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Method for determining titer of swine flu inactivated vaccine

An inactivated vaccine and potency determination technology, which is applied in the field of veterinary biological products, can solve the problems of respiratory natural barrier damage, poor controllability, economic loss, etc., and achieve the effects of shortening the measurement time, simple and convenient measurement method, and small operational errors

Active Publication Date: 2012-10-17
GUANGZHOU SOUTH CHINA BIOLOGICAL MEDICINE +1
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  • Abstract
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AI Technical Summary

Problems solved by technology

SI not only reduces the production performance of affected pigs, reduces the meat-to-feed ratio, directly affects the health of the pig herd, and causes pigs to die, but also swine influenza virus has a high affinity for pig respiratory epithelial cells, causing the respiratory tract of pigs to naturally The barrier is destroyed, leading to concurrent or secondary infection of various bacteria or viruses such as porcine respiratory and reproductive syndrome virus, porcine respiratory coronavirus, porcine pleuropneumoniae, etc., making the epidemic more complicated and aggravated
In addition, SI is one of the main immunosuppressive diseases of pigs, which can cause huge waste of labor, feed and medicine, resulting in huge economic losses
[0003] Inoculation with inactivated vaccine is an effective way to control swine flu. However, there is no commercialized inactivated swine flu vaccine in China at present. Determining the efficacy test method of vaccine is the key technology for vaccine development.
At present, the method used by domestic research institutes to test the efficacy of vaccines is the challenge protection test. This method takes a long time and is poor in controllability. The evaluation results are often due to the different grades of animals used, and the test results often have errors.
[0004] The inability to quickly and accurately determine the potency of swine flu vaccines has become a difficulty in the development of swine flu vaccines

Method used

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  • Method for determining titer of swine flu inactivated vaccine
  • Method for determining titer of swine flu inactivated vaccine

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Embodiment Construction

[0025] Below in conjunction with embodiment, further illustrate the present invention.

[0026] Obtaining and pretreatment of porcine serum:

[0027] 1) Five 35-day-old negative (HI subtype influenza HI antibody titer ≤ 1:20) susceptible piglets were injected with 3 ml of swine influenza inactivated vaccine (H1N1) at two points on the left and right sides of each neck muscle. Day, together with 5 control pigs, blood was collected and serum was separated;

[0028] 2) In order to remove the non-specific components in the serum, mix the serum with the receptor-destroying enzyme (RDE) at a volume ratio of 1:4, place it in a water bath at 37°C for 16-18 hours, and then transfer it to a water bath at 56°C for 30 minutes to inactivate it Residual RDE, take it out and cool it down;

[0029] 3) Add concentrated chicken red blood cells so that the final concentration is 5% to 20%, and adsorb at room temperature for 30 minutes to remove other non-specific components;

[0030] 4) The...

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Abstract

The invention discloses a method for determining titer of a swine flu inactivated vaccine. The method comprises the following steps: immunizing a pig with a swine flue inactivated vaccine, collecting blood 21-28 days after immunization and separating blood serum; removing non-specific components in the serum to obtain a serum to be tested; diluting the swine flue inactivated antigen, and preparing a unit swine flue antigen diluent with a concentration of 4HA; and diluting the serum to be tested by multiple proportions, successively adding the 4HA unit swine flue antigen diluent and 1% red cells to conduct hemagglutination inhibition test, so as to completely inhibit highest dilution of the 4HA unit swine flu antigen serum at HI titer. The method has advantages of greatly shortened measurement time, little operation error, strong controllability, and small inter-batch difference, and also reduces detection errors caused by different levels of experimental animals in an animal challenge protection experiment for testing the titer.

Description

technical field [0001] this invention one The invention discloses a method for testing the effectiveness of a vaccine, in particular to a method for measuring the potency of an inactivated swine influenza vaccine, belonging to the field of veterinary biological products. Background technique [0002] Swine influenza (Swine Influenza, SI) is an acute, highly contagious porcine respiratory infectious disease caused by influenza A virus of the family Orthomyxoviridae. Clinically the disease is characterized by sudden onset of cough, fever, dyspnea, prostration, rapid recovery, or death. The disease can occur in pigs of all ages and breeds, and the incidence rate can be as high as 100%. SI not only reduces the production performance of affected pigs, reduces the meat-to-feed ratio, directly affects the health of the pig herd, and causes pigs to die, but also swine influenza virus has a high affinity for pig respiratory epithelial cells, causing the respiratory tract of pigs to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/86
Inventor 叶贺佳仇微红梁昭平廖明罗开健王斌李敏孙招金
Owner GUANGZHOU SOUTH CHINA BIOLOGICAL MEDICINE
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