5 results about "Turkey Herpesvirus" patented technology

The present application relates to an avian cell line capable of supporting viral growth of Marek's Disease Virus (MDV), including Herpes Virus of Turkeys (HVT), methods of producing such cell lines, and therapeutic uses of the cell lines and resulting vaccines.

The invention belongs to the technical field of biology, and discloses a recombinant turkey herpesvirus, and a tandem epitope expression cassette is inserted between UL45 and UL46 of the recombinant turkey herpesvirus; the tandem epitope expression cassette is used for expressing a plurality of B cell epitopes and a plurality of T cell epitopes. According to the recombinant herpesvirus of turkeys, a tandem epitope is inserted into an HVT (herpesvirus of turkeys) vector, and experiments prove that the recombinant herpesvirus of turkeys can induce higher HI antibody and neutralizing antibody titer, obviously stimulate spleen T lymphocyte response of immunized chicken, and can generate an obvious challenge protection effect in 3 days; the challenge protection effect is obviously superior to that of a combined immunization scheme of H9N2 AIV multi-epitope recombinant baculovirus (BV-BNT) and InV provided by the applicant; meanwhile, the recombinant turkey herpesvirus is verified to be capable of performing intraembryonic vaccination and generating an obvious immune protection effect. Meanwhile, the invention further provides a preparation method and application of the recombinant turkey herpesvirus.

The invention belongs to the technical field of biology, and discloses construction and application of a recombinant herpesvirus of turkey for co-expressing an HA gene and an F gene, and the recombinant herpesvirus of turkey for co-expressing the HA gene and the F gene is characterized in that a target gene 1 and a target gene 2 are respectively inserted between an HVT053 site and an HVT054 site and between an HVT065 site and an HVT066 site in a UL region of the herpesvirus of turkey; or respectively inserting the target gene 1 and the target gene 2 between the HVT053 site and the HVT054 site and between the HVT087 site and the HVT088 site in the UL region of the turkey herpesvirus; and respectively obtaining a recombinant virus 1 and a recombinant virus 2. The two recombinant turkey herpesviruses can grow, replicate and stably inherit in vitro, and a good immune protection effect is provided for H9N2 subtype avian influenza and Newcastle disease. Meanwhile, the invention also provides application and a vaccine of the recombinant herpesvirus of turkeys.

The invention belongs to the technical field of biology, and discloses a recombinant turkey herpesvirus, and a tandem epitope expression cassette is inserted between UL45 and UL46 of the recombinant turkey herpesvirus; the tandem epitope expression cassette is used for expressing a plurality of B cell epitopes and a plurality of T cell epitopes. According to the recombinant turkey herpesvirus, tandem epitopes are inserted into an HVT (turkey herpesvirus) carrier, and through experimental verification, good cellular immune response can be induced and generated in 1-day-old chicks and 18-day-old chick embryos by immunizing the multi-epitope recombinant HVT vaccine for one time, and compared with inactivated vaccines needing two-time immunization, the recombinant turkey herpesvirus can provide earlier and better challenge protection effect. Meanwhile, the recombinant turkey herpesvirus is verified to be capable of performing intraembryonic vaccination and generating an obvious immune protection effect. Meanwhile, the invention further provides a preparation method and application of the recombinant turkey herpesvirus.

The present invention relates to recombinant viral vectors for the insertion and expression of foreign genes for use in safe immunization to protect against a variety of pathogens. The present invention also relates to multivalent compositions or vaccines comprising one or more recombinant rival vectors for protection against a variety of pathogens. The present invention also relates to methods for making and using such recombinant viral vectors.