The invention discloses a seeding and cultivation medium for clematis acerifolia, and relates to the technical field of cultivation. The culture medium comprises a bedrock layer, a root planting layer and a covering layer from bottom to top; the thickness of the bedrock layer is larger than 10 cm, the bedrock layer is composed of limestone, perlite and pumice, the volume ratio of the limestone to the perlite and the pumice is 1: 1, and the perlite and the pumice are in any ratio; the thickness of the root planting layer is 0.5 cm-1 cm, the root planting layer is composed of coco coir, perlite, pumice and Osmocote 318s, the volume ratio of the coco coir to the perlite and the pumice to the Osmocote 318s is 56: 56: 1, and the perlite and the pumice are in any ratio; and the thickness of the covering layer ranges from 0.5 cm to 1 cm, and the covering layer is composed of limestone chippings. According to the sowing and cultivation medium, the germination rate and the survival rate of seeds are remarkably increased, the growth speed of the seeds can be increased, the growth amount of 8-15 true leaves can be achieved in the first year, the plant specification is comparable to that of a field individual in 4 years-5 years, meanwhile, the flowering time is shortened, a small amount of seedlings bloom in the spring of the next year after one-year sowing, and the flowering time is shortened by at least four years compared with field individual flowering time.