5 results about "Reassortment" patented technology

The invention provides a method and a kit for quantitative detection of recombinant redistribution of a Databa virus based on a multiple digital PCR (Polymerase Chain Reaction) technology, and belongs to the field of infectious disease monitoring. The method comprises the following steps: providing a to-be-detected sample containing segmented viruses and viral nucleic acid; detecting nucleic acid by using a plurality of multiple digital PCR reaction systems containing specific primer pairs and fluorescence labeled probes; amplifying according to a fluorescence coding rule, collecting and analyzing multi-channel fluorescence signals, and calculating the absolute copy number of each genotype; and based on copy number analysis genotype intra-fragment distribution proportion and inter-fragment combination mode, evaluating characteristics and frequency of virus recombination reconfiguration events. According to the detection method and the kit provided by the invention, accurate quantification of all main genotypes of the Databa virus can be realized.

The application belongs to the field of intelligent medical treatment, and particularly relates to a construction method of a segmented virus gene reassortment host adaptability prediction model. The method comprises the following steps: acquiring a virus data set and a host label; filtering the codon importance of different genes in the virus in the virus data set based on a codon importance threshold value of the different genes to obtain key codons of the different genes, combining data representation of the key codons of the different genes to obtain a simulation sequence of the virus data set; and inputting the simulation sequence of the virus data set and the host label into a neural network for iterative training to obtain the virus gene reassortment host adaptability prediction model. The simulation reassortment of the virus is realized based on the key codon region, and the human adaptability of the reassorted sequence is predicted based on the virus reassortment adaptability prediction model. The application can identify the reassortment result that is greater threat to human from a plurality of reassortment possibilities in time, so as to facilitate early targeted research and prevention of the reassortment result that is greater threat to human.

The invention discloses a rotavirus reassortment attenuated strain LG12 and application of the rotavirus reassortment attenuated strain LG12. The rotavirus reassortment attenuated strain LG12 comprises: i) a coding gene of a structural protein VP7 of a human G12 type rotavirus strain G12P [6]; and ii) coding genes of structural proteins VP1, VP2, VP3, VP4 and VP6 and non-structural proteins NSP1, NSP2, NSP3, NSP4 and NSP5 of the sheep rotavirus strain LLR. The reassortment strain provided by the invention has the safety of animal strains and the immunogenicity of human G12 type RV, can effectively prevent infection of human G12 type wild strains, and can have different degrees of cross protection on infection of other various G type RV wild strains; the method has great significance for developing RV vaccines which cover more G and P genotypes of Chinese epidemic RVA, have stronger prevention pertinence and provide a better protection effect on Chinese infantile diarrhea.

The invention belongs to the technical field of pathogen molecular diagnosis and high-throughput sequencing, and particularly relates to a novel bunyavirus whole genome capturing and sequencing method and kit for low virus load and high host background samples. Through the collaborative design of host nucleic acid reduction and two rounds of capture and enrichment, under the condition that the proportion of reads of an initial host source is greater than 90%, a high-coverage sequencing result of high-proportion base sites in L / M / S three fragments of the novel bunyavirus can still be obtained at a relatively low sequencing depth, the coverage integrity and the sequencing sensitivity of a whole genome are remarkably improved, and the method is suitable for large-scale popularization and application. And the sequencing cost and the result volatility are effectively reduced. Reliable whole genome data support can be provided for molecular typing, reassortment / recombination identification, aggregation epidemic situation analysis and transmission chain tracing of the novel bunyavirus, and the method has important clinical and public health application value.

The application provides a quantitative detection method and kit for recombinant reassortment of dabie bunyavirus based on multiple digital PCR technology, and belongs to the field of infectious disease monitoring. The method comprises the following steps: providing a to-be-detected sample containing segmented viruses and viral nucleic acids; detecting the nucleic acids by using a multiple digital PCR reaction system containing multiple specific primer pairs and fluorescently labeled probes; amplifying and collecting and analyzing multi-channel fluorescence signals according to a fluorescence coding rule, and calculating absolute copy numbers of each genotype; and analyzing the distribution proportion within the genotype fragments and the combination mode between the fragments based on the copy numbers, and evaluating the characteristics and frequency of the viral recombinant reassortment event. The detection method and kit provided by the application can realize accurate quantification of all main genotypes of dabie bunyavirus.