Chip for non-label detecting DNA bindin, preparation and use method thereof

A protein-binding and labeling detection technology, which is applied in the direction of biological testing, material inspection products, etc., can solve the problems of low-abundance protein loss, improve detection efficiency and sensitivity, disadvantages, etc., achieve comprehensive biological significance, save detection costs, and avoid pollution effect
CN1435492AInactive Publication Date: 2003-08-13SOUTHEAST UNIV
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHEAST UNIV
Publication Date
2003-08-13
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

A chip for the non-label detection of DNA bindin has the dual-chain oligonucleotide probes designed according to the specific DNA recognizing sequence for the DNA bindin to be detected. The probe A is fixed to the substrate, and a label is introduced to the probe B. The probe B and the solution of the DNA bindin are inculated together and then hybridized with chip. If DNA bindin exists, the probes A and B have very high binding efficiency and the label of probe B can be detected at the position of probe A.
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Description

technical field

[0001] The invention relates to a chip for non-marker detection of DNA binding protein and its preparation and application methods. The DNA-binding protein here mainly refers to regulatory factors with specific DNA-binding sequences. The chip can simultaneously detect multiple DNA-binding proteins, and has the advantages of high throughput, high sensitivity, direct view, simple preparation, low cost, and no need to label proteins. Background technique

[0002] The sequence-specific recognition and interaction of DNA and proteins play an important role in the regulation of biological gene expression, and the occurrence and development of almost all diseases are related to the abnormality of DNA-binding proteins. The study of DNA-binding proteins has attracted more and more attention, and has become one of the important contents of genome and proteome research. It is very important to detect the amount of one or more proteins in cells or diseased tissues. ...

Claims

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