32results about How to "Large amount of product" patented technology

The invention discloses application of heat-resistant beta-glucosidase and mutants thereof. The mutants of the heat-resistant beta-glucosidase G224T, N223S and N223S / G224T have the amino acid sequences shown in SEQ: ID NO. 1, SEQ: ID NO. 2 and SEQ: ID NO. 3; the heat-resistant beta-glucosidase and the mutants thereof can be used for preparing quercetin through enzymolysis of quercetin glycoside. The preparation of the quercetin through the heat-resistant beta-glucosidase and the mutants thereof has the advantages of high conversion efficiency, few reaction byproducts, easy separation and purification of the product, less chemical pollution, and high yield and high purity of the obtained quercetin product, and the like.

The invention provides a method for preparing a steroid drug intermediate employing bioconversion phytosterol. The method comprises the following steps: 1) providing turbid liquid of phytosterol; 2) providing a resting cell of a microbial strain for converting the phytosterol into a steroid drug intermediate; 3) mixing the turbid liquid of the phytosterol with the resting cell, and adding beta-cyclodextrin or chemically modified beta-cyclodextrin to evenly mix, and cultivating to finish conversion; 4) centrifuging the conversion liquid obtained in the step 3), separating supernatant from thallus, separating to obtain the steroid drug intermediate from the supernatant by solvent extraction. By adopting the method provided by the invention, the sterol feeding concentration is improved, the target product quantity is increased, simple separation of the thallus, a cosolvent and a product is achieved when the conversion lasting time is shortened, repeated use of the thallus and the cosolvent for a plurality of times is achieved, the production cost is greatly saved, and the method is applicable to industrial production.

The invention discloses an application of heat-resisting beta-glucosidase mutants N223T, G224F and N223T / G224F to transforming arctiin into arctigenin. Amino acid sequences of the heat-resisting beta-glucosidase mutants N223T, G224F and N223T / G224F are indicated in SEQ: ID NO.1, ID NO.2 and ID NO.3. The arctiin is hydrolyzed to prepare the arctigenin by the aid of heat-resisting beta-glucosidase and the mutants thereof, and as compared with wild-type enzyme, the heat-resisting beta-glucosidase has the advantages of high transformation efficiency, fewer reaction by-products, high yield and purity, easiness in separation and purification, less chemical pollution and the like.

The invention discloses a method for controlling synthesis of molybdenum disulfide particles in a mixed solvent system, and belongs to preparation methods of transition metal sulfide materials. The method for controlling synthesis of the molybdenum disulfide particles in the mixed solvent system includes the steps: taking a molybdenum source and a sulfur source as reaction raw materials in a hydrothermal system to prepare the molybdenum disulfide micro-nano-particles; effectively regulating microstructures of the molybdenum disulfide particles by different types of mixed solvents; adding surfactants to control the sizes and the dispersion degree of the molybdenum disulfide particles; controlling synthesis of the molybdenum disulfide micro-nano-particles by the mixed solvents and the surfactants. The method has the advantages that used raw materials are low in price and easy to obtain, the synthesis method is simple in operation, experimental results are high in repeatability, productsis large in quantity and high in purity, the method can be applied to large-scale production, and the prepared molybdenum disulfide materials have wide application prospects in the fields of lithium-ion batteries, sodium-ion batteries, photocatalysis and the like.

The invention provides amplification primer sets of Klebsiella pneumoniae, application and a method for detecting the Klebsiella pneumoniae. The method for detecting the Klebsiella pneumoniae comprises the following steps: 1) extracting a genome of a Klebsiella pneumoniae sample to be detected; 2) providing the following specific gene rcsA amplification primer sets of the Klebsiella pneumoniae: CP1, CP2, F1, F2, D1, C1, R1, D2, C2, R2, C1*, C2*, D1* and D2*; 3) using genomic nucleic acid of the Klebsiella pneumoniae sample to be detected as a template for isothermal amplification under a multi-crossover isothermal amplification reaction system comprising the amplification primer sets to obtain an amplification product; 4) detecting the amplification product of the step 3) by using a gold nanobiosensor. The method provided by the invention uses multi-crossover isothermal amplification as a basis, is combined with a nanometer biological detection technology, and can rapidly, sensitivelyand highly specifically identify the Klebsiella pneumoniae.

The invention discloses a method for preparing a flower-like ternary metal oxide semiconductor ZnSnO3, and the method is as follows: adding an ethanol solution A of SnCl4 (0.2M) into a deionized watersolution B of ZnSO4 (0.1M) and triethanolamine (10-30% by weight) (wherein the molar ratio of SnCl4 to ZnSO4 is 1:1), and stirring continuously; adding a 0.4M NaOH solution dropwise to an A and B mixed solution according to a volume ratio of 5:3, continuing stirring, standing for 6 hours to produce a solid flower-like precipitate of ZnSn(OH)6; or on the basis of the solution, adding a 2M NaOH solution (pH=10-12) dropwise while stirring, continuing stirring, standing for 6 hours to produce a hollow flower-like precipitate of ZnSn(OH)6 when the molar ratio of NaOH to ZnSn (OH) 6 is 40:1; separately cleaning and drying the hollow flower-like precipitate or the solid flower-like precipitate to obtain corresponding ZnSn(OH)6 powder, and calcining the powder at 500 DEG C for 2 h to obtain a micron-scale solid or hollow flower-like structure ZnSnO3. The method is simple, and the product is large in quantity and uniform in distribution, and has a large specific surface area.

The invention discloses application of a heat-resistant alpha-glucuronidase polypeptide fusion body in preparation of glucuronic acid. An amino acid sequence of the fusion body is as shown in SEQ ID NO.1. According to the invention, seven amino acid residues are fused at the C end of heat-resistant alpha-glucuronidase protein to form a brand-new polypeptide fusion body, the specific activity of the obtained polypeptide fusion body is improved by 297% compared with that of wild type enzyme TmAguA, the Kcat and Kcat / Km of a corresponding hydrolyzed 4-O-methyl glucuronic acid xylo-oligosaccharidesubstrate are improved by 356% and 363% respectively, and the stability is improved by 21.1%-65.7% compared with that of the wild type enzyme; the fusion body is used for preparing the glucuronic acid, and compared with the wild type enzyme, the fusion body has the advantage that the yield of the glucuronic acid prepared by converting beechwood xylan through the polypeptide fusion body is increased by 21.6-60.3%; and the fusion body has the advantages of high conversion efficiency, few reaction byproducts, high yield and purity, easiness in separation and purification, less chemical pollutionand the like.

The invention relates to a chloroplast genome of chlorella leaves, in particular to application of chloroplast of large chlorella leaves as a receptor and an expression vector of exogenous genes. The application has the following advantages: a conversion system and a conversion method are integrated, processes are simple and convenient, conversion efficiency is high, and culture conditions are simple; the exogenous genes have wide receiving range and large yield, and can be expressed in a shorter time; and the cost is low, and the application effect is good.

The invention provides an amplification primer group of pseudomonas aeruginosa, applications of the amplification primer group, and a detection method of pseudomonas aeruginosa. The detection method of pseudomonas aeruginosa comprises the following steps: (1) extracting the genome of a pseudomonas aeruginosa sample to be detected; (2) providing the specific gene oprL amplification primer group ofpseudomonas aeruginosa: CP1, CP2, F1, F2, D1, C1, R1, D2, C2, R2, C1*, C2* as well as D1* and D2*; (3) in a multi-crossing isothermal amplification reaction system comprising the amplification primergroup, carrying out isothermal amplification by adopting the genome nucleic acid of the pseudomonas aeruginosa sample to be detected as the template, thus obtaining the amplification product; and (4)detecting the amplification product of the step (3) by adopting a gold nanobiosensor. For the method provided by the invention, the multi-crossing isothermal amplification is adopted as the basis, thenanometer biological detection technology is combined, and pseudomonas aeruginosa can be rapidly and sensitively identified with high specificity.

The invention discloses a Co3S4 ultrathin nanosheet / rGO composite structure and preparation method and application thereof. The preparation method comprises the steps of dissolving cobalt chloride hexahydrate in a solvent for stirring, adding ammonia water for reaction, adding thioacetamide for stirring, cooling to a room temperature after reaction in a high-pressure kettle, abandoning a supernatant, and cleaning a sample to obtain nanometer-structure Co3S4; and dissolving graphene oxide in the solvent with ultrasound, adding cobalt chloride hexahydrate for stirring and dissolution, dropwise and slowly adding the ammonia water, adding TAA and continuing to stir after stirring, naturally cooling to the room temperature after reaction in the high-pressure kettle, cleaning the product with water and ethyl alcohol, and freezing and drying the product for use. By addition of NH<3>.H2O and introduction of NH3 molecules, the morphology of the product is adjusted to be a two-dimensional sheet-shaped structure, and the specific area of the structure is expanded; with introduction of GO during the synthesis process, the Co3S4 nanosheet structure is dispersed, an agglomeration phenomenon of the sheet structure is prevented, and the catalytic activity, the electron transfer capability and the catalytic stability of the Co3S4 nanosheet are improved.

The invention discloses a simple preparation method of Cu2O micropowder. The simple preparation method of Cu2O micropowder comprises following steps: 1, CuCl2.2H2O and lactose are added into deionizedwater to prepare a solution, NaOH solid is added into the solution so as to obtain an alkali reaction precursor solution; 2, the alkali reaction precursor solution is subjected to oxidation-reductionreaction at normal temperature so as to obtain the Cu2O micropowder. Compared with the prior art, the beneficial effects are that: CuCl2.2H2O is taken as a copper source, lactose is taken as a reductive agent, oxidation-reduction reaction is carried out in an alkali system so as to obtain the Cu2O micropowder of a plurality of morphologys, control preparation of Cu2O micropowder from lactose andNaOH is realized, a novel method is provided for large scale preparation of cheap Cu2O. No special experiment instrument or equipment is needed; operation is simple; cost is low; and product amount islarge.

The invention discloses a preparation method of nano titanium dioxide. The preparation method comprises the following steps: (1) mixing a titanium oxide with a sodium hydroxide aqueous solution, and carrying out hydrothermal reaction to obtain nano sodium titanate with a one-dimensional structure; (2) carrying out acid exchange on the nano sodium titanate with the one-dimensional structure to obtain nano titanic acid powder with the one-dimensional structure; (3) mixing the nano titanic acid powder with the one-dimensional structure with a small amount of hydrochloric acid solution, and sealing in a reaction kettle; and (4) carrying out heat treatment on the sealed reaction kettle to obtain the nano titanium dioxide material.

The invention discloses a device and preparation method for preparing polyferric sulfate through microbial catalysis. The device is arranged in a sewage treatment plant, the device comprises a bioreactor, the bioreactor is respectively connected with a PFS product storage tank and a precipitate storage tank through a liquid outlet pipe and a discharge pipe, The PFS product storage tank is connected with an inlet of a secondary sedimentation tank through a conveying pipeline, and the secondary sedimentation tank and a water outlet are connected with the bioreactor through a water inlet pipe. An aeration device is arranged on the bioreactor, the aeration device comprises an air pipe and an aeration head, the aeration head is arranged in the bioreactor, and the other end of the air pipe is connected with an aeration system in a sewage treatment plant. The device has the advantages that a synthesis device is arranged in a sewage treatment plant, so that the prepared product is directly conveyed into the sewage treatment plant for use through a conveying pipeline, and the transportation cost is effectively saved.

The invention provides a method for detecting candida albicans by applying a multi-crossing constant-temperature amplification combined with nano bio-sensing technology. The invention provides candidaalbicans ITSII amplification primer groups: CP1, CP2, F1, F2, C1, C2, D1, D2, R1 and R2; the method is used for marking hapten on a 5' end of an amplification primer C1 in multi-crossing constant-temperature amplification and marking biotin on a 5' end of an amplification primer D1; an amplification product aiming at a candida albicans specific sequence interval ITSII of the method can be visually detected through a gold nano bio-sensor; the method is convenient, rapid, sensitive and excellent and is suitable for being popularized to clinical detection of the candida albicans.

The invention discloses a Co3S4 ultrathin nanosheet / rGO composite structure and preparation method and application thereof. The preparation method comprises the steps of dissolving cobalt chloride hexahydrate in a solvent for stirring, adding ammonia water for reaction, adding thioacetamide for stirring, cooling to a room temperature after reaction in a high-pressure kettle, abandoning a supernatant, and cleaning a sample to obtain nanometer-structure Co3S4; and dissolving graphene oxide in the solvent with ultrasound, adding cobalt chloride hexahydrate for stirring and dissolution, dropwise and slowly adding the ammonia water, adding TAA and continuing to stir after stirring, naturally cooling to the room temperature after reaction in the high-pressure kettle, cleaning the product with water and ethyl alcohol, and freezing and drying the product for use. By addition of NH<3>.H2O and introduction of NH3 molecules, the morphology of the product is adjusted to be a two-dimensional sheet-shaped structure, and the specific area of the structure is expanded; with introduction of GO during the synthesis process, the Co3S4 nanosheet structure is dispersed, an agglomeration phenomenon of the sheet structure is prevented, and the catalytic activity, the electron transfer capability and the catalytic stability of the Co3S4 nanosheet are improved.

The invention discloses application of heat-resistant beta-glucosidase and mutants thereof. The mutants of the heat-resistant beta-glucosidase G224T, N223S and N223S / G224T have the amino acid sequences shown in SEQ: ID NO. 1, SEQ: ID NO. 2 and SEQ: ID NO. 3; the heat-resistant beta-glucosidase and the mutants thereof can be used for preparing quercetin through enzymolysis of quercetin glycoside. The preparation of the quercetin through the heat-resistant beta-glucosidase and the mutants thereof has the advantages of high conversion efficiency, few reaction byproducts, easy separation and purification of the product, less chemical pollution, and high yield and high purity of the obtained quercetin product, and the like.

The invention relates to a composite material in a precious metal / titanium dioxide micro-nano structure, in particular to an optical resonance composite material in a noble metal / titanium dioxide micro-nano structure and a preparation method thereof. The material belongs to a quasi monodisperse floccular micro-nano structure grain with the grain diameter between 300 nm and 3 mum formed by self assembly by nanometer rods with the diameter between 30 and 50 nm and the length between 100 and 500 nm. The material has the main ingredients of titanium dioxide and noble metal of silver or gold, wherein the mol ratio of titanium to silver or gold is 1 / 0.05 to 0.5. The material has special optical transmission performance in the visible light wave band. The method for preparing the material is a solvent thermosynthesis method, the preparation process is simple, the yield is high, and the grain diameter, the microcosmic appearance, the structure and the performance of the grains can all realize the effective control and regulation. The accompanying drawing shows the scanning electron microscope photos of the composite material in the silver / titanium dioxide micro-nano structure when the mol ratio of the titanium to the sliver is 1 / 0.25.

The invention discloses a preparation method of beta-FeOOH nano-capsules, and relates to the technical field of preparation of nano-capsules. The method comprises the following steps: performing hydrolysis by using an aqueous solution of ferric chloride to generate beta-FeOOH nano-rods, and carrying out heating alcohol thermalmaturation on the beta-FeOOH nano-rods, glycerin and water to prepare the beta-FeOOH nano-capsules. The beta-FeOOH nano-capsules are successfully obtained by adopting an alcohol thermalmaturation technology with green and environmentally-friendly glycerin as a reaction medium and the beta-FeOOH nano-rods as a precursor. The method has the characteristics of simplicity in operation, low cost, and excellent visible light absorption performance of the product, and provides a simple and environmentally-friendly method for preparing capsule type nanoparticles.

The invention relates to the technical field of biomedicines and biology, in particular to a high-sensitivity method for screening a strain for catalyzing the conversion of levo-fosfomycin into dextral-fosfomycin, which comprises: firstly, preparing bacterial suspension of a dextral-fosfomycin sensitive strain, wherein the bacterial concentration is 10<8> to 10<10> / ml; secondly, adding the bacterial suspension of the dextral-fosfomycin sensitive strain into sterile fermentation liquid of catalyst strain to be screened, which is filtered by a filter membrane with an aperture of 0.45 mu m to remove bacteria, collecting fermentation liquid at the starting time of culture and after a certain period of culture, and measuring the absorbance of the collected fermentation liquid by using a 600-nanometer spectrophotometer; and selecting fermentation liquor of which the absorbance increases the least or less after a period of culture, and further verifying if the original strain to be screened has the capability of converting levo-fosfomycin into dextral-fosfomycin. When the method is used, the sensitivity of the method for screening the biological catalyst strain for converting levo-fosfomycin into dextral-fosfomycin is improved greatly, and the screening efficiency is improved greatly for the detection method is simple and easy to implement.

The invention discloses a device and a preparation method for preparing polyferric sulfate by microbial catalysis. The device is arranged in a sewage treatment plant, and the device includes a bioreactor. The storage tank is connected to the sediment storage tank, the PFS product storage tank is connected to the inlet of the secondary settling tank through the delivery pipeline, and the secondary settling tank and the water outlet are connected to the bioreactor through the water inlet pipe. An aeration device is installed on the bioreactor, and the aeration device includes an air pipe and an aeration head, the aeration head is inside the bioreactor, and the other end of the air pipe is connected with the aeration system in the sewage treatment plant. The advantage of the present invention is that the synthesis device is arranged in the sewage treatment plant, so that the prepared product is directly transported to the sewage treatment plant for use through the conveying pipeline, effectively saving the transportation cost.

According to the invention, nanosphere-flower-like mixed type magnesium-doped ZnSnO3 particles are prepared on the basis of a coprecipitation method, and the ZnSnO3 particles subjected to proper magnesium doping modification are the combination of nanoscale spheres and micron-scale flower-like particles. The Mg-doped ZnSnO3 powder is prepared by adopting the coprecipitation method, and the methodis simple in process, easy to operate, large in product quantity and uniform in distribution. In the preparation process, NaOH is as a precipitant, the nanoscale hollow Mg-doped ZnSnO3 is finally obtained by adjusting the pH value of a solution and assisting by an organic additive TEOA, and compared with nanoscale cubic or spherical ZnSnO3 prepared by other methods, the nanoscale Mg-doped ZnSnO3 has a relatively large specific surface area.

The present invention relates to the technical field of molecular biology, and discloses a PCR amplification system for whole genome bisulfate sequencing, and a library construction method thereof. The PCR amplification system comprises: 19 [mu]l of ultra-pure water; 15 [mu]l of a DNA template; 5 [mu]l of a reaction buffer liquid, 2.5 [mu]l of an upstream primer, 2.5 [mu]l of a downstream primer; 5 [mu]l of 2.5 mM dNTP; and 1 [mu]l of hot-start DNA polymerase. According to the present invention, with the application of the PCR amplification system in the PCR amplification step of the target fragment treated by the salt during the whole genome bisulfate sequencing process, the product concentration of the PCR amplification reaction can be improved, and the sufficient loading amount of the sequencing can be provided; the present invention further provides a bisulfate sequencing library construction method containing the PCR amplification step, wherein the sufficient loading amount of the methylation sequencing library can be constructed and the smooth operating of the whole genome bisulfate sequencing can be easily achieved through the method.

The invention relates to a streptococcus agalactiae amplification primer group, an application and a detection method of streptococcus agalactiae, and belongs to the technical field of microbiologicaldetection. The detection method of streptococcus agalactiae comprises the following steps: 1) designing an MCDA amplification primer group of specific genes groEL: CP1, CP2, F1, F2, D1 *, D2, C1, C2,R1 * and R2; 2) in a reaction system comprising the amplification primer group, taking a genome of a streptococcus agalactiae sample to be detected as a template, and performing MCDA constant-temperature amplification to obtain an amplification product; and 3) detecting the amplification product in the step 2) by using a gold nano biosensor to obtain a detection result. According to the method, on the basis of multi-cross constant-temperature amplification and in combination with a nano-biological detection technology, the streptococcus agalactiae can be directly, quickly, sensitively and highly-specifically detected and identified from clinical samples.

The invention relates to a preparation method of a CaCO3 nanotube / podophyllum composite material, specifically: first dissolving 4.0 g of o-phenanthroline carrier in 100 ml of chloroform, vigorously stirring, and then cleaning and drying TFOM and putting it into a solution system for soaking After a certain period of time, take it out, clean it with filter paper, put it in the middle of the GRC container, and form a TFOM in the middle, and a solution phase separation system on the left and right. Prepare 0.53%-0.57% sodium carbonate solution and 2.13%-2.17% calcium chloride The solution was placed on the left and right sides of the above-mentioned TFOM respectively, and the crystal growth modifier M was added according to different situations, and the pH value was adjusted to 8. After stirring at a low speed, the solution on one side of the sodium carbonate solution was taken out, and the precipitated product was obtained by centrifugation. Water, acetone, Wash each with absolute ethanol twice, and finally store the product in absolute ethanol. The present invention has stable reaction, simple operation and easy control, low cost and no pollution, easy control of appearance and structure, uniform product distribution, not easy to agglomerate, and high purity. Easy to industrialize.

The invention discloses a preparation method for a two-dimensional supported nanometer magnesium hydride hydrogen storage material. The preparation method comprises the following steps of (1) by taking a magnesium metal-contained organic compound and graphene as raw materials, under argon shield, performing ball milling at a room temperature and a hydrogen atmosphere, and performing hydrogenationon the magnesium metal-contained organic compound to obtain suspension liquid of graphene supported magnesium hydride as a reaction product; and (2) performing suction filtration and drying on the obtained reaction product to obtain the two-dimensional supported nanometer magnesium hydride hydrogen storage material. The preparation method provided by the invention has the advantages of reduced cost, simplified synthetic method, simple technological process, high production efficiency, low energy consumption, high process safety and relatively large yield; and in addition, the two-dimensional supported nanometer magnesium hydride prepared by the preparation method has high hydrogen storage capacity and excellent cycling stability, has very large potential on the aspects of hydrogen storageand conveying, fuel cells and the like and is a hydrogen storage material with great application prospect.

The invention discloses a method for controlling synthesis of molybdenum disulfide particles in a mixed solvent system, and belongs to preparation methods of transition metal sulfide materials. The method for controlling synthesis of the molybdenum disulfide particles in the mixed solvent system includes the steps: taking a molybdenum source and a sulfur source as reaction raw materials in a hydrothermal system to prepare the molybdenum disulfide micro-nano-particles; effectively regulating microstructures of the molybdenum disulfide particles by different types of mixed solvents; adding surfactants to control the sizes and the dispersion degree of the molybdenum disulfide particles; controlling synthesis of the molybdenum disulfide micro-nano-particles by the mixed solvents and the surfactants. The method has the advantages that used raw materials are low in price and easy to obtain, the synthesis method is simple in operation, experimental results are high in repeatability, productsis large in quantity and high in purity, the method can be applied to large-scale production, and the prepared molybdenum disulfide materials have wide application prospects in the fields of lithium-ion batteries, sodium-ion batteries, photocatalysis and the like.

The invention provides a preparation method of MoS2 micro-nanospheres with a hierarchical structure, and relates to the technical field of preparation of micro-nanostructured materials. The MoS2 micro-nanospheres with the hierarchical structure are prepared through a hydrothermal reaction, an S source and a Mo source are added to a PAM (polyacrylamide) aqueous solution at the room temperature andstirred uniformly, and a reaction precursor solution is obtained; the reaction precursor solution is transferred into a reaction kettle and subjected to the hydrothermal reaction, and the MoS2 micro-nanostructured material can be obtained. According to the preparation method, the MoS2 micro-nanospheres with the hierarchical structure are successfully prepared from thioacetamide or thiourea as theS source, sodium molybdate or ammonium molybdate as the Mo source and a PAM template control agent with a simple hydrothermal method. Shaping of the MoS2 particles is realized, and one new method is provided for preparation of the MoS2 material with the hierarchical structure. The preparation method is simple to operate and low in cost, and the yield of obtained products is high.

The invention discloses a method for extracting high-purity AlFeO3 powder from coal ash. The method comprises the following steps that 1, solid salicylic acid is added into a coal ash-water system, after stirring and ultrasonic reaction, standing is conducted, an amaranthine complex which suspends on the upper layer is separated out, and after the amaranthine complex is subjected to multiple washing and drying treatment, dry ferro-aluminum salisylate complex powder is obtained; 2, a dry pot containing the ferro-aluminum salisylate complex powder in the step 1 is placed in a muffle furnace, andafter calcination is conducted in the air, the AlFeO3 powder can be obtained. Compared with the prior art, the method for extracting the high-purity AlFeO3 powder from the coal ash has the beneficialeffects that controllable regeneration of the coal ash is achieved, and a new path is provided for preparation and application of the high-purity AlFeO3 powder; the method has the characters that operation is simple, the cost is low, the product quantity is large, the visible light absorption performance is excellent, and the like.