41results about How to "Guaranteed normal proliferation" patented technology

The invention relates to chimeric antigen receptors (CAR), particularly relates to dual-signal independent chimeric antigen receptors (dsCAR), and also relates to immune response cells of the dual-signal independent chimeric antigen receptors (dsCAR) and uses of the immune response cells in preparation of drugs for treatment of malignant tumor and virus infected diseases. In detail, the dual-signal independent chimeric antigen receptors (dsCAR) can respectively identify two different family antigens of tumor cells and can respectively transmit two T-cell-activation related signals. One of the CAR can transmit a first T-cell-activation related signal by combing a ligand of a tumor specific antigen or a tumor-associated antigen to decide T-cell killing specificity, and the other CAR can transmit a second T-cell-activation related signal by combing a ligand of a membrane receptor (such as EGFR (epidermal growth factor receptor) family protein) widely expressed by the tumor cells to promote T cell activation, proliferation and survival. The dual-signal independent chimeric antigen receptors (dsCAR) can avoid the potential safety problems on the basis of maintaining curative effects of second generation and third generation CAR.

The invention provides a method for detecting toxicity of antibiotic-type substances by utilizing luminous bacteria. The method comprises the following step of: prolonging the action time of antibiotic-type substances and the luminous bacteria to12-36 hours, preferably 24 hours. In the technical scheme provided by the invention, the action time of the antibiotic-type substances and the luminous bacteria is prolonged, so that the proliferation of the luminous bacteria during the period is ensured; and through the method, good timeliness of the accurate characterization of the biotoxicity of antibiotic can be realized, the detection result is more accurate on the basis of guaranteeing the timeliness, and the detection of toxicity delay of antibiotic-type pollutants is realized.

The invention discloses a long-term multiplication and storage method for embryonic callus of sweet potatoes. The method comprises the processes of selection of successive embryonic callus, successive multiplication of the embryonic callus, and induction of the embryonic callus to mature embryo regeneration. The method has the advantages that: the process flow is simple, the multiplication rate of the embryonic callus is high, and the successive time is long; the technical problem that the long-term utilization of the embryonic callus is limited because the materials are limited by seasons and the embryonic retaining time is short and the like in the sweet potato tissue culture process is solved; a good receptor material is provided for the transgene technology of the sweet potatoes; and a large amount of efficient regenerated material is provided for annually producing the tissue culture seedlings of the sweet potatoes.

The invention provides a method of manufacturing tissue engineering skin out of the body with epithelial stem cells. The method includes the following steps: (1) preparation of the epithelial stem cells: epithelial cells are made into cell suspension liquid, adhered and screened by IV collagen, and cultivated with epithelial stem cell media; (2) preparation of deepitheliarizing derma; (3) construction of the tissue engineering skin: epithelial stem cells for 2nd to 5th generation cultivation are selected, Dispase enzyme is adopted to digest the bossy cell patches in the size of rice to soya beam, the patches are inoculated to the deepitheliarizing derma, the tissue media is added, to cultivate the tissue engineering skin underwater or on the water. The tissue engineering skin constructed by the method of the invention is more suitable for the biological characteristics of human skin.

The invention relates to the field of biological medicine, and particularly provides an injection preparation of an anti-PD-L1 monoclonal antibody, which comprises the anti-PD-L1 monoclonal antibody with the protein content of 10-60 mg/ml; a buffer agent having a concentration of 10-60 mM; a stabilizer with the concentration of 100 to 150 mM; an osmotic pressure regulator with the concentration of10-100 mM; and 0.005%-0.05% (w/v) of a surfactant; wherein a pH value of the injection preparation is 5.8 to 6.3. The anti-PD-L1 monoclonal antibody disclosed by the invention has relatively high affinity and good biological activity; meanwhile, different auxiliary materials are selected according to the characteristics of the anti-PD-L1 monoclonal antibody, so that the safety and effectiveness of the monoclonal antibody in the preservation process can be guaranteed, and the physical stability, chemical stability and biological stability of the anti-PD-L1 monoclonal antibody can be maintained.

The present invention discloses a skin care product with the function of preventing air pollution and aims at providing a skin care product which can prevent various pollutions in the air from damaging the skin and keep the skin health. According to the weight percentage, the skin care product consists of 4 percent to 16 percent of composite protective active component and 84 percent to 93 percent of regular cosmetic basal component. The composite protective active component consists of anti-pollution factor, dustproof factor and ultraviolet absorption resistant agent and contains 3 percent to 8 percent of the anti-pollution factor, 0.5 percent to 3 percent of the dustproof factor and 0.5 percent to 5 percent of the ultraviolet absorption resistant agent according to the weight percentage. The composite protective active component of the skin care product of the present invention can bring the coordinate effect-increasing action into play well to achieve the purposes of face beautification and skin care by reasonable coordination and the selection of the dosage. The present invention can insulate the ultraviolet and at the same time can prevent and reduce the damage towards the skin caused by air pollution, which resists various kinds of pollution sources in the air completely to avoid using various kinds of protection articles synchronously and is convenient for using.

The invention provides a composition for oocyte and skin adult stem cell culture and applications thereof. The composition can improve the in-vitro maturation rate of oocyte, cleavage rate of ovum, and rate that embryo grows into mulberry and blastocyst; and thus the growth from in-vitro fertilized ovum to embryo is greatly promoted. At the same time, the differentiation from skin adult stem cells to fibroblast is effectively prohibited by the composition, or the self renewing and regeneration of skin adult stem cells are maintained. The composition comprises cysteamine, leukemia inhibitory factor, and Rho kinase inhibitor Y27632.

The invention belongs to the technical field of biology, and particularly relates to endothelial cell culture solution. The endothelial cell culture solution comprises a basic culture medium DMEM (dulbecco modified eagle medium), human platelet lysate, L-glutamine, vitamin C, laminin 10, laminin 11, fibronectin, penicillin and streptomycin. The endothelial cell culture solution has the advantages that a pH (potential of hydrogen) value of the endothelial cell culture solution is 6.8-7.0, and the osmotic pressure of the endothelial cell culture solution is 310 mOsm/kg; endothelial cells cultured by the endothelial cell culture solution are high in adherence and proliferation speed, purity, migration capacity and lumen formation rate, invasion effects of the endothelial cells can be inhibited, and accordingly the endothelial cell culture solution is a perfect endothelial cell culture system.

The invention discloses an acipenser dabryanus spermatogonium culture solution and application thereof. According to the culture solution, a low-serum culture medium which does not have fetal calf serum, adopts various growth factors, nonessential amino acid, sodium pyruvate and other additives as substitutes and is assisted by a small amount of male acipenser dabryanus serum is provided so as to guarantee mass reproduction of spermatogonium and low reproduction rate of a spermatic cell; and the spermatic cell 13d which is cultured to the 13rd day are doped with 25mu M BrdU and is marked as a newly reproduced cell. 24h immunohistochemical experiments indicate that the ratio of germ cell marked cells in newly reproduced cells after BrdU is doped for 24 hours is 85.78 percent. According to the culture solution, the current problem that the acipenser dabryanus spermatogonium passage cannot be massively cultured can be solved, and an effective cell platform is provided to acipenser dabryanus reproductive development researches.

The invention discloses a multi-stage temperature-control ventilation production method of multi-microorganism high-temperature bacterial fragrant koji, and belongs to the technical field of wine brewing. The method comprises the following steps: separately culturing high-temperature pure bacterial liquids, carrying out two-stage amplification culture to obtain a multi-microorganism high-temperature bacterial liquid, then carrying out multi-stage temperature control continuous ventilation or intermittent ventilation culture, and carrying out drying to obtain the multi-microorganism high-temperature bacterial fragrant koji. Advanced multi-microorganism high-temperature bacteria are heated actively and quickly in a brand-new temperature control mode, microbial contamination is effectively avoided, meanwhile, the multi-stage gradient heating and ventilation mode ensures the temperature rise adaptability of effective microbial communities, and proliferation of bacteria and generation and accumulation of metabolic flavor substances are ensured; the produced multi-microorganism high-temperature bacterial fragrant koji is rich in Maotai-flavour and scorch aroma and typical in aroma, and can be used for producing soft and other types of liquor seasoning wines, and the economic benefit is improved.

The invention discloses a bionic three-dimensional DPCs independent co-culture system and a construction method thereof. The construction method comprises the following steps: preparing single-cell suspensions of DPCs, DSCs and HGMCs; providing Matrigel, adding the DPCs single-cell suspension into the Matrigel, and carrying out resuspension to form a Matrigel-DP cell suspension; enabling the Matrigel-DP cell suspension to be adhered to a microporous membrane of a Transwell cell, so as to prepare a three-dimensional DPCs culture system; inoculating HGMCs in an upper chamber of the Transwell cell of the three-dimensional DPCs culture system, simultaneously inoculating DSCs in a lower chamber, and then adding a culture medium to prepare the bionic three-dimensional DPCs independent co-culturesystem. The in-vivo microenvironment of the DPCs can be effectively simulated, the difference between in-vitro cultured DPCs and in-vivo normal DPCs is reduced, and in-vitro long-time maintenance ofbiological functions of the DPCs is realized.

The invention discloses a brewing method of originally brewed low-alcohol raspberry beer. According to the brewing method of the raspberry beer, provided by the invention, raw materials including raspberry juice and original wheat juice are adopted, and the beer is brewed by fermenting the raw materials by using Leid yeast; the alcoholic strength of the raspberry beer is smaller than or equal to 2.5% vol, the sugar content of the raspberry juice is larger than or equal to 300 g/L, and the sugar content of the original wort is 6.0 +/-0.5 degree P. According to the method, the alcoholic strength of the raspberry beer can be effectively controlled, the sour-sweet balance degree of the finished wine is adjusted, and the foam retention of the product is guaranteed. 100L large-scale production verifies that the raspberry beer obtained by the invention has the alcoholic strength of less than or equal to 2.5% vol, and is clear and transparent in appearance, rich in raspberry fragrance, moderate in taste, harmonious in mouth, sour and refreshing in mouth closing, free of other foreign flavors and suitable for being eaten together with rice or bread.

The invention discloses a brewing method for brewing originally brewed low-alcohol blackcurrant beer. According to the method for brewing the blackcurrant beer, blackcurrant juice and mixed wort are used as raw materials, and the beer is brewed by fermenting the blackcurrant juice and the mixed wort by using saccharomyces. The alcoholic strength of the blackcurrant beer is smaller than or equal to 2.5% vol, the sugar content of the blackcurrant juice is larger than or equal to 300 g/L, and the sugar content of the barley wort is 5.5-6.5 DEG P. The blackcurrant beer brewed by the method disclosed by the invention is standard purple black in appearance, strong in blackcurrant fragrance, strong in taste, harmonious in mouth feel, moderate in alcohol warm feeling, typical in blackcurrant herbal fragrance, free of other foreign flavors and suitable for being eaten together with rice or bread.

The invention belongs to the field of culture mediums, and particularly relates to a serum-free chemical component definition culture medium for EB66 cell strain suspension growth. The culture mediumcomprises an additive A including, according to the concentrations of the substances in the culture medium, 1-10 mg/L of insulin, 5-10 microgram/L of IGF-1, 0.5-2 mg of putrescine, 0.5-5 mg/L of glutathione, 1-5 mg/L of neovaricaine, 10-50 micromole of beta-mercaptoethanol, 1-5 mg/L of lecithin, 10-50 mg/L of vitamin C, 50-100 mg/L of taurine, 2-5 mg/L of cholesterol and 3-6 millimole of glutamine, wherein 1000-3000 mg of an amino acid mixed component is added to per liter of the culture medium. The culture medium supports rapid proliferation of EB66 cells under the serum-free and full-suspension conditions, and the doubling time is short. The cells are high in density. Besides, the invention further provides a preparation method of the culture medium.

The invention provides a nitrogen and phosphorus treatment drainage channel structure for agricultural non-point source pollution treatment, and relates to the field of drainage channels. According to the nitrogen and phosphorus treatment drainage channel structure for agricultural non-point source pollution treatment, the nitrogen and phosphorus treatment drainage channel structure comprises a first section and a second section, a filter box is arranged between the first section and the second section, a prefabricated bottom plate is laid at the bottom of the nitrogen and phosphorus treatment drainage channel structure, and cement baffles are arranged on the two sides of the prefabricated bottom plate; grass planting bricks are laid on side slopes on the two sides of the drainage channel structure for nitrogen and phosphorus treatment, a first porous medium is laid between the two cement baffles in the first section, cobblestones are laid on the first porous medium, ceramsite is further laid on the second section on the basis of the first section, and the first porous medium and the second porous medium are laid on the basis of the second section. Second porous media are laid on the lower portion of the side slope of the drainage channel structure for nitrogen and phosphorus treatment and the two sides of the cobblestones. By adopting the modes of plant planting and microbiological treatment, the water channel has the purification capacity.