The present invention discloses a method for detecting dsRNA virus of edible fungus, the dsRNA virus of edible fungus can be detected by use of dsRNA monoclonal antibody J2 by dot hybridization, and the method is as follows: an edible fungus detection sample is prepared, the edible fungus detection sample is applied to a hybridization membrane, a closing liquid is used to fast close redundant nonspecific DNA binding sites on the hybridization membrane, the hybridization membrane is respectively incubated with the dsRNA monoclonal antibody J2 and goat anti-mouse IgG for color development, color changes are viewed by naked eyes, and toxic situations of the edible fungus can be determined. A plurality of samples can be detected by the method simultaneously, and the method has the advantages of convenient operation, fast and convenient operation, no need of special instruments and equipment, clear positive signals, easy judgment and the like.