14 results about "Phytochrome" patented technology

The present application discloses a filamentous fungal mutant strain with high-efficient expression of superoxide dismutase and its application. The filamentous fungal mutant strain of the present application is formed by gene knockout of phytochrome genes PaPhy1 and / or PaPhy2 in the wild-type filamentous fungus Podospora anserina. The filamentous fungal mutant strain of the present application is a mutant strain with gene knockout of phytochrome gene PaPhy1, a mutant strain with gene knockout of phytochrome gene PaPhy2, or a mutant strain with gene knockout of phytochrome genes PaPhy1 and PaPhy2. The present application first discovers that the filamentous fungus Podospora anserina with gene knockout of phytochrome genes PaPhy1 and / or PaPhy2 can express superoxide dismutase and peroxidase more efficiently, which is of great significance for the high-efficient expression and preparation of superoxide dismutase and peroxidase.

The invention relates to the technical field of biological prevention and control, in particular to a method for analyzing rice leaf rhythm change after rice is infected by pyricularia grisea and application of the method in accurate prevention and control of rice blast. The analysis method comprises the following steps: (1) spraying a pyricularia grisea spore suspension on rice leaves; (2) carrying out whole-genome transcriptome analysis on the rice leaves, and carrying out clustering heat map; (3) analyzing expression conditions of the biological clock genes and the phytochrome related genes in the circadian rhythm pathway of the rice leaves, and analyzing the biological clock genes and the phytochrome related genes by a MetaCycle method; and (4) analyzing the deposition amount of H2O2 and callose in the rice leaves. Determining the dynamic change rule of the rice immune process caused by magnaporthe oryzae infection according to the method; in combination with the characteristics of small molecules, small-molecule pesticides are reasonably applied at a specific time point aiming at the rice blast, so that efficient and accurate prevention and control of the rice blast are realized.

The invention discloses Mn < 4 + >-doped oxyfluoride red fluorescent powder and a preparation method and application thereof, the chemical formula of the Mn < 4 + >-doped oxyfluoride red fluorescent powder is Mg7-xAxByGe2-yO10F2: zMn < 4 + >, A is at least one of Li, Sc and Zn, and B is at least one of Al, Ga, V, Mo, W, Ti, Si and Sn; 2x + 4y = px + qy, and p and q respectively correspond to chemical valence states of the element A and the element B; 0 < = x < = 0.8, 0 < = y < = 0.6, and 0 < z < = 0.5. The Mn < 4 + >-doped oxyfluoride red fluorescent powder is high in luminous efficiency, good in physical and chemical stability and excellent in quantum efficiency, the emission spectrum is well overlapped with the absorption spectrum of plant chlorophyll and phytochrome, the Mn < 4 + >-doped oxyfluoride red fluorescent powder can be applied to a light conversion film and a pc-LED lamp to supplement light for plants, and the illumination quality of the plants can be effectively improved.

The present invention relates to compositions and methods for modifying a phytochrome interaction factor (PIF) transcription factor gene in a plant to inhibit shade-avoiding response. The invention further relates to plants and plant parts produced using the methods and compositions of the invention.

The invention belongs to the technical field of gene engineering, and particularly relates to a light-controlled yeast two-hybrid system as well as an establishment method and application thereof. The light-controlled yeast two-hybrid system is obtained by integrating a phycocyanobilin synthesis module into a yeast strain containing a reporter gene and then transferring into a phytochrome A-LexA fusion expression vector and an AD-FHY1 fusion expression vector. The light-controlled yeast two-hybrid system provided by the invention well overcomes the defects that the existing light-controlled yeast two-hybrid system needs exogenous application of phycocyanobilin, the operation is more complex and the cost is higher, and does not cause that the phytochrome apoprotein expressed in yeast cells cannot realize conversion of two conformational forms of Pfr / Pr; and finally, the difference between the interaction intensity of the phytochrome in two conformational forms and the FHY1 signal protein cannot be distinguished.

The invention relates to the technical field of concrete preparation, and particularly discloses vegetation concrete and a preparation method thereof.The vegetation concrete is sequentially composed of a vegetation layer, a water retention layer and a water permeable layer from top to bottom; according to the plant-growing concrete disclosed by the invention, a seed premixing method and an intelligent layered pouring method are adopted, and the germination rate and the survival rate of plant seeds are remarkably improved through the water-retaining agent, the nutrient additive and a proper material ratio. The survival rate of the plants can reach 85% or above in severe environment areas such as side slopes, wastelands and mining areas, and is far higher than that of a traditional sowing mode. According to the method, not only is the greening speed increased, but also the greening coverage degree is effectively improved, a permeable layer and a water retention layer are integrated, the vegetation concrete has good water retention and water permeability by regulating and controlling the material ratio, water is stored in the concrete by introducing the water retention material such as polyacrylamide, and meanwhile, the greening coverage degree is effectively improved. The permeable structure on the bottom layer can effectively drain excessive water and prevent root rot.

The present application discloses a method and a phytochrome for preparing acylated phytochrome, comprising the following steps: subjecting an anthocyanin phytochrome dissolved in an organic solvent and an acylating agent containing a carboxyl group to an acylation reaction under the action of an acylating enzyme to obtain a reaction mixture; and separating the acylated phytochrome from the reaction mixture. By acylating some or all of the -OH on the phytochrome glycoside with an organic acid, the molecular polarity is reduced, and the steric hindrance effect of the acyl group protects the anthocyanin phytochrome; the acylating group associates with the chromophore molecule to form an acyl glycoside, reducing the possibility of bond breakage in the molecular structure and improving its own stability. In addition, the free carboxyl group in the phytochrome has an acidifying ability. In an aqueous solution environment, the proton dissociation of the free carboxyl group -COOH generates H+, thereby reducing the acidity and alkalinity of the solution and reducing the discoloration in an alkaline solution.

The invention discloses application of a plant pigment interaction factor gene PIF4 in regulating and controlling plant self-incompatibility. The plant self-incompatibility can be effectively inhibited by inhibiting PIF4 gene expression. According to the invention, the fact that the plant pigment interaction factor gene PIF4 can regulate and control S-RNase expression is determined for the first time, so that the self-incompatibility of plants is regulated and controlled; the application is easy to operate and good in effect, the self-pollination maturing rate can be increased by 33%, and the method has excellent breeding application prospects.

This invention claims protection for a luminescent material for plant lighting and its preparation method. The chemical formula of the luminescent material is BaY. 2(1‑x) Al2Ga3O 12 :xBi 3+ ,yK + Where 0.005≤x≤0.03, 0.01≤y≤0.03. This luminescent material was synthesized via a high-temperature solid-state method with a sintering temperature of 1350℃-1450℃. This method is simple and easy to industrialize. The luminescent material, doped with Bi₂O₃, exhibits both blue and red light emission under ultraviolet wavelength excitation. The emission wavelengths of this luminescent material cover the absorption spectra of chlorophyll a, chlorophyll b, carotenoids, and phytochrome Pr, and its ability to simultaneously provide red and blue light emission has significant application value in plant growth lighting.

The present invention discloses a Mn 4+ The invention relates to an activated deep red light emitting phosphor for plant growth and its preparation method and application, belonging to the technical field of solid luminescent materials. The chemical formula of the phosphor is La 2‑2x‑z A 2x Mg(Al 1 / 2‑x‑z W z ,Ta 1 / 2+x )O6:yMn 4+ , where A is one or more combinations of Ca, Sr, and Ba; the value range of x is 0 ≤ x ≤ 1 / 2; the value range of y is 0 < y ≤ 0.3; the value range of z is 0 ≤ z ≤ 1 / 2; and x + z ≤ 1 / 2. The synthesized phosphor of the present invention can effectively convert ultraviolet light and some visible light in sunlight into far-red light. It has high quantum efficiency, good water stability, and good overlap of its emission spectrum with the absorption band of plant phytochromes. When used in light-conversion thin films to supplement light for stem-leaf plants and fruit-bearing plants, it can effectively enhance photosynthesis efficiency, shorten the growth cycle, and improve plant yield and fruit quality. It has good application prospects in the field of plant cultivation.

The invention discloses a red light reversible regulation gene expression system and application, the system comprises three plasmid vectors, respectively a plasmid pPA formed by fusing a PUF protein and a phytochrome PhyA, a plasmid pFE formed by fusing a shuttle protein FHY1 and eIF4E, and a downstream targeting regulation plasmid pNG formed by an NRE target and a downstream gene of interest GOI; according to the invention, the binding characteristic of RNA binding protein PUF and target NRE thereof is utilized, and the characteristic of dimerization of PhyA protein and ligand FHY1 thereof under red light is fused, so that a gene expression activation system capable of bidirectional light regulation is constructed, the PUF protein continuously and stably inhibits expression of downstream genes of interest, background leakage of the system is reduced from the source, and the expression of the downstream genes of interest is inhibited. Expression of downstream interested genes can be caused only under irradiation of red light, the expression can be rapidly closed by far-red light, and the expression of the genes can be accurately controlled through light; the system can be applied to tumor treatment.

The invention discloses a monoclonal antibody for detecting soybean phytochrome PhyB protein, a heavy chain variable region of the monoclonal antibody comprises CDR-H1, CDR-H2 and CDR-H3, corresponding amino acid sequences of the CDR-H1, the CDR-H2 and the CDR-H3 are respectively shown as SEQ ID NO: 4-6, a light chain variable region of the monoclonal antibody comprises CDR-L1, CDR-L2 and CDR-L3, corresponding amino acid sequences of the CDR-L1, the CDR-L2 and the CDR-L3 are respectively shown as SEQ ID NO: 7-9, and the light chain variable region of the monoclonal antibody comprises CDR-L1, CDR-L2 and CDR-L3. The method has the advantages that the affinity and the specificity to the PhyB protein of the soybean phytochrome are high, the PhyB protein can be accurately recognized, cross reaction with other proteins is avoided, a reliable tool is provided for expression dynamic tracking, subcellular localization and protein interaction research of the PhyB protein, and the application prospect is wide.

This invention discloses a longevity biomarker based on serum metabolomics and its application, belonging to the field of biomedical technology. The longevity biomarker disclosed in this invention, based on serum metabolomics, includes mandelic acid, glutethimide, fenmetrazine, hydroxyheptanoic acid, norfloxacin, N-acetylserotonin, 3,4-methylenedioxyphenylamine, palmitoleic acid, cinconidine, gulonose, 5-aminopentanal, 12,13-dihydroxy-9Z-octadecenoic acid, anserine, (3Z)-phytochrome bile, phytocyanin, and L-selenomethylselenocysteine. This invention provides new biomarkers for diagnosing healthy aging and predicting human lifespan, and can be used to prepare products that predict the likelihood of longevity, showing promising applications in the field of anti-aging and health.

A photoacoustic contrast agent based on a reversible optical switch fusion protein, a preparation method therefor, and use thereof. The photoacoustic contrast agent comprises a phytochrome protein and a collagen-binding domain fused to the C-terminus of the phytochrome protein. The phytochrome protein is derived from Deinococcus radiodurans. The photoacoustic contrast agent combines the phytochrome protein derived from Deinococcus radiodurans and the collagen-binding domain, ingeniously integrating the optical switch function of DrBphP and the high affinity of the CBD for collagen. The DrBphP-CBD fusion protein retains the high affinity for type I collagen and type III collagen and its optical switch property. The photoacoustic contrast agent can be specifically attached to exposed collagen by utilizing the non-typical vascular structure of tumors and the excessive deposition of collagen in fibrotic diseases, thereby facilitating continuous delivery and retention in the affected tissue microenvironment and providing enhanced optical switch photoacoustic differential imaging for pathological section staining and in vivo imaging detection of diseases.