30 results about "Cloaca" patented technology

An immobilized microbial consortium is formulated which comprises of a synergistic mixture of all the bacterial strains of Aeromonas hydrophila, Pseudomonas fluorescens, Pseudomonas aeruginosa, Bacillus circulans, Yersinia enterocolitica, Enterobacter cloaca and Bacillus brevis. The formulated microbial consortium is immobilized on a non-biodegradable and economically cheaper support. The said immobilized microbial consortium is used for the biodegradation of synthetic phenol as well as phenol present in petroleum refinery effluent. The results of biodegradation obtained with the microbial consortium immobilized on coconut fiber are compared with those obtained with microbial consortium immobilized on well known support. The coconut fiber used for immobilization proved to be a better support than a well known support such as calcium alginate.

The invention relates to a campylobacter jejuni chitosan nanometer DNA vaccine, a preparation method and application thereof. The campylobacter jejuni chitosan nanometer DNA vaccine is a nano-microcapsule composed of a chitosan and a recombinant plasmid; the recombinant plasmid is a pCAGGS-flaA which consists of a campylobacter jejuni flagellin gene flaA and a eukaryotic expression vector; the flagellin gene flaA has a nucleotide sequence showed by an SEQ NO.1. The campylobacter jejuni chitosan nanometer DNA vaccine is made by dissolving the constructed recombinant plasmid in chitosan solution; the vaccine can also be used for preparing the drugs for preventing chicken campylobacter jejuni. Tests prove that the vaccine can stimulate to produce specific humoral and local mucosal immune response; after oral challenge, the cloaca bacteria exhausting rate shows a significant declining trend, and the number of the campylobacter jejuni in blood, small intestine, large intestine and caecum can be decreased.

The invention relates to a quick detection and diagnostic method for animal derived chlamydophila (Chlamydophilapsittaci, C.abortus, C.caviae, C.pecorum, C.felis) by applying real-time PCR (Polymerase Chain Reaction), which is one of new molecular biological technologies. The detection system designs a pair of primers and corresponding TaqMan probes based on a conserved zone of 16S rRNA of the animal derived chlamydophila. The system can be used for detecting animal derived chlamydophila genes existing in animal excrement, cotton swab of cloaca and human tracheal eluate with the detection sensitivity higher than that of an ordinary PCR by 10 times. The real-time PCR does not need post-PCR treatment, avoids cross contamination caused by the operation of agarose gel electrophoresis and accordingly enhances detection accuracy and reliability.

A method of artificially inseminating a hen includes the steps of securing a male bird, applying a semen extender liquid to a tip of a cloacal protuberance of the male bird, squeezing the cloacal protuberance to extract semen from the male bird into the semen extender liquid to form a mixture, applying a tip of a pipette to the mixture, drawing the mixture into the pipette, securing the hen, inserting the pipette into the hen's cloaca, and squeezing a bulb of the pipette to inject the mixture from the pipette into the cloaca. A holding device for restraining a bird during sperm collection and artificial insemination procedures has a base, an arm extending upward from the base, and an open-ended receptacle connected to the upper end of the arm. The holding device is useful in mitigating injury to the bird and the handler during the procedures.

The present invention is directed to a method for the incubation and hatching of premature eggs, in particular, surgically-derived premature eggs removed from a bird prior to transfer of the premature egg to the cloaca of a bird.

Immobilized cell beads incorporating formulated microbial consortium comprising a synergistic mixture of the following bacterial strains namely, Enterobacter sakazaki, Pseudomonas aeruginosa and Aeromonas sobria selected from the following isolated bacterial strains namely, Yersinia enterocolitica, Aeromonas sobria, Klebsiella pneumoniae, Serratia liquefaciens, Enterobacter sakazaki, Citrobacter amalonaticus, Pseudomonas fluorescens, Pseudomonas aeruginosa, Enterobacter cloaca, Acinetobacter calcoaceticus are prepared, the formulated microbial consortium is immobilized in an appropriate immobilizing agent resulting in the formation of beads and the beads are used for instant BOD estimation using an electronic device and the formulated cell beads are reusable and are capable of assimilating most of the organic matter present in varied industrial effluents.

The invention relates to a reagent box and a detecting method for quickly detecting newcastle disease virus by using loop-mediated isothermal amplification (LAMP) technique, which comprises 10 x isothermal reaction buffer solution, AMV 8U/Mul, Rnasin 40U/Mul, Bst DN polymerase 8U/Mul, 10mM dNTP, 100mM magnesium sulphate, 20 Mu M inner primer 1, 20Mu M inner primer 2, 10Mu M outer primer 1, 10Mu M outer primer 2, the reaction solution A of 5M betaine, and the reaction solution B of the betaine: 1000 x fluorescent dye SYBR Green I. By extracting the RNA of tissue sample as well as the RNA in the poultry throat and cloaca cotton swab sample, conducting the loop-mediated isothermal amplification of newcastle disease virus, and detecting the coloration of the amplified products, the newcastle disease virus can be detected. By eliminating the defects in prior art, such as time-consuming quality, large workload, cross contamination and complex operations, the reagent box and the detecting method for quickly detecting newcastle disease virus has the advantages of strong specificity, high sensitiveness, good speed, low cost, simpler operating methods, and perfect applicability to quick detection at site.

The invention provides a feed additive capable of improving laying fowl egg quality. Each part of the feed additive is characterized by being prepared from 2 to 5 hundred billion of bacillus coagulans, 10 to 20g of mannan oligosaccharide, 200 to 400g of lactobacillus casei metabolite, 200 to 400g of puffed bran and 200 to 300g of carrier. The bacillus coagulans, the mannan oligosaccharide, the lactobacillus casei metabolite, the puffed bran and the carrier are used together, and probiotics are added into the feed, so that the whole digestive tracts of laying fowls are protected from oral cavities, the microecological balance of the intestinal tracts of the laying fowls is built rapidly, the digestion utilization rate of the feed is increased, the occurrence of watery stool and diarrhea is reduced, the content of pernicious bacteria in cloaca is reduced, the content of the lactobacillus casei is increased, and the probability that intestinal tract pernicious bacteria spread to fallopian tubes is lowered. Thus, the feed additive plays a role in stabilizing the laying rate, improving egg quality, and reducing shell-broken eggs and soft eggs.

The invention belongs to the technical field of egg laying recording devices, and provides an egg laying recording device suitable for a cloaca of a female Chinese softshell turtle. The egg laying recording device comprises an upper clamping body and a lower clamping body which are connected into a whole; the upper clamping body is provided with a limiting hole; a nipple-shaped protrusion in the cloaca penetrates through the limiting hole; two sides of the front end of the lower clamping body are inserted into a cavity in the lower end of the protrusion to be matched with the cloaca; connecting buckles are arranged on the upper clamping body on two sides of the lower end of the limiting hole; connecting clamping grooves corresponding to the connecting buckles are formed in the lower clamping body; the connecting buckles are connected with the connecting clamping grooves in a clamped mode; and an egg laying recording device body is arranged on the upper clamping body. Compared with the prior art, the egg laying recording device has the advantages that the egg laying recording device is fed into the cloaca of the Chinese softshell turtle, egg yield of the Chinese softshell turtle is recorded, after a recording period of one year is finished, the upper clamping body and the lower clamping body of the egg laying recording device are separated, the egg laying recording device is taken out, the egg yield of the Chinese softshell turtle can be known through the egg laying recording device, and a selection basis is provided for breeding of Chinese softshell turtles.

The invention provides a method of rearing a bird of germ-free status. The method comprises housing a bird as a parent bird, surgically removing an egg in its shell from the parent bird prior to transfer of the egg to the cloaca in the parent bird, incubating the egg still in its shell and hatching the egg to produce a laying bird. The invention also relates to the production of avian eggs of germ-free status.

The invention provides an improved method of rearing a bird of specified germ- free status. Specifically, the present invention is directed to a method for increasing the hatchability and viability of surgicaliy derived eggs of germ-free status when obtained from a parent bird. The method comprises housing a bird as a parent bird, determining the position of a premature egg in the reproductive tract and stage of development of the premature egg in terms of shell formation and calcification, surgically removing an egg in its shell from the parent bird prior to transfer of the egg to the cloaca in the parent bird, incubating the egg and hatching the egg to produce a laying bird. The invention also relates to the production of avian eggs of specified germ-free status and uses thereof.

The invention belongs to the technical field of dosing devices, and provides a dosing device suitable for the cloaca of a female Chinese softshell turtle. The dosing device comprises an upper clamping body and a lower clamping body which are connected into a whole, wherein the upper clamping body is provided with a limiting hole; a unique papillary protrusion is arranged in the cloaca of the Chinese softshell turtle and can penetrate through the limiting hole; the upper clamping body downwards extrudes the lower clamping body at the papillary protrusion; the front end of the lower clamping body is in a U shape; two sides of the front end of the lower clamping body are inserted into a cavity in the lower end of the protrusion to be matched with the cloaca; connecting buckles are arranged on the upper clamping body and located on two sides of the lower end of the limiting hole; the lower clamping body is provided with connecting clamping grooves corresponding to the connecting buckles; the connecting buckles are connected with the connecting clamping grooves in a clamped mode; and the upper clamping body is further provided with a dosing structure. Compared with the prior art, the dosing device of the invention has the advantages that the dosing device is designed according to the characteristics of the cloaca of the female Chinese softshell turtle, stress and damage to the Chinese softshell turtle caused by frequent medicine feeding are omitted, and the device is simple in structure, convenient to use and applicable to the fields of the artificial insemination technology and the like.

The invention belongs to the technical field of duck feed and in particular discloses laying duck feed for increasing egg output and improving egg quality. The feed is prepared from the following rawmaterials: pearl barley, gorgon euryale seeds, fresh asparagus, corn, salt, broccoli salt, peanut meal, shrimp shells, locusts, vegetable pulp, an allium macrostemon extract, a seville orange flower extract, a bunge cherry seed extract, aesculetin and chlorogenic acid. The feed is balance and rich in nutrition and free of harmful substance residue, nutrient components essential for ducks in layingperiods can be provided, the requirements of enormous appetite of laying ducks can be met, fat accumulation can be also effectively prevented, the phenomenon that the ducks are too fat is avoided, the egg output is effectively increased, the egg quality is effectively improved, fallopian tubes and cloaca mucous epithelium are effectively prevented from cornification, the elasticity of the fallopian tubes and the cloaca mucous epithelium is effectively improved, egg laying smoothness is promoted, and the rectocele phenomenon is prevented.

The invention discloses a trionychidae animal semen delivery device. The trionychidae animal semen delivery device comprises a device body, an expanding unit, a first semen delivery pipe, a second semen delivery pipe and a plunger, wherein inside the device body is provided with a semen delivery channel, the rear end of the expanding unit is provided with a distribution chamber with a three-way function, the distribution chamber comprises a first orifice, a second orifice and a third orifice, the first orifice is connected with the first semen delivery pipe, the second orifice is connected with the second semen delivery pipe, the side wall of the third orifice is in contact with the inner wall of the semen delivery pipe, and the distribution chamber is movably connected with the device body. The trionychidae animal semen delivery device has the advantages that the expanding unit is used for expanding the cloaca of a female pelodiscus sinensis; along with further inward pushing of the plunger, the expanding unit can be expanded off the device body and then retained inside the cloaca of the female pelodiscus sinensis to serve as a plug for preventing sperm inside the cloaca from overflowing; every female individual correspond to one expanding unit to avoid cross infection among the female individuals and improve the fertility rate.

The invention discloses an artificial insemination method for female cherry valley ducks, which is characterized in that the cloaca of a female duck contracts tightly and is difficult to turn out, the lower edge of the cloaca is slightly extruded by three fingers of the left hand and the right hand, and the cloaca opening is slightly pushed aside by the index finger, so that the cloaca is opened, the tension of the female duck is reduced, and the cloaca of the female duck is convenient to open; when a male duck is inseminated, the forefinger of the right hand is slightly and slowly inserted into the cloaca from the cloaca opening, then the vaginal opening is found from the left lower side, the vaginal opening of the female duck can be better found, the number of effective sperms fed is 50 million to 90 million, sufficient insemination amount is fed, the fertilization rate of seminal fluid insemination of the male duck is high, the insemination amount can be increased by one time during first insemination, or insemination is repeated once the next day, the good fertilization rate can be maintained by conducting insemination once every 3-4 days, the fertilization rate can be greatly improved by controlling the insemination time, insemination eggs can be conveniently laid, and the problems that the artificial insemination technology for breeding the cherry valley breeding ducks is poor, and the probability of increasing the yield of the insemination eggs is low are solved.