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Detection kit and preparation method thereof for drugs of benzodiazepines

A detection kit and benzodiazepine technology are applied in the field of immunocolloidal gold rapid detection kits and their preparation, which can solve the problems of slow speed and complicated operation, and achieve the advantages of reduced dosage, sensitive color reaction and stable effect. Effect

Active Publication Date: 2012-07-11
SHANGHAI CHEMTRON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the shortcomings of the prior art, such as enzyme amplification enzyme immunoassay, fluorescence polarization immunoassay, radioimmunoassay and other assay methods to detect benzodiazepines slow speed, complicated operation, etc., to provide a fast, convenient, Sensitive benzodiazepine drug detection kit and its application independent of special equipment

Method used

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  • Detection kit and preparation method thereof for drugs of benzodiazepines
  • Detection kit and preparation method thereof for drugs of benzodiazepines
  • Detection kit and preparation method thereof for drugs of benzodiazepines

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] The preparation of the detection kit of embodiment 1 benzodiazepines

[0065] Reagent source:

[0066] Anti-benzodiazepine monoclonal antibody, goat anti-mouse IgG polyclonal antibody, benzodiazepine-bovine serum albumin complex (BZ0-BSA): purchased from MAXMED LABORATORIES INC.

[0067] Nitrocellulose film, glass fiber paper: purchased from Germany Sartorius (SARTORIUS)

[0068] Reagent preparation:

[0069] 0.1M pH7.6 phosphate buffer: weigh 10.2gNa 2 HPO 4 , 3.4gNaH 2 PO 4 , add 900ml of purified water to dissolve, adjust the pH value of the solution to 7.6 with hydrochloric acid, and dilute to 1000ml with purified water.

[0070] 10% bovine serum albumin (BSA) solution: Weigh 100 g of BSA, dissolve it in purified water, and dilute to 1000 ml.

[0071] 0.1% BSA borate buffer solution: Weigh 9.535g of borax and dissolve it in 900ml of purified water, adjust the pH value to 8.6±0.05 with hydrochloric acid, and set the volume to 1000ml to obtain 0.1M borate buffe...

Embodiment 2

[0109] The sensitivity experiment of the detection kit of embodiment 2 benzodiazepines

[0110] Detection method:

[0111] 1. Take the detection kit of benzodiazepines prepared in Example 1, and place the kit on a horizontal platform.

[0112] 2. Take the sample with the sample pipette, and then drop 3 drops (about 120ul) of the sample into the sample hole of the kit. Use a different pipette for each different sample.

[0113] 3. Observation results: Interpret the results 3-8 minutes after adding the sample.

[0114] Test sample:

[0115] Quality control reference substances with oxazepam concentrations of 150ng / ml, 300ng / ml, and 600ng / ml were prepared as samples, and each concentration of each kit was repeated 6 times.

[0116] Sensitivity test result table

[0117] ng / ml

[0118] Oxazepam concentration

[0119] The detection sensitivity results of benzodiazepines

[0120] ng / ml

[0121] Element

[0122] The results confirmed that the prepared det...

Embodiment 3

[0123] The specificity experiment of the detection kit of embodiment 3 benzodiazepines

[0124] Using the method of Example 2, the following drugs were detected to observe whether cross-reaction occurred.

[0125] cross test

[0126]

[0127]

[0128] The test results show that the above drugs will not affect the test results when the concentration is equal to 100 μg / ml.

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Abstract

The invention discloses a detection kit and a preparation method thereof for drugs of benzodiazepines. The detection kit provided by the invention includes a reagent strip, wherein the reagent strip includes sample filtering paper, immune colloid gold paper, an immune cellulose nitrate membrane and water absorbent paper which are arranged sequentially from the sample feeding end, wherein the immune colloid gold paper is a fiber glass gasket coated by an anti-benzodiazepine monoclonal antibody labeled by colloid gold; the immune cellulose nitrate membrane is a cellulose nitrate membrane provided with a detection line and a quality control line; the detection line is coated with a benzodiazepine-bovine serum albumin compound; and the quality control line is coated with goat-anti-mouse IgG. The invention further provides a preparation method of the detection kit for drugs of benzodiazepines. Through adopting the detection kit and the preparation method provided by the invention, the consumption of monoclonal antibodies can be reduced, and the manufacturing cost is saved.

Description

technical field [0001] The invention relates to an immune colloidal gold detection kit, in particular to an immune colloidal gold rapid detection kit related to benzodiazepines and its preparation. Background technique [0002] Benzodiazepines are a class of drugs widely used clinically, most of which are derivatives of 1,4-benzodiazepines, mainly including diazepam (stable), flurazepam (flurazepam), chlordiazepoxide, oxalazine Shazepam and triazolam have sedative-hypnotic, anti-anxiety, anti-convulsant, and muscle-relaxing effects. However, large doses of benzodiazepines can cause ataxia, acute poisoning can cause coma and respiratory depression, long-term use can cause physical and mental dependence, and the risk of dependence increases with the increase in dose and the duration of treatment. extended and increased. Once physical dependence occurs, immediate discontinuation can lead to withdrawal symptoms, including headache, myalgia, extreme anxiety, restlessness, excit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/558
Inventor 张国华
Owner SHANGHAI CHEMTRON BIOTECH
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