11 results about "Integrases" patented technology

An enzyme capable of mediating the integration of large DNA fragments into the mammalian genome and its applications are provided. Specifically, novel enzymes capable of integrating large DNA fragments into the genome are provided, including integrases as shown in SEQ ID NO: 1-7. Further, polynucleotides containing nucleotide sequences encoding integrases and host cells containing these polynucleotides are disclosed. A method for recombining target nucleic acids into the human genome using said integrase is also disclosed.

The present invention relates to a method for determining whether a sample comprises a pathogen or not, such as for determining whether a subject is likely to be infected with a pathogen, said pathogen expressing DNA modifying enzymes capable of cleaving and ligating DNA, such as topoisomerases and integrases. The invention also relates to kits of parts for performing such methods and uses thereof.

Disclosed are evolved Bxb1 and PhiC31 integrases that exhibit increased insertion activity and efficiency as compared to their wild-type counterparts and use of the evolved integrases to modify any sequence within the genome of a cell or subject.

The invention relates to the technical field of biology, and discloses an acinetobacter baumannii bacteriophage and application thereof, the acinetobacter baumannii bacteriophage is preserved in the China Center for Type Culture Collection (CCTCC), and the preservation number is CCTCC M20252580. The bacteriophage belongs to the brachycaudal bacteriophage family, the genome is double-stranded linear DNA, the full length is 41020bp, the bacteriophage does not contain integrase, virulence factors and drug-resistant genes, and the bacteriophage has high biological safety. The bacteriophage is high in titer, can specifically recognize and efficiently split acinetobacter baumannii including multi-drug-resistant strains, and has the capacity of permeating and removing bacterial biofilms at the same time. The invention also discloses application of the bacteriophage in preparation of antibacterial compositions, sterilization and disinfection preparations and food preservation and fresh-keeping. The bacteriophage provided by the invention is high in sterilization efficiency, strong in specificity, safe and non-toxic, and an effective biological solution is provided for prevention and control of acinetobacter baumannii drug resistance and biological membrane pollution.

This invention provides compositions, systems, and methods for modifying nucleic acids. Modification can be achieved using fusion proteins comprising nucleases, ligases, integrases, or combinations thereof. Nucleic acid modification may include ligation of a donor nucleic acid to a target nucleic acid. Nucleic acid editing may include replacing a portion of the target nucleic acid with a modified nucleic acid.

The disclosure relates to an enzyme capable of mediating the integration of large DNA fragments into mammalian genomes and uses thereof. Specifically, novel enzymes that can integrate large DNA fragments into genomes are provided, including integrases as shown in SEQ ID NO: 1-7. The disclosure further relates to polynucleotides containing nucleotide sequences encoding the integrases, and host cells containing these polynucleotides. The disclosure also relates to the integrases and methods for recombining target nucleic acids into the human genome by using the integrases.

The present disclosure provides, in part, methods for generating cell lines, e.g., for the production of recombinant protein, using integrases for genomic insertion into host cells.

The disclosure relates to an enzyme capable of mediating the integration of large DNA fragments into mammalian genomes and uses thereof. Specifically, novel enzymes that can integrate large DNA fragments into genomes are provided, including integrases as shown in SEQ ID NO: 1-7. The disclosure further relates to polynucleotides containing nucleotide sequences encoding the integrases, and host cells containing these polynucleotides. The disclosure also relates to the integrases and methods for recombining target nucleic acids into the human genome by using the integrases.

Disclosed are evolved Bxb1 and PhiC31 integrases, which exhibit increased insertion activity and efficiency as compared to their wild type counterparts, as well as a method for producing the same. And the use of the evolved integrase for modifying any sequence within the genome of a cell or subject.

The present disclosure provides, in part, recombinant, mutant Bxb1 integrases which are able to target pseudo-aft sites which are newly discovered "safe harbor" genomic locations, e.g., found in ribosomal DNA (rDNA), and method of engineering nucleic acids and genomic insertion of host cells using the same.

Provided herein are methods of releasing nucleic acids from a fixed biological sample comprising contacting the fixed tissue sample to an enzyme. The disclosure further provides methods to quantify and deconvolute a population of mRNA spliced variant isoforms from a cellular transcriptome. Additionally provided herein are methods delivering a barcode to a nucleic acid sample using integrases, nucleic acid samples having barcodes, and nucleic acid libraries thereof.