34 results about "GroEL" patented technology

An ELISA kit for detecting a Salmonella pullorum antibody is established by screening Salmonella pullorum dominant antigen GroEL through an immunoprecipitation technology, expressing GroEL recombinant protein through utilizing a prokaryotic expression vector, and utilizing an antigen protein. The kit can reduce the response of chicken in the detection process of the Salmonella pullorum antibody, and can improve the detection specificity and the sensitivity.

The invention belongs to the field of genetic breeding, and particularly relates to a mutant strain of arthrobacter simplex with stress tolerence and a genetically engineered bacterium constructed bythe mutant strain. The survival rate of the mutant strain under the impact conditions of 16% ethyl alcohol and 20% methyl alcohol is increased by 3.74 times and 2.10 times respectively compared with the original strain; the endurance capacity of engineering bacteria, overexpressing the genes of groEl, dnaK, recA, uvrD, katG, sod or treS from the mutant strain, to high-concentration organic solvents is obviously improved, and the endurance capacity to salt pressure and oxidative pressure is expresses to be higher. When the engineering bacteria are applied to steride C1,2 dehydrogenation reaction, the ethanol concentration in a system can be increased to 8 to 10%, the substrate CA concentration can be increased to 6 to 8g/L, the product yield can be improved by 0.41 to 3.56 times, and a goodbasis is established for the subsequent tolerance mechanism research and bacterial strain molecular modification and application.

The invention discloses an application of Brucella GroEL protein and a method for recombinant expression of Brucella GroEL protein, in particular an application of Brucella GroEL protein used as a protective antigen in preparation of Brucella subunit vaccines, and a process for recombinant expression of the Brucella protective antigen GroEL. The experiment proves that the Brucella GroEL protein can be combined with adjuvants for immunizing to induce generation of specific antibodies and excite cell immunity, and the Brucella GroEL protein has a better immune protection effect on Brucella and can be used for preparing Brucella subunit vaccines. The Brucella GroEL protein disclosed by the invention plays an important role in epidemic prevention of Brucella, and has wide application prospects.

The invention discloses a fusion protein, the amino acid sequence of which contains an iRGD peptide sequence shown as SEQ ID No:1 and a molecular chaperone GroEL sequence shown as SEQ ID No:2. The invention further discloses an encoding gene of the fusion protein, the sequence of which is a nucleotide sequence capable of encoding the fusion protein. The invention further discloses a preparation method of the fusion protein. The preparation method comprises the following step of expressing the encoding gene in a bacterial strain to obtain the fusion protein. A method of preparing a pharmaceutical composition comprises the following steps of contacting the pharmaceutical compound with the fusion protein disclosed by the invention to obtain a contacted material in the presence of a solvent. The invention further discloses the pharmaceutical composition prepared by the method of preparing the pharmaceutical composition. The fusion protein provided by the invention can stably load a hydrophobic drug, and is high in drug-carrying efficiency and good in drug release effect. The pharmaceutical composition prepared by the fusion protein provided by the invention has the advantages of good biocompatibility and good drug release effect.

The invention relates to the technical field of biology and in particular relates to a kit for simultaneously detecting an avian leukosis virus antibody and a salmonella pullorum antibody. An antigencombination of the kit comprises p27 protein, gp85 protein and GroEL-delta8-1 protein. Avian leukosis virus capsid protein p27, prokaryotic expression protein of envelope protein gp85, and prokaryoticexpression protein of truncated GroEL-delta8-1 of a salmonella pullorum dominant antigen are used as a coating antigen to develop an ELISA (Enzyme-linked Immunosorbent Assay) kit capable of simultaneously detecting the avian leukosis virus antibody and the salmonella pullorum antibody. Compared with a current common kit for independently detecting avian leukosis or pullorum disease, the kit provided by the invention has the effect of simultaneously detecting the avian leukosis or the pullorum disease, and the detection and purification work of the avian leukosis and the pullorum disease can be extremely alleviated.

The invention relates to the technical field of animal epidemic disease detection and in particular relates to a kit for simultaneously detecting an avian leukosis virus antibody and a salmonella pullorum antibody. The kit comprises: P27 protein, GroEL-delta8-1 protein, an enzyme labeled second antibody, BSA (Bovine Serum Albumin), coating liquid, confining liquid, a color developing solution anda stopping solution. The invention develops an indirect ELISA (Enzyme Linked Immunosorbent Assay) kit capable of simultaneously detecting the avian leukosis virus antibody and the salmonella pullorumantibody by taking prokaryotic expression protein of a conserved region P27 of an avian leukosis virus and prokaryotic expression protein of truncated protamine GroEL-delta8-1 of a dominant antigen GroEL of salmonella pullorum as coating antigens; the kit provided by the invention can be used for simultaneously detecting avian leucosis and pullorosis; chickens which are detected to be positive areeliminated so that the aim of simultaneously purifying is realized and the clinical detection and purification work is extremely alleviated.

The invention discloses a dominant T-and B-cell T-B combined multivalent antigen epitope fusion gene and a prokaryotic expression system thereof by constructing leptospira interrogans LipL21, LipL32,groEL and loa22 proteins. The MAP vaccine is prepared by the following steps of: expressing a multiple antigen peptide (MAP) consisting of antigen epitopes, and artificially crosslinking the MAP witha high polymer core carrier. The prepared MAP vaccine is has good immune effect, is low-cost, and is capable of effectively inducing cellular immunity. Toxic and side effects of the vaccine are reduced, safety is improved, and immune pertinence is enhanced.

The invention provides a preparation method of protein nanocage stabilized Pickering emulsion in order to overcome defects that nano-particle stabilized Pickering emulsion is poor in biocompatibilityand biodegradability and common bioorganic molecules are poor in surface activity, have insufficient monodispersity and wetting performance and are easily affected by environmental conditions, and belongs to the field of Pickering emulsion. According to a specific scheme, a buffer solution of a GroEL protein nanocage is prepared, and mass fraction of GroEL is controlled to be 0.05wt%-0.45wt%; an oil-phase substance and water are mixed in a certain proportion, and the range of a volume ratio of the oil-phase substance to water is controlled to be 0.11-0.50; the GroEL solution is added to an oil-water mixture dropwise, meanwhile, the mixture is stirred, ultrasonic treatment is performed, and crude emulsion is enabled to be homogenized. The invention further provides an application of the Pickering emulsion as a bioactive substance for carrier encapsulating or transport. The Pickering emulsion prepared on the basis of the unique structure and excellent interfacial activity of GroEL has the advantages of good stability, long quality guarantee period, high biocompatibility and simple preparation process and is environmentally friendly.

It is an object of the present invention to a method whereby a humoral immune response is induced more efficiently in producing an antibody against an antigen protein by gene immunization. A fusion gene composed of a gene encoding the full-length of a part of the antigen protein or a gene encoding a chaperonin subunit or a chaperonin subunit linkage linked thereto is administered to express the fusion gene in the animal, thereby inducing a humoral immune response to an antigen protein by administering. An example of the chaperonin includes Escherichia coli GroEL. There is also provided with a composition for immunization, a method for producing an antibody, a method for producing a hybridoma, and a method for producing a monoclonal antibody.

The invention discloses a phage Phi X174 lytic cell protein E and application thereof and belongs to the field of genetic engineering. The method in the invention comprises the following steps: takingrecombinant Escherichia coli E. coli (PAI/GroELS) as an original strain, introducing a phage Phi X174 lytic cell protein E, producing holes in the surfaces of recombinant Escherichia coli cells, andreleasing linoleate isomerase from the cells by utilizing osmotic pressure differences inside and outside the cells. Finally, by optimizing the release conditions, 81% of linoleate isomerase in the cells is released out of the recombinant Escherichia coli cells.

The invention relates to the technological field of biotechnology, and especially relates to an application of a heat shock protein groEL to preparation of a reagent for detecting Helicobacter pylori.The application of the heat shock protein groEL to a marker for detecting Helicobacter pylori is provided; experiments confirm that heat shock protein groEL is used as the marker for detecting Helicobacter pylori, detection rate of Helicobacter pylori virulence type is greatly improved, the accuracy rate reaches 98% or above, the maker is not interfered by non-virulence type helicobacter pylori or other pathogens, and the maker has good specificity.

This invention relates generally to compositions and methods for detection of pathogenic bacteria that express extracellular chaperonin proteins. In one embodiment, it relates to Mycobacterium tuberculosis (Mtb) detection and provides for novel probes for a specific and sensitive diagnostic test for Mycobacterium tuberculosis complex (TBC) that hybridize with the groEL-1 gene. Arrays comprising the novel probes immobilized on a support for hybridization analysis and methods for TBC detection using the probes are also provided.