Amplification primer group of pseudomonas aeruginosa, applications of amplification primer group, and detection method of pseudomonas aeruginosa
A Pseudomonas aeruginosa, amplification primer technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganism determination/inspection, etc., can solve the problem of high cost, delayed diagnosis and treatment time, unfavorable rapid detection and Emergency detection and other problems, to achieve the effect of convenient method and increase the amount of MCDA products
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[0025] The invention solves the defects of the prior art for detecting Pseudomonas aeruginosa by providing an amplification primer set for Pseudomonas aeruginosa, its application and a detection method for Pseudomonas aeruginosa.
[0026] In order to solve the above defects, the main ideas of the embodiments of the present invention are:
[0027] The amplification primer set of Pseudomonas aeruginosa in the embodiment of the present invention is characterized in that it includes: the internal cross primer CP1 shown in SEQ ID NO: 1, the internal cross primer CP2 shown in SEQ ID NO: 2, such as Replacement primer F1 shown in SEQ ID NO: 3, replacement primer F2 shown in SEQ ID NO: 4, and amplification primers D1, C1, R1, D2, C2 and R2 shown in SEQ ID NO: 5-10 ; The 5' end of the amplification primer C1 or C2 is labeled with biotin to obtain C1* or C2*; the 5' end of the amplification primer D1 or D2 is labeled with a hapten to obtain D1* or D2*.
[0028] The embodiment of the pre...
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