305 results about "Bird flu" patented technology

The invention provides a preparation method of nanometer cobweb/ nanometer fiber composite protective material, which is characterized by comprising the specific steps of: I, using macromolecule for stirring and dissolving or dispersing spinning into a solvent to obtain an electric spinning material with a weight percentage of 5 to 30 percent; II, inputting the electric spinning material obtained in step I onto a spinneret at a flow speed of 0.1-10mL/h at the room temperature and humidity not more than 30 percent and simultaneously connecting the spinneret with a 10-80 kv power to conduct static spinning; using a layer of base materials to receive the spun threads, covering a layer of top materials on the threads, and then conducting hot-pressing on the threads to obtain the nanometer cobweb/ nanometer fiber multi-layer composite protective material with an aperture of 10-80 nanometer and an average diameter of 15 nanometer. The material can be effectively used for preventing the viruses with diameters between 80 and 120 nanometers of H1N1 influenza A, bird flu, equine influenza, SARS pathogene and the like.

The invention discloses a Chinese medicinal herb additive for egg chickens and application. The Chinese medicinal herb additive comprises membranous milkvetch root, eucommia bark, honeysuckle, jiuyue, Weeping Forsythiae Capsule, szechwon tangshen root, sanguisorba, hawthorn, Indian Buead, officinal magnolia bark, Chinese pulsatilla root, giant knotweed rhizome, ricepaperplant pith, largehead atractylodes rhizome, tangerine peel, malt, indigowoad root, swordlike atractylodes rhizome, indigowoad leaf, three immortals, liquorice, cyrtomium fortune, nutgrass galingale rhizome, chatoyancy, calamus and pinellia tuber, and mixture of the compositions is added with calcium bicarbonate, phytase, vitamin E and methionine. The Chinese medicinal herb additive effectively promotes absorption of nutrient, can prevent and control diseases, strengthen quality of eggshells, reduce the broken egg rate and soft-shell eggs, abnormal eggs and sand shell eggs, can effectively prevent bird flu, prevent and control diseases such as laxness, feather pecking, hair shedding, anus pecking, salpingitis, respiratory disease and the like, can achieve good effect with a dosage of about 4 percent, cannot damage physiological functions of the egg chickens, and can prolong egg laying peak season, improve color of the eggshells and the laying rate by 8 to 20 percent, and increase weight of eggs.

Compounds having unique antiviral and antibacterial properties are prepared from medicinal mushroom mycelium, extracts and derivatives. The compositions are derived from Fomitopsis, Piptoporus, Ganoderma, Inonotus, Trametes, Pleurotus, and blends of medicinal mushroom species and are useful in preventing and treating viruses including Poxyiridae and Orthopox viruses, flu viruses including bird flu (H5N1), SARS and Hepatitis C(HCV), as well as infections from Mycobacterium tuberculosis, Staphylococcus aureus and Escherichia coli.

The invention provides a wool fibre fabric sweat shirt (pants) with functions of powerful antibiosis, cancer prevention and life prolonging by utilizing nano silver, selenium and germanium, belonging to the technical field of healthy wool textile fibre fabrics clothes production. In the real life of people, various germs, viruses and fungi are contacted unavoidably, in particular, various bird flu and HIV discovered currently seriously threaten the health of human beings. Nano silver can kill 650 types of pathogens; far-infrared rays are almost absorbed by the skin of human body completely to lead the surface temperature of the skin to rise; heat energy impels the blood vessel to expand, improves blood circulation and microcirculation; the electromagnetic wave is colorless, tasteless and formless, can easily cause pollution to human body and is a potential killer. The anions in the air are vitamins and longevity elements in the air and are beneficial to human body; selenium and germanium are important elements to prevent and resist cancer; and the mixture of bee series can comprehensively supplement a plurality of nutrient active components required by human body. After nano treatment, the efficacy of anti-aging traditional Chinese medicine can be improved to 100 times.

The invention discloses a Chinese medicinal herb additive for meat chickens and application. The Chinese medicinal herb additive comprises heterophylly falsestarwort root, nutgrass galingale rhizome, amur corktree bark, coptis root, medicated leaven, sanguisorba, hawthorn, Indian Buead, officinal magnolia bark, largehead atractylodes rhizome, tangerine peel, malt, indigowoad root, swordlike atractylodes rhizome, indigowoad leaf, cyrtomium fortune, chatoyancy and calamus; and mixture of the compositions is added with calcium bicarbonate, phytase, mulitivitamin for birds and lysine. The Chinese medicinal herb additive effectively promotes absorption of nutrient, can resist bacteria and virus, strengthen immunity of the meat chickens, effectively prevents bird flu, has obvious curative effect on diseases having respiratory tract symptoms as the major symptom, and has inhibiting function on familiar newcastle disease, colon bacillus, Pasteurella, enteritis, diarrhea, feather pecking, hair shedding, anus pecking, coccidiosis and the like. The additive cannot restrict the growth and disease resistance of the meat chickens, the meat quality of the meat chickens fed by the additive is obviously better than that of the meat chickens fed by the prior feed additive, the average daily gain of the meat chickens is improved by 23 to 28 percent than that of the meat chickens fed by the prior feed additive, the cultivation cycle is obviously shortened, and no harmful substance is deposited.

This invention discloses an nucleotide priemr tested by multiple real-time fluorescence RT-PCR, probe sequence and its method suitable for high-pathogenicity fowl-influenza H5, H7, H9 hypotype, relating to the nucleotide fragment RT-PCR augmenting primer and its complementary sequence, and the parting testing method of fowl-influenza H5, H7, H9 hypotype .

The invention discloses a vaccine strain H5N1/PR8-5B19 marked by H5N1 hypotype poultry influenza virus, which also provides the application to prevent and monitor poultry influenza.

The present invention provides a Chinese medicine for resisting avian influenza virus and its preparation method. Said Chinese medicine is made up by using 9 Chinese medicinal materials of aspidium, isatis root, astragalus root, bupleurum root, forsythia fruit and others through a certain preparation process. Said invention also provides the concrete steps of its preparation process.

The invention discloses a Chinese medicinal herb additive for egg ducks and application thereof. The Chinese medicinal herb additive comprises amur corktree bark, eucommia bark, honeysuckle, szechwon tangshen root, sanguisorba, hawthorn, Indian Buead, officinal magnolia bark, Chinese pulsatilla root, giant knotweed rhizome, ricepaperplant pith, largehead atractylodes rhizome, tangerine peel, malt, indigowoad root, swordlike atractylodes rhizome, indigowoad leaf, three immortals, liquorice, cyrtomium fortune, nutgrass galingale rhizome, chatoyancy and calamus, and mixture of the compositions is added with calcium bicarbonate, phytase, vitamin E and methionine. The Chinese medicinal herb additive effectively promotes absorption of nutrient, can prevent and control diseases, strengthen quality of eggshells, reduce the broken egg rate and soft-shell eggs, abnormal eggs and sand shell eggs, can effectively prevent bird flu, prevent and control diseases such as laxness, feather pecking, hair shedding, anus pecking, salpingitis, respiratory disease and the like, can achieve good effect with a dosage of about 4 percent, cannot damage physiological functions of the egg ducks, and can prolong egg laying peak season, improve color of the eggshells and the laying rate by 9 to 20 percent, and increase weight of eggs.

The invention provides a feed additive prepared from traditional Chinese medicines and used for preventing and treating common diseases of chickens. The feed additive is prepared from the following raw medicines by weight: honeysuckle, rhizoma belamcandae, rhizoma atractylodis, hawthorns, baical skullcap root, rhizoma coptidis, amur corktree bark, radix astragali, radix isatidis, rabdosia rubescens and dandelions. A preparation method of the feed additive is characterized by mixing the above raw medicines and then grinding the mixture or respectively grinding the above raw medicines and then mixing the ground raw medicines. The feed additive has the functions of strengthening body resistance and eliminating evil, clearing away heat and eliminating toxins, diminishing inflammation and strengthening stomach and killing bacteria and pests. By being mixed into the daily feed, the feed additive enhances the immunity of the chicken bodies, improves the disease resistance and has good prevention and treatment effects on common diseases of chickens such as bird flu, newcastle disease, salpingitis, white diarrhea, cholera, ascariasis, laryngotracheitis, coccidiosis, escherichia coli and the like. Through applications, the feed additive has the advantages of good prevention and treatment effects, no toxic or side effect, easily obtained raw materials and low price.

The invention mainly relates to detection of poultry-derived component, which mainly relates to detection of poultry-derived component by using PCR-mtDNA. A specific PCR-mtDNA amplification primers for detecting poultry-derived component is characterized by compring a upstream ribonucleotide with length of 18bp, a downstream ribonucleotide with length of 19bp, and the sequence of the upstream primer PF(18bp) is 5'-AGAACTACGAGCACAAAC-3', and the sequence of the downstream primer PR(19bp) is 5'-GCTATACCTTGACCTGTCT-3'. The detection of poultry-derived component by using PCR-mtDNA has the advantages that: the method is an accurate, rapid and reliable identification method and technology of poultry-derived component, and has the important practical significance of effective resistance on propagation and spread of bird flu and other poultry diseases. The research utilizes aseptic ultrapure water to dilute DNA template of poultry to cause the concentration to decrease to 12ng per Mul, 1.2 ng per Mul, 120 pg per Mul, 12 pg per Mul, 1.2 pg per Mul, 120 fg per Mul, 12 fg per Mul and 1.2 fg per Mul, and carries out PCR amplification. Known from the electrophoresis result of PCR product, the minimum concentration which can be detected is 120 pg per Mul, so the sensitivity of the primer is 120 pg per Mul.

The present invention relates to liquid phase chip method of detecting bird flu virus H5N1 subtype. The liquid phase chip technology includes the homogeny analysis on all the H5 and N1 serum subtype nucleic acid sequence capable of being searched in the nucleic acid sequence bank by means of biological informatic means to find out conservative region, design degenerate primer and specific probe on the H5 and N1 gene fragment and couple the specific probe with fluorescent coding microsphere to make specific detecting microsphere as the liquid phase chip; and recognizing the H5 and N1 gene fragment specifically with the liquid phase chip through two rounds of PCR reaction and reading out the detection result in the chip detection instrument. The method can detect bird flu virus H5N1 subtype quickly for early diagnosis.

The invention aims at providing a new castle disease and H9 subtype bird flu bivalent vaccine. The new castle disease and H9 subtype bird flu bivalent vaccine contains antigens and adjuvant. The antigens are inactivated H9 subtype bird flu viruses and new castle disease viruses. The H9 subtype bird flu viruses are QDY strains, and the preservation number of the H9 subtype bird flu viruses is CCTCC v201517. The QDY strains of the H9 subtype bird flu viruses and a Lasota strain of the new castle disease viruses are inoculated to chick embryos respectively, and then virus liquid is collected; the virus liquid and the oil adjuvant are mixed and emulsified into the vaccine after the virus liquid is inactivated through a formaldehyde solution. The new castle disease and bird flu bivalent inactivated vaccine is good in immunogenicity, antibody production is fast after immunity, the produced antibody titer is high, the produced antibody holding time is long, the retention period is long, the immunizing dose is small, the selected adjuvant is easy to inject, and two kinds of diseases can be prevented through one-time injection. The vaccine has the advantages of being efficient and good in safety.

The present invention discloses a reagent box for quickly detect chicken and birds flu virus antibody. This reagent box includes body, 96 aperture enzyme mark bar in the box, test paper and sample diluted liquid. The test paper consist of sample cushion, absorb cushion, besmear IgG Fc golden cushion, bundled with NP decect string and IgG quality string pyroxylin film, in turn absorb cushion, pyroxylin film, golden cushion, sample cushion plaster unsopped water supported slice. This invention relates to a kind of special monoclonal antibody cross bred tumour cell BDRPDP and special identify IgGFc monoclonal antibody preparation. The reagent box which detect chicken and birds flu virus has strong diathesis, higher sensitivity, simply manipulation and diagnose quickly distinct characteristic.

The invention discloses an H7 subtype bird flu virus RT-LAMP (Reverse Transcriptase Loop-Mediated Isothermal Amplification) test kit and application thereof. The H7 subtype bird flu virus RT-LAMP test kit comprises a Bst DNA, a Bst buffering liquid, AMV (Alfalfa Mosaic Virus) reverse transcriptase, dNTPs, betaine, magnesium sulfate, fluorescent dye, an RT-LAMP primer and distilled water, wherein the RT-LAMP primer consists of a pair of external primers, a pair of internal primers and a pair of ring primers, wherein the pair sequences of the external primers are shown in the SEQ ID No.1 and SEQ ID No.2; the pair sequences of the internal primers are shown in the SEQ ID No.3 and the SEQ ID No.4; and the pair sequences of the ring primers are shown in the SEQ ID No.5 and the SEQ ID No.6. The H7 subtype bird flu virus RT-LAMP test kit has the characteristics of small amount of samples, high specificity, high sensitivity, fastness and accuracy and the like, and provides a simple, convenient and effective early quick testing way for a prevention, control and early warning mechanism of H7 subtype bird flu.

The invention provides a preparation method for a triple inactivated vaccine of recombinant Newcastle disease, bird flu and infectious bronchitis. The technical scheme is realized by gene modification of a Newcastle disease virus (DNV) LaSota strain. A recombinant NDV low virulent strain (rLaSota strain) is taken as a vector, firstly an S1 gene of a chicken infectious bronchitis virus (IBV) is inserted between F-HN, a recombinant NDV rLaSota/S1 expressing an S1 protein is constructed by utilizing a reverse genetic technology, and a result shows that rLaSota can serve as a live vector for stably expressing the S1 protein; secondly a recombinant NDV rLaSota/S1-HA expressing an HA gene is constructed, namely, the HA gene is inserted between P-M of the recombinant NDV rLaSota/S1; and thirdly immunogenicity assessment is performed. A result shows that the triple inactivated vaccine of the recombinant Newcastle disease, the bird flu and the infectious bronchitis, prepared through a conventional inactivated vaccine process has relatively good protection effects for the Newcastle disease, the bird flu and the infectious bronchitis.

The invention discloses a method for detecting a system for quickly screening a fever pathogen, and mainly relates to detection of a tuberculosis antibody, a flu antibody, a bird flu antibody, a plague antibody and an SARS antibody in blood serum. The chip mainly comprises coding microspheres, a coating antigen, a detection antibody, a biotinylated antibody and a streptavidin-phycoerythrin. The method comprises the following steps that: the coating antigen and the coding microshperes are coupled; the detection antibody is respectively coupled with the corresponding microspheres in separate specificity; the red laser excites the classified fluorescence on the spherical substrate; the type is determined according to different colors of the spherical substrates, wherein the biotinylated antibody is combined with the detection antibody; and the streptavidin-phycoerythrin is combined with the biotin of the detection antibody captured by the microshperes; the green laser excites the phycoerythrin, the number of report fluorescence molecules combined on the spherical substrate is measured, and the number is used for indirectly determining the content of the detection antibody combined onthe spherical substrate, so that whether the pathogen infection exists is determined, and the aim of quickly screening the fever pathogen is achieved.

The invention discloses a remote on-line monitoring, sampling and rapid checking system of poultry influenza epidemic situation, which comprises the following parts: water sample self-collector, test paper box, CCD cam, single-chip set and wireless network transmission, wherein the single-chip set of water sample self-collector drives the test paper box to load the immune colloidal gold test paper automatically; the CCD cam transmits the image of immune colloidal gold test paper and water sample reaction to the monitor center computer through wireless network transmission system to judge the reaction image. The invention can do parting identification of sample directly without differentiating, which provides sthenia criterion for rapid decision of epidemic situation.

The present invention provides a kind of Chinese medicine composition for treating avian flu and belongs to the field of avian disease treating Chinese medicine composition. The Chinese medicine composition is prepared with honeysuckle powder, forsythia powder, notopterygium powder, balloonflower root powder, red peony root powder and other Chinese medicinal materials in certain proportion. It has high and fast curative effect on avian flu, no medicine residue and no resistance. It is used in preventing and treating avian flu, and may be also used in treating other avian diseases caused by various kinds of pathogens.

The present invention relates to one kind of recombinant Newcastle disease Lasota low virulent vaccine strain expressing wild or mutant bird flu virus H5 subtype HA protein, and is especially recombinant Newcastle disease Lasota low virulent vaccine strains prLasota-H5wtHA and rLasota-H5mutHA. The present invention also discloses the preparation process of the recombinant Newcastle disease Lasota low virulent vaccine strain and the application of the recombinant Newcastle disease Lasota low virulent vaccine strain in preparing vaccine for preventing bird flu.

The invention relates to a compound immunoenhancement agent and an application thereof on preparing a vaccine for birds. The compound immunoenhancement agent contains 5ng-10mg/mL of poly IC, 10ng-10mg/mL of muramyl dipeptide, 10ng-5mg/mL of levamisole, 10ng-5mg/mL of resiquimod and 10ng-5mg/mL of imiquimod. After the immunoenhancement agent and H5 hypotype bird flu inactivated vaccine are mixed together, antibody can be produced one week earlier, and the antibody titer is improved above 1.8log2. After chicken are immunized through H5 hypotype bird flu inactivated vaccine, H9 hypotype bird flu vaccine or infectious bronchitis vaccine which contains the compound vaccine immunoenhancement agent, the window phases of the antibody production are shortened, the antibody titer of the vaccine is improved, the immunization duration is prolonged, and the probability of infection disease is reduced.