Composite quick colour-developing examination and check agent for coliform group bacteria, researching and developing flow scheme thereof

Abstract

The invention relates to a process for the research and development of a colon-bacillus composite and speedy chromogenic identification reagent. The process is characterized in that: 1. First, the quality control method is determined through activation identification and biochemical identification of coliform groups, salmonella, shigella and staphylococcus aureus; 2. Basic nutrients are selected according to the needs of target strains; 3. It is verified by the auxanogram method that proper nutrition environments, carbon sources, nitrogen sources, minerals, microelements, nutrilits, etc. are all necessary for the growth and breeding of microbes; without any one thereof, the microbes can not grow, metabolize and breed normally; 4. A visualization reagent, a masking reagent and a synergistic agent are selected; 5. Composite selection is conducted; 6. The feasibility of the composite selection formula is verified through tests, verifications and examinations on the indexes of a speedy chromogenic substrate, including the specificity test, the sensitivity test, the real sample inspection, the false negative and false positive strain identification, the stability test, the repeatability test, the competing strain and mixed bacterium interference test, the detection time and detection rate tests. 7. The most suitable formula is selected through a factor level orthogonal combination test.

Description

Coliform complex rapid color diagnosis reagent and its research and development process technical field The invention relates to a food safety rapid diagnosis reagent, in particular to a coliform compound rapid color development detection reagent and a research and development process thereof. Background technique The pure culture method of microorganisms created by the German scientist Koch is still widely accepted by the world microbiology community as an authoritative method for identifying microorganisms because of its low cost, high sensitivity, and the ability to perform qualitative and quantitative specific identification of microorganisms. However, the shortcomings of pure culture are also very obvious. From the preparation of medium, the cultivation of microorganisms, to the formation of growth characteristics that can be distinguished by the naked eye, and the detection of physiological and biochemical indicators of microorganisms to confirm their species characte...

AI Technical Summary

Problems solved by technology

However, it is expensive to purchase rapid detection reagents from such companies, and the application cost is too high. my country cannot afford popular applications. Only some scientific research institutes, universities, and powerful large-scale enterprises can try it, which is difficult for ordinary small and medium-sized enterprises and industrial and commercial people to accept. , and the method of real-time detection combined with instruments is lagging far behind in China, and the purchase of an ordinary rapid detection equipment can cost hundreds of thousands of dollars

Images