12 results about "Sensitivity test" patented technology

An interface circuit includes a reference voltage generation circuit to generate a reference voltage, a differential voltage signal generation circuit to convert send data input in sending data into a pair of differential voltage signals and output the pair of differential voltage signals based on the reference voltage generated by the reference voltage generation circuit, a receiver to convert a pair of differential voltage signals input in receiving data and output received data, and a receiver test circuit to perform a sensitivity test of the receiver, the receiver test circuit having a resistance circuit to generate a pair of differential voltage signals having a potential difference being necessary for the sensitivity test of the receiver.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) primer designed by taking a BXAEP1 gene of Bursaphelenchus xylophilus as a target gene, CRISPR (Clustered An LAMP primer and CRISPR RNA are designed aiming at a BXAEP1 gene of the pine wood nematode as a target gene, a detection kit and a detection method for detecting the pine wood nematode based on an LAMP-CRISPR / Cas12a system are established, specificity and sensitivity tests find that the CRISPR RNA shown in SEQ ID No.8 has the best detection effect, the pine wood nematode can be specifically distinguished from pine wood nematode, and the sensitivity reaches 10 fg / L; the established detection method for detecting the pine wood nematodes based on the LAMP-CRISPR / Cas12a system has the advantages of good stability, strong practicability, high amplification efficiency and the like, and a sensitive, specific, rapid and visual detection means is provided for rapidly screening the pine wood nematodes on site.

The invention discloses an LAMP primer designed by taking a BXPC1 gene of bursaphelenchus xylophilus as a target gene, CRISPR RNA and application of the LAMP primer. The invention designs an LAMP (loop-mediated isothermal amplification) primer and CRISPR (clustered regularly interspaced short palindromic repeats) RNA (ribonucleic acid) by aiming at a BXPC1 gene of the pine wood nematodes as a target gene and establishes a detection kit and a detection method for detecting the pine wood nematodes based on an LAMP-CRISPR / Cas12a system. Specificity and sensitivity tests show that the CRISPR RNA as shown in SEQ ID No.8 has the best detection effect, pine wood nematode and bursaphelenchus mucronatus can be specifically distinguished, and the sensitivity reaches 0.1 pg / L; the method for detecting the pine wood nematodes based on the LAMP-CRISPR / Cas12a system has the advantages of being good in stability, high in practicability, high in amplification efficiency and the like, and a sensitive, specific, rapid and visual detection means is provided for rapidly screening the pine wood nematodes on site.

The invention discloses gastric cancer drug sensitivity testing equipment, and relates to the technical field of drug sensitivity testing, the gastric cancer drug sensitivity testing equipment comprises an operation table, the operation table is rotatably provided with a turntable for supporting a culture dish, the guide frame is mounted at the edge of the top surface of the operation table, a supporting table is slidably mounted in the guide frame, and an eccentric table is telescopically arranged on the side surface of the supporting table; a tool sleeve is further obliquely installed at the bottom of the eccentric table, and a coating rod which is matched with the tool sleeve to evenly smear bacterial liquid in the culture dish is inserted into the bottom of the tool sleeve. The sliding base can drive the supporting table to slide down, so that the coating rod below the tool sleeve makes contact with the inner wall of the culture dish, and then the culture dish is driven to rotate along with the rotating disc; and the distance between the eccentric table and the supporting table is adjustable, so that the coating rod displaces, the end of the coating rod can be adjusted to be attached to the side wall of the culture dish as much as possible, and the whole culture dish can be conveniently and completely coated with the bacterial liquid.

Methods for identifying and providing information about inhibiting growth of polymicrobial infections, including but not limited to providing statistics or information about the likelihood of success in inhibiting growth of a polymicrobial infection with particular compositions or therapeutic solutions. The methods herein feature detection and identification of organisms of the polymicrobial sample (e.g., polymicrobial infection), phenotypic pooled sensitivity tests for determining the susceptibility or resistance of the polymicrobial sample (e.g., polymicrobial infection) in the sample to an antibiotic or other therapeutic agent, and identification of resistance genes, e.g., genetic markers that may indicate resistance to a particular treatment. Together, the data can be applied against databases of antibiotic / therapeutic susceptibility or resistance for particular known polymicrobial samples (e.g.. polymicrobial infections) in order to provide information related to the likelihood of success of one or more therapeutic solutions for the polymicrobial sample (e.g.. polymicrobial infection).

The present application belongs to the field of biological detection, and particularly relates to a field visual rapid detection method for citrus Huanglongbing. The present application is based on MIRA-lateral flow test paper, takes the outer membrane protein (OMP) of Asian species of Huanglongbing bacteria of citrus as a target, designs and selects specific primers and probes, and prepares dry powder microspheres together with MIRA reaction enzymes, mixes the field rapid extraction plant DNA lysate, dry powder microspheres and Buffer microspheres in a closed chamber to react, adopts a miniature temperature control device to complete amplification under the condition of 42 DEG C for 20 minutes, and directly realizes visual interpretation of the associated test paper strip. The detection method has no cross reaction with other citrus pathogenic bacteria, the sensitivity test can detect the DNA concentration lower limit of 6.9x10 ‑3 ng / μL, the detection device is small in size and convenient to carry, the whole process does not need professional operation or precise instruments, the detection can be completed within 30 minutes, and the detection method is suitable for rapid detection of citrus Huanglongbing by grass-roots agricultural technicians, planting enterprises and fruit farmers.

The invention provides a probe primer composition, a kit and a detection system for rapidly detecting pneumocystis yarrowii. The method comprises the following steps: firstly, respectively designing a plurality of groups of upstream and downstream primers and probes on the basis of characteristic sites (SSU gene and LSU gene in NC020331 (mitochondrial genome) and HSP 70 gene in XM018373242 (genome)), and permutation and combination of the upstream and downstream primers and probes; screening in a conventional PCR (Polymerase Chain Reaction) mode to obtain three sets of primer probe combinations aiming at the three targets; further performing specificity and sensitivity test on the screened primer probe combination, and exploring the lowest detection concentration based on an actual clinical sample, so as to sensitively and specifically detect the pneumocystis yarrowii in the clinical sample on a conventional miniaturized constant-temperature fluorescent PCR instrument.

The invention provides an initiating explosive device impact sensitivity test method and system. The method comprises the following steps: carrying out an impact sensitivity test on an initiating explosive device and obtaining test data; when the number of tests reaches a preset activation threshold value and the impact sensitivity test enters a mixed result area, executing a kernel function dynamic selection process; training a Gaussian process regression model by using the selected optimal kernel function to obtain an ignition probability distribution fitting curve of the initiating explosive device in the current test period; calculating an estimated value of a stimulus logarithm corresponding to a group of preset key ignition probability points through a piecewise linear interpolation method, and obtaining a corresponding stimulus estimated value; and calculating the relative change rate of the estimated value of each key ignition probability point. The impact sensitivity test device has the beneficial effects that test parameters are dynamically adjusted through real-time feedback of an impact sensitivity test process and intelligent monitoring of a test result, so that the test frequency is remarkably reduced, the sample consumption is reduced, and the precision and reliability of the test result are improved.

This invention discloses a method for detecting the gas tightness of a ring main unit based on sensor and temperature control calibration, belonging to the field of gas tightness detection technology. It addresses the problem of decreased detection accuracy in low-temperature and high-humidity environments. By deploying pressure and gas concentration sensors and corresponding temperature control sensors within the ring main unit, the surface temperature is monitored in real time, and a heating range is set. The heating device is activated based on the temperature. During heating, detection points are set to collect pressure and gas concentration changes, calculate the rate of change to generate a calibration delay factor, and adjust the calibration time. After heating, an observation time is set to collect temperature drop characteristics and calculate the drop efficiency. Candidate sensors are selected through comparison, and a sensitivity test is performed to calculate the sensitivity temperature coefficient. The drop efficiency is then used to determine whether to enter the calibration state prematurely, thereby improving the measurement accuracy of gas tightness detection in ring main units and the response stability of sensors in low-temperature and high-humidity environments.

The invention discloses LAMP primers for detecting pine wood nematodes, CRISPR RNA, a detection kit and a detection method. The invention relates to a BXEcKs1 gene of bursaphelenchus xylophilus as a target gene, an LAMP primer is designed, corresponding CRISPR RNA is designed, and a detection kit and a detection method for detecting bursaphelenchus xylophilus based on an LAMP-CRISPR / Cas12a system are established. Specificity and sensitivity tests show that the CRISPR RNA as shown in SEQ ID No.8 has the best detection effect, pine wood nematode and bursaphelenchus mucronatus can be specifically distinguished, and the sensitivity reaches 10 fg / L. The detection method for detecting the pine wood nematodes based on the LAMP-CRISPR / Cas12a system has the advantages of being good in stability, high in practicability, high in amplification efficiency and the like, and a sensitive, specific, rapid and visual detection means is provided for rapidly screening the pine wood nematodes on site.

The present invention relates to a method for detecting bacteria in a sample and a system for detecting bacteria in a sample, wherein the presence or absence of bacteria can be quickly determined using a well unit provided with a transparent nanoporous thin film without going through a blood culture process after collecting a blood sample of a subject to be tested, and sensitivity tests for various concentrations of various antibiotics can be quickly performed.

The invention discloses a primer and a probe for rapidly detecting mycobacterium paratuberculosis and application of the primer and the probe, and belongs to the technical field of biology. The invention discloses a TaqMan qPCR method, primer and probe for detecting MAP genome nucleic acid aiming at UNI-DUF4185, and an MAP detection method is established by taking a nucleic acid sample of a cattle and sheep vaccine production strain (virus) as a template. Sensitivity test results show that when the recombinant plasmid of UNI-DUF4185 is detected, the lowest detection limit of the detection method is 50 copies / reaction; when the nucleic acid of the MAP genome is detected, the lowest detection limit of the method is 1.12 CFU / reaction. By detecting the recombinant plasmid of UNI-DUF4185, the stability of the established method is evaluated, and the coefficient of variation between batches is within 5%, which is crucial for developing a rapid, sensitive and specific detection method for identifying MAP infected animals and formulating reasonable measures according to the detection result to control the propagation of MAP.