108results about How to "Determination method is simple" patented technology

The invention relates to an agent which can test the fiber proteinogen content without diluting plasma sample, and a relative test method, wherein the agent comprises 2-20 units of thrombase in each milliliter agent, 5-100 mill mol buffer agents in each liter agent, 0. 05-10g in each 100g milliliter agent, and the left is deionized water. And the test method comprises that (1), using the buffer liquid with stabilizer to prepare the agent which contains 2-20 units of thrombase in each milliliter, (2), a, cultivating the agent and the plasma sample at 37Deg. C, to be mixed in the volume ratio as 1:1, b, recording the blood coagulation time, c, using a standard curvature to obtain the fiber proteinogen content of the sample. The invention can avoid diluting sample in test, with simple test method, reduced agent production cost, improved test accuracy and reduced operation error.

The invention provides a detection method for a fingerprint chromatogram of flavonoid and organic acid components in a ginkgo biloba extract. The detection method comprises the following steps: (1) preparing a test solution; (2) preparing a reference solution; (3) respectively determining the test solution and the reference solution by adopting high-performance liquid chromatography, and comparing the acquired fingerprint chromatogram with a standard fingerprint chromatogram to obtain the fingerprint chromatogram of the flavonoid and organic acid components in the test solution. The invention further provides the application of the detection method in the quality detection and the component test determination of the flavonoid and organic acid components in the ginkgo biloba extract. According to the detection method for the fingerprint chromatogram of flavonoid and organic acid components in the ginkgo biloba extract and the application of the detection method provided by the invention, the fingerprint chromatogram of flavonoid and organic acid medicinal components in the ginkgo biloba extract is established; the quality control level of the flavonoid and organic acid medicinal components in the ginkgo biloba extract is improved; effective quantitative analysis can be performed.

The invention discloses a method for determining thermal-conduction resistance of a metal foam porous medium. The method comprises the following steps: placing a to-be-determined metal foam porous medium between a hot plate and a cold plate and carrying out vacuumization; energizing and heating the hot plate at an upper part and cooling the cold plate at a lower part via a cooling module; when the whole heat transfer process reaches a stable state and a certain degree of vacuum is reached and maintained unchanged in a test section, acquiring data like temperature, current and voltage in real time by using a data acquisition and processing system; and processing the acquired data by using the data acquisition and processing system so as to eventually obtain thermal-conduction resistance of the metal foam porous medium and providing a test report. The determination method provided by the invention is rapid and simple and has high accuracy.

The invention relates to a determining method and device of nicotine in oral smokeless tobacco products, and belongs to the technical field of novel tobacco detection. The method comprises the following steps of putting a piece of piled filtration paper into a dissolution pool (5), wetting by artificial saliva, weighing 0.5-2.0g of oral smokeless tobacco products, filling into the dissolution pool (5) which is filled up with the piece of filtration paper, placing a collection bottle (6) below the dissolution pool (5), regulating the height of a support (7), and closing a door of a constant-temperature box (4) for dissolution test; and analyzing a release liquid which is collected in real time quantitatively by a high performance liquid chromatograph. An in-vitro dynamic release device consists of a constant-temperature water bath pot (1), a solvent storage bottle (2), a constant-flow pump (3), the constant temperature box (4), the dissolution pool (5), the collection bottle (6) and the support (7). The method provided by the invention has the advantages that operation is simple, convenient and rapid, and selectivity is good; the device has the advantages of simple structure and time and labor conservation, and is convenient to dismount and clean.

The invention relates to a method for analyzing and detecting gold elements in smelting materials, which comprises the following steps: a sample is added into nitric acid or tartaric acid for heating pretreatment; the test solution is filtered by filter paper, and residue is fully transferred to the filter paper and is washed; the filter paper and the residue are put in a ceramic crucible and are put in a muffle furnace at a temperature of 750 DEG C for full incineration; fresh aqua fortis is added for dissolving and incinerating the residue at a low temperature; a combustion supporting ratio of 5:1, a slit of 0.2mm, a lamp current of 2mA and a negative high voltage of 300V are chosen, an atomic absorption spectrophotometer is used for determining the light absorption values of the gold elements, and results are calculated and analyzed. The method of the invention for analyzing the gold elements in the smelting materials has simplicity, convenience, easy implementation, short flow, small investment, high determination accuracy and good repeatability.

The invention discloses a method for determination of phytic acid content of cotton seed powder. The method comprises collecting different kinds of cottonseed samples planted in different regions, carrying out sample husking, grinding, sieving and moisture balance on the samples, collecting full spectrum data, pretreating the near infrared spectroscopy data through a plurality of pretreatment methods, accurately determining sample phytic acid content through high performance ion chromatography, constructing the optimal PLS model in a full spectrum range through a full cross-validated method, carrying out variable selection on the spectroscopic data, building multiple correction models through a multivariate calibration regression method, building a near infrared spectroscopy correction model and detecting phytic acid content of cotton seed powder through the model. The method utilizes a Buchi NIR Flex-N500 Fourier transform near infrared spectrometer to acquire a spectrogram of cotton seed powder, has a fast determination speed and high accuracy, is environmentally friendly, convenient and efficient and has an important meaning for cultivation of low-phytic acid cotton and promotion of cotton side product processing and utilization.

The invention provides a method for detecting toxicity of antibiotic-type substances by utilizing luminous bacteria. The method comprises the following step of: prolonging the action time of antibiotic-type substances and the luminous bacteria to12-36 hours, preferably 24 hours. In the technical scheme provided by the invention, the action time of the antibiotic-type substances and the luminous bacteria is prolonged, so that the proliferation of the luminous bacteria during the period is ensured; and through the method, good timeliness of the accurate characterization of the biotoxicity of antibiotic can be realized, the detection result is more accurate on the basis of guaranteeing the timeliness, and the detection of toxicity delay of antibiotic-type pollutants is realized.

The invention discloses an online trapping and analyzing method for benzo[a]pyrene in thermal cracking products, and an apparatus thereof, and belongs to the technical field of analytic detection. The method comprises the following steps: filling a sample into a thermal cracking chamber, carrying out aerobic thermal cracking under a programmed heating condition, directly introducing the thermal cracking products to the cold injection port of a gas chromatograph through a transmission pipeline, and trapping through refrigerating by liquid nitrogen; and carrying out programmed heating on the cold injection port after finishing the thermal cracking, allowing the thermal cracking products to be gasified and to enter a gas chromatograph-mass spectrometer, and quantitatively analyzing the benzo[a]pyrene in the thermal cracking products through adopting a selected ion mode. The apparatus comprises helium (1), a nitrogen-oxygen gas mixture (2), a triple valve (3), a flow controller (4), a flow controller (5), a handle (6), a thermal cracker (7), the transmission pipeline (8), a nut (9), an injection isolation pad (10), the cold injection port (11) and the gas chromatograph-mass spectrometer (12). According to the invention, the method has the advantages of good reappearance, high recovery rate, simple operation, and experiment error reduction; and the apparatus has a simple structure.

The invention discloses an immunodetection kit for detecting adulterated cow milk in camel milk and an application thereof, wherein alpha-lactalbumin and alphas1-casein in camel milk are used as characteristic protein of the camel milk, and beta-lactoglobulin and kappa casein in cow milk are used as characteristic protein of the cow milk. After the camel milk characteristic protein is separated and purified, the polyclonal antibody and the monoclonal antibody prepared by the commercialized cow milk characteristic protein and the self-made camel milk characteristic protein can be applied to ELISA detection. The kit mainly adopts an indirect ELISA method to qualitatively or quantitatively detect the content of camel-derived alpha-lactalbumin, alphas1-casein or bovine-derived beta-lactoglobulin and kappa-casein in camel milk, the sample pretreatment process is simple, and after a camel milk sample is repeatedly centrifuged to obtain a milk sample, the content can be directly measured after diluted by diluent, and a large number of samples can be simultaneously detected. The determination method is accurate, rapid and convenient, and has wide practicability and development value in thefield of camel milk adulteration monitoring.

The invention relates to an analysis and detection method of gold element in a smelting material. The method comprises the following steps: adding samples to nitric acid and tartaric acid to be pre-heated; filtering the filtrate by filter paper and transferring residues completely to the filter paper and washing; placing the filter paper and the residues to a porcelain crucible and placing in a muffle furnace to ash completely; adding fresh aqua regia to dissolve ash at low temperature; selecting the fuel / assistant ratio of 5:1, slit: 0.2mm, lamp current: 2mA and negative high voltage: 300V, measuring the light absorption value of gold element by an atomic absorption spectrometer, and calculating and analyzing the result. The analytic method to the gold element in the smelting material is simple and feasible, short in flow, low in investment, high in measuring accuracy and good in reproducibility.

The invention discloses a method for detecting carbamate pesticide content in total particle matter in cigarette mainstream smoke, belonging to the technical field of tobacco chemical analysis. The method comprises the steps of putting a Cambridge filter with trapped total particle matter in cigarette mainstream smoke into a conical flask with a plug; adding 100ml of mixed solvent of ethyl acetate and cyclohexane according to the volume ratio of 85:15; putting the conical flask on an ultrasonic generator to extract for 30min; concentrating the extracted liquid to 0.5ml after drying; using the mixed solution of toluene and acetonitrile (1:3) to reach a volume of 2ml to be purified; putting Envi-carb / NH2SPE small column on a solid phase extracting device, adding 2g of anhydrous sodium sulfate to the Envi-carb / NH2 composite column; using 5ml of mixed solution of toluene and acetonitrile according to the volume ratio of 3:1 to perform activation; transferring the to-be-purified sample test liquid to the composite column, using 5ml of mixed solvent of acetonitrile and toluene according to the volume ratio of 3:1 to perform elution for three times, wherein the rate is not more than 5.0ml / min; concentrating the eluate to 0.5ml, using 5ml of dichloromethane to exchange solvents for two times, concentrating the exchanged test liquid to 1ml, and then performing analysis. The invention has the advantages of simple and convenient method and accurate detected data of pesticide content.

The invention provides a method for measuring the water content of graded broken stone and belongs to the field of graded broken stone materials and construction parameter testing. The method includes: using the mass proportion, accounting for the to-be-measured graded broken stone, of each broken sample as weight to calculate the weighted average of the water content of different drying broken samples, and using the calculation result as the water content of the to-be-measured graded broken stone. The method has the advantages that the defects that due to the fact that the graded broken stone is high in porosity, free of cohesiveness and poor in water retention capacity, water migrates, water content measuring by sampling a central point is not representative, coarse aggregate or fine aggregate is high in proportion during graded broken stone sampling, and the measured water content cannot truly reflect the water content of compaction test samples due to little sampling or sampling unevenness degree, and the compaction test result has a multi-camel-back curve are overcome, the accuracy and reliability of the measuring result are increased, and the method is simple and fast.

The invention discloses an instrument including an upper insert panel (10), a lower insert panel (11), a container (6), a fixed pulley (8), a connection belt, a dripping device, a controller (3), a baffle plate (7) and a pressure sensor (9). The invention also provides an application of the instrument in determination of adhesive force and a method for determination of the adhesive force. The standardized instrument for determination of the adhesive force is automatic to operate and rapid in response, can precisely control the experiment temperature, is quite suitable for determination of the adhesive force, and not only avoids the error generated by manual operation, but also makes different test data have comparability; and the determination method is simple, is accurate in results, and is quite beneficial for application in industries.

The invention discloses a method using a single point adsorption method to measure the specific surface area of atmospheric particulates. The single point adsorption method utilizes a methylene blue water solution and comprises the following steps: (1) collecting atmospheric particulates, cutting, and weighing; (2) preparing a diluted original solution of methylene blue and a standard solution, determining the absorbance of standard solution, and drawing a work curve; (3) determining the absorbance of methylene blue solution, and calculating the adsorption amount of methylene blue according to the work curve; (4) calculating the specific surface area of atmospheric particulates. The method is simple, feasible, fast, and convenient, is suitable for measuring the surface area of atmospheric particulates, and has the characteristics of simple technology, low cost, strong operability, and low instrument requirements.

The invention relates to a method for extracting soil calcium by using a strontium chloride solution, aiming at solving the technical problem that the traditional for extracting and measuring the soil calcium is fussy in steps and time-consuming. The method comprises the following steps of: preparing a 0.02mol / L strontium chloride solution first; then taking 2.00g of air-dried soil screened with a 2mm screen, and oscillating the air-dried soil for 30min (180r / min, 25-30 DEG C) on a gas bath oscillator by using 50ml strontium chloride solution, filtering, and diluting filtrate by using the strontium chloride solution to prepare a solution to be tested; and dissolving 2.497g of calcium carbonate with little amount of 1: 1 hydrochloric acid, boiling to remove carbon dioxide, carrying out constant-volume operation by using the strontium chloride solution, and preparing a calcium standard series solution by using the strontium chloride. The calcium in the solution to be tested and the calcium standard series solution are measured by using a flame atomic absorption spectrometry. Compared with the prior art, the method disclosed by the invention is free of centrifugation, simple and convenient and suitable for rapid measurement of a large amount of samples; and a releasing agent is not needed to be added when the calcium is measured by the atomic absorption spectrometry.

The invention discloses a coal bed roadside pressure relief zone width measuring method based on drill hole gas leakage, and prepares a coal bed roadside pressure relief zone width measuring system based on drill hole gas leakage first. The measuring system comprises a seepage gas pipeline, a connecting bar and a hole sealing capsule on the connecting bar, wherein one end of the seepage gas pipeline is connected with a high-pressure gas source, and a difunctional flowmeter is arranged on the seepage gas pipeline. The coal bed roadside pressure relief zone width measuring method based on the drill hole gas leakage is based on the system and realized through measuring the drill hole gas leakage per unit length. According to the system and the method, gas leakage in different depths of gas seepage chambers per unit length is measured to determine the roadside pressure relief zone width after a coal bed roadway is excavated; and the measuring technology and method are provided for determining the hole sealing depth for a roadside gas drainage drill hole, determining the range of a coal face for implementing an outburst prevention measure in an inclined direction, and improving the forecast accuracy of gas emission quality of an excavation working surface.

The invention discloses a method for determining an appropriate intercropping distance between crops and fruit trees of a fruit tree and crop composite system. The method is characterized in that the overground shade of the fruit tree and the underground competition of the crop and the fruit tree are considered as two independent parts, a crown width of a X-year fruit tree at the fruit tree and crop appropriate intercropping distance obtained by only considering the overground shade factor is set to L1, the fruit tree and crop appropriate intercropping distance obtained by only considering the underground competition factor is set to L2, the obtained final appropriate intercropping distance between the X-year fruit tree and the crop is set to L, and the L is equal to max{L1, L2}. The overground shade of the fruit tree takes the vertical projection area of the crown width of the fruit tree as a circle, the vertical protection area of the crown width of the fruit tree is used as the inappropriate crop planting area when the overground shade is considered, the underground competition of the crop and the fruit tree takes the horizontal distribution of fruit tree fine roots and the distribution of a crop root system as a circle, and the overlapping condition of the distribution area of the fruit tree fine roots and the distribution area of the crop root system cannot occur. The method has the advantage that the crown width of the fruit tree and the horizontal radius of the fine root growth change along with the age of the tree, and the characteristics of the crop root system are combined to determine the appropriate intercropping distance between the crop and the fruit tree of the fruit tree and crop composite system.

The invention belongs to the field of production of an automobile hub bearing unit and especially relates to a drive hub bearing unit spin riveting pressure measurement system and measurement method. The measurement system comprises an outer ring, an external steel ball, an internal steel ball, a flange plate, an external holder, an internal holder, a bolt and foil gauges. The system is reasonable in structure design and simple in measurement method, and can accurately measure axial pressure remaining on the hub bearing unit after the hub bearing unit finishes spin riveting process to determine axial pre-tightening effect under a bearing loading state, thereby preventing the problem of flange shaft rupture due to inner ring loosening and skid, and ensuring driving safety. The measurement can check reasonability of structure design of a spin riveting structure and select reasonable process parameters.

The invention discloses an in-serum sodium ion enzyme method determining reagent and method, finding a relatively good concentration by applying different reagent proportions on complexing ability of crown ether which has characters of cavity ether and sodium ions, and it is the same as the cavity ether in the aspects of sensitivity, accuracy, and precision. And the invention has determining effect similar to the determining effect obtained by the method adopting cavity ether, but the invention is simple and convenient and largely reduces cost.

The invention discloses a method for determining titer of a swine flu inactivated vaccine. The method comprises the following steps: immunizing a pig with a swine flue inactivated vaccine, collecting blood 21-28 days after immunization and separating blood serum; removing non-specific components in the serum to obtain a serum to be tested; diluting the swine flue inactivated antigen, and preparing a unit swine flue antigen diluent with a concentration of 4HA; and diluting the serum to be tested by multiple proportions, successively adding the 4HA unit swine flue antigen diluent and 1% red cells to conduct hemagglutination inhibition test, so as to completely inhibit highest dilution of the 4HA unit swine flu antigen serum at HI titer. The method has advantages of greatly shortened measurement time, little operation error, strong controllability, and small inter-batch difference, and also reduces detection errors caused by different levels of experimental animals in an animal challenge protection experiment for testing the titer.

The invention relates to a method for determining the pollen number in a rice anther. The method is characterized by: making 168 grids with a size of 2280 micrometers*3048 micrometers by metal cautery lines at the center of a reticle; dripping 1-2 drops of an I2-KI solution with a concentration of 0.1%-0.3% on the reticle, squeezing an anther by a tweezer repeatedly at different places in the I2-KI solution till the pollen in the anther is dispersed completely, then adding 1-2 drops of an I2-KI solution with a concentration of 1% into the original solution to dye the pollen into a deep color; distributing the pollen sample in the grids of the reticle uniformly; photographing the grids in the reticle completely one by one in order to record them by a digital microscope; and employing image analysis software to carry out differential counting on the photographed pollen in terms of active pollen, inactive pollen and total pollen. The method provided in the invention can accurately determine the number of active pollen and inactive pollen in the anther, and has the characteristics of convenient determination, reliable data, and high determination efficiency.

The invention discloses a determination method for the chromium content in cotton-seed meal. The determination method includes the steps that different kinds of cotton-seed meal samples planted in different regions are prepared, a near-infrared spectrometer is used for collecting spectrums of the cotton-seed meal samples, full cross validation is adopted in a full spectrum range to build a PLS model, and then the PLS model is analyzed by adopting eight preprocessing methods; a processing method corresponding to the maximum prediction correlation coefficient R2 and the minimum root-mean-square error RMSE is used as the optimal preprocessing method, and a near-infrared spectrum correction model is set up through the optimal preprocessing method; the near-infrared spectrum correction model built in the step mentioned above is used for detecting the cotton-seed meal to be detected to obtain the chromium content of the cotton-seed meal. The FOSS5000 near-infrared spectrometer is used for collecting spectrograms of the cotton-seed meal, determination accuracy is high, and the determination method is fast, convenient and efficient and has great significance in cultivating new cotton species and promoting processing and utilization of cotton by-products.

The invention relates to a quick measuring method of pyrethrin, which can quickly and effectively measure the content of the pyrethrin in dried flowers. The method comprises the following steps of: picking up 10-20 capitulums when tubular pyrethrum flowers bloom by 50-99 percent, and placing the capitulums into an oven at 40-80 DEG C for drying for 36-72 hours; pulverizing, then weighing 50-200mg of powder and putting the weighed powder into a glass centrifuge tube; adding 2-10mL of normal hexane, covering the glass centrifuge tube and then oscillating for 20-40s on a vortex mixer; placing the glass centrifuge tube in an ultrasonic water bath for 5-15min, taking out and then continuing to oscillating for 20-40s; centrifuging for 3-8min at a speed of 1,500-3,000 turns / min at room temperature of 20-25 DEG C; absorbing 0.1-0.5mL supernate, and metered by the volume, diluting for 40-80 times with absolute ethyl alcohol as a solution to be measured; measuring the light absorbing valve by an ultraviolet and visible spectrophotometer under the condition of a wavelength of 225nm; and calculating the content of the pyrethrin. The invention has the advantages of simple pretreatment, low testing cost, high speed and good accuracy and can improve the field breeding measuring efficiency and the selecting efficiency of good genotypes.

The invention discloses a device and a method for measuring gas micro-flow under experimental reservoir conditions. The device comprises: a sapphire tube, an inlet end joint which is connected to oneend of the sapphire tube and comprises a gas inlet of an inlet end and a liquid inlet of the inlet end, an outlet end joint which is connected to the other end of the sapphire tube and comprises the gas inlet of an outlet end, a first injection pump which is arranged at the inlet end, a liquid intermediate container which is arranged between the first injection pump and the liquid inlet of the inlet end, a second injection pump which is arranged at the outlet end, and a gas pressure container which is arranged between the second injection pump and the gas inlet of the outlet end. The device has advantages of being simple and convenient in operation method and relatively high in test result precision.

The invention provides a finger-print detection method for a Weikening tablet. The method can obtain a finger print of the Weikening tablet by preparing, derivatizing and measuring test solution. The invention further provides a finger-print detection method for amino acid ingredients in the Weikening tablet. The finger-print detection method for amino acid ingredients in the Weikening tablet can obtain liquid chromatograms of the test solution, comparison product solution and blank contrast solution by preparing, derivatizing and measuring the test solution, the comparison product solution and the blank contrast solution, and the liquid chromatogram of the test solution is compared with liquid chromatograms of the comparison product solution and the blank contrast solution to obtain finger prints of the amino acid ingredients. The method also can serve as a measuring method for the content of the amino acid ingredients in the Weikening tablet and a quality detection method of the Weikening tablet. According to the method disclosed by the invention, measured results of the amino acid ingredients in the Weikening tablet are reliable; accuracy, repeatability and stability are good; the method also can serve as a quality control method of the Weikening tablet; multi-ingredient quantitative control on the quality of the Weikening tablet is achieved; the quality control level of the Weikening tablet is improved.

The invention discloses application of rice quality indexes in identification of main cropping rice and ratooning cropping rice of ratoon rice. Quality indexes in main cropping rice and ratooning cropping rice of ratoon rice are detected by virtue of the conventional means at present so as to determine the main cropping rice and the ratooning cropping rice. The percentage of chalky rice, chalkiness degrees, amylose content and alkali spreading values in grains of the ratooning cropping rice have obvious differences from index values in grains of the main cropping rice, the index values of thepercentage of chalky rice and the chalkiness degrees are obviously lower than those in the main cropping rice, and the index values of the amylose content and the alkali spreading values are obviouslyhigher than those in the main cropping rice. According to the application disclosed by the invention, the main cropping rice and ratooning cropping rice of the ratoon rice can be accurately identified, the determination method is simple and convenient, and the determination cost is low. The technical measures can be widely applied to identification of the main cropping rice and the ratooning cropping rice of the ratoon rice in different ecological zones of rice cultivation.

The invention discloses a quantitative measuring method for corrosion degree of a plate grid. The quantitative measuring method comprises the following steps of firstly, sampling from to-be-detected pole plates, and selecting a rectangular sample from the part with the grid plate, wherein the length direction of the plate grid is the same as the length direction of the sample; soaking the sample into pure water, fetching out, sending into an oven to dry, cooling the sample to the room temperature, coating epoxy resin to the outer layer, completely curing the epoxy resin, and cutting the sample into slices, wherein the cutting direction is vertical to the length direction of the plate grid; putting the sample slice into an EDS(energy disperse spectroscopy), scanning by EDS lines, setting the scanning initial point at the center point of the cross section of the sample slice, horizontally scanning towards one side, and calculating the thickness size of the corrosion layer of the outer surface of the plate grid and the content of residual alloy component according to the scanning peak area recorded by the EDS. The quantitative measuring method has the advantage that by performing quantitative measuring, a research and development person can precisely evaluate the service life of the plate grid part of the pole plate of a lead acid storage battery, so that the targeted improvement design can be performed.

The invention belongs to the field of chemistry and is suitable for measuring four elements including nitrogen, hydrogen, carbon and sulfur in tree moss concrete. The invention relates to a method for measuring nitrogen, hydrogen, carbon and sulfur in tree moss concrete, which comprises the following steps of: 1, quantitatively weighing tree moss concrete samples, and adding the same into a special sample cup; 2, quantitatively introducing a carrier gas (helium 0.14MPa), a power gas (argon 0.4PMPa) and a burning gas (oxygen 0.1MPa) in per 1 gram of tree moss concrete; 3, cracking the tree moss concrete at high temperature, burning in pure oxygen, and converting nitrogen, hydrogen, carbon and sulfur in the samples into gases including N2, H2O, CO2 and SO2; and 4, separating N2, H2O, CO2 and SO2 through chromatographic columns, and carrying out thermal conductivity detection on the percentages of nitrogen, hydrogen, carbon and sulfur.

The invention discloses a microlens imaging-based antigen antibody reaction determination method. According to the microlens imaging-based antigen antibody reaction determination method, a microlens is immersed in a sample solution containing antigens, and under parallel light irradiation, an image is obtained, wherein the obtained image is a bright circle with a dark peripheral edge ring; an antibody solution is added dropwise, solution refractive index is changed after antigen antibody reaction, so that the thickness of the microlens image dark ring is changed; the thickness changing is determined, a formula derived from geometric optical principles is used for calculating the change of the solution refractive index after antigen antibody reaction, a curve used for representing the relationship of the changing amount with the concentration of the antigen is constructed, and it can be qualitatively determined whether antigen antibody reaction is induced, and antigen concentration and reaction dynamic parameters can be obtained via quantitative determination. Operation of the microlens imaging-based antigen antibody reaction determination method is simple and rapid; the microlens imaging-based antigen antibody reaction determination method can be used for realizing qualitative and quantitative determination of antigen antibody reaction; coating of a solid carrier with the antigen or the antibody before detection is not needed; and washing after detection is not needed.

The invention discloses a rapid determination method of the content of soluble proteins and relates to the field of detection of the content of proteins. The invention provides a mathematical model for the content of the soluble proteins and an alkali adding amount in an ultrasonic-assisted protein extraction process and the model can be used for predicating the content of the soluble proteins at different time. The rapid determination method disclosed by the invention has the advantages that the established mathematical model has a very good linear relation and the alkali adding amount in a reaction process can be obtained online; a determination result of the proteins is accurate and reliable; compared with an offline determination method, the rapid determination method is simpler and more accurate and the aim of monitoring production in real time can be realized.