131 results about "Leucosis" patented technology

PCT No. PCT/US96/07370 Sec. 371 Date Nov. 28, 1997 Sec. 102(e) Date Nov. 28, 1997 PCT Filed May 22, 1996 PCT Pub. No. WO96/37625 PCT Pub. Date Nov. 28, 1996Recombinant avian sarcoma leukosis virus (ASLV)-derived retrovirus vectors having an expanded host range are described. The host range is expanded by the replacement of the ASLV envelope gene by an envelope gene from a virus capable of infecting both mammalian and avian cells. The resulting recombinant ASLV-derived retroviral vectors can replicate efficiently in avian cells, infect both avian and mammalian cells in high titer, and are replication-defective in mammalian cells. Thus, they are quite safe and advantageous for use in gene therapy and vaccines.

The invention relates to the production and use of retroviral vectors for cell specific gene transfer, specially to a production method of retroviral vectors containing capsid particles of murine leukemia virus (MLV) and envelope proteins of human immunodeficiency vises (HIV) or simian immunodeficiency viruses (SIV). Said vectors can be used for gene transfer in selected cell types, specially in CD4-positive mammal cells.

The invention provides a GeXP quick detection kit and detection method for simultaneously identifying 8 chicken infectious immunosuppression disease pathogens. The kit is used on the basis of a GeXP system, and comprises 8 pairs of PCR (polymerase chain reaction) primers. The detection result indicates that the kit has the advantages of high specificity and high sensitivity and can be used for simultaneously identifying and detecting chicken Marek's disease virus, A, B and J subgroup avian leucovirus, avian reticuloendothelium hyperplasia virus, avian reovirus, chicken infectious anemia virus and infectious bursal disease virus.

The invention belongs to the cross technical field of molecular immunology and virology, and particularly relates to a hybridoma cell based on hybridoma type leukosis virus subgroup J isolated strain with preservation number of CGMCC (China General Microbiological Culture Collection) No. 5018, a high-specificity monoclonal antibody of avian leukosis virus subgroup J (ALV-J) surface protein (SU) secreted by the hybridoma cell and a preparation method of the monoclonal antibody. By using the high-specificity monoclonal antibody prepared by the invention, a technical support is provided for diagnosis, prevention and treatment of avian leukosis virus subgroup J disease, and the economic loss caused by avian leukosis virus subgroup J disease is effectively reduced.

The invention discloses an anti-avian leukosis virus p27 protein monoclonal antibody, a gold-colloidal strip containing the same, and application. The anti-avian leukosis virus p27 protein monoclonal antibody is generated by secretion of a hybridoma cell strain with a preservation number of CGMCC NO. 11089 or a hybridoma cell strain with a preservation number of CGMCC NO. 11090. The monoclonal antibody is high in titer to p27 protein and good in specificity, and therefore can be used for preparing a kit and the gold-colloidal strip for detecting an avian leukosis virus. A preparation method of the monoclonal antibody provided by the invention is simple, and an antibody purification process is simple, and the efficiency is high, and the cost is low. As the gold-colloidal strip prepared by the anti-avian leukosis virus p27 protein monoclonal antibody provided by the invention is adopted to detect the anti-avian leukosis virus, the specificity is strong, and the operation is simple, and convenience, speediness and simplicity are realized, besides, a special instrument and equipment are not needed, and professional training is not needed, and a result is clear and easy to recognize; the operation is simple and convenient; the popularization is easy, and the antibody is more suitable for real-time detection.

The invention provides an avian leukosis double-antibody sandwich enzyme-linked immuno sorbent assay (ELISA) antigen detection kit. The kit comprises ELISA plates which are coated on anti-avian leukosis virus (ALV) p27 monoclonal antibodies, enzyme labeling anti-ALVp27 monoclonal antibodies and the like. Antibody capturing and antibody detecting are respectively conducted aiming at different antigenic determinants of p27 protein; anti-ALVp27 monoclonal antibodies coated by the ELISA plates are obtained through secretion of hybridoma cell strain ALVP27-5D3, and the enzyme labeling anti-ALVp27 monoclonal antibodies are obtained through secretion of hybridoma cell strain ALVP27-4F12. The avian leukosis double-antibody sandwich ELISA antigen detection kit is easy, convenient and fast to operate, can be used in detecting of all subgroup virus of ALV, and is suitable for all levels of veterinarian departments in the basic level and rapid and mass screening detecting of the avian leukosis of leaving and entering the country. The avian leukosis double-antibody sandwich ELISA antigen detection kit has the advantages of being low in cost, notable in economical benefit, and wide in application prospect.

The invention belongs to the technical field of animal epidemic disease serological diagnosis, and relates to an enzyme-linked immunosorbent assay vector and a kit for detecting avian leukosis P27. The avian leukemia virus (P27) enzyme linked immunosorbent assay kit comprises a 96 hole elisa plate coating a avian leukosis P27 polyclonal antibody, P27 monoclonal antibody marked by alkaline phosphatase, a substrate solution, a stop solution and a cleaning solution. The vian leukosis P27 polyclonal antibody and the P27 monoclonal antibody marked by alkaline phosphatase effectively improve the sensitivity, the specificity and the stability of the detection. The invention provides an efficient and sensitive ELISA (enzyme linked immunosorbent assay) detection kit for sifting out and purifying avian leukosis positive chickens and cultivating new species with heredity resistance, and the kit is low in cost and simple and convenient to operate, and is applicable to promotion in animal husbandry.

The invention discloses a multiple-connection probe amplification detection kit, primer and probe for simultaneously detecting the bluetongue viruses, the infectious bovine rhinotracheitis viruses, the bovine viral diarrhea viruses, the enzootic bovine leucosis viruses and the foot and mouth disease viruses. The multiple-connection probe is shown in sequence tables from SEQ ID NO:1 to SEQ ID NO:10. The primer is shown in sequence tables from SEQ ID NO:11 to SEQ ID NO:12. The primer, the probe and/or the multiple-connection probe amplification detection kit including the primer and the probe can detect the five vital cow disease pathogenies including the bluetongue viruses, the infectious bovine rhinotracheitis viruses, the bovine viral diarrhea viruses, the enzootic bovine leucosis viruses and the foot and mouth disease viruses at the same time, the detection time and cost are saved, and epidemic diseases can be diagnosed in time.

The preparation of tumor immune cell detection chip and detection method relates to a chip for detecting peripheral blood karyocyte of tumor patient and its detection method. The preparation of its chip includes the following steps: on the carrier making chemical modification, namely modifying upper amino-group and aldehyde group; then on the modified microslide on which the chemical gene is set dropping the correspondent antibody, for example, new protein antibody, etc expressed by gene mutation of tumors of carcinoma of lung, live cancer and carcinoma of stomach and skeletal system tumor leucosis, etc. then placing said microslide in humectous environment and storing, then taking out said microslide and washing by using buffer solution. Besides, said invention also provides its detection method by using said chip.

The invention provides a method for labeling antibodies by colorful fluorescent granules and a test paper strip prepared from the antibodies. Animal viruses including, but not limited to, canine distemper viruses, canine parvoviruses, canine adenoviruses, canine coronavirus, rabies viruses, feline leukemia viruses, Marek's disease viruses, Newcastle disease viruses and the like are used as targets, and the corresponding antibodies labeled by colorful fluorescent micro-spheres are prepared by the aid of chemical covalent processes and can be applied to detecting the animal viruses. The method for labeling the antibodies by the colorful fluorescent granules and the test paper strip prepared from the antibodies have the advantages that colorful detection lines with bright developed colors can appear during detection, and accordingly qualitative results can be obtained; the contents of the animal viruses in samples further can be subsequently quantitatively obtained, accordingly, test results are clear and are high in stability, and the test paper strip can be stored at the room temperature for a long time.

The invention discloses a typing method of resistance for resisting a subgroup A avian leukosis virus by quality chickens, which adopts an SNP locus at the 619th site of a TVA gene sequence as a research object; PCR-RLFP analysis is performed to detect whether mutation occurs at the SNP locus, and also to detect whether 4 bases are inserted into SNP loci at the 305th-308th sites of the TVA gene sequence; the two detection results are combined together to determine whether the detected chicken is a genetic resistance type or a genetic susceptibility type. The resistance typing method of the invention performs detection from the source, and omits troubles of later-stage detection; the detection method is rapid, accurate, strong in purposiveness, and simple in operation, has practice significance, and can realize rapid selection of chicken genetic characters and improvement of disease resistance of strains.

The present invention discloses an antitumor multi-drug drug-resistant RNA interference drug, provides a group of nucleotide sequences of interference RNA of therapeutic drug pointed at drug-resistant gene mdr-1 and P-glycoprotein (P-gp) expression and function for resisting leucosis and other tumor. It has the target antitumor cell multidrug drug-resistant (mdr-1) gene and P-glycoprotein (P-gp) expression and function. It raises the sensitivity of leucosis cell conventional chemical therapeutic drug and enhances the action of chemical therapeutic drug for killing malignant tumor cell of hemopoietic system.

The invention relates to a J subset avian leukosis virus rapid detection test paper card which comprises a lining board; a sample pad, a golden-standard pad, a cellulose nitrate membrane and a water-absorbing pad are disposed on the lining board; the invention is characterized in that the golden-standard pad is coated with colloidal gold labelled monoclonal antibodies of mouse anti-J subset avian leukosis virus gp85 protein; the cellulose nitrate membrane is coated with a detection line formed by rabbit anti-J subset avian leukosis virus polyclonal antibodies and a quality control line formed by rabbit anti-mouse IgG polyclonal antibodies. The advantages of the invention are that: the detection method of the J subset avian leukosis virus rapid detection test paper card of the invention can rapidly detect whether a chicken body is infected by or carries J subset avian leukosis viruses, and has the advantages of rapid reaction, high sensitivity, strong specificity, simple operations, suitability for near-the-pen detection, economy and practicality.

The invention discloses a chicken B,D,E-subgroup avian leukaemia genetic resistance related mononucleotide polymorphism molecular marker and application thereof. The molecular marker is TVB gene DNA (deoxyribonucleic acid) sequence SNP-3674C/T, or TVB gene coding sequence SNP-298C/T. The molecular marker can be used for identifying chicken B,D,E-subgroup avian leukaemia genetic resistance characteristics; correspondingly, the molecular marker can be distinguished by a PCR-RFLP (polymerase chain reaction-restriction fragment length polymorphism) process, and can be used for resistance breeding to control the risk of B,D,E-subgroup avian leukaemia from the source; and the molecular marker has the advantages of high purposiveness and the like, is simple to operate and easy to implement, greatly lowers the screening cost, and thus, has important practical meanings.

The invention relates to a method for diagnosing avian leukosis virus subgroup J of sicken chicken flocks and belongs to the technical field of molecular biology. According to the method, C type retrovirus characteristics of the avian leukosis virus subgroup J are taken as a breakthrough point, on the theoretical basis of identifying a JL-2 strain of the avian leukosis virus subgroup J, specific primers are utilized to clone gp85 fragments of the virus strain, an expression vector PGEX-6P-1/gp85 category is constructed, an RFLP method is used to detect the genotype of NRAMP1 genes, the enzyme digestion reaction of the fragments where PCR amplification sites are located is observed, according to the research, it not only is indicated that that cocks carrying T allele have higher resistance to avian leukosis virus subgroup J, but also is found that the specificity diagnosis method established by gp85 is a powerful technical means for detecting specificity of ALV-J and timely purifying populations.

The present invention provides a water soluble tuckahoe mycelium hetero polysaccharide with anti-tumor activity. By using corn slurry as main component to prepare culture medium, tuckahoe, mycelium is cultured through deep fermentation of wild Tuckahoe strain. Water soluble hetereo polysaccharide is extracted from the mycelium with physiological saline and hot water. The hetero polysaccharide consists of alpha-D-glucose, mannitol, galactose and protein in different content. Experiments shows that the hetero polysaccharides can inhibit the growth of Sarcoma 180 tumor in mouse and acute leukovirus cell, so that they may be used as antitumor medicine and health article for raising body's immunological function.

The invention discloses a standard product which is used for detecting the viral load of Friend murine leukemia, and a method which detects the viral load of the Friend murine leukemia by a real-time fluorescent quantitative polymerase chain reaction method (Real-Time RT-PCR method) by using the standard product. The detection process includes the steps of the extraction and content measurement of leukovirus nucleic acid (RNA), the obtaining of the nucleic acid segment by amplifying reverse transcription by using a primer, the detection of the real-time fluorescent quantitative polymerase chain reaction (Real-time PCR), and the like. Compared with conventional PCR detection methods, the method of the invention, which detects the viral load of the Friend murine leukemia, has better specificity. The detected genetic amplified products are target genetic products to be detected. The method has a better linear relationship and is suitable for being applied to the detection of the viral load of the Friend murine leukemia (Fr. MuLV).

The invention discloses a gene chip, a kit and a method for detecting three viruses for immunosuppression disease of chickens, and relates to the field of animal medical diagnosis. The gene chip is characterized in that specific probe sequences of four subgroups A, C, D and J of an ALV (Avian leucosis virus) as well as a CAV (Chicken Anemia Virus), and an REV (Reticuloendotheliosis virus) are arranged on a substrate of the chip. The invention further provides the kit for detecting the four subgroups A, C, D and J of the ALV, the CAV and the REV. The chip, kit and detecting method, provided by the invention can be used for detecting various pathogens quickly and accurately in a high-flux manner, so that infectious diseases can be diagnosed correctly within a relatively short period to be prevented from spreading.

The invention relates to a composition containing selfheal and blackberry lily, which comprises the following raw materials, or extracts of the following raw materials or chemically acceptable derivatives of the extracts of the following raw materials in respective dosage: 10 parts of selfheal and 2 to 60 parts of blackberry lily. On the basis, the following one kind or various kinds of but not limited to the following raw materials can be added according to specific conditions: 3 to 50 parts of rheum officinale, 3 to 50 parts of scutellaria baicalensis, 3 to 50 parts of lithospermum, 3 to 50 parts of radices trichosanthis, 3 to 50 parts of radix bupleuri, 3 to 50 parts of osmundaceae cyrtomium fortunei, 3 to 50 parts of honeysuckle and 3 to 50 parts of cassia twig. The composition can be prepared into medicinally or pharmaceutically acceptable physical forms such as powder, liquid, paste, particles, capsules and the like. The composition is used for preventing and treating viral diseases such as bird flu, newcastle diseases, infective bronchitis, infective leukemia, infective bursal diseases, swine fever, parvovirus diseases, epidemic diarrhea, porcine reproductive and respiratory syndrome and the like, and the mortality rate is reduced.

The invention belongs to the technical field of biomedicine and particularly relates to application of seaweed oligosaccharide in the preparation of an avian leucosis virus resistant preparation. seaweed oligosaccharide is prepared by degrading natural polysaccharides extracted from the macroalga Grateloupia filicina, Ulva lactuca, Sargassum fusiforme and other algae, has a wide source range, is simple to prepare, and has the advantages, as an antiviral preparation, such as naturalness, zero toxicity and environmental friendliness; in-vitro cell experiments prove that the seaweed oligosaccharide is capable of inhibiting the proliferation of avian leucosis virus, has significant antiviral effect, may be applied to livestock and poultry breeding as a novel antiviral preparation, and has high applicable value.

The invention discloses a multiplex detection reagent for immunosuppressive poultry pathogens of avian influenza and the like and an application of the multiplex detection reagent. The multiplex detection reagent comprises six specific probes with sequences shown in Seq ID NO.1-Seq ID NO.6 which refer to AVI virus, ND (newcastle disease) virus, serotype I Marek's disease virus, IBDV (infectious bursal disease virus), avian infectious anaemia virus and ALV-J (aivan leukosis virus subgroup J) in sequence. The reagent comprises the specific probes for six immunosuppressive poultry pathogens comprising AVI and the like and can specifically capture target sequences of the six immunosuppressive poultry pathogens, and the foundation is laid for high-throughput rapid detection of the six immunosuppressive poultry pathogens. With adoption of the reagent, a rapid and accurate detection method is provided for the six immunosuppressive poultry pathogens on the basis of a liquid chip.