18 results about "Turkey Herpesvirus" patented technology

The present invention relates i.a. to a method for treating or preventing avian influenza virus infections in turkeys comprising administering an effective amount of a rHVT-H5HA COBRA.

The present application relates to an avian cell line capable of supporting viral growth of Marek's Disease Virus (MDV), including Herpes Virus of Turkeys (HVT), methods of producing such cell lines, and therapeutic uses of the cell lines and resulting vaccines.

To provide recombinant viral vectors for the insertion and expression of foreign genes for use in safe immunizations to protect poultry against a variety of pathogens.SOLUTION: Provided are: a recombinant Herpesvirus of turkey (HVT) genome, comprising one or more nucleotide sequences coding for one or more heterologous antigens inserted into an intergenic loci UL35 / UL36 in a unique long region of the HVT genome and one or more nucleotide sequences coding for one or more heterologous antigens inserted at a UL55 / Gene 3 site in the unique long (UL) region of the HVT genome; and a recombinant HVT having the genome.SELECTED DRAWING: Figure 21

The invention belongs to the technical field of biology, and discloses a recombinant turkey herpesvirus, and a tandem epitope expression cassette is inserted between UL45 and UL46 of the recombinant turkey herpesvirus; the tandem epitope expression cassette is used for expressing a plurality of B cell epitopes and a plurality of T cell epitopes. According to the recombinant herpesvirus of turkeys, a tandem epitope is inserted into an HVT (herpesvirus of turkeys) vector, and experiments prove that the recombinant herpesvirus of turkeys can induce higher HI antibody and neutralizing antibody titer, obviously stimulate spleen T lymphocyte response of immunized chicken, and can generate an obvious challenge protection effect in 3 days; the challenge protection effect is obviously superior to that of a combined immunization scheme of H9N2 AIV multi-epitope recombinant baculovirus (BV-BNT) and InV provided by the applicant; meanwhile, the recombinant turkey herpesvirus is verified to be capable of performing intraembryonic vaccination and generating an obvious immune protection effect. Meanwhile, the invention further provides a preparation method and application of the recombinant turkey herpesvirus.

The present invention belongs to the field of biotechnology and discloses two recombinant turkey herpesviruses expressing the fiber protein of fowl adenovirus serotype 4. A target gene is inserted between the HVT053 site and the HVT054 site or between the HVT087 site and the HVT088 site of the turkey herpesvirus, and the target gene is the Fiber-2 gene with a promoter and a terminator to obtain a recombinant virus; the nucleotide sequence of the Fiber-2 gene is as shown in SEQ ID NO.1. The replication ability of the recombinant virus is consistent with that of the parental virus, and it can provide good immune protection against FADV-4 virus, meeting the requirements of a vaccine candidate strain. At the same time, the present invention also provides the target gene, uses and vaccines.

This invention belongs to the field of biotechnology and discloses a recombinant turkey herpesvirus co-expressing the avian influenza H9 gene and the avian adenovirus type 4 Fiber-2 gene. An H9 gene containing a promoter and terminator is inserted between the HVT053 and HVT054 sites of the turkey herpesvirus, and a Fiber-2 gene containing a promoter and terminator is inserted between the HVT087 and HVT088 sites of the turkey herpesvirus, resulting in a recombinant virus. This recombinant turkey herpesvirus stably co-expresses the H9 protein of the H9N2 subtype avian influenza virus and the avian adenovirus type 4 Fiber-2 protein, exhibiting excellent immunogenicity. It effectively improves the immunogenicity of recombinant viruses expressing the Fiber-2 gene of traditional avian adenovirus, without weakening its performance compared to recombinant turkey herpesvirus expressing only either the H9 protein of the H9N2 subtype avian influenza virus or the Fiber-2 protein of avian adenovirus type 4. This invention also provides the uses of this recombinant turkey herpesvirus and a vaccine.

The invention discloses a recombinant herpesvirus turkey for co-expressing chicken infectious anemia virus VP1 and VP2 genes and an infectious bursal disease virus VP2 gene as well as a construction method and application of the recombinant herpesvirus turkey, and belongs to the technical field of medicine or veterinary medicine. According to the invention, by using a recombinant cloning technology, chicken infectious anemia virus VP1 and VP2 gene expression cassettes are inserted between 95322-95323 nucleotides of a turkey herpesvirus FC126 strain, and meanwhile, an infectious bursal disease virus VP2 gene expression cassette is inserted between 112071-112088 nucleotides of a genome of the turkey herpesvirus FC126 strain; and rescuing to obtain the recombinant turkey herpesvirus vaccine strain for co-expressing the chicken infectious anemia virus VP1 and VP2 genes and the infectious bursal disease virus VP2 gene. The invention provides a more convenient and effective technical means for simultaneously preventing chicken infectious anemia and chicken infectious bursal disease, and can achieve the effect of preventing multiple diseases by one injection.

The invention discloses a recombinant turkey herpesvirus strain as well as a construction method and application thereof, and belongs to the technical field of medicine or veterinary medicine. The recombinant turkey herpesvirus is a recombinant turkey herpesvirus strain co-expressing a Newcastle disease virus F gene and infectious laryngotracheitis virus gB, gD and gI genes or a recombinant turkey herpesvirus strain co-expressing a Newcastle disease virus F gene, a chicken infectious bursal disease virus VP2 gene and infectious laryngotracheitis virus gB, gD and gI genes. The recombinant turkey herpesvirus strain obtained by the invention has good in-vitro replication ability and genetic stability. Safety and immunogenicity experiments prove that the two recombinant turkey herpesvirus strains are safe to SPF chickens and have good immune protection effects on NDV virulent strains, ILTV virulent strains and IBDV virulent strains. The invention provides an effective technical means for simultaneously preventing the Newcastle disease and the chicken infectious laryngotracheitis or simultaneously preventing the Newcastle disease, the chicken infectious bursal disease and the chicken infectious laryngotracheitis.

The invention belongs to the technical field of biology, and discloses construction and application of a recombinant herpesvirus of turkey for co-expressing an HA gene and an F gene, and the recombinant herpesvirus of turkey for co-expressing the HA gene and the F gene is characterized in that a target gene 1 and a target gene 2 are respectively inserted between an HVT053 site and an HVT054 site and between an HVT065 site and an HVT066 site in a UL region of the herpesvirus of turkey; or respectively inserting the target gene 1 and the target gene 2 between the HVT053 site and the HVT054 site and between the HVT087 site and the HVT088 site in the UL region of the turkey herpesvirus; and respectively obtaining a recombinant virus 1 and a recombinant virus 2. The two recombinant turkey herpesviruses can grow, replicate and stably inherit in vitro, and a good immune protection effect is provided for H9N2 subtype avian influenza and Newcastle disease. Meanwhile, the invention also provides application and a vaccine of the recombinant herpesvirus of turkeys.

The invention belongs to the technical field of biology, and discloses a recombinant turkey herpesvirus, and a tandem epitope expression cassette is inserted between UL45 and UL46 of the recombinant turkey herpesvirus; the tandem epitope expression cassette is used for expressing a plurality of B cell epitopes and a plurality of T cell epitopes. According to the recombinant turkey herpesvirus, tandem epitopes are inserted into an HVT (turkey herpesvirus) carrier, and through experimental verification, good cellular immune response can be induced and generated in 1-day-old chicks and 18-day-old chick embryos by immunizing the multi-epitope recombinant HVT vaccine for one time, and compared with inactivated vaccines needing two-time immunization, the recombinant turkey herpesvirus can provide earlier and better challenge protection effect. Meanwhile, the recombinant turkey herpesvirus is verified to be capable of performing intraembryonic vaccination and generating an obvious immune protection effect. Meanwhile, the invention further provides a preparation method and application of the recombinant turkey herpesvirus.

The invention discloses a recombinant herpesvirus turkey strain for co-expressing an H9 subtype AIVHA gene, an IBDVVP2 gene and ILTVgB, gD and gI genes as well as a construction method and application of the recombinant herpesvirus turkey strain, and belongs to the technical field of medicine or veterinary medicine. The recombinant herpesvirus of turkeys is obtained by co-transfecting CEF cells with recombinant clay containing H9 subtype AIVHA genes and ILTVgB genes, recombinant clay containing IBDVVP2 genes, recombinant clay containing ILTVgD and gI genes and parent clay containing HVT genome DNA fragments, and then carrying out virus rescue. The recombinant herpesvirus of turkeys is obtained by the following steps: cotransfecting CEF cells with recombinant clay containing H9 subtype AIVHA genes and ILTVgB genes, recombinant clay containing IBDVVP2 genes, recombinant clay containing ILTVgD and gI genes, and parent clay containing HVT genome DNA fragments. The recombinant virus strain obtained by the invention has good in-vitro replication ability and genetic stability. After the SPF chicken is immunized, the SPF chicken can obtain strong immune protection on H9 subtype AIV (avian influenza virus), IBDV (infectious bursal disease virus) and ILTV (Illuminal Low Temperature Virus). The invention provides a new technical means for simultaneously preventing the H9 subtype avian influenza, the infectious bursal disease and the infectious laryngotracheitis.

The invention belongs to the technical field of gene engineering, and particularly relates to application of an MDVgC promoter in construction of a recombinant herpes virus vector vaccine. The heterologous gene expressed by the MDVgC promoter is inserted into the herpes virus, especially turkey herpes virus, Marek's disease virus, porcine pseudorabies virus, bovine herpes virus, duck plague virus, human herpes virus (type 1-8), canine herpes virus or feline herpes virus, and the constructed recombinant herpes virus vector vaccine can efficiently express the heterologous gene and is safe and effective.

The invention discloses a recombinant herpesvirus turkey strain for co-expressing ILTVgB, gD and gI genes, an IBDV classical virulent strain and a novel variant VP2 gene and application of the recombinant herpesvirus turkey strain, and belongs to the technical field of medicine or veterinary medicine. The recombinant turkey herpesvirus is prepared by the following steps: inserting an expression frame CAGW-GB2A2 containing an ILTVgB gene and an IBDV novel variant VP2 gene between UL45 and UL46 genes of a turkey herpesvirus genome, and inserting an expression frame CMV-IBDVC2 containing an IBDV classical virulent strain VP2 gene between UL55 and HVT066 genes of the turkey herpesvirus genome. Meanwhile, an expression framework ILTV-GDGI containing ILTVgD and gI genes is inserted into a US2 gene of a turkey herpesvirus genome to obtain the turkey herpesvirus gene. The recombinant turkey herpesvirus strain can provide a good immune protection effect on ILTV virulent strains, IBDV supervirulent strains and novel variants. The invention provides an effective technical means for preventing the chicken infectious laryngotracheitis and the chicken infectious bursal disease.

The present application relates to an avian cell line capable of supporting viral growth of Marek's Disease Virus (MDV), including Herpes Virus of Turkeys (HVT), methods of producing such cell lines, and therapeutic uses of the cell lines and resulting vaccines.

The present invention relates to recombinant viral vectors for the insertion and expression of foreign genes for use in safe immunization to protect against a variety of pathogens. The present invention also relates to multivalent compositions or vaccines comprising one or more recombinant rival vectors for protection against a variety of pathogens. The present invention also relates to methods for making and using such recombinant viral vectors.

The invention belongs to the technical field of biology, and discloses an influenza HA protein recombinant turkey herpesvirus strain, the preservation number of the recombinant turkey herpesvirus strain is CGMCC No.46642, and the recombinant turkey herpesvirus strain is preserved in China General Microbiological Culture Collection Center; the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing; the preservation date is July 23, 2025. The recombinant turkey herpesvirus has the characteristics that: 1, the recombinant turkey herpesvirus has good safety and immunogenicity, can induce strong body fluid and cellular immune response, can realize early immunization through intraembryonic or 1-day-old inoculation, and reduces interference of maternal antibodies; and 2, the HA gene of the H7 subtype AIV and the virus HA gene of the H5 subtype AIV are inserted into the virus strain, the two genes are independently screened by the applicant, and after being loaded on an HVT vector, the HA gene and the virus HA gene can show relatively comprehensive protection aiming at the corresponding subtype. Meanwhile, the invention also provides application and a vaccine of the recombinant herpesvirus of turkeys.

This invention belongs to the field of biotechnology and discloses a recombinant turkey herpesvirus HVT-H9-IBD expressing two exogenous genes. Target gene 1 and target gene 2 are inserted between the HVT053 and HVT054 sites and between the HVT065 and HVT066 sites in the UL region of the turkey herpesvirus, respectively; or, target gene 1 and target gene 2 are inserted between the HVT053 and HVT054 sites and between the HVT087 and HVT088 sites in the UL region of the turkey herpesvirus. Target gene 1 is an HA gene with a CMV promoter and a bgH terminator, and target gene 2 is a VP2 gene with an mCMV promoter and an SV40 terminator; thus, recombinant virus 1 and recombinant virus 2 are obtained. Both recombinant turkey herpesviruses HVT-H9-IBD expressing the two exogenous genes can grow, replicate, and stably inherit in vitro, providing good immunoprotective effects against H9N2 subtype avian influenza and nVarIBDV. This invention also provides the uses of this recombinant turkey herpesvirus.

The present invention belongs to the field of biotechnology and discloses a recombinant turkey herpes virus expressing the VP2 gene of a new variant of infectious bursal disease virus. The virus is obtained by inserting a target gene into the HVT065 site of the UL region of the turkey herpes virus. The target gene is a VP2 gene with a promoter and a terminator. The VP2 gene is derived from a currently prevalent new variant of infectious bursal disease virus. The present invention discloses the nucleotide sequence of the VP2 gene (SEQ ID NO.1) and the amino acid sequence of a VP2 protein (SEQ ID NO.2). The recombinant virus provided by the present invention has the same in vitro growth and replication ability as the parent virus HVT and can provide good immune protection for the new variant of infectious bursal disease virus. In addition, the present invention discloses a VP2 gene, a target gene, a VP2 protein and its use.