF TECH +1
Cordyceps Chinese Hirsutella nucleoside diphosphokinase, coding gene and application thereof
ActiveCN103031287AEnhance expressive abilityIncrease productionBacteriaTransferasesBiosynthetic genesPhosphoric acid
The invention relates to an enzyme from Bailing production bacterium Cordyceps Chinese Hirsutella for synthesizing metabolic (desoxy) pyrimidine nucleoside triphosphate from (desoxy) pyrimidine nucleoside diphosphate, a gene for coding the enzyme and application thereof. The enzyme is nucleoside diphosphokinase which has more than 90% of homology with amino acid sequence disclosed as SEQ ID NO.1; and the coding gene has more than 90% of homology with nucleotide sequence disclosed as SEQ ID NO.2. The invention researches the metabolic pathway of (desoxy) pyrimidine nucleoside diphosphate synthesized (desoxy) pyrimidine nucleoside triphosphate in details in principle. The cloned DNA (deoxyribonucleic acid) comprising the nucleotide sequence provided by the invention can be transformed into engineering bacterium by transduction, conversion and conjugal transfer. By adjusting the expression of the (desoxy) pyrimidine nucleoside triphosphate biosynthesized gene, the host (desoxy) pyrimidine nucleoside triphosphate is endowed with high expressivity, thereby providing an effective way for enhancing the yield of the (desoxy) pyrimidine nucleoside triphosphate and having great application prospects.
Owner:ZHEJIANG UNIV OF TECH +1
Arginase derived from Cordyceps Chinese Hirsutella, coding gene and application thereof
ActiveCN104120118AExpand biological applicationsEnhance expressive abilityBacteriaHydrolasesBiotechnologyExpressivity
The invention relates to an arginase which is derived from Bailing production bacterium Cordyceps Chinese Hirsutella and participates in urea cycle mechanism, and a coding gene and application thereof. The amino acid sequence of the arginase is disclosed as SEQ ID No.1, and the nucleotide sequence of the coding gene is disclosed as SEQ ID No.2. By researching the L-arginine synthetic urea from principles in details, the invention provides an arginase which is derived from Bailing production bacterium Cordyceps Chinese Hirsutella and participates in urea cycle mechanism, and a coding gene thereof. The clone DNA (deoxyribonucleic acid) of the nucleotide sequence can be transferred into engineering bacterium by transduction, transformation and conjugal transfer; and the expression of the coding gene of the enzyme for catalyzing L-arginine to prepare corresponding urea is regulated to endow the host arginase with high expressivity, thereby providing an effective way for widening the biological application range of the arginase, and having great application prospects.
Owner:ZHEJIANG UNIV OF TECH +2
A DMD-based imaging preprocessing device and method
InactiveCN103716538BImprove dynamic rangeRealize the processing functionTelevision system detailsColor television detailsImaging qualityVisual perception
Owner:TIANJIN UNIV
Cordyceps Chinese Hirsutella carbamyl phosphate synthetase, coding gene and application thereof
ActiveCN103031281BIncrease productionEnhance expressive abilityBacteriaMicroorganism based processesNucleotideBiology
The invention relates to a carbamyl phosphate synthetase from Bailing production bacterium Cordyceps Chinese Hirsutella for synthesizing metabolic carbamyl phosphate from L-glutamic acid, a coding gene and application thereof. The amino acid sequence of the carbamyl phosphate synthetase has more than 90% of homology with sequence disclosed as SEQ ID NO.1; and the nucleotide sequence of the coding gene has more than 90% of homology with sequence disclosed as SEQ ID NO.2. The invention researches the metabolic pathway of L-glutamic acid synthesized carbamyl phosphate synthetase in details in principle. The cloned DNA (deoxyribonucleic acid) comprising the nucleotide sequence provided by the invention can be transformed into engineering bacterium by transduction, conversion and conjugal transfer. By adjusting the expression of the carbamyl phosphate biosynthesized gene, the host carbamyl phosphate is endowed with high expressivity, thereby providing an effective way for enhancing the yield of the carbamyl phosphate and having great application prospects.
Owner:ZHEJIANG UNIV OF TECH +1
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