5 results about "Phytochrome" patented technology

The invention discloses Mn < 4 + >-doped oxyfluoride red fluorescent powder and a preparation method and application thereof, the chemical formula of the Mn < 4 + >-doped oxyfluoride red fluorescent powder is Mg7-xAxByGe2-yO10F2: zMn < 4 + >, A is at least one of Li, Sc and Zn, and B is at least one of Al, Ga, V, Mo, W, Ti, Si and Sn; 2x + 4y = px + qy, and p and q respectively correspond to chemical valence states of the element A and the element B; 0 < = x < = 0.8, 0 < = y < = 0.6, and 0 < z < = 0.5. The Mn < 4 + >-doped oxyfluoride red fluorescent powder is high in luminous efficiency, good in physical and chemical stability and excellent in quantum efficiency, the emission spectrum is well overlapped with the absorption spectrum of plant chlorophyll and phytochrome, the Mn < 4 + >-doped oxyfluoride red fluorescent powder can be applied to a light conversion film and a pc-LED lamp to supplement light for plants, and the illumination quality of the plants can be effectively improved.

The invention belongs to the technical field of gene engineering, and particularly relates to a light-controlled yeast two-hybrid system as well as an establishment method and application thereof. The light-controlled yeast two-hybrid system is obtained by integrating a phycocyanobilin synthesis module into a yeast strain containing a reporter gene and then transferring into a phytochrome A-LexA fusion expression vector and an AD-FHY1 fusion expression vector. The light-controlled yeast two-hybrid system provided by the invention well overcomes the defects that the existing light-controlled yeast two-hybrid system needs exogenous application of phycocyanobilin, the operation is more complex and the cost is higher, and does not cause that the phytochrome apoprotein expressed in yeast cells cannot realize conversion of two conformational forms of Pfr / Pr; and finally, the difference between the interaction intensity of the phytochrome in two conformational forms and the FHY1 signal protein cannot be distinguished.

This invention claims protection for a luminescent material for plant lighting and its preparation method. The chemical formula of the luminescent material is BaY. 2(1‑x) Al2Ga3O 12 :xBi 3+ ,yK + Where 0.005≤x≤0.03, 0.01≤y≤0.03. This luminescent material was synthesized via a high-temperature solid-state method with a sintering temperature of 1350℃-1450℃. This method is simple and easy to industrialize. The luminescent material, doped with Bi₂O₃, exhibits both blue and red light emission under ultraviolet wavelength excitation. The emission wavelengths of this luminescent material cover the absorption spectra of chlorophyll a, chlorophyll b, carotenoids, and phytochrome Pr, and its ability to simultaneously provide red and blue light emission has significant application value in plant growth lighting.

The invention discloses a red light reversible regulation gene expression system and application, the system comprises three plasmid vectors, respectively a plasmid pPA formed by fusing a PUF protein and a phytochrome PhyA, a plasmid pFE formed by fusing a shuttle protein FHY1 and eIF4E, and a downstream targeting regulation plasmid pNG formed by an NRE target and a downstream gene of interest GOI; according to the invention, the binding characteristic of RNA binding protein PUF and target NRE thereof is utilized, and the characteristic of dimerization of PhyA protein and ligand FHY1 thereof under red light is fused, so that a gene expression activation system capable of bidirectional light regulation is constructed, the PUF protein continuously and stably inhibits expression of downstream genes of interest, background leakage of the system is reduced from the source, and the expression of the downstream genes of interest is inhibited. Expression of downstream interested genes can be caused only under irradiation of red light, the expression can be rapidly closed by far-red light, and the expression of the genes can be accurately controlled through light; the system can be applied to tumor treatment.

The invention discloses a monoclonal antibody for detecting soybean phytochrome PhyB protein, a heavy chain variable region of the monoclonal antibody comprises CDR-H1, CDR-H2 and CDR-H3, corresponding amino acid sequences of the CDR-H1, the CDR-H2 and the CDR-H3 are respectively shown as SEQ ID NO: 4-6, a light chain variable region of the monoclonal antibody comprises CDR-L1, CDR-L2 and CDR-L3, corresponding amino acid sequences of the CDR-L1, the CDR-L2 and the CDR-L3 are respectively shown as SEQ ID NO: 7-9, and the light chain variable region of the monoclonal antibody comprises CDR-L1, CDR-L2 and CDR-L3. The method has the advantages that the affinity and the specificity to the PhyB protein of the soybean phytochrome are high, the PhyB protein can be accurately recognized, cross reaction with other proteins is avoided, a reliable tool is provided for expression dynamic tracking, subcellular localization and protein interaction research of the PhyB protein, and the application prospect is wide.