The invention belongs to
microarray chip based
DNA binding protein detection technology, and in particular relates to high-sensitivity high-flux
DNA binding protein detection technology by a
microarray chip. Double-stranded or single-stranded oligonucleotides are fixed on the
chip, and a
cell crude extract to be screened and the chip are directly incubated; then
exonuclease is put into the
system for incubation; and finally, a circular probe is added into the
system for rolling circle amplification. Specific lattice sequences of the chip capable of combining a
target protein can be taken as an extension primer for the rolling circle amplification; and sequences not combined with the
protein are digested by the
exonuclease, and cannot perform the rolling circle amplification, and finally amplified products and universal fluorescent probes are hybridized. The existence of the
DNA binding protein can be detected according to the existence of specific array dot signals on the chip; and the intensity of the lattice
fluorescence can quantitatively detect the content of the
target protein. The chip can realize the detection of the
DNA binding protein with super sensitivity, low cost, high specificity and
high flux, and can be widely applied to scientific research and clinical detection and diagnosis.