28 results about "Sensitivity test" patented technology

An interface circuit includes a reference voltage generation circuit to generate a reference voltage, a differential voltage signal generation circuit to convert send data input in sending data into a pair of differential voltage signals and output the pair of differential voltage signals based on the reference voltage generated by the reference voltage generation circuit, a receiver to convert a pair of differential voltage signals input in receiving data and output received data, and a receiver test circuit to perform a sensitivity test of the receiver, the receiver test circuit having a resistance circuit to generate a pair of differential voltage signals having a potential difference being necessary for the sensitivity test of the receiver.

The invention provides a contaminated site remediation technology scoring system which comprises a multi-dimensional evaluation index system construction module, a situational weight dynamic adjustment module, a remediation technology applicability scoring module, an uncertainty analysis and sensitivity test module, a scoring result visualization module, a technical scheme sorting and screening unit and the like. Through four innovations of a dynamic weight adaptation mechanism, a multi-model fusion scoring algorithm, a technology combination scheme evaluation module and uncertainty robust analysis, the problems of single dimension, weight solidification, difficulty in quantification of a synergistic effect and the like in pollution remediation technology evaluation are systematically solved; and an objective, efficient and transparent intelligent decision-making tool is provided for repairing a complex polluted site.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) primer designed by taking a BXAEP1 gene of Bursaphelenchus xylophilus as a target gene, CRISPR (Clustered An LAMP primer and CRISPR RNA are designed aiming at a BXAEP1 gene of the pine wood nematode as a target gene, a detection kit and a detection method for detecting the pine wood nematode based on an LAMP-CRISPR / Cas12a system are established, specificity and sensitivity tests find that the CRISPR RNA shown in SEQ ID No.8 has the best detection effect, the pine wood nematode can be specifically distinguished from pine wood nematode, and the sensitivity reaches 10 fg / L; the established detection method for detecting the pine wood nematodes based on the LAMP-CRISPR / Cas12a system has the advantages of good stability, strong practicability, high amplification efficiency and the like, and a sensitive, specific, rapid and visual detection means is provided for rapidly screening the pine wood nematodes on site.

The invention discloses an LAMP primer designed by taking a BXPC1 gene of bursaphelenchus xylophilus as a target gene, CRISPR RNA and application of the LAMP primer. The invention designs an LAMP (loop-mediated isothermal amplification) primer and CRISPR (clustered regularly interspaced short palindromic repeats) RNA (ribonucleic acid) by aiming at a BXPC1 gene of the pine wood nematodes as a target gene and establishes a detection kit and a detection method for detecting the pine wood nematodes based on an LAMP-CRISPR / Cas12a system. Specificity and sensitivity tests show that the CRISPR RNA as shown in SEQ ID No.8 has the best detection effect, pine wood nematode and bursaphelenchus mucronatus can be specifically distinguished, and the sensitivity reaches 0.1 pg / L; the method for detecting the pine wood nematodes based on the LAMP-CRISPR / Cas12a system has the advantages of being good in stability, high in practicability, high in amplification efficiency and the like, and a sensitive, specific, rapid and visual detection means is provided for rapidly screening the pine wood nematodes on site.

The invention discloses an antibacterial drug sensitivity test device which comprises a test bed, a vortex mixer, a support and a uniform spreading frame, a plurality of culture dishes are arranged on the test bed, alcohol lamps are arranged beside the culture dishes, a first test tube, a second test tube and a third test tube are arranged on the support, and the first test tube, the second test tube and the third test tube are arranged on the uniform spreading frame. Tweezers, a rubber head suction tube and an inoculation ring are sequentially arranged on the right side of the third test tube, and a motor is mounted on the outer side of the uniform spreading frame. A bacterial colony solution is injected into a culture dish, the culture dish is placed on a dish fixing seat, a bending column is rotated to enable a rotating disc to rotate, the culture dish is fixed by a rubber block, and the bacterial colony solution is uniformly spread in the culture dish through a uniform spreading frame, so that irregular growth of later experimental bacterial colonies caused by an ignored area is avoided; meanwhile, a comparison group and an antibiotic paper sheet group are arranged and a biofilm breaker group is added in the experiment, so that the accuracy of the experiment is enhanced, the hysteresis of the antibiotics is shown, and the inundation use of the antibiotics caused by errors of experimental data is avoided.

This invention discloses a primer set for multiplex PCR detection of duck plague virus (DTMUV), duck Tembusu virus (DPV), and Riemerella anatipestifer, a kit containing this primer set, and their applications. This invention optimizes and establishes a multiplex PCR detection method for DTMUV, DPV, and Riemerella anatipestifer. Specificity results show that the multiplex PCR detection method established in this invention can only amplify specific bands for DTMUV, DPV, and RA, while no amplification bands are found in PCR amplification products using other pathogen DNA or cDNA as templates, resulting in negative results. Specificity test results demonstrate that the multiplex PCR detection method established in this invention has good specificity. Sensitivity test results show that the minimum detection limit for DTMUV cDNA is 9.855 pg, for RA DNA is 0.9 pg, and for DPV DNA is 0.66 pg. This invention provides an effective technical means for the diagnosis of duck Tembusu virus disease, duck plague, and Riemerella anatipestifer disease.

The invention discloses gastric cancer drug sensitivity testing equipment, and relates to the technical field of drug sensitivity testing, the gastric cancer drug sensitivity testing equipment comprises an operation table, the operation table is rotatably provided with a turntable for supporting a culture dish, the guide frame is mounted at the edge of the top surface of the operation table, a supporting table is slidably mounted in the guide frame, and an eccentric table is telescopically arranged on the side surface of the supporting table; a tool sleeve is further obliquely installed at the bottom of the eccentric table, and a coating rod which is matched with the tool sleeve to evenly smear bacterial liquid in the culture dish is inserted into the bottom of the tool sleeve. The sliding base can drive the supporting table to slide down, so that the coating rod below the tool sleeve makes contact with the inner wall of the culture dish, and then the culture dish is driven to rotate along with the rotating disc; and the distance between the eccentric table and the supporting table is adjustable, so that the coating rod displaces, the end of the coating rod can be adjusted to be attached to the side wall of the culture dish as much as possible, and the whole culture dish can be conveniently and completely coated with the bacterial liquid.

Methods for identifying and providing information about inhibiting growth of polymicrobial infections, including but not limited to providing statistics or information about the likelihood of success in inhibiting growth of a polymicrobial infection with particular compositions or therapeutic solutions. The methods herein feature detection and identification of organisms of the polymicrobial sample (e.g., polymicrobial infection), phenotypic pooled sensitivity tests for determining the susceptibility or resistance of the polymicrobial sample (e.g., polymicrobial infection) in the sample to an antibiotic or other therapeutic agent, and identification of resistance genes, e.g., genetic markers that may indicate resistance to a particular treatment. Together, the data can be applied against databases of antibiotic / therapeutic susceptibility or resistance for particular known polymicrobial samples (e.g.. polymicrobial infections) in order to provide information related to the likelihood of success of one or more therapeutic solutions for the polymicrobial sample (e.g.. polymicrobial infection).

The invention belongs to the field of oil and gas field development experiments, and particularly relates to a method for efficiently evaluating reservoir sensitivity characteristics. According to the method, fluid is pumped into a rock core group formed by connecting a plurality of rock cores in parallel for a rock core flow experiment, and the permeability of each rock core in the rock core group is different; the rock core flow experiment comprises the steps of firstly carrying out a speed sensitivity test and then carrying out other types of sensitivity tests; the liquid production flow of each rock core is adjusted to a preset value during the speed-sensitive test, the test is carried out according to the flow velocity from low to high in sequence, and the critical flow velocity is determined; the other types of sensitivity tests are carried out at the temperature lower than the critical flow velocity, and after the flow is stable, the permeability of each rock core is determined according to the produced liquid flow and the pressure difference. According to the method, on the basis of complying with a basic evaluation method of the reservoir sensitivity flow experiment, a whole set of rock core sensitivity evaluation is carried out at the same time, so that the period of the whole reservoir sensitivity evaluation experiment is shortened, and the efficiency is greatly improved.

The present application belongs to the field of biological detection, and particularly relates to a field visual rapid detection method for citrus Huanglongbing. The present application is based on MIRA-lateral flow test paper, takes the outer membrane protein (OMP) of Asian species of Huanglongbing bacteria of citrus as a target, designs and selects specific primers and probes, and prepares dry powder microspheres together with MIRA reaction enzymes, mixes the field rapid extraction plant DNA lysate, dry powder microspheres and Buffer microspheres in a closed chamber to react, adopts a miniature temperature control device to complete amplification under the condition of 42 DEG C for 20 minutes, and directly realizes visual interpretation of the associated test paper strip. The detection method has no cross reaction with other citrus pathogenic bacteria, the sensitivity test can detect the DNA concentration lower limit of 6.9x10 ‑3 ng / μL, the detection device is small in size and convenient to carry, the whole process does not need professional operation or precise instruments, the detection can be completed within 30 minutes, and the detection method is suitable for rapid detection of citrus Huanglongbing by grass-roots agricultural technicians, planting enterprises and fruit farmers.

The invention provides a probe primer composition, a kit and a detection system for rapidly detecting pneumocystis yarrowii. The method comprises the following steps: firstly, respectively designing a plurality of groups of upstream and downstream primers and probes on the basis of characteristic sites (SSU gene and LSU gene in NC020331 (mitochondrial genome) and HSP 70 gene in XM018373242 (genome)), and permutation and combination of the upstream and downstream primers and probes; screening in a conventional PCR (Polymerase Chain Reaction) mode to obtain three sets of primer probe combinations aiming at the three targets; further performing specificity and sensitivity test on the screened primer probe combination, and exploring the lowest detection concentration based on an actual clinical sample, so as to sensitively and specifically detect the pneumocystis yarrowii in the clinical sample on a conventional miniaturized constant-temperature fluorescent PCR instrument.

Provided is a method for evaluating sensitivity of a low-permeability reservoir. The method comprises: using a full-diameter core of a target reservoir to prepare a test sample of the target reservoir; separately performing a standard brine permeability test on each test sample, determining a minimum flow at which each test sample is able to achieve the permeability test to be an initial flow, and then grouping each test sample according to the magnitude of the initial flow; performing a sensitivity test on a same group of samples, comprising: taking a test sample A from a group to be tested, and carrying out a flow rate sensitivity test using the initial flow thereof as a minimum flow, to determine a critical flow rate of the group to be tested; taking test samples B, C, D, E and F from the group to perform water sensitivity, salinity sensitivity, acid sensitivity, alkali sensitivity and stress sensitivity tests, respectively, to obtain water sensitivity, salinity sensitivity, acid sensitivity, alkali sensitivity, and stress sensitivity of the group to be tested, and selecting a critical flow corresponding to the critical flow rate of the group to be tested during each sensitivity test process.

The invention provides an initiating explosive device impact sensitivity test method and system. The method comprises the following steps: carrying out an impact sensitivity test on an initiating explosive device and obtaining test data; when the number of tests reaches a preset activation threshold value and the impact sensitivity test enters a mixed result area, executing a kernel function dynamic selection process; training a Gaussian process regression model by using the selected optimal kernel function to obtain an ignition probability distribution fitting curve of the initiating explosive device in the current test period; calculating an estimated value of a stimulus logarithm corresponding to a group of preset key ignition probability points through a piecewise linear interpolation method, and obtaining a corresponding stimulus estimated value; and calculating the relative change rate of the estimated value of each key ignition probability point. The impact sensitivity test device has the beneficial effects that test parameters are dynamically adjusted through real-time feedback of an impact sensitivity test process and intelligent monitoring of a test result, so that the test frequency is remarkably reduced, the sample consumption is reduced, and the precision and reliability of the test result are improved.

This invention discloses a method for detecting the gas tightness of a ring main unit based on sensor and temperature control calibration, belonging to the field of gas tightness detection technology. It addresses the problem of decreased detection accuracy in low-temperature and high-humidity environments. By deploying pressure and gas concentration sensors and corresponding temperature control sensors within the ring main unit, the surface temperature is monitored in real time, and a heating range is set. The heating device is activated based on the temperature. During heating, detection points are set to collect pressure and gas concentration changes, calculate the rate of change to generate a calibration delay factor, and adjust the calibration time. After heating, an observation time is set to collect temperature drop characteristics and calculate the drop efficiency. Candidate sensors are selected through comparison, and a sensitivity test is performed to calculate the sensitivity temperature coefficient. The drop efficiency is then used to determine whether to enter the calibration state prematurely, thereby improving the measurement accuracy of gas tightness detection in ring main units and the response stability of sensors in low-temperature and high-humidity environments.

The present invention discloses a potato virus X nano-mimetic enzyme test strip and its application. The test strip comprises a backing plate and a sample pad, a conjugation pad, an NC membrane and a blotting paper sequentially attached to the backing plate; the sample pad is pressed against the conjugation pad and is conjugated with a nano-enzyme-labeled monoclonal antibody I; a quality control line and a detection line are provided on the NC membrane; the detection line is conjugated with a monoclonal antibody II paired with the monoclonal antibody I; the specificity is improved by selecting a unidirectional paired antibody; the test results show that the prepared test strip reacts only with PVX-infected plants and does not react with other viruses or healthy plants; the sensitivity test results show that the PVX nano-mimetic enzyme test strip has a sensitivity of 1:10 3 The virus can still be detected after dilution (w / v, g / mL) without false positives, so it can be used for potato virus X detection.

The present invention discloses a PCR primer set for detecting and differentiating avian reovirus and its application, belonging to the technical field of molecular biology. The present invention provides PCR primers for detecting and / or differentiating avian reovirus. The primer set has good specificity for chicken reovirus and no cross-reaction with several other major poultry diseases. The sensitivity test results show that it is about 10 times higher than the conventional PCR method. There is no specific amplification for other common poultry viruses, and no cross-reaction is found. The within-batch and between-batch coefficient of variation are both less than 2%. The detection method of the present invention has the advantages of high sensitivity, strong specificity, and good repeatability.

The invention discloses a multiple PCR (Polymerase Chain Reaction) primer group and a detection kit for simultaneously detecting four pathogens, and also discloses a method for carrying out multiple PCR detection by utilizing the primer group and application of the primer group. Sensitivity test results show that the sensitivity of the mycoplasma bovis, mannheimia haemolytica, pasteurella multocida and mycobacterium bovis detected by PCR has a wide detection range, and the sensitivity can be detected in samples in a certain range. Specific detection results show that only mycoplasma bovis, mannheimia haemolytica, pasteurella multocida and mycobacterium bovis can be well amplified, and other bacteria cannot be amplified, so that the primer group has good specificity.

The present invention relates to the immunotherapy sensitivity test for the prediction of anti-PD-L1 treatment resistance in non-small cell lung cancer.

The invention discloses a ring main unit gas sealing state detection method based on sensors and temperature control calibration, relates to the technical field of gas sealing detection, and is used for solving the problem that the detection precision is reduced in a low-temperature and high-humidity environment. The method comprises the following steps: monitoring surface temperature in real time and setting a heating interval; starting a heating device according to the temperature; setting a detection point to collect pressure and gas concentration changes in a heating process; calculating a change rate to generate a calibration delay factor and adjusting calibration time; and comparing and selecting candidate sensors, executing a sensitivity test to calculate a sensitivity temperature coefficient, and judging whether to enter a calibration state in advance in combination with decline efficiency, so that the measurement precision of ring main unit gas sealing detection and the response stability of the sensors in low-temperature and high-humidity environments are improved.

The invention relates to the technical field of biology, in particular to a primer probe group for detecting avian reovirus and serum type 4 avian adenovirus and application of the primer probe group. According to the invention, an ARV / FAdV-4 double ddPCR detection system and method are developed for the first time on the basis of an S1 gene of an ARV S1133 strain and a hexon gene conserved region of FAdV-4GX2019-010. A sensitivity test shows that the lowest detection limits of ARV and FAdV-4 are 32.67 copies / mu L and 5.27 copies / mu L respectively. Specificity experiments show that the specificity reaches 100%. Clinical sample detection shows that the result is completely consistent with that of qPCR. Therefore, the detection method provided by the invention has the characteristics of high sensitivity, good repeatability, strong specificity and the like, and provides an effective detection technical means for absolute quantitative detection of ARV and FAdV-4.

The present invention discloses a glucocorticoid activation sensitivity test kit, comprising a test kit body, the inner surface of which is equipped with a test tube rack. The present invention provides a glucocorticoid activation sensitivity test kit with a test tube rack that can be flipped back and forth and a side locking device. When in use, the test tube is sleeved on the inner surface of the collar and stored inside the test kit body. During transportation, the handle is pulled outward so that the card slot is clamped on the inner end of the locking rack, and the bracket is in an unlocked state. Therefore, during transportation, the bracket can be driven to shake inside the box body to avoid violent shaking of the test agent, thereby playing a buffering effect. When it is inconvenient to use during testing, the locking device is used to lock the bracket operation, which effectively solves the problem that the fixed bracket structure of the internal reagent of the existing glucocorticoid activation sensitivity test kit during transportation will cause the test reagent to shake inside the reagent tube, resulting in easy overflow of liquid.

The invention discloses LAMP primers for detecting pine wood nematodes, CRISPR RNA, a detection kit and a detection method. The invention relates to a BXEcKs1 gene of bursaphelenchus xylophilus as a target gene, an LAMP primer is designed, corresponding CRISPR RNA is designed, and a detection kit and a detection method for detecting bursaphelenchus xylophilus based on an LAMP-CRISPR / Cas12a system are established. Specificity and sensitivity tests show that the CRISPR RNA as shown in SEQ ID No.8 has the best detection effect, pine wood nematode and bursaphelenchus mucronatus can be specifically distinguished, and the sensitivity reaches 10 fg / L. The detection method for detecting the pine wood nematodes based on the LAMP-CRISPR / Cas12a system has the advantages of being good in stability, high in practicability, high in amplification efficiency and the like, and a sensitive, specific, rapid and visual detection means is provided for rapidly screening the pine wood nematodes on site.

The present application relates to the permeation detection material sensitivity test device, disclose a kind of permeation liquid sensitivity contrast detection device, containing slider (3), sleeve (4), the sleeve (4) is set in the outside of the slider (3), and at least two detection grooves for comparing the permeation liquid sensitivity between the inner surface of the sleeve (4) and the outer surface of the slider (3) are equipped, the slider (3) outer surface is equipped with the through slot (7) for observing the permeation speed of the permeation liquid.The permeation liquid sensitivity contrast detection device is relatively simple to make, repeatedly used multiple times, can guarantee the precision of contrast detection, and can accurately and efficiently draw comparison conclusion by directly observing the permeation condition.

The invention provides a low-permeability reservoir sensitivity evaluation method. The method comprises the following steps: preparing a test sample of a target reservoir by using a full-diameter core of the target reservoir; respectively carrying out standard saline water permeability test on each test sample so as to determine the minimum flow of each test sample capable of realizing permeability test, namely the initial flow, and further grouping each test sample according to the size of the initial flow; carrying out sensitivity test on the same group of samples, namely taking the test sample A in the group to be tested, taking the initial flow of the test sample A as the minimum flow to carry out flow velocity sensitivity test, and determining the critical flow velocity of the group to be tested; respectively taking the B, C, D, E and F test samples from the to-be-tested group to test the water sensitivity, salinity sensitivity, acid sensitivity, alkali sensitivity and stress sensitivity to obtain the water sensitivity, salinity sensitivity, acid sensitivity, alkali sensitivity and stress sensitivity of the to-be-tested group, and selecting the critical flow corresponding to the critical flow velocity of the to-be-tested group as the flow in each sensitivity test process.

The present invention relates to a method for detecting bacteria in a sample and a system for detecting bacteria in a sample, wherein the presence or absence of bacteria can be quickly determined using a well unit provided with a transparent nanoporous thin film without going through a blood culture process after collecting a blood sample of a subject to be tested, and sensitivity tests for various concentrations of various antibiotics can be quickly performed.

The invention discloses a primer and a probe for rapidly detecting mycobacterium paratuberculosis and application of the primer and the probe, and belongs to the technical field of biology. The invention discloses a TaqMan qPCR method, primer and probe for detecting MAP genome nucleic acid aiming at UNI-DUF4185, and an MAP detection method is established by taking a nucleic acid sample of a cattle and sheep vaccine production strain (virus) as a template. Sensitivity test results show that when the recombinant plasmid of UNI-DUF4185 is detected, the lowest detection limit of the detection method is 50 copies / reaction; when the nucleic acid of the MAP genome is detected, the lowest detection limit of the method is 1.12 CFU / reaction. By detecting the recombinant plasmid of UNI-DUF4185, the stability of the established method is evaluated, and the coefficient of variation between batches is within 5%, which is crucial for developing a rapid, sensitive and specific detection method for identifying MAP infected animals and formulating reasonable measures according to the detection result to control the propagation of MAP.

The invention belongs to the technical field of bacterial detection, and discloses a liquid-phase gene chip method for simultaneously detecting Listeria monocytogenes, Staphylococcus aureus, Salmonella and Escherichia coli, which comprises the following specific steps: step 1, designing primers; the method comprises the following steps: downloading an H ly gene sequence of listeria monocytogenes, an FemA gene of staphylococcus aureus, an i nvA gene of salmonella and an rfbE gene of escherichia coli O157: H7; after preparation work of primer design, bacterial DNA extraction and plasmid construction, simultaneous and high-throughput detection of Listeria monocytogenes, Staphylococcus aureus, Salmonella and Escherichia coli can be realized through the detection method established in the step 4, so that the detection time is effectively shortened, the operation is simpler, and the detection efficiency is improved. Meanwhile, a specificity test, a sensitivity test, a repeatability test and a clinical sample detection result show that the detection method disclosed by the invention is higher in sensitivity and lower in specificity and cost, and meets the requirements of rapid on-site real-time detection of the food-borne pathogenic bacteria.

The present application relates to the technical field of sensitivity test of reservoir, in particular to a kind of reservoir salt sensitivity experiment equipment, including hollow cavity for placing core module inside the barrel, the hollow cavity penetrates the bottom of the barrel;Small particles and original fluid discharged in core module are removed by the movement of the scrap circle on the strong magnetic circle driven by the output shaft of telescopic cylinder, to simulate the further diffusion of small particles and original fluid in reservoir pore space and throat outside the sample range under actual working conditions of reservoir, and without using epoxy resin to seal experimental sample, avoid the occurrence of the situation that experimental equipment does not have the ability to simulate actual working conditions of reservoir and cross contamination in traditional salt sensitivity experiment process, can guarantee the authenticity of experimental results, finally small particles, original fluid discharged on the upper end and side end of core module are captured by medium flow path arranged in staggered mode respectively by scrap catching velvet ring, avoid affecting permeability stability measurement.