9 results about "Reassortment" patented technology

The invention relates to the technical field of bird flu host species identification, in particular to a new bird flu high-risk host species identification method based on virus sharing entropy. According to the method, on the basis of spatial distribution prediction of bird flu host species, the bird flu virus sharing entropy of the bird flu host species is calculated species by species according to the overlapping areas of the target host species and all other host species by extracting the distribution area overlapping area of the target host species and any other host species, and the bird flu virus sharing entropy of the bird flu host species is obtained. And quantitatively analyzing the possibility that the target host species and other host species are subjected to avian influenza cross-species transmission infection. By comparing and analyzing the entropy value of the shared entropy of the avian influenza virus, the avian influenza host species with high virus reassortment risk are identified, and a scientific basis is provided for prevention and control of epidemic diseases.

The invention provides a method and a kit for quantitative detection of recombinant redistribution of a Databa virus based on a multiple digital PCR (Polymerase Chain Reaction) technology, and belongs to the field of infectious disease monitoring. The method comprises the following steps: providing a to-be-detected sample containing segmented viruses and viral nucleic acid; detecting nucleic acid by using a plurality of multiple digital PCR reaction systems containing specific primer pairs and fluorescence labeled probes; amplifying according to a fluorescence coding rule, collecting and analyzing multi-channel fluorescence signals, and calculating the absolute copy number of each genotype; and based on copy number analysis genotype intra-fragment distribution proportion and inter-fragment combination mode, evaluating characteristics and frequency of virus recombination reconfiguration events. According to the detection method and the kit provided by the invention, accurate quantification of all main genotypes of the Databa virus can be realized.

The application belongs to the field of intelligent medical treatment, and particularly relates to a construction method of a segmented virus gene reassortment host adaptability prediction model. The method comprises the following steps: acquiring a virus data set and a host label; filtering the codon importance of different genes in the virus in the virus data set based on a codon importance threshold value of the different genes to obtain key codons of the different genes, combining data representation of the key codons of the different genes to obtain a simulation sequence of the virus data set; and inputting the simulation sequence of the virus data set and the host label into a neural network for iterative training to obtain the virus gene reassortment host adaptability prediction model. The simulation reassortment of the virus is realized based on the key codon region, and the human adaptability of the reassorted sequence is predicted based on the virus reassortment adaptability prediction model. The application can identify the reassortment result that is greater threat to human from a plurality of reassortment possibilities in time, so as to facilitate early targeted research and prevention of the reassortment result that is greater threat to human.

The invention discloses a human-sheep rotavirus gene reassortment attenuated strain LG1 and application of the human-sheep rotavirus gene reassortment attenuated strain LG1. The human-sheep rotavirus gene reassortment attenuated strain LG1 comprises: i) a coding gene of a structural protein VP7 of a human G1 type rotavirus HN15D2 strain; and ii) coding genes of structural proteins VP1, VP2, VP3, VP4 and VP6 and non-structural proteins NSP1, NSP2, NSP3, NSP4 and NSP5 of the sheep rotavirus strain LLR. The human-sheep rotavirus gene reassortment strain which takes a sheep rotavirus strain as a skeleton, contains a human G1-VP7 gene and can be stably passaged on a Vero cell is successfully constructed for the first time, and the reassortment strain not only has the safety of an animal strain, but also has the immunogenicity of a human G1 type RV, can effectively prevent infection of a human G1 type wild strain, and has a good application prospect. The method has important significance for developing multivalent RV vaccines containing G1 genotypes and developing RV vaccines which are higher in prevention pertinence and provide a better protection effect on Chinese infantile diarrhea.

The invention belongs to the field of intelligent medical treatment, and particularly relates to a construction method of a segmented virus gene reassortment host adaptability prediction model. The method comprises the following steps: acquiring a virus data set and a host tag; for different genes of viruses in the virus data set, filtering codon importance based on codon importance threshold values of the different genes to obtain key codons of the different genes, and combining datamation characterization of the key codons of the different genes to obtain a simulation sequence of the virus data set; and inputting the simulation sequence of the virus data set and the host tag into a neural network for iterative training to obtain a virus gene reassortment host adaptability prediction model. Simulated reconfiguration of the virus is realized based on the key codon area, and the human adaptability of the reconfiguration sequence is predicted based on the virus reconfiguration adaptability prediction model. And targeted research and prevention can be conveniently carried out on reassortment results which have great threats to people as early as possible.

The invention discloses a rotavirus reassortment attenuated strain LG12 and application of the rotavirus reassortment attenuated strain LG12. The rotavirus reassortment attenuated strain LG12 comprises: i) a coding gene of a structural protein VP7 of a human G12 type rotavirus strain G12P [6]; and ii) coding genes of structural proteins VP1, VP2, VP3, VP4 and VP6 and non-structural proteins NSP1, NSP2, NSP3, NSP4 and NSP5 of the sheep rotavirus strain LLR. The reassortment strain provided by the invention has the safety of animal strains and the immunogenicity of human G12 type RV, can effectively prevent infection of human G12 type wild strains, and can have different degrees of cross protection on infection of other various G type RV wild strains; the method has great significance for developing RV vaccines which cover more G and P genotypes of Chinese epidemic RVA, have stronger prevention pertinence and provide a better protection effect on Chinese infantile diarrhea.

The invention belongs to the technical field of pathogen molecular diagnosis and high-throughput sequencing, and particularly relates to a novel bunyavirus whole genome capturing and sequencing method and kit for low virus load and high host background samples. Through the collaborative design of host nucleic acid reduction and two rounds of capture and enrichment, under the condition that the proportion of reads of an initial host source is greater than 90%, a high-coverage sequencing result of high-proportion base sites in L / M / S three fragments of the novel bunyavirus can still be obtained at a relatively low sequencing depth, the coverage integrity and the sequencing sensitivity of a whole genome are remarkably improved, and the method is suitable for large-scale popularization and application. And the sequencing cost and the result volatility are effectively reduced. Reliable whole genome data support can be provided for molecular typing, reassortment / recombination identification, aggregation epidemic situation analysis and transmission chain tracing of the novel bunyavirus, and the method has important clinical and public health application value.

The invention discloses application of a novel neuraminidase pseudovirus serum polyclonal antibody in screening H3N2 seasonal influenza vaccine strain products, and belongs to the technical field of biological medicines. The method comprises the following steps: constructing an A H1N1 maternal strain surface antigen NA gene pseudovirus; and then preparing a serum polyclonal antibody with strong specificity and high titer by utilizing the constructed pseudovirus. The novel neuraminidase pseudovirus serum polyclonal antibody is applied to screening of H3N2 seasonal influenza vaccine strain products, and particularly, a high-titer A / PR8 / 34-NA serum antibody is prepared and used for screening of chick embryo high-yield reassortment vaccine strains. Through the technology, the immunogenicity of the NA protein can be effectively improved, the titer of the NA antibody in PR8 totivirus antiserum is improved, and the screening efficiency and accuracy of the chick embryo high-yield reassortment vaccine strain are improved.

The application provides a quantitative detection method and kit for recombinant reassortment of dabie bunyavirus based on multiple digital PCR technology, and belongs to the field of infectious disease monitoring. The method comprises the following steps: providing a to-be-detected sample containing segmented viruses and viral nucleic acids; detecting the nucleic acids by using a multiple digital PCR reaction system containing multiple specific primer pairs and fluorescently labeled probes; amplifying and collecting and analyzing multi-channel fluorescence signals according to a fluorescence coding rule, and calculating absolute copy numbers of each genotype; and analyzing the distribution proportion within the genotype fragments and the combination mode between the fragments based on the copy numbers, and evaluating the characteristics and frequency of the viral recombinant reassortment event. The detection method and kit provided by the application can realize accurate quantification of all main genotypes of dabie bunyavirus.