5 results about "Guanine Nucleotides" patented technology

The application discloses a method for purifying and chromatographing and mass spectrometrically identifying deoxyguanosine or 3-hydroxybenzoic acid and application. After metabolites are extracted by a methanol method, UPLC-MS / MS-ultra high performance liquid chromatography and ABSCIEX QTRAP 5500 triple quadrupole tandem linear ion trap mass spectrometry are used to distinguish the metabolites by using liquid chromatography and mass spectrometry. The MS technology provides the ability to detect and quantify a large number of metabolites. The method optimizes the purification and mass spectrometric identification of the progeria markers in blood, identifies the structures of the differential markers deoxyguanosine and 3-hydroxybenzoic acid in blood by using the mass spectrometric method, and distinguishes the difficult-to-distinguish differential markers. The method for purifying and mass spectrometrically identifying the progeria differential markers in blood is simple and easy to operate, has small experimental dosage, good stability, is fast and accurate, and can make up for the deficiency in searching for the blood differential markers.

The invention discloses an immobilized nucleoside enzyme mutant and application thereof. The immobilized nucleoside enzyme mutant is prepared by covalently immobilizing a nucleoside enzyme mutant G178D / A202K on aminated magnetic Fe3O4 nanoparticles. The catalyst is applied to a hydrolysis reaction of guanine nucleotide (GMP), efficient catalysis is carried out in a water phase at 60-70 DEG C, the catalyst can be rapidly separated and recovered through an external magnetic field after the reaction, and then high-purity guanine is obtained through solid-liquid separation. The immobilized enzyme can be repeatedly used for more than or equal to 8 times, still keeps more than or equal to 80% of activity, remarkably improves the industrial efficiency of guanine preparation, and has the advantages of green process, simplicity and convenience in operation, low cost, easiness in industrialization and the like.

The present disclosure provides methods of treating subjects having glaucoma or elevated intraocular pressure (IOP), methods of identifying subjects having an increased risk of developing glaucoma or developing elevated IOP, methods of detecting human Rho Guanine Nucleotide Exchange Factor 12 (ARHGEF12) variant nucleic acid molecules and variant polypeptides, and ARHGEF12 variant nucleic acid molecules and variant polypeptides.

Polypeptide cosmetic compositions may be useful for addressing skin conditions. Such skin conditions include loss of firmness, lines and wrinkles, and other age-related skin changes associated with the general degradation of the skin dermis, which are often characterized by collagen loss and structural disorganization. Cosmetic compositions may comprise a polypeptide, a first polypeptide activator comprising a guanine nucleotide, and a second polypeptide activator comprising succinic acid. Preferably, the polypeptide has about 5 amino acid residues or less, and more preferably, the polypeptide comprises a tripeptide having an amino acid sequence of Ser-Thr-Pro-NH2. Preferred polypeptides may promote stimulation of collagen production in skin cells to which the cosmetic compositions are applied.

Polypeptide cosmetic compositions may be useful for addressing skin conditions. Such skin conditions include loss of firmness, lines and wrinkles, and other age-related skin changes associated with the general degradation of the skin dermis, which are often characterized by collagen loss and structural disorganization. Cosmetic compositions may comprise a polypeptide, a first polypeptide activator comprising a guanine nucleotide, and a second polypeptide activator comprising succinic acid. Preferably, the polypeptide has about 5 amino acid residues or less, and more preferably, the polypeptide comprises a tripeptide having an amino acid sequence of Ser-Thr-Pro-NH2. Preferred polypeptides may promote stimulation of collagen production in skin cells to which the cosmetic compositions are applied.