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82results about How to "Maintain biological properties" patented technology
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Bioreactor for three-dimensional cell perfusion culture
InactiveCN103966095AStable flow ratePromote hyperplasiaTissue/virus culture apparatusSpecific use bioreactors/fermentersPerfusion CulturePeristaltic pump
The invention relates to a bioreactor for three-dimensional tissue cell perfusion culture. A reaction chamber, a liquid storage tank and a peristaltic pump are sequentially connected through transfusion pipes, wherein the reaction chamber comprises a box cover and a box body comprising lateral walls, a bottom wall, a cut-off device, an overflow insert plate, screen frames, a static pressure channel, a sluice channel and a draining channel; the cut-off device is a gate or a weir flow dam, and a plurality of uniformly arranged water holes are formed in the same plane along the longitudinal section of the cut-off device; an overflowing opening matched with the draining channel is formed in the lateral wall of the static pressure channel; and the screen frames are fixed on two lateral walls of the box body. The invention also relates to a bioreactor with a laminated structure, which can be used for solving the problem of cell death easily caused by non-uniform flow field and non-uniform nutritional transfer in the existing perfusion culture, is suitable for three-dimensional culture of tissue cells, can be used for improving the quality of tissue engineering products, and has the characteristics of good expansibility, strong universality and wide application range.
Owner:THE THIRD AFFILIATED HOSPITAL OF THIRD MILITARY MEDICAL UNIV OF PLA
Storage method of immune cells and cell freezing medium
InactiveCN105685017AGuaranteed sterilityAvoid safety hazardsDead animal preservationPeripheral blood mononuclear cellBlood plasma
The invention relates to the technical field of cell preservation, in particular to a storage method of immune cells and a cell freezing medium. The method includes: separating and collecting peripheral blood mononuclear cells, and adding the cell freezing medium to store the cells in a frozen manner, wherein the cell freezing medium comprises, by weight percentage, 0-62% of serum-free liquid culture medium, 0.5-1.5% of penicillin-streptomycin solution, 5-10% of dimethyl sulfoxide and 30-92% of autologous plasma. The storage method of the immune cells has the advantages that the method is simple and effective, the infectious disease risks of exogenous serum are avoided, the survival rate of the revived freezing-stored cells after induction culture is above 90%, the various biological indexes of the cells satisfy the requirements, and a good storage effect is achieved; the method can monitor and trace cell bank samples in real time, accurately record information such as cell storage positions, temperature and cell sources, each cell has a unique identifier, and the method is convenient to use and safe and reliable.
Owner:DONGGUAN BOALAI BIOLOGICAL TECH CO LTD
Preparation method of fish skin collagen
InactiveCN101948533AHigh yieldKeep dryConnective tissue peptidesVertebrate cellsAcetic acidCollagenan
The invention belongs to the field of processing fish skin collagen and particularly relates to a preparation method of fish skin collagen which can be used as a fish cell cultural carrier. The preparation method comprises the steps of raw material processing, extracting, salting and purification dialysis drying, wherein in the raw material processing step, absolute ether is refluxed at the temperature of 35 to 40 DEG C for 3 to 4h to remove the fat of fish skin, the fish skin is dried in the air, and fish skin residues are obtained; in the extraction steps, 0.5 to 0.6M acetic acid is added into the fish skin residues according to a solid to liquid ratio of 1:50 to 70 for leaching for 60 to 80h, and a 40-mesh sieve is used for filtering to obtain filtrate containing the fish skin collagen; and the steps are repeated once. In the invention, yield is improved on the basis of keeping the biological nature and pureness of the fish skin collagen; when the fish skin collagen obtained is used as a fish cell cultural carrier, cellular affinity is good, adherence speed is fast, cell growth is excellent, and stand density is high; therefore, the fish skin collagen is more advanced than cow skin collagen or mouse tail collagen.
Owner:SUZHOU UNIV
Culture method of mesenchymal stem cells
ActiveCN112522191AImprove proliferative abilityComplete formCulture processSkeletal/connective tissue cellsMesenchymal stem cellSecretion
The invention relates to the technical field of cell culture, and particularly relates to a culture method of mesenchymal stem cells. In the culture method, a serum-free culture medium optimized by the invention is used for culturing the mesenchymal stem cells. In addition, the invention further provides a better culture method for 3D culture. The cells obtained by the culture method disclosed bythe invention are strong in proliferation capacity, intact in shape and strong in secretion capacity, and are in accord with the international quality control standards of the mesenchymal stem cells.Meanwhile, according to the culture method disclosed by the invention, a large number of mesenchymal stem cells (particularly human umbilical cord mesenchymal stem cells) can be obtained by amplifyinga small number of mesenchymal stem cells under the conditions of small space occupation, less culture medium consumption, simpler and more convenient operation and greatly reduced workload; and a solid technical scheme and a theoretical basis are provided for obtaining a large number of high-quality mesenchymal stem cells (especially human umbilical cord mesenchymal stem cells) and secretions thereof in the clinical field.
Owner:YUNNAN KEY LAB OF PRIMATE BIOMEDICINE RES
Chemoselective Fluorgenic Molecular Linkers and Methods for Their Preparation and Use
ActiveUS20070224695A1Easy to useEasy to distinguishBiocideMicrobiological testing/measurementFluorescenceChemical compound
The present invention describes a bioconjugation strategy and compounds that are useful therein in which a fluorescent signal is produced when two molecular or supramolecular entities are linked by chemoselective combination of one linker having an azido or halide substituent group with another linker having a cyano or an alkyne substituent group. A kit is also provided.
Owner:SOUTH CAROLINA UNIV OF
Serum-free cryopreservation medium and method for peripheral blood monouclear cells
InactiveCN108651439ABiological Property GuaranteeReduced Possibility of ContaminationDead animal preservationPeripheral blood mononuclear cellCytotoxicity
The invention relates to a serum-free cryopreservation medium and method for peripheral blood monouclear cells. The serum-free cryopreservation medium comprises recombinant human interleukin-2, humanserum albumin, polyethylene glycol, trehalose and a basic culture medium or sodium chloride for injection. The serum-free cryopreservation medium has the advantages that the recombinant human interleukin-2 (IL-2) is added into the cryopreservation medium, so that the activity stability of immune cells cultured by thawed cells can be increased greatly, the high activity of original cells can be kept, the immune cells can well keep the physiological function and biological feature of the thawed cells, and the problem of direct large-scale amplification of the thawed cells can be solved effectively; the polyethylene glycol and the trehalose are added into the cryopreservation medium, the cryopreservation medium can replace an existing dimethyl sulfoxide-based (DMSO-based) cryopreservation medium, the toxicity of the cryopreservation medium to the cells is lowered effectively, cell stability is maintained, the direct application safety of the thawed cells is increased, and the thawed cellscan be directly used.
Owner:上海韵飞生物科技有限公司
Chemoselective fluorgenic molecular linkers and methods for their preparation and use
ActiveUS7745229B2Maintain biological propertiesImprove bindingMicrobiological testing/measurementChemiluminescene/bioluminescenceAzirineFluorescence
The present invention describes a bioconjugation strategy and compounds that are useful therein in which a fluorescent signal is produced when two molecular or supramolecular entities are linked by chemoselective combination of one linker having an azido or halide substituent group with another linker having a cyano or an alkyne substituent group. A kit is also provided.
Owner:SOUTH CAROLINA UNIV OF
Immune cell cryopreservation liquid, and preparation method, cryopreservation method and application thereof
InactiveCN107787959AAvoid shrinkageGood freezing effectDead animal preservationPhysiological functionMethyl cellulose
The invention discloses an immune cell cryopreservation liquid, and a preparation method, a cryopreservation method and an application thereof; the immune cell cryopreservation liquid includes the components by weight: 5%-15% of DMSO, 1%-5% of propylene glycol, 2%-8% of trehalose, 3%-8% of methyl cellulose, 0.3%-1.2% of glutathione, 0.06%-0.14% of sodium pyruvate, and 60%-80% of human donor plasma; the cryopreservation effect is quite good, the immune cells can be effectively protected from freezing injury, physiological functions and biological characteristics of the immune cells after resuscitation are maintained, cell biological characteristics are not changed, and the immune cell cryopreservation liquid can be used in clinical treatment; the immune cell cryopreservation liquid is suitable for long-term frozen preservation of human mononuclear cells and other kinds of immune cells.
Owner:广州市金航生物科技有限公司
Anti-tumor drug screening kit based on three-dimensional micro-tissue level and application method thereof
InactiveCN107557426AMaintain biological propertiesReliable drug susceptibility testing dataMicrobiological testing/measurementTumor/cancer cellsBalance saltCell culture media
The invention relates to an anti-tumor drug screening kit based on a three-dimensional micro-tissue level and an application method thereof. The anti-tumor drug screening kit comprises a three-dimensional cell culture material, a tissue cell dispersion reagent, a proteinase inhibitor, a basic cell culture medium, blood serum, antibiotics, a balance salt solution, a cell activity detection reagentand a cell filter screen. The application method comprises the following steps: dispersing tumor tissues of a patient to obtain tissue blocks, tissue fragments, cell aggregates and / or dispersed singlecells; then culturing the dispersed tumor tissues of the patient and a cell sample through a three-dimensional culture method and enabling the cells to grow in a three-dimensional space to form a spherical structure or an irregular three-dimensional structure with a stereoscopic shape; finally, carrying out a drug sensitivity test on tumor micro-tissues obtained by three-dimensional culture. Theanti-tumor drug screening kit based on the three-dimensional micro-tissue level is simple to operate and is suitable for the drug sensitivity tests of various solid tumors.
Owner:宋焱艳
Serum-free culture medium for mesenchymal stem cells and application thereof
ActiveCN110331130AShort cycleImprove consistencyCulture processSkeletal/connective tissue cellsHeterologousCell cycle
The invention relates to a serum-free culture medium for mesenchymal stem cells and application thereof. The serum-free culture medium comprises a basal culture medium and additive components. The additive components comprise the following components: 2'-Deoxyadenosine, 2'-Deoxycytidine-HCl, 2'-Deoxyguanosine, L-glutamine, human serum albumin, recombinant human transferrin, recombinant human insulin, rhPDGF-BB, rhFGF-b, rhTGF-beta1, rhEGF, sodium selenate and hydrocortisone. The serum-free culture medium disclosed by the invention does not contain animal-derived heterologous component such asbovine serum, and can effectively replace serum to culture bone marrow mesenchymal stem cells; and due to the synergistic effect of the additive components, the culture medium has the advantages of shortening cell cycle, rapid proliferation and good cell consistency while culturing bone marrow mesenchymal stem cells, and can effectively maintain the molecular characteristics and differentiation potential of the bone marrow mesenchymal stem cells. The bone marrow mesenchymal stem cells obtained by the culture medium are suitable for further scientific research and clinical application research.
Owner:苏州依科赛生物科技股份有限公司
Multi-enzyme system for separating different tissues-derived primary culture cells of normal human and mammal, application and related kit thereof
InactiveCN101914504AEasy to freezePromote recoveryHydrolasesNervous system cellsBiotechnologyNeutral protease
The invention relates to a multi-enzyme system for separating different tissues-derived primary culture cells of a normal human and a mammal. The system comprises a plurality of 0.0125 to 0.125 weight percent of trypsin, 0.02 to 0.2 weight percent of collagenase I-IV, 0.0015 to 0.015 weight percent of hyaluronidase, 0.02 to 0.2 weight percent of neutral protease, 0.0015 to 0.015 weight percent of deoxyribonuclease I, 1 to 10 U / ml papain, 0.015 to 0.15 weight percent of pronase, 5 to 50 U / ml elastase and 0.01 to 0.1 weight percent of separase. The invention also relates to the application of the multi-enzyme system and a kit formed by the multi-enzyme system. The multi-enzyme system of the invention has the characteristics of reasonable design and capacity of separating the different tissues-derived primary culture cells of the normal human and the mammal highly specifically and highly sensitively; and the separated primary culture cells have the characteristics of high survival rate, high purity, easy cryopreservation and recovery, can be used for medicament experimental evaluation, medicament toxicity test and medicament therapeutic judgment, and are suitable for large-scale popularization and application.
Owner:刘东旭
Cholesterylchitosan nano carrier as well as medicament-carried nano particle and preparation method thereof
InactiveCN101850122AImprove biological activityMaintain biological propertiesPharmaceutical non-active ingredientsAntineoplastic agentsPolymer scienceChitosan nanoparticles
The invention relates to a selective cholesteryl chitosan nano particle and a preparation method thereof. The preparation method comprises the following steps of: preparing cholesteryl succinate (CHS) and phthaloyl chitosan (PHCS) by using cholesterol, succinic anhydride, phthalic anhydride and chitosan as raw materials and then reacting the CHS and the PHCS to prepare the cholesteryl chitosan nano particle, wherein the particle size is 100-200 nm, the molar ratio of the cholesterol to the succinic anhydride is 1-30:3-90, and the molar ratio of the chitosan to the phthalic anhydride is 1-60:3-180. An amphiphilic polymer obtained with the preparation method comprises a hydrophilic polysaccharide skeleton and a hydrophobic cholesterol branched chain and can automatically gather in a water medium to form the nano particle with a nucleus-shell type structure; and the nano particle has positive charge, is beneficial to the entrapment of hydrophobic medicaments, albumen and DNA, and has the advantages of simple preparation method, good repeatability, easy industrial production, high medicament-carrying efficiency and favorable slow-releasing effect.
Owner:INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
Multi-enzyme system used for separating patient tumor tissue-originated primary cells, application method thereof, purpose thereof, and relative kit thereof
InactiveCN102373186AImprove the survival rate of cultureHigh culture purityHydrolasesTumor/cancer cellsPrimary cellCell separation
The invention relates to a multi-enzyme system used for separating patient tumor tissue-originated primary cells. The system comprises components of, by weight: 0.02 to 0.2% of collagenase I-IV, 0.001 to 0.01% of hyaluronidase, 0.01 to 0.1% of trypsin, 0.02 to 0.2% of proteinase neutral, 0.001 to 0.01% of deoxyribonuclease I, and 5 to 50U / ml of elastase. The invention also relates to an application method of the system, an application of the system, and a kit formed by the system. The multi-enzyme system is advantaged in reasonable design. With the system, patient tumor tissue-originated primary cells can be separated with high specificity and high sensitivity. The separated primary cells are characterized by high survival rate, high purity, easy cryopreservation and easy resuscitation. The system and the kit can be used in medicine experimental evaluation, medicine toxicity testing, and medicine therapeutic performance determination. The system, the method and the kit are suitable to be widely popularized.
Owner:刘东旭
Naked mole rate Schwann cell culture method
ActiveCN106754716ANormal functional activityGuaranteed Biological PropertiesCell dissociation methodsCulture processBiological propertyMammal
The invention relates to the technical field of cell biology, and in particular to a naked mole rate Schwann cell separation & purification and culture method. According to the method provided by the invention, Schwann cells are separated & purified and cultured from marrow DRG (dorsal root ganglion) of a fetal naked mole rate with the comprehensive use of a plurality of mediums, so that a reasonable method suitable for culturing the Schwann cells of a heterothermic mammal of rodent species, namely the naked mole rate, is found out. With the application of the method provided by the invention, a great amount of naked mole rate Schwann cells, which are normal in functions and activity, are conveniently, efficiently and economically obtained; the cells, when cultured under a hypoxia condition, can still reserve biological characteristics under an in-vivo state in an in-vitro environment, so as to facilitate further research, which is directly conducted in a pure in-vitro cell culture model, on special physiological functions of the naked mole rate Schwann cells; therefore, important theoretical bases are provided for researching a biological mechanism therein and for application to related clinical fields.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY +1
Mesenchymal stem cell serum-free culture medium and application thereof
ActiveCN110938590AClear ingredientsRule out instabilityCulture processSkeletal/connective tissue cellsTransferrinMolecular biology
The invention relates to a mesenchymal stem cells serum-free culture medium. The serum-free culture medium comprises a basal culture medium and an additive component, wherein the additive component comprises the following components: rhEGF, rhVEGF, rhPDGF-BB, rhFGF-b, rhTGF-beta1, human serum albumin, recombinant deferritin, a recombinant human insulin growth factor and hydrocortisone. The invention also provides an application of the mesenchymal stem cell serum-free medium. The composition is clear and stable; the instability of serum culture is eliminated, and meanwhile, all factors have thesynergistic effect, the umbilical cord mesenchymal stem cells can be efficiently amplified, the cell cycle is shortened, the proliferation is rapid, the yield of three generations of cultured cells reaches 1.5 times or more of that of serum culture, the cell consistency is good, and the biological characteristics and differentiation potential of the umbilical cord mesenchymal stem cells can be efficiently maintained due to the synergistic effect of all factors.
Owner:苏州依科赛生物科技股份有限公司
Carrier-free cell sheet and preparation method and application thereof
ActiveCN103642749AMaintain biological propertiesAvoid excessive differentiationVertebrate cellsArtificial cell constructsSupporting cellTissue engineering
The invention belongs to the field of tissue engineering, and particularly relates to a carrier-free cell sheet and a preparation method and application thereof. The preparation method of the carrier-free cell sheet comprises the steps of transfecting the suicide gene into a supporting cell; planting the supporting cell with the transfected suicide gene at the bottom of a culture container; planting target cells on the supporting cell; after realizing good fusion between the target cells and establishing intercellular connection, starting the suicide gene in the supporting cell for apoptosis to obtain a carrier-free cell sheet containing pure target cells. By adopting the method provided by the invention, a carrier-free cell sheet with good biological characteristics can be obtained, which is a new breakthrough in establishing a carrier-free cell sheet in the field of tissue engineering; meanwhile, the product also provides a feasible scheme for clinical disease treatment. The principle is scientific and reliable, and the process is simple and flexible.
Owner:JINAN UNIVERSITY
Exosome composition and method of manufacture
PendingUS20200230174A1Promote differentiationImprove performancePowder deliveryMammal material medical ingredientsBiomedical engineeringExosome
Compositions of exosomes are provided that include a plurality of exosomes and a biocompatible cryoprotectant, such that the exosomes are suspended in the biocompatible cryoprotectant as a colloidal suspension of exosomes. Preferably, the cryoprotectant is a carboxylated E-poly-1-lysine (COOH-PLL) cryoprotectant, but other moieties might be extended to the claim of hybridization polymers to incorporate preferred embodiments for lineage and tissue specific intentions. The colloidal suspension of exosomes can be frozen at −65 degrees C. or colder and thereafter stored as a frozen composition of exosomes or can be freeze-dried and thereafter stored at ambient conditions in a vacuum sealed container. Also provided are kits comprising the composition of exosomes and methods of making the compositions of exosomes.
Owner:VIVEX BIOLOGICS GRP INC
Pelodiscus sinensis heart cell continuous cell line and establishing method and ultra-low-temperature cryopreservation method thereof
ActiveCN105039241AMaintain biological propertiesMeet resourcesDead animal preservationArtificial cell constructsHeart cellsGermplasm
The invention relates to a pelodiscus sinensis heart cell continuous cell line which is preserved in the common microorganism center of China Committee for Culture Collection of Microorganisms and has a preservation number of CGMCC No.10597, and further relates to an establishing method and ultra-low-temperature cryopreservation method of the pelodiscus sinensis heart cell continuous cell line. The establishing method includes the following steps of firstly, conducting primary culture, wherein a pelodiscus sinensis heart is cut into tissue small pieces after being preprocessed, trypsin is inoculated into a culture bottle after being digested at the room temperature, and subculture is not started until cells are fully laid on 90% or more of the bottle bottom; secondly, conducting subculture, wherein air is blown on the bottle bottom through a trypsin digesting method to make adherent cells fall down so that subculture can be conducted. By means of the established pelodiscus sinensis heart cell continuous cell line, the research on viral diseases of pelodiscus sinensis and other turtle animals and the research on related therapy drugs for instance the analysis of chromosome and the research of iridescent virus can be conducted on the level of molecular cells, and the requirements for pelodiscus sinensis germplasm resource conservation and theoretical research and application can be met.
Owner:ZHEJIANG WANLI UNIV
Culture and cryopreservation method of amniotic mesenchymal stem cells
ActiveCN111139221AHigh yieldImprove survival rateCell dissociation methodsCulture processStem Cell IsolationNeutral protease
The invention relates to a culture and cryopreservation method of amniotic mesenchymal stem cells. The culture method comprises amniotic tissue separation, amniotic mesenchymal stem cell separation, P0 generation amniotic mesenchymal stem cell culture and amplification culture. According to the culture method of the amniotic mesenchymal stem cells, at a separating stage of the amniotic mesenchymalstem cells, a special mixed enzyme digestive juice system (the final concentrations of components in the digestive juice are as follows: 1.5-2U / mL of neutral protease, 0.5mg / mL of deoxyribonuclease Iand 1mg / mL of collagenase IV) is adopted for digestion, so that the amniotic mesenchymal stem cells can be more effectively separated from amniotic tissues, and then the yield of P0 generation cellsis obviously improved. Compared with conventional two-step digestion, the culture method provided by the invention has the advantages that a one-step digestion method is adopted, operation is more convenient, and the cultured amniotic mesenchymal stem cells have good activity, high yield, high purity and good repeatability.
Owner:赛瑞诺(北京)生物科技有限公司
Mesenchymal stem cell culture medium
PendingCN112608894APromote growthEasy to synthesizeCell dissociation methodsCulture processAntioxidantStem cell culture
The invention belongs to the technical field of biology, and particularly discloses a mesenchymal stem cell culture medium. The mesenchymal stem cell culture medium comprises a basal culture medium and additives, wherein the additives comprise non-essential amino acids, a pH regulator, antibiotics, organic substance components for promoting cell membrane synthesis, cell matrix components, cell growth factors, vitamins, trace elements and an antioxidant. The mesenchymal stem cell culture medium can provide nutrient substances required by cell growth for stem cell growth, maintain the cell growth environment and the cell activity, promote stem cell division and proliferation, increase the stem cell proliferation speed and shorten the stem cell culture time; meanwhile, stem cell differentiation can be inhibited, but the differentiation potential of stem cells is not influenced; after the culture medium is used for carrying out passage on the stem cells for multiple times, the stem cells can still keep the original biological characteristics and multidirectional differentiation potential.
Owner:任建华
Mouse fat stem cell culture solution
InactiveCN102433298ASimple ingredientsLow costSkeletal/connective tissue cellsL-glutamineBasic fibroblast growth factor
The invention discloses a mouse fat stem cell culture solution. The culture solution consists of the following components: 20 percent of fetal calf serum, 100 IU / ml of penicillin, 100 mug / ml of streptomycin, 2 mmol / L of L-glutamine, 10 ng / ml of basic fibroblast growth factor, 50 mug / ml of vitamin C, and the balance of Dulbecco's modified eagle medium F12 (DMEM / F12). The culture solution has simple component and low cost, can effectively maintain in vitro long-term propagation of mouse fat stem cells, and can well maintain the biological properties of the fat stem cells.
Owner:ANHUI AGRICULTURAL UNIVERSITY
Method for proliferating avian influenza virus by adopting MDCK cell line
PendingCN111662882AEfficient and stable toxin productionHigh agglutination titerSsRNA viruses negative-senseCell dissociation methodsProliferative capacityPrimary cell
The invention relates to a method for proliferating avian influenza virus by adopting an MDCK cell line. The method comprises the following steps: domesticating adherent MDCK cells into a serum-free full-suspension culture type MDCK cell line which is stably proliferated, inoculating a bioreactor with the MDCK cell line, completing primary cell culture, preparing avian influenza H5N1 subtype virusseed viruses for suspension MDCK cell culture, inoculating suspension MDCK cells with the avian influenza H5N1 subtype virus seed viruses, performing culturing, and separating and purifying virus particles. The cells obtained by domestication are stable in passage, good in cell morphology, high in quality, capable of keeping sensitivity and biological characteristics to viruses, high in virus multiplication capacity and capable of effectively multiplying avian influenza virus strains.
Owner:ZHAOQING DAHUANONG BIOLOGIC PHARMA
Culture medium for obtaining mesenchymal stem cells and exosomes thereof and preparation method of culture medium
PendingCN114480273ANormal growthPromote division and proliferationCell dissociation methodsCulture processAntioxidantMesenchymal stem cell
The invention discloses a culture medium for obtaining mesenchymal stem cells and exosomes thereof and a preparation method of the culture medium. DMEM / F12 is used as a basic culture medium, and the following components are added: 10-22 g / mL of a serum substitute, 20-55 ng / mL of a growth promoting factor, 5-10 ng / mL of a muscle cell repair factor HCGF, 5-16 mM of an anti-aging factor, a buffer agent, 0.5-3 mg / L of an antioxidant, 1-20 mg / L of vitamins, antibiotics, 5-65 mg / L of trace elements and 1-16 [mu] g / mL of a protective agent. The mesenchymal stem cell culture medium is definite in component, free of serum components, controllable in quality and capable of being produced on a large scale, stem cells can normally grow when the mesenchymal stem cell culture medium is used for in-vitro culture of the stem cells, moreover, the mesenchymal stem cell culture medium is beneficial to division and proliferation of the stem cells, the stem cell amplification rate is greatly increased, and the mesenchymal stem cell culture medium is suitable for large-scale production. Compared with the prior art, the stem cell culture medium has the advantages that stem cell culture time is shortened, and stem cells still can keep original biological characteristics and multidirectional differentiation potential after being subjected to multiple passage times by the aid of the stem cell culture medium.
Owner:杭州荣泽生物科技集团有限公司
Rapid propagation method for stem segments of Dendrobium huoshanense
InactiveCN105532457AEasy to get materialsThe process is simplePlant tissue cultureHorticulture methodsPlant cultivationSocial benefits
The invention discloses a rapid propagation method for stem segments of Dendrobium huoshanense, belonging to the technical field of plant cultivation. The method comprises the following steps: explant preparation, induced culture, enrichment culture, and strong seedling rooting culture. According to the invention, materials are easily available and are not limited by seasons; a stem segment culture manner of bud generation from buds has simple generating process and can well maintain biological characters of female parentd; thus, medicinal values of Dendrobium huoshanense are guaranteed. The propagation method is simple and practicable and solves the technical problems of insufficient explant sterilization in asexual propagation of Dendrobium huoshanense, slow protocorm differentiation, long propagation cycle, high production cost, etc. The rapid propagation method provided by the invention can be applied in actual production, is capable of providing guarantee for planting development of Dendrobium huoshanense, and has good economic benefits and social benefits.
Owner:ANHUI HUOSHAN NONGBOLE DEV CO LTD
Domestication method of full suspension culture-type MDCK cell line
PendingCN111676185AHigh densityFully contactedSsRNA viruses negative-senseViral antigen ingredientsBiotechnologyEmbryo
The invention relates to the field of cell culture, in particular to a domestication method of a full suspension culture-type MDCK cell line. According to the domestication method, adherent MDCK cellsare domesticated into a low-serum full suspension culture-type MDCK cell line and a serum-free full suspension culture-type MDCK cell line, so that traditional production of an avian influenza virusthrough chicken embryos can be replaced, the subsequent separation and purification efficiency is improved, further upgrade of a process is promoted, a culture process is also simplified while the cost is reduced, and the domestication method is easier to industrialize.
Owner:ZHAOQING DAHUANONG BIOLOGIC PHARMA
Naked mole rat microglial cell culture method
ActiveCN105695410AGuaranteed normal proliferationGuaranteed activityNervous system cellsBiological propertyMammal
The invention relates to the technical field of cell biology and in particular relates to a separation, purification and culture method of naked mole rat microglial cells. The microglial cells are separated from cerebral cortices of newly-born naked mole rats and are purified and cultured by comprehensively utilizing a plurality of types of culture mediums, and a reasonable culture method applicable to poikilothermal rodent mammal naked mole rat microglial cells is explored. The method provided by the invention can be used for simply, conveniently, efficiently and economically obtaining a lot of naked mole rat microglial cells with normal functional activity, and cells can still keep biological characteristics under an in-vivo state in an in-vitro environment through culture under a low-oxygen condition, so that special physiological functions of the naked mole rat microglial cells can be conveniently and directly researched in a pure in-vitro cell culture model, and furthermore, an important theoretical basis is provided for exploring a biological mechanism and applying the biological mechanism to clinically relative fields.
Owner:SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
A serum-free medium for mesenchymal stem cells and its use
ActiveCN110938590BReduce instabilityImprove consistencyCulture processSkeletal/connective tissue cellsPancreatic hormoneCultured cell
The invention relates to a mesenchymal stem cells serum-free culture medium. The serum-free culture medium comprises a basal culture medium and an additive component, wherein the additive component comprises the following components: rhEGF, rhVEGF, rhPDGF-BB, rhFGF-b, rhTGF-beta1, human serum albumin, recombinant deferritin, a recombinant human insulin growth factor and hydrocortisone. The invention also provides an application of the mesenchymal stem cell serum-free medium. The composition is clear and stable; the instability of serum culture is eliminated, and meanwhile, all factors have thesynergistic effect, the umbilical cord mesenchymal stem cells can be efficiently amplified, the cell cycle is shortened, the proliferation is rapid, the yield of three generations of cultured cells reaches 1.5 times or more of that of serum culture, the cell consistency is good, and the biological characteristics and differentiation potential of the umbilical cord mesenchymal stem cells can be efficiently maintained due to the synergistic effect of all factors.
Owner:苏州依科赛生物科技股份有限公司
Separation and culture method of adipose-derived stem cells
ActiveCN110079497APotential for differentiationReduce dosageCell dissociation methodsCulture processDigestionCell separation
The invention discloses a separation and culture method of adipose-derived stem cells. The method comprises the following steps of (1) collecting adipose tissues; (2) adding collagenase into the adipose tissues obtained in the step (1) for digestion to obtain the adipose-derived stem cells; (3) enriching the adipose-derived stem cells obtained in the step (2); and (4) conducting purification and subculture on the adipose-derived stem cells obtained in the step (3). In the step (2), an animo acid sequence of the collagenase is as shown in Seq ID No.5; a volume percent fraction of the adipose tissues in a digestion system is 40-60%; the content of the collagenase in the digestion system is 0.20-0.30 g / L; and digestion time is 15-30 min. The method has the advantages that the dosage of the collagenase is little, and the separation efficiency of the adipose-derived stem cells is high, so that the method has important values for scientific research and large-scale separation and culture ofadipose-derived stem cells.
Owner:济南赛尔生物科技股份有限公司
Preparation method of glue adhesion amnion
InactiveCN100479867CGood biocompatibilityHigh bonding strengthProsthesisEpitheliumBiological dressing
Owner:CHONGQING MEDICAL UNIVERSITY
Efficient isolation and purification method for Chenopodium quinoa
InactiveCN110637689AMaintain biological propertiesReduce the chance of mildewPlant cultivationCultivating equipmentsAnthesisCellophane
The invention belongs to the technical field of crop breeding, and provides an isolation and purification method for Chenopodium quinoa, and aims at solving the problems that Chenopodium quinoa growsmildew easily, the grains are not full and the Chenopodium quinoa is sterile at high temperature. A nonwoven fabric material is made into an isolation bag with a closed bottom, an opening at the upperend of the isolation bag is provided with a contraction rope, and two sides of the isolation bag are provided with cellophane observation windows; during the florescence of Chenopodium quinoa, branches and leaves were removed from top to bottom, and 2-3 cm of a trunk is exposed; the top end is sleeved with the isolation bag; after the top end is sleeved for 15-20 days, main ears fully fill the bag, the isolation bag is removed, pruning is performed downwards from the top end to remove branches and leaves and expose 2-3 cm of the trunk, secondary sleeving is performed; after the bag is changed, the bag is removed according to the flowering period, the single plant with the main ears sleeved are harvested in the mature period. The structure and method have no influence on the grain fullnessand the yield of Chenopodium quinoa, and can meet the requirements of subsequent research on the seed quantity. The problems that the inflorescences easily grow mildew during isolation and purification sleeving so that the seed setting amount is low, the grains are not full and sterility is caused due to the high temperature are solved.
Owner:AGRI BIOTECH RES CENT OF SHANXI PROVINCE
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