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36 results about "GroEL" patented technology
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GroEL belongs to the chaperonin family of molecular chaperones, and is found in many bacteria. It is required for the proper folding of many proteins. To function properly, GroEL requires the lid-like cochaperonin protein complex GroES. In eukaryotes the proteins Hsp60 and Hsp10 are structurally and functionally nearly identical to GroEL and GroES, respectively.
ELISA kit for detecting Salmonella pullorum antibody
ActiveCN103995126AReduce stressImprove featuresBiological material analysisBiological testingAntigenElisa kit
An ELISA kit for detecting a Salmonella pullorum antibody is established by screening Salmonella pullorum dominant antigen GroEL through an immunoprecipitation technology, expressing GroEL recombinant protein through utilizing a prokaryotic expression vector, and utilizing an antigen protein. The kit can reduce the response of chicken in the detection process of the Salmonella pullorum antibody, and can improve the detection specificity and the sensitivity.
Owner:WENS FOOD GRP CO LTD
Mutant strain and engineering bacterium of arthrobacter simplex with stress tolerence
ActiveCN107988092AStrong toleranceImprove toleranceBacteriaHydrolasesHigh concentrationEndurance capacity
The invention belongs to the field of genetic breeding, and particularly relates to a mutant strain of arthrobacter simplex with stress tolerence and a genetically engineered bacterium constructed bythe mutant strain. The survival rate of the mutant strain under the impact conditions of 16% ethyl alcohol and 20% methyl alcohol is increased by 3.74 times and 2.10 times respectively compared with the original strain; the endurance capacity of engineering bacteria, overexpressing the genes of groEl, dnaK, recA, uvrD, katG, sod or treS from the mutant strain, to high-concentration organic solvents is obviously improved, and the endurance capacity to salt pressure and oxidative pressure is expresses to be higher. When the engineering bacteria are applied to steride C1,2 dehydrogenation reaction, the ethanol concentration in a system can be increased to 8 to 10%, the substrate CA concentration can be increased to 6 to 8g / L, the product yield can be improved by 0.41 to 3.56 times, and a goodbasis is established for the subsequent tolerance mechanism research and bacterial strain molecular modification and application.
Owner:TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
Application of Brucella GroEL protein and method for recombinant expression of Brucella GroEL protein
The invention discloses an application of Brucella GroEL protein and a method for recombinant expression of Brucella GroEL protein, in particular an application of Brucella GroEL protein used as a protective antigen in preparation of Brucella subunit vaccines, and a process for recombinant expression of the Brucella protective antigen GroEL. The experiment proves that the Brucella GroEL protein can be combined with adjuvants for immunizing to induce generation of specific antibodies and excite cell immunity, and the Brucella GroEL protein has a better immune protection effect on Brucella and can be used for preparing Brucella subunit vaccines. The Brucella GroEL protein disclosed by the invention plays an important role in epidemic prevention of Brucella, and has wide application prospects.
Owner:INST OF PLA FOR DISEASE CONTROL & PREVENTION
Novel method for realizing secreting expression of exogenous protein by using novel transfer signal
The invention belongs to the field of molecular biology, relates to a novel method for realizing the secreting expression of an exogenous protein in bacillus subtilis168 by using former 50 amino acids of bacillus subtilis168 molecular chaperone GroEl, enolase Eno and flagellar protein Hag, and specifically provides a novel method for realizing the secreting expression of an exogenous protein by fusing a transfer signal in the exogenous protein.
Owner:JIANGNAN UNIV
Fusion protein and encoding gene and preparation method of fusion protein as well as pharmaceutical composition and preparation method of pharmaceutical composition
ActiveCN103880962AHigh drug loading efficiencyGood drug releaseMacromolecular non-active ingredientsHybrid peptidesNucleotideBiocompatibility Testing
The invention discloses a fusion protein, the amino acid sequence of which contains an iRGD peptide sequence shown as SEQ ID No:1 and a molecular chaperone GroEL sequence shown as SEQ ID No:2. The invention further discloses an encoding gene of the fusion protein, the sequence of which is a nucleotide sequence capable of encoding the fusion protein. The invention further discloses a preparation method of the fusion protein. The preparation method comprises the following step of expressing the encoding gene in a bacterial strain to obtain the fusion protein. A method of preparing a pharmaceutical composition comprises the following steps of contacting the pharmaceutical compound with the fusion protein disclosed by the invention to obtain a contacted material in the presence of a solvent. The invention further discloses the pharmaceutical composition prepared by the method of preparing the pharmaceutical composition. The fusion protein provided by the invention can stably load a hydrophobic drug, and is high in drug-carrying efficiency and good in drug release effect. The pharmaceutical composition prepared by the fusion protein provided by the invention has the advantages of good biocompatibility and good drug release effect.
Owner:THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
Protein separation device
InactiveUS20090093061A1Minimal steric hindranceBioreactor/fermenter combinationsBiological substance pretreatmentsHSP60Chaperonin
The invention provides a protein separation device comprising a chaperone protein immobilised on a substrate. In one embodiment, the chaperone protein is an Hsp60 chaperone, preferably a group one chaperone, preferably GroEL. The invention also provides a method for isolating a protein from a biological sample using a protein separation device of the invention.
Owner:AGENCY FOR SCI TECH & RES
Separation, identification and applications of bacterial cellulose production strain
ActiveCN107488607AFast film productionEasy to get materialsBacteriaMicrobiological testing/measurementMicroorganismHousekeeping gene
The invention belongs to the technical field of microorganisms, and particularly relates to separation, identification and applications of a bacterial cellulose production strain, wherein the strain is isolated from corrupted fruits, and has characteristics of rapid film production and high film yield. According to the present invention, the isolated HEC-004 strain is subjected to molecular level identification by comprehensively using 16S rDNA and 3 housekeeping genes such as dnaK, groEL and rpoB, and the results determine that the isolated strain is Gluconacetobacter nataicola, is preserved in the China general microbiological culture collection center, and has the preservation number of CGMCC No.11588.
Owner:HEC PHARMA CO LTD
Kit for simultaneously detecting avian leukosis virus antibody and salmonella pullorum antibody
The invention relates to the technical field of biology and in particular relates to a kit for simultaneously detecting an avian leukosis virus antibody and a salmonella pullorum antibody. An antigencombination of the kit comprises p27 protein, gp85 protein and GroEL-delta8-1 protein. Avian leukosis virus capsid protein p27, prokaryotic expression protein of envelope protein gp85, and prokaryoticexpression protein of truncated GroEL-delta8-1 of a salmonella pullorum dominant antigen are used as a coating antigen to develop an ELISA (Enzyme-linked Immunosorbent Assay) kit capable of simultaneously detecting the avian leukosis virus antibody and the salmonella pullorum antibody. Compared with a current common kit for independently detecting avian leukosis or pullorum disease, the kit provided by the invention has the effect of simultaneously detecting the avian leukosis or the pullorum disease, and the detection and purification work of the avian leukosis and the pullorum disease can be extremely alleviated.
Owner:CHINA AGRI UNIV
Kit for simultaneously detecting avian leukosis virus antibody and salmonella pullorum antibody
ActiveCN108709995AWork lessEase the work of decontaminationMaterial analysisLeucosisAvian leukosis viruses
The invention relates to the technical field of animal epidemic disease detection and in particular relates to a kit for simultaneously detecting an avian leukosis virus antibody and a salmonella pullorum antibody. The kit comprises: P27 protein, GroEL-delta8-1 protein, an enzyme labeled second antibody, BSA (Bovine Serum Albumin), coating liquid, confining liquid, a color developing solution anda stopping solution. The invention develops an indirect ELISA (Enzyme Linked Immunosorbent Assay) kit capable of simultaneously detecting the avian leukosis virus antibody and the salmonella pullorumantibody by taking prokaryotic expression protein of a conserved region P27 of an avian leukosis virus and prokaryotic expression protein of truncated protamine GroEL-delta8-1 of a dominant antigen GroEL of salmonella pullorum as coating antigens; the kit provided by the invention can be used for simultaneously detecting avian leucosis and pullorosis; chickens which are detected to be positive areeliminated so that the aim of simultaneously purifying is realized and the clinical detection and purification work is extremely alleviated.
Owner:CHINA AGRI UNIV
Preparation method of leptospira interrogans MAP vaccine
PendingCN110478478AGood antigenicityImprove responseAntibacterial agentsBacterial antigen ingredientsSide effectSpiroplasma
The invention discloses a dominant T-and B-cell T-B combined multivalent antigen epitope fusion gene and a prokaryotic expression system thereof by constructing leptospira interrogans LipL21, LipL32,groEL and loa22 proteins. The MAP vaccine is prepared by the following steps of: expressing a multiple antigen peptide (MAP) consisting of antigen epitopes, and artificially crosslinking the MAP witha high polymer core carrier. The prepared MAP vaccine is has good immune effect, is low-cost, and is capable of effectively inducing cellular immunity. Toxic and side effects of the vaccine are reduced, safety is improved, and immune pertinence is enhanced.
Owner:ZHEJIANG MEDICAL COLLEGE
Bacterial cellulose producing strain, screening method and preparation of bacterial cellulose
The invention discloses a bacterial cellulose producing strain and an identification method thereof. The strain is named as Komagataeibacter sp.171129Z2-3, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.17276. The strain is gram-negative and short-rod-shaped bacteria, has the characteristics of wide selection range of a carbon source and a nitrogen source, high growth speed, strong thallus activity, strong cellulose production capacity and the like, can produce a large amount of bacterial cellulose during static culture and shake culture, and can achieve the yields of 5.12 g / L and 2.571 g / L after 4 days of fermentation respectively. Through physiological and biochemical characteristics, 16SrDNA, dnaK, groEL and rpoB gene sequence analysis and genome average nucleotide index (ANI) analysis, the strain is determined to be foal bacillus. The strain has a good implementation prospect in the aspect of industrial production of the bacterial cellulose.
Owner:EAST CHINA NORMAL UNIV
Preparation method and application of protein nanocage stabilized Pickering emulsion
ActiveCN109679114AGood monodispersityGuaranteed stabilityPeptide preparation methodsDepsipeptidesBiocompatibility TestingPickering emulsion
The invention provides a preparation method of protein nanocage stabilized Pickering emulsion in order to overcome defects that nano-particle stabilized Pickering emulsion is poor in biocompatibilityand biodegradability and common bioorganic molecules are poor in surface activity, have insufficient monodispersity and wetting performance and are easily affected by environmental conditions, and belongs to the field of Pickering emulsion. According to a specific scheme, a buffer solution of a GroEL protein nanocage is prepared, and mass fraction of GroEL is controlled to be 0.05wt%-0.45wt%; an oil-phase substance and water are mixed in a certain proportion, and the range of a volume ratio of the oil-phase substance to water is controlled to be 0.11-0.50; the GroEL solution is added to an oil-water mixture dropwise, meanwhile, the mixture is stirred, ultrasonic treatment is performed, and crude emulsion is enabled to be homogenized. The invention further provides an application of the Pickering emulsion as a bioactive substance for carrier encapsulating or transport. The Pickering emulsion prepared on the basis of the unique structure and excellent interfacial activity of GroEL has the advantages of good stability, long quality guarantee period, high biocompatibility and simple preparation process and is environmentally friendly.
Owner:QINGDAO GUGAO BIOTECH CO LTD
Primer pairs for detecting orientia tsutsugamushi and detection method using the same
ActiveCN103080312AImprove diagnostic accuracyExcellent diagnostic accuracyMicrobiological testing/measurementDNA/RNA fragmentationCost effectivenessOrientia tsutsugamushi
The present invention relates to a diagnosis method of scrub typhus, more precisely, to a diagnosis method of scrub typhus by detecting Orientia tsutsugamushi, the cause of scrub typhus, comprising the following steps: preparing primer pairs based on the 16s ribosomal gene sequence of Orientia tsutsugamushi; and detecting Orientia tsutsugamushi using the prepared primer pairs. The diagnosis method of the present invention not only demonstrates higher diagnostic accuracy than other conventional methods amplifying and analyzing 47kDa, 57kDa, and groEL, but also is very simple and easy,suggesting that it is excellent in the aspect of cost-effect.
Owner:金东民
Method of immunizing animal, composition for immunization, method for producing antibody, method for producing hybridoma and method for producing monoclonal antibody
ActiveUS20090041761A1Easy to produceProduce efficientlyAntibacterial agentsGenetic material ingredientsAntigenMonoclonal antibody
It is an object of the present invention to a method whereby a humoral immune response is induced more efficiently in producing an antibody against an antigen protein by gene immunization. A fusion gene composed of a gene encoding the full-length of a part of the antigen protein or a gene encoding a chaperonin subunit or a chaperonin subunit linkage linked thereto is administered to express the fusion gene in the animal, thereby inducing a humoral immune response to an antigen protein by administering. An example of the chaperonin includes Escherichia coli GroEL. There is also provided with a composition for immunization, a method for producing an antibody, a method for producing a hybridoma, and a method for producing a monoclonal antibody.
Owner:SEKISUI CHEM CO LTD
Heme-GroEL complex as well as preparation method and application thereof
InactiveCN109468294AImprove stabilityHigh activityMicrobiological testing/measurementBiological material analysisPeroxidaseHeme
The invention discloses a heme-GroEL complex and a preparation method and application thereof. The heme-GroEL complex is provided with two back-to-back cavities. The inner surface at an opening of each cavity is provided with seven hydrophobic grooves perpendicular to the center line of the cavity. Each cavity can be combined with a plurality of monomer heme molecules through hydrophobic interaction. The heme-GroEL complex is good in stability, has the significant catalase activity, can be used instead of natural peroxidase, can be used for chromogenic detection of hydrogen peroxide or glucose, and can be also used for detection or oxidation removal of phenolic pollutants.
Owner:QINGDAO GUGAO BIOTECH CO LTD
Primer pairs for detecting orientia tsutsugamushi and detection method using the same
ActiveCN103080312BImprove diagnostic accuracyExcellent diagnostic accuracyMicrobiological testing/measurementDNA/RNA fragmentationCost effectivenessOrientia tsutsugamushi
Owner:金东民
Mutant strains and engineering bacteria of Arthrobacter simplex with stress tolerance
The invention belongs to the field of genetic breeding, and in particular relates to a simple Arthrobacter mutant strain with stress tolerance and a genetic engineering bacterium constructed therefrom. Among them, the survival rate of the mutant strains under 16% ethanol and 20% methanol shock conditions increased by 3.74 times and 2.10 times respectively compared with the starting strain; overexpression of groEL, dnaK, recA, uvrD, katG, sod or treS genes from the mutant strains The tolerance of the engineered bacteria to high concentrations of organic solvents is significantly improved, and they also show higher tolerance to salt stress and oxidative stress. The above engineered bacteria are used in the steroid C1,2 dehydrogenation reaction to put The ethanol concentration was increased to 8-10%, the substrate CA concentration was increased to 6-8g / L, and the product yield was increased by 0.41-3.56 times, which laid a good foundation for the subsequent research on the resistance mechanism and the molecular transformation and application of strains.
Owner:TIANJIN UNIV OF SCI & TECH
A kit for simultaneously detecting antibodies against avian leukosis virus and Salmonella pullorum
Owner:CHINA AGRI UNIV
Phage Phi X174 lytic cell protein E and application thereof
InactiveCN108624545AFacilitated releaseEasy to operateBacteriaVirus peptidesRecombinant escherichia coliBacteriophage
The invention discloses a phage Phi X174 lytic cell protein E and application thereof and belongs to the field of genetic engineering. The method in the invention comprises the following steps: takingrecombinant Escherichia coli E. coli (PAI / GroELS) as an original strain, introducing a phage Phi X174 lytic cell protein E, producing holes in the surfaces of recombinant Escherichia coli cells, andreleasing linoleate isomerase from the cells by utilizing osmotic pressure differences inside and outside the cells. Finally, by optimizing the release conditions, 81% of linoleate isomerase in the cells is released out of the recombinant Escherichia coli cells.
Owner:JIANGNAN UNIV
Application of heat shock protein groel in preparation of reagents for detection of Helicobacter pylori
ActiveCN108287244BImprove the detection rateUndisturbedBiological testingBiotechnologyH pylori testing
The invention relates to the technological field of biotechnology, and especially relates to an application of a heat shock protein groEL to preparation of a reagent for detecting Helicobacter pylori.The application of the heat shock protein groEL to a marker for detecting Helicobacter pylori is provided; experiments confirm that heat shock protein groEL is used as the marker for detecting Helicobacter pylori, detection rate of Helicobacter pylori virulence type is greatly improved, the accuracy rate reaches 98% or above, the maker is not interfered by non-virulence type helicobacter pylori or other pathogens, and the maker has good specificity.
Owner:SHENZHEN BLOT BIOTECH
Method of immunizing animal, composition for immunization, method for producing antibody, method for producing hybridoma and method for producing monoclonal antibody
It is an object of the present invention to a method whereby a humoral immune response is induced more efficiently in producing an antibody against an antigen protein by gene immunization. A fusion gene composed of a gene encoding the full-length of a part of the antigen protein or a gene encoding a chaperonin subunit or a chaperonin subunit linkage linked thereto is administered to express the fusion gene in the animal, thereby inducing a humoral immune response to an antigen protein by administering. An example of the chaperonin includes Escherichia coli GroEL. There is also provided with a composition for immunization, a method for producing an antibody, a method for producing a hybridoma, and a method for producing a monoclonal antibody.
Owner:SEKISUI CHEM CO LTD
A kit for simultaneous detection of avian leukemia virus antibodies and Salmonella pullorum antibodies
The invention relates to the technical field of biology and in particular relates to a kit for simultaneously detecting an avian leukosis virus antibody and a salmonella pullorum antibody. An antigencombination of the kit comprises p27 protein, gp85 protein and GroEL-delta8-1 protein. Avian leukosis virus capsid protein p27, prokaryotic expression protein of envelope protein gp85, and prokaryoticexpression protein of truncated GroEL-delta8-1 of a salmonella pullorum dominant antigen are used as a coating antigen to develop an ELISA (Enzyme-linked Immunosorbent Assay) kit capable of simultaneously detecting the avian leukosis virus antibody and the salmonella pullorum antibody. Compared with a current common kit for independently detecting avian leukosis or pullorum disease, the kit provided by the invention has the effect of simultaneously detecting the avian leukosis or the pullorum disease, and the detection and purification work of the avian leukosis and the pullorum disease can be extremely alleviated.
Owner:CHINA AGRI UNIV
An elisa kit for detecting antibodies against Salmonella pullorum
ActiveCN103995126BReduce stressImprove featuresBiological material analysisBiological testingAntigenElisa kit
An ELISA kit for detecting a Salmonella pullorum antibody is established by screening Salmonella pullorum dominant antigen GroEL through an immunoprecipitation technology, expressing GroEL recombinant protein through utilizing a prokaryotic expression vector, and utilizing an antigen protein. The kit can reduce the response of chicken in the detection process of the Salmonella pullorum antibody, and can improve the detection specificity and the sensitivity.
Owner:WENS FOODSTUFF GRP CO LTD
Enzyme and bacterium functional bag for rapidly degrading soybean meal antigen and application of enzyme and bacterium functional bag
The invention belongs to the technical field of feed biology, and discloses an enzyme and bacterium functional bag capable of rapidly degrading soybean meal antigens and application of the enzyme and bacterium functional bag. The functional package comprises alkaline protease AlkP-S1mut subjected to triple mutation through an S1 pocket and thermophilic probiotic bacillus licheniformis BL-TH01, the alkaline protease AlkP-S1mut and the thermophilic probiotic bacillus licheniformis BL-TH01 are loaded in a microporous starch-sodium alginate composite carrier and have a synergistic effect at the temperature of 30-45 DEG C, and the degradation rate of beta-conglycinin is larger than or equal to 95% within 2 hours. A molecular chaperone GroEL-BL is used for inducing antigen conformation to loosen and exposing restriction enzyme cutting sites, and mutation protease is accurately hydrolyzed, so that nutrient loss and bitter taste generation are avoided. According to the invention, a high-efficiency, energy-saving and high-fidelity bean pulp antigen degradation system is constructed through a dual strategy of genetic engineering modification and microbial synergy. The system breaks through the natural limitation of a single enzyme preparation, realizes normal form transformation from passive waiting for substrate exposure to active induction of substrate opening, and provides a technical solution with economical efficiency and sustainability for the feed industry.
Owner:JINAN TIANTIANXIANG
Isolation, Identification and Application of a Bacterial Cellulose-Producing Strain
ActiveCN107488607BFast film productionEasy to get materialsBacteriaMicrobiological testing/measurementBiotechnologyMicroorganism
Owner:HEC PHARMA CO LTD
A genetically engineered bacterium with high expression of clostridial protease and application thereof
The application belongs to the technical field of genetic engineering, and particularly relates to a genetically engineered bacterium with high expression of clostridial protease and application thereof. The genetically engineered bacterium provided by the application co-expresses clostridial protease and a molecular chaperone; the molecular chaperone is GroEL and KAR2; the nucleotide sequence of the clostridial protease is shown as SEQ ID NO. 2; and the molecular chaperone has the nucleotide sequences shown as SEQ ID NO. 6 and SEQ ID NO. 10. The genetically engineered bacterium with co-expression of clostridial protease and a molecular chaperone provided by the application enables efficient expression of Arg-C in a soluble form, thereby efficiently and stably producing Arg-C. The obtained Arg-C can decompose complex proteins into specific peptide segments, so that the presence and content of a marker can be more accurately determined.
Owner:YINJIA (SHANGHAI) BIOMEDICAL TECH CO LTD
Construction method and application of canine caifn-α gene expression plasmid
InactiveCN102277372ABreed fastShort fermentation periodMicroorganism based processesFermentationYeastShuttle vector
The present invention is based on the shuttle vector between radioresistant bacteria and Escherichia coli, and is recombined with the active groEL promoter and canine CaIFN-α gene fragment derived from the radioresistant bacteria Deinococcus radiodurans to construct a plasmid with CaIFN-α expression activity. The radioresistant bacterium Deinococcus grandis was used as the host, induced by -ray irradiation, etc., to efficiently express and produce canine CaIFN-α with biological activity. The present invention better solves the complex renaturation operation required for the expression of canine CaIFN-α in Escherichia coli, the instability of plasmid transformants in yeast expression, the high cost of expression and production in mammalian cultured cells, and the need for expression in silkworm larvae. Due to seasonal restrictions, the disadvantage of not being able to produce on an annual basis.
Owner:ZHEJIANG UNIV
Streptococcus agalactiae amplification primer group, application and detection method of streptococcus agalactiae
ActiveCN112094929ARapid responseLarge amount of productMicrobiological testing/measurementMicroorganism based processesMicroorganismNano biosensor
The invention relates to a streptococcus agalactiae amplification primer group, an application and a detection method of streptococcus agalactiae, and belongs to the technical field of microbiologicaldetection. The detection method of streptococcus agalactiae comprises the following steps: 1) designing an MCDA amplification primer group of specific genes groEL: CP1, CP2, F1, F2, D1 *, D2, C1, C2,R1 * and R2; 2) in a reaction system comprising the amplification primer group, taking a genome of a streptococcus agalactiae sample to be detected as a template, and performing MCDA constant-temperature amplification to obtain an amplification product; and 3) detecting the amplification product in the step 2) by using a gold nano biosensor to obtain a detection result. According to the method, on the basis of multi-cross constant-temperature amplification and in combination with a nano-biological detection technology, the streptococcus agalactiae can be directly, quickly, sensitively and highly-specifically detected and identified from clinical samples.
Owner:十堰市太和医院(湖北医药学院附属医院)
Novel method for realizing secreting expression of exogenous protein by using novel transfer signal
InactiveCN102925475BIncrease secretionReduce secretionBacteriaMicroorganism based processesFlagellar proteinSecretion expression
The invention belongs to the field of molecular biology, relates to a novel method for realizing the secreting expression of an exogenous protein in bacillus subtilis168 by using former 50 amino acids of bacillus subtilis168 molecular chaperone GroEl, enolase Eno and flagellar protein Hag, and specifically provides a novel method for realizing the secreting expression of an exogenous protein by fusing a transfer signal in the exogenous protein.
Owner:JIANGNAN UNIV
Protein separation device
InactiveUS8013129B2Minimal steric hindranceBioreactor/fermenter combinationsBiological substance pretreatmentsHSP60Chaperonin
The invention provides a protein separation device comprising a chaperone protein immobilized on a substrate. In one embodiment, the chaperone protein is an Hsp60 chaperone, preferably a group one chaperone, preferably GroEL. The invention also provides a method for isolating a protein from a biological sample using a protein separation device of the invention.
Owner:AGENCY FOR SCI TECH & RES