34 results about "GroEL" patented technology

The present invention relates to a diagnosis method of scrub typhus, more precisely, to a diagnosis method of scrub typhus by detecting Orientia tsutsugamushi, the cause of scrub typhus, comprising the following steps: preparing primer pairs based on the 16s ribosomal gene sequence of Orientia tsutsugamushi; and detecting Orientia tsutsugamushi using the prepared primer pairs. The diagnosis method of the present invention not only demonstrates higher diagnostic accuracy than other conventional methods amplifying and analyzing 47kDa, 57kDa, and groEL, but also is very simple and easy,suggesting that it is excellent in the aspect of cost-effect.

The invention belongs to the field of genetic breeding, and in particular relates to a simple Arthrobacter mutant strain with stress tolerance and a genetic engineering bacterium constructed therefrom. Among them, the survival rate of the mutant strains under 16% ethanol and 20% methanol shock conditions increased by 3.74 times and 2.10 times respectively compared with the starting strain; overexpression of groEL, dnaK, recA, uvrD, katG, sod or treS genes from the mutant strains The tolerance of the engineered bacteria to high concentrations of organic solvents is significantly improved, and they also show higher tolerance to salt stress and oxidative stress. The above engineered bacteria are used in the steroid C1,2 dehydrogenation reaction to put The ethanol concentration was increased to 8-10%, the substrate CA concentration was increased to 6-8g / L, and the product yield was increased by 0.41-3.56 times, which laid a good foundation for the subsequent research on the resistance mechanism and the molecular transformation and application of strains.

The invention relates to the technical field of animal epidemic disease detection and in particular relates to a kit for simultaneously detecting an avian leukosis virus antibody and a salmonella pullorum antibody. The kit comprises: P27 protein, GroEL-delta8-1 protein, an enzyme labeled second antibody, BSA (Bovine Serum Albumin), coating liquid, confining liquid, a color developing solution anda stopping solution. The invention develops an indirect ELISA (Enzyme Linked Immunosorbent Assay) kit capable of simultaneously detecting the avian leukosis virus antibody and the salmonella pullorumantibody by taking prokaryotic expression protein of a conserved region P27 of an avian leukosis virus and prokaryotic expression protein of truncated protamine GroEL-delta8-1 of a dominant antigen GroEL of salmonella pullorum as coating antigens; the kit provided by the invention can be used for simultaneously detecting avian leucosis and pullorosis; chickens which are detected to be positive areeliminated so that the aim of simultaneously purifying is realized and the clinical detection and purification work is extremely alleviated.

The invention relates to the technical field of biology and in particular relates to a kit for simultaneously detecting an avian leukosis virus antibody and a salmonella pullorum antibody. An antigencombination of the kit comprises p27 protein, gp85 protein and GroEL-delta8-1 protein. Avian leukosis virus capsid protein p27, prokaryotic expression protein of envelope protein gp85, and prokaryoticexpression protein of truncated GroEL-delta8-1 of a salmonella pullorum dominant antigen are used as a coating antigen to develop an ELISA (Enzyme-linked Immunosorbent Assay) kit capable of simultaneously detecting the avian leukosis virus antibody and the salmonella pullorum antibody. Compared with a current common kit for independently detecting avian leukosis or pullorum disease, the kit provided by the invention has the effect of simultaneously detecting the avian leukosis or the pullorum disease, and the detection and purification work of the avian leukosis and the pullorum disease can be extremely alleviated.

An ELISA kit for detecting a Salmonella pullorum antibody is established by screening Salmonella pullorum dominant antigen GroEL through an immunoprecipitation technology, expressing GroEL recombinant protein through utilizing a prokaryotic expression vector, and utilizing an antigen protein. The kit can reduce the response of chicken in the detection process of the Salmonella pullorum antibody, and can improve the detection specificity and the sensitivity.

The invention belongs to the technical field of microorganisms, and in particular relates to the isolation, identification and application of a bacteria strain producing bacterial cellulose. The strain is isolated from spoiled fruit and has the characteristics of rapid film production and high film production. The present invention comprehensively uses 16S rDNA, and three housekeeping genes dnaK, groEL, and rpoB to carry out molecular analysis on the isolated HEC‑004 strain. Through horizontal identification, it was determined that the isolated strain was Gluconacetobacter nataicola, which has been preserved in the China General Microorganism Culture Collection Management Center with the preservation number: CGMCC No.11588.

The invention belongs to the field of molecular biology, relates to a novel method for realizing the secreting expression of an exogenous protein in bacillus subtilis168 by using former 50 amino acids of bacillus subtilis168 molecular chaperone GroEl, enolase Eno and flagellar protein Hag, and specifically provides a novel method for realizing the secreting expression of an exogenous protein by fusing a transfer signal in the exogenous protein.

The invention provides a protein separation device comprising a chaperone protein immobilized on a substrate. In one embodiment, the chaperone protein is an Hsp60 chaperone, preferably a group one chaperone, preferably GroEL. The invention also provides a method for isolating a protein from a biological sample using a protein separation device of the invention.