African swine fever polymerase chain reaction (PCR) detection method and oligonucleotide primer pair
An African swine fever, oligonucleotide technology
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[0017] 1.1 ASFV P72 plasmid
[0018] Using conventional PCR technology, according to the nucleotide sequence of the African swine fever virus P72 gene published by GeneBank, the full-length ASFV P72 gene was amplified and inserted into the pcDNA3.1 vector.
[0019] 1.2 Source and treatment of tissue disease materials
[0020] Classical swine fever virus (CSFV), porcine reproductive and respiratory syndrome virus (PRRSV), and porcine circovirus type 2 (PCV2) were purchased from Harbin Veken Biotechnology Development Company.
[0021] Clinical samples were collected from sows with reproductive failure or piglets with symptoms of respiratory impairment and aborted fetuses from 14 farms.
[0022] Aseptic treatment of clinical tissue disease materials: cut 2.0g~4.0g of tissue disease materials, add a small amount of sterilized PBS (containing penicillin and streptomycin), cut them into pieces, grind them into paste with a sterile grinder, and press The volume ratio of 1:4 was dil...
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