9results about How to "Small batch-to-batch variance" patented technology

The invention belongs to the field of molecular imaging probes for cancer diagnosis, and discloses a targeted nucleic acid aptamer aiming at tumor-associated calcium channel sensing protein 2, a molecular imaging probe for diagnosing lung cancer and a preparation method of the molecular imaging probe. The nucleic acid aptamer contains 46 nucleotides, can specifically target the surface Trop-2 of a lung cancer tumor cell, has a specific targeting recognition effect with Trop-2 overexpressed lung cancer cells such as non-small cell lung cancer cells H1650 and small cell lung cancer cells SBC-2, and can be used for molecular imaging of Trop-2 positive lung cancer patients. The molecular imaging probe can be used for noninvasive visual molecular imaging of lung cancer, and has the advantages of high specificity, high sensitivity, good imaging effect and the like. The preparation method of the molecular imaging probe is simple in reaction condition, low in cost and easy for clinical transformation.

The invention relates to the technical field of pharmaceutical preparations, and particularly discloses a preparation process of lidocaine hydrochloride gel. The preparation process of the lidocaine hydrochloride gel provided by the invention comprises the following steps: preparing materials, sterilizing and carrying out stepped cooling, the step-type cooling process comprises the following specific steps: in the first stage, firstly preserving heat at 90-60 DEG C for 1-2 hours, wherein the stirring rotating speed is 10-60 rpm; in the second stage, heat preservation is conducted for 1-2 h at the temperature of 60-40 DEG C, and the stirring rotating speed is 10-60 rpm; and a third stage: finally standing and preserving heat at 40-10 DEG C for 5-15 hours. According to the preparation process of the lidocaine hydrochloride gel provided by the invention, the colorless and transparent lidocaine hydrochloride gel with high uniformity and good stability can be obtained, and the practical reliability and safety of the lidocaine hydrochloride gel are greatly improved.

The invention provides a tandospirone citrate film-coated tablet and a preparation method thereof.The film-coated tablet comprises a tablet core and a coating, and the tablet core is prepared from tandospirone citrate, pregelatinized starch, corn starch, calcium carboxymethyl cellulose, polyvinyl alcohol EG-05P and magnesium stearate; according to the present invention, the coating powder comprising hydroxypropyl methylcellulose, concentrated glycerol, titanium dioxide and carnauba wax is adopted to coat the obtained tablet core, the use amount of each component and the coating weight gain ratio are controlled, the hygroscopicity of the tandospirone citrate is covered, and the fluidized bed granulation mode is adopted to substantially reduce the batch-to-batch difference, and simultaneously control the particle size of the bulk drug, according to the present invention, the dissolution rate of the sample can be always maintained within a certain range, and the dissolution RSD in the phosphate medium with the pH value of 6.8 for 5 min is far less than the dissolution RSD in the original development agent, such that the intra-batch uniformity is ensured, and the problems of non-uniform granulation, large inter-batch difference, and lactose intolerance due to the long-term use of lactose during the preparation process are solved.

This invention relates to the field of nanomaterials technology, specifically to a method for preparing silica using continuous flow. The method involves mixing an organic base, a surfactant, and water to obtain solution A; mixing a silicon source and an organic solvent to obtain solution B; reacting solutions A and B through a continuous flow apparatus, separating the solid product, and calcining it to obtain silica; wherein the flow rates of solutions A and B are 5-10 mL / min, and the reaction temperature is 20-45℃. In this method, silica is prepared using a continuous flow process, and the prepared silica has a nanosphere morphology with a specific surface area of ​​200-800 m². 2 / g, with a pore size distribution of 2-50 nm and a particle size distribution deviation (PDI) of less than 2%.

The invention relates to the technical field of medicine preparation, in particular to a preparation method of composite calcium bilirubin. The preparation method of the composite calcium bilirubin provided by the invention comprises the following steps: mixing calcium taurine, water, bilirubin and beta-glucuronidase, and carrying out catalytic reaction to obtain the composite calcium bilirubin, the mass ratio of the bilirubin to the calcium taurine is 1: 1.05; the mass of the water is 5 times of the sum of the mass of the bilirubin and the calcium taurine; the dosage ratio of the bilirubin to the beta-glucuronidase is 100g: 2ku; the temperature of the catalytic reaction is room temperature, and the time is 4 hours. According to the invention, the taurine calcium and the bilirubin are adopted as raw materials, the beta-glucuronidase is utilized to realize the preparation of the composite calcium bilirubin, and the process is optimized, so that the difference between batches of the product is smaller, and the quality is more stable. In addition, the method provided by the invention has simple steps and can be industrialized.

This invention discloses a lactation-promoting composition containing a lime flavonoid extract, comprising, by weight, the following raw material components: 10-50 parts lime flavonoid extract, 5-30 parts synergistic active ingredients, and 20-85 parts excipients; wherein the lime flavonoid extract contains ≥80% total flavonoids (calculated as hesperidin), of which hesperidin ≥40%, neohesperidin ≥15%, naringin ≥10%, and hesperidin ≥5%. Using the lime flavonoid extract as the core, it achieves highly efficient lactation promotion through multi-target synergistic effects. In livestock applications, it can increase milk yield in dairy cows by 15%-28% and milk yield in sows by 20%-38%, while significantly improving milk fat, milk protein, and other milk nutritional indicators.

The invention relates to the field of medical detection, provides a preparation method of a novel BCR / ABL fusion gene fluorescence in-situ hybridization probe, solves the problems that an existing common fluorescence in-situ hybridization probe is complex in preparation process and is limited by BAC cloning, and overcomes the problems that an existing probe is non-uniform in cell permeation, high in background and weak in signal intensity and is combined with non-specific fragments. The preparation method of the fluorescent probe provided by the invention is suitable for practical application, the fluorescent probe which is uniform in size, high in cell transmittance, uniform in penetration, high in targeting specificity, low in background, clear and obvious in signal, simple and efficient in production mode and capable of being synthesized at one time to meet permanent application requirements is prepared, and through process exploration, the fluorescent probe has a good application prospect. The dye, the dye concentration, the primer and the high-fidelity polymerase which are most suitable for the fluorescence labeling process of the BCR / ABL fusion gene fluorescence in-situ hybridization probe are screened out.

The utility model relates to the technical field of biological fermentation equipment, and discloses an efficient cellulosic ethanol synchronous saccharification and fermentation device which comprises a mixing bin, a cover plate is arranged at the top of the mixing bin, a first motor is fixedly connected in the cover plate, the output end of the first motor is fixedly connected with a straight gear, a fluted disc is rotationally connected in the cover plate, and a second motor is fixedly connected in the fluted disc. The fluted disc is meshed with the straight gear, a scraping plate is fixedly connected to one side of the outer wall of the fluted disc, the outer wall of the scraping plate is slidably connected to the inner wall of the mixing bin, and a first stirring rod is fixedly connected to the other side of the outer wall of the fluted disc. According to the utility model, firstly, the motor drives the straight gear to rotate so as to drive the fluted disc to rotate and drive the scraping plate to scrape materials on the inner wall of the mixing bin, so that the effect of preventing the materials from adhering to the wall is achieved, the problem that the quality of produced ethanol is not uniform due to insufficient stirring is solved, and the aims of fully stirring cellulase and fermentation liquor and improving the fermentation efficiency are achieved. Therefore, the ethanol production quality is stabilized.

This invention relates to a culture medium for high-density serum-free suspension culture of BHK-21 cells and its application. The culture medium contains inorganic salts, amino acids, vitamins, energy substances, buffers, antioxidants, sterols, polyamines, additives, and indicators. The antioxidants include dihydrolipoic acid (DLA) and S-acetyl-L-glutathione. This invention provides a culture medium with clearly defined components and significant culture effects for serum-free suspension culture of BHK-21 cells. The added DLA and S-acetyl-L-glutathione, as a combination of antioxidants in the culture medium, enhance the antioxidant properties of the serum-free suspension medium while also improving the cell viability of BHK-21 cells cultured in the medium.