Rice flowering related protein and coding gene LHD3 thereof and application thereof

A protein and rice technology, applied in the fields of application, genetic engineering, plant gene improvement, etc., can solve the problems of ignorance of functional research, poor understanding of phytochrome C, limited resources of phytochrome C mutants, etc., and achieve the effect of broad application prospects

Inactive Publication Date: 2019-07-19
JIANGXI AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Due to the limited resources of phytochrome C mutants, only one insertion mutant has been reported,

Method used

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  • Rice flowering related protein and coding gene LHD3 thereof and application thereof
  • Rice flowering related protein and coding gene LHD3 thereof and application thereof
  • Rice flowering related protein and coding gene LHD3 thereof and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1. Obtaining and phenotypic analysis of mutant materials

[0020] Through EMS chemical mutagenesis of the japonica rice variety Nipponbare, a mutant lhd3 with a severely delayed heading date was screened, and the traits of this mutant have been stably inherited through multiple generations of selfing. The flowering time of the mutant was 197 days under long-day conditions and 145 days under short-day conditions, while the flowering times of normal Nipponbare rice were 90 days and 81 days, respectively. In addition, compared with the wild type under field conditions, the mutant also had lighter leaf color, and the chlorophyll content of the mutant was significantly lower than that of the wild type Nipponbare. All rice materials were planted in the experimental field of Jiangxi Agricultural University in Nanchang City, Jiangxi Province and the southern propagation base of Jiangxi Agricultural University in Sanya City, Hainan Province, under routine management. ...

Embodiment 2

[0022] Embodiment 2, population construction and genetic analysis

[0023] In order to avoid the influence of genetic background differences on heading, the mutant lhd3 was crossed with japonica rice varieties Nipponbare, Zhonghua 11 and Wuyunjing 7, and F 1 The plants all showed normal heading phenotype, which indicated that lhd3 was controlled by recessive nuclear gene. Statistics F 2 Segregation population segregation ratio (table 1), result shows, the segregation ratio of the plant of normal phenotype and the plant of late heading phenotype is close to 3:1 segregation through chi-square test, and this shows that the waxy reduction of lhd3 and premature senescence phenotype are caused by One-to-one single recessive nuclear gene control.

[0024] Table 1 Genetic analysis of rice brittle stalk mutant lhd3

[0025]

[0026]

Embodiment 3

[0027] Embodiment 3, fine mapping of LHD3 gene

[0028] In order to ensure the polymorphism of molecular markers, we selected the indica rice variety TN 1 Crossbreed with mutants to construct targeting populations. To avoid genetic differences affecting heading date sampling, we set the F 2 The extremely late heading individual plants were selected from the population for location. Using the SSR primers evenly distributed on the 12 rice chromosomes preserved in our laboratory to pair mutants with TN 1 Perform polymorphism screening. Then use 21 lhd3 / TN 1 Middle F 2 Linkage analysis was performed on the middle and extreme late heading single plants to preliminarily confirm the chromosomal position of the target gene. Genomic DNA was extracted by CTAB method. Specific steps are as follows:

[0029] ①. Weigh 0.1g of rice leaves and grind them into powder with liquid nitrogen, then add 600 μL of CTAB solution (2% (m / V) CTAB, 100mmol / L Tris-Cl, 20mmol / L EDTA, 1.4mol / L NaCl ...

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Abstract

The present invention discloses a rice flowering related protein and a coding gene LHD3 thereof and an application thereof in rice breeding. The provided rice flowering related protein has an amino acid sequence shown in SEQ ID NO:2. The present invention also provides the gene LHD3 encoding the above-mentioned protein and a nucleotide sequence of the gene LHD3 is shown in SEQ ID NO:1. The gene LHD3 is applied in improving rice heading stage and rice photomorphogenesis. The rice flowering gene LHD3 is isolated, cloned and identified, and besides, gene functions are verified according to a complementation experiment. Results of map-based cloning indicate that the gene encodes rice phytochrome C. The rice flowering related protein and the coding gene LHD3 thereof provide germplasm resourcesand theoretical support for breeding new rice varieties with early heading and good photomorphogenesis, and have broad application prospects.

Description

technical field [0001] The invention belongs to the field of plant genetic engineering. Specifically, the present invention relates to a method of cloning rice LHD3 (Late Heading Date 3) gene by map-based cloning technology, and identifying the function of the gene by transgene complementation experiment; meanwhile, it also relates to the regulation of photomorphogenesis of rice by using the gene, Used to improve rice varieties to increase yield. Background technique [0002] High yield is an eternal topic in rice breeding. Heading date is one of the most important agronomic traits of rice, which directly affects the length of growth period and the formation and yield of rice grains. Especially in double-cropping rice areas, the requirements for the heading date of rice are more stringent, and regulating the flowering time of rice is one of the important means to ensure the safe production of grain in my country. Therefore, the study of different flowering mutants is of g...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01H6/46
CPCC07K14/415C12N15/827
Inventor 徐杰邹国兴贺浩华黄永萍李婷林小丽姜志树朱昌兰傅军如贺晓鹏边建民
Owner JIANGXI AGRICULTURAL UNIVERSITY
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