2results about How to "Small batch-to-batch variance" patented technology

This invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and its application. The culture medium contains inorganic salts, amino acids, vitamins, energy substances, buffers, antioxidants, sterols, polyamines, additives, and indicators. The antioxidants include dihydrolipoic acid (DLA) and S-acetyl-L-glutathione. This invention provides a culture medium with clearly defined components and significant culture effects for serum-free suspension culture of BHK-21 cells. The added DLA and S-acetyl-L-glutathione, as a combination of antioxidants in the culture medium, enhance the antioxidant properties of the serum-free suspension medium while also improving the cell viability of BHK-21 cells cultured in the medium.

This invention relates to the field of nanomaterials technology, specifically to a method for preparing silica using continuous flow. The method involves mixing an organic base, a surfactant, and water to obtain solution A; mixing a silicon source and an organic solvent to obtain solution B; reacting solutions A and B through a continuous flow apparatus, separating the solid product, and calcining it to obtain silica; wherein the flow rates of solutions A and B are 5-10 mL / min, and the reaction temperature is 20-45℃. In this method, silica is prepared using a continuous flow process, and the prepared silica has a nanosphere morphology with a specific surface area of ​​200-800 m². 2 / g, with a pore size distribution of 2-50 nm and a particle size distribution deviation (PDI) of less than 2%.